BaquA.18398.a

Type IV secretion system-coupling protein virD4 (VirB type IV secretion machinery)

CENTER ID: BaquA.18398.a
ORGANISM: Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.18398.a.B2.GE36072 soluble domain 146 639
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.18398.a.B2.PW37230 soluble domain 146 639

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283165.4.peg.1155
RefSeq: YP_032630.1
UniProt: Q6FYV9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKYTKIQLAL ILMPIALGAL TIFLVPHLLS FMINDLKANH VYWYVRSEPL LVLMLVATVS LCYTLSQKLH LRKAITLVSA VFFGITALYF IGGEIKRLTP YVGQQGITWS YALKFMDPMV VFGVICGVVL LVIQVMISSP PTSKVKRAKK GIFGDASWMN LKEAAKIFPA NGQIVVGERY RVDQDGVCNI PFAPGNKTTW GKGGTAPLLT FNLDFGSTHM IFFAGSGGYK TTSTVVPTCL TYPGPIVCLD PSTEIAPMVR FARKKMGNRN VIVLDPNSLL TKNFNVLDWL LDDSVPRTQR EANIVGFSKL LLTDKKSENS SAEYFSTQAH NLLTALLAHV IFSDEYEDSE RNLKTLRAIL SQSETAVVNQ LRMIQETTPS PFIREMVGIF TEMADQTFSG VYTTASKDTQ WLSLSNYADL VCGNDFSSSD IANGKTDVFL NLPASILNSY PAIGRVIIGA FLNAMVTADG KYKKRVLFVL DEVDLLGYMN ILEEARDRGR KYGTSLMLFY QSSGQLVNHF GEAGARSWFE SCSFVSYAAI KDLQTAKDIS ERCGQMTVEV TGTNKSRGLS LGKSSYNVNY QQRALILPHE IIQEMRQDEQ IILMQGQPPL RCGRAIYFRR KEMLAAANKN RFAPQAKKN
NT Sequence
atgaaataca caaagataca gctggcgctt attttaatgc caatcgcttt aggtgcttta acgatattcc ttgtcccgca tttattgtca tttatgatca atgatttgaa agccaatcat gtttactggt atgttcgttc agagccctta ttggtgctga tgctggttgc tactgtgtca ttatgttata ccctatcgca aaagctgcac ctgcgcaaag caatcacgct ggtctccgct gtcttttttg ggatcactgc tctttacttt attggcggtg aaataaaacg cttaactccc tatgttgggc aacaagggat aacatggagc tatgctctca agttcatgga tcctatggtt gtttttggcg tcatttgtgg cgttgttctt ttagtaattc aagtcatgat aagctctccg cccacaagca aagtcaagcg tgcgaaaaaa ggaatttttg gggatgcctc atggatgaat ttaaaagaag cagcaaaaat ttttcccgcc aatggtcaaa ttgttgttgg cgaaagatac cgtgttgacc aagacggtgt gtgcaatatt ccctttgcgc ctggcaataa aacaacatgg ggaaaagggg gaacagcccc tttgcttacc tttaatcttg attttggttc aacccacatg atcttttttg ccggttctgg tgggtataaa acaacaagta cagttgttcc gacctgttta acatatccag gacctattgt ttgccttgat ccttcaacag aaattgcgcc aatggttagg tttgcgcgga aaaaaatggg caatagaaat gttattgttc tcgatcccaa ttccctctta acaaaaaatt ttaatgtact tgattggctt ttagacgata gcgtaccgcg cacacaacgt gaagctaata ttgtcggttt ttccaaactg cttcttacgg ataaaaaatc agagaattct tcggcagaat atttttcgac acaagcacat aatcttttaa ctgcgcttct tgcacatgtc atcttctctg atgaatatga agatagtgag cgcaatttaa aaacattgcg cgcgatcctt tctcagtcag aaacggctgt tgtgaatcaa ttgcgcatga ttcaagaaac aacaccttct ccttttattc gtgaaatggt tggtattttc accgaaatgg cagaccaaac cttttcaggg gtctatacaa cggcatcaaa agatacccag tggctttctt tgtccaatta cgctgatctt gtttgtggca atgatttttc gtcttcggat attgcaaatg gaaagacaga tgtttttctg aatcttcctg caagcatttt gaacagttat ccagcgattg gacgcgtgat tattggcgct tttttaaatg cgatggttac ggctgacgga aaatataaaa agcgtgtttt gtttgtcttg gatgaagttg atcttcttgg ttatatgaat attttagagg aagcacgcga ccgtgggcgt aaatacggca catccttgat gctcttttat caatcctctg gtcagttggt caatcacttt ggagaagcgg gggcgcgttc atggtttgaa agttgctcat ttgtcagtta cgccgccatt aaagatctcc aaacagccaa agatatttca gagcgctgtg gtcaaatgac ggttgaagta accggaacaa acaaatcgag aggtttgtct ttgggaaaga gctcttacaa cgtcaattat caacaaagag ccttgatttt accccatgaa attattcagg aaatgcgcca agatgaacaa atcatcctta tgcaaggaca acccccttta cgatgtggtc gagcgattta ctttagaaga aaagaaatgt tagccgctgc taacaaaaac cgttttgctc cacaagcaaa gaaaaattaa
Details for BaquA.18398.a.B2.GE36072
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 56kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Validated AA Sequence
MNLKEAAKIF PANGQIVVGE RYRVDQDGVC NIPFAPGNKT TWGKGGTAPL LTFNLDFGST HMIFFAGSGG YKTTSTVVPT CLTYPGPIVC LDPSTEIAPM VRFARKKMGN RNVIVLDPNS LLTKNFNVLD WLLDDSVPRT QREANIVGFS KLLLTDKKSE NSSAEYFSTQ AHNLLTALLA HVIFSDEYED SERNLKTLRA ILSQSETAVV NQLRMIQETT PSPFIREMVG IFTEMADQXF SGVYTTASKD TQWLSLSNYA DLVCGNDFSS SDIANGKTDV FLNLPASILN SYPAIGRVII GAFLNAMVTA DGKYKKRVLF VLDEVDLLGY MNILEEARDR GRKYGTSLML FYQSSGQLVN HFGEAGARSW FESCSFVSYA AIKDLQTAKD ISERCGQMTV EVTGTNKSRG LSLGKSSYNV NYQQRALILP HEIIQEMRQD EQIILMQGQP PLRCGRAIYF RRKEMLAAAN KN
Validated NT Sequence
cggtttttgt tagcagcggc taacatttct tttcttctaa agtaaatcgc tcgaccacat cgtaaagggg gttgtccttg cataaggatg atttgttcat cttggcgcat ttcctgaata atttcatggg gtaaaatcaa ggctctttgt tgataattga cgttgtaaga gctctttccc aaagacaaac ctctcgattt gtttgttccg gttacttcaa ccgtcatttg accacagcgc tctgaaatat ctttggctgt ttggagatct ttaatggcgg cgtaactgac aaatgagcaa ctttcaaacc atgaacgcgc ccccgcttct ccaaagtgat tgaccaactg accagaggat tgataaaaga gcatcaagga tgtgccgtat ttacgcccac ggtcgcgtgc ttcctctaaa atattcatat aaccaagaag atcaacttca tccaagacaa acaaaacacg ctttttatat tttccgtcag ccgtaaccat cgcatttaaa aaagcgccaa taatcacgcg tccaatcgct ggataactgt tcaaaatgct tgcaggaaga ttcagaaaaa catctgtctt tccatttgca atatccgaag acgaaaaatc attgccacaa acaagatcag cgtaattgga caaagaaagc cactgggtat cttttgatgc cgttgtatag acccctgaaa aggnttggtc tgccatttcg gtgaaaatac caaccatttc acgaataaaa ggagaaggtg ttgtttcttg aatcatgcgc aattgattca caacagccgt ttctgactga gaaaggatcg cgcgcaatgt ttttaaattg cgctcactat cttcatattc atcagagaag atgacatgtg caagaagcgc agttaaaaga ttatgtgctt gtgtcgaaaa atattctgcc gaagaattct ctgatttttt atctgtaaga agcagtttgg aaaaaccgac aatattagct tcacgttgtg tgcgcggtac gctatcgtct aaaagccaat caagtacatt aaaatttttt gttaagaggg aattgggatc gagaacaata acatttctat tgcccatttt tttccgcgca aacctaacca ttggcgcaat ttctgttgaa ggatcaaggc aaacaatagg tcctggatat gttaaacagg tcggaacaac tgtacttgtt gttttatacc caccagaacc ggcaaaaaag atcatgtggg ttgaaccaaa atcaagatta aaggtaagca aaggggctgt tccccctttt ccccatgttg ttttattgcc aggcgcaaag ggaatattgc acacaccgtc ttggtcaaca cggtatcttt cgccaacaac aatttgacca ttggcgggaa aaatttttgc tgcttctttt aaattcatcc atgaggcatc cccaaaaatt ccttttttcg c
Expected Protein Sequence
MAHHHHHHMK RAKKGIFGDA SWMNLKEAAK IFPANGQIVV GERYRVDQDG VCNIPFAPGN KTTWGKGGTA PLLTFNLDFG STHMIFFAGS GGYKTTSTVV PTCLTYPGPI VCLDPSTEIA PMVRFARKKM GNRNVIVLDP NSLLTKNFNV LDWLLDDSVP RTQREANIVG FSKLLLTDKK SENSSAEYFS TQAHNLLTAL LAHVIFSDEY EDSERNLKTL RAILSQSETA VVNQLRMIQE TTPSPFIREM VGIFTEMADQ TFSGVYTTAS KDTQWLSLSN YADLVCGNDF SSSDIANGKT DVFLNLPASI LNSYPAIGRV IIGAFLNAMV TADGKYKKRV LFVLDEVDLL GYMNILEEAR DRGRKYGTSL MLFYQSSGQL VNHFGEAGAR SWFESCSFVS YAAIKDLQTA KDISERCGQM TVEVTGTNKS RGLSLGKSSY NVNYQQRALI LPHEIIQEMR QDEQIILMQG QPPLRCGRAI YFRRKEMLAA ANKNRFAPQA KKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc gtgcgaaaaa aggaattttt ggggatgcct catggatgaa tttaaaagaa gcagcaaaaa tttttcccgc caatggtcaa attgttgttg gcgaaagata ccgtgttgac caagacggtg tgtgcaatat tccctttgcg cctggcaata aaacaacatg gggaaaaggg ggaacagccc ctttgcttac ctttaatctt gattttggtt caacccacat gatctttttt gccggttctg gtgggtataa aacaacaagt acagttgttc cgacctgttt aacatatcca ggacctattg tttgccttga tccttcaaca gaaattgcgc caatggttag gtttgcgcgg aaaaaaatgg gcaatagaaa tgttattgtt ctcgatccca attccctctt aacaaaaaat tttaatgtac ttgattggct tttagacgat agcgtaccgc gcacacaacg tgaagctaat attgtcggtt tttccaaact gcttcttacg gataaaaaat cagagaattc ttcggcagaa tatttttcga cacaagcaca taatctttta actgcgcttc ttgcacatgt catcttctct gatgaatatg aagatagtga gcgcaattta aaaacattgc gcgcgatcct ttctcagtca gaaacggctg ttgtgaatca attgcgcatg attcaagaaa caacaccttc tccttttatt cgtgaaatgg ttggtatttt caccgaaatg gcagaccaaa ccttttcagg ggtctataca acggcatcaa aagataccca gtggctttct ttgtccaatt acgctgatct tgtttgtggc aatgattttt cgtcttcgga tattgcaaat ggaaagacag atgtttttct gaatcttcct gcaagcattt tgaacagtta tccagcgatt ggacgcgtga ttattggcgc ttttttaaat gcgatggtta cggctgacgg aaaatataaa aagcgtgttt tgtttgtctt ggatgaagtt gatcttcttg gttatatgaa tattttagag gaagcacgcg accgtgggcg taaatacggc acatccttga tgctctttta tcaatcctct ggtcagttgg tcaatcactt tggagaagcg ggggcgcgtt catggtttga aagttgctca tttgtcagtt acgccgccat taaagatctc caaacagcca aagatatttc agagcgctgt ggtcaaatga cggttgaagt aaccggaaca aacaaatcga gaggtttgtc tttgggaaag agctcttaca acgtcaatta tcaacaaaga gccttgattt taccccatga aattattcag gaaatgcgcc aagatgaaca aatcatcctt atgcaaggac aacccccttt acgatgtggt cgagcgattt actttagaag aaaagaaatg ttagccgctg ctaacaaaaa ccgttttgct ccacaagcaa agaaaaattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BaquA.18398.a.B2.PW37230
PURIFICATION DATe: 1/8/2013
CONCENTRATION: 21.8mg/ml
OBSERVED MW: 57kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MNLKEAAKIF PANGQIVVGE RYRVDQDGVC NIPFAPGNKT TWGKGGTAPL LTFNLDFGST HMIFFAGSGG YKTTSTVVPT CLTYPGPIVC LDPSTEIAPM VRFARKKMGN RNVIVLDPNS LLTKNFNVLD WLLDDSVPRT QREANIVGFS KLLLTDKKSE NSSAEYFSTQ AHNLLTALLA HVIFSDEYED SERNLKTLRA ILSQSETAVV NQLRMIQETT PSPFIREMVG IFTEMADQXF SGVYTTASKD TQWLSLSNYA DLVCGNDFSS SDIANGKTDV FLNLPASILN SYPAIGRVII GAFLNAMVTA DGKYKKRVLF VLDEVDLLGY MNILEEARDR GRKYGTSLML FYQSSGQLVN HFGEAGARSW FESCSFVSYA AIKDLQTAKD ISERCGQMTV EVTGTNKSRG LSLGKSSYNV NYQQRALILP HEIIQEMRQD EQIILMQGQP PLRCGRAIYF RRKEMLAAAN KN
Validated NT Sequence
cggtttttgt tagcagcggc taacatttct tttcttctaa agtaaatcgc tcgaccacat cgtaaagggg gttgtccttg cataaggatg atttgttcat cttggcgcat ttcctgaata atttcatggg gtaaaatcaa ggctctttgt tgataattga cgttgtaaga gctctttccc aaagacaaac ctctcgattt gtttgttccg gttacttcaa ccgtcatttg accacagcgc tctgaaatat ctttggctgt ttggagatct ttaatggcgg cgtaactgac aaatgagcaa ctttcaaacc atgaacgcgc ccccgcttct ccaaagtgat tgaccaactg accagaggat tgataaaaga gcatcaagga tgtgccgtat ttacgcccac ggtcgcgtgc ttcctctaaa atattcatat aaccaagaag atcaacttca tccaagacaa acaaaacacg ctttttatat tttccgtcag ccgtaaccat cgcatttaaa aaagcgccaa taatcacgcg tccaatcgct ggataactgt tcaaaatgct tgcaggaaga ttcagaaaaa catctgtctt tccatttgca atatccgaag acgaaaaatc attgccacaa acaagatcag cgtaattgga caaagaaagc cactgggtat cttttgatgc cgttgtatag acccctgaaa aggnttggtc tgccatttcg gtgaaaatac caaccatttc acgaataaaa ggagaaggtg ttgtttcttg aatcatgcgc aattgattca caacagccgt ttctgactga gaaaggatcg cgcgcaatgt ttttaaattg cgctcactat cttcatattc atcagagaag atgacatgtg caagaagcgc agttaaaaga ttatgtgctt gtgtcgaaaa atattctgcc gaagaattct ctgatttttt atctgtaaga agcagtttgg aaaaaccgac aatattagct tcacgttgtg tgcgcggtac gctatcgtct aaaagccaat caagtacatt aaaatttttt gttaagaggg aattgggatc gagaacaata acatttctat tgcccatttt tttccgcgca aacctaacca ttggcgcaat ttctgttgaa ggatcaaggc aaacaatagg tcctggatat gttaaacagg tcggaacaac tgtacttgtt gttttatacc caccagaacc ggcaaaaaag atcatgtggg ttgaaccaaa atcaagatta aaggtaagca aaggggctgt tccccctttt ccccatgttg ttttattgcc aggcgcaaag ggaatattgc acacaccgtc ttggtcaaca cggtatcttt cgccaacaac aatttgacca ttggcgggaa aaatttttgc tgcttctttt aaattcatcc atgaggcatc cccaaaaatt ccttttttcg c
Expressed Protein Sequence
MAHHHHHHMK RAKKGIFGDA SWMNLKEAAK IFPANGQIVV GERYRVDQDG VCNIPFAPGN KTTWGKGGTA PLLTFNLDFG STHMIFFAGS GGYKTTSTVV PTCLTYPGPI VCLDPSTEIA PMVRFARKKM GNRNVIVLDP NSLLTKNFNV LDWLLDDSVP RTQREANIVG FSKLLLTDKK SENSSAEYFS TQAHNLLTAL LAHVIFSDEY EDSERNLKTL RAILSQSETA VVNQLRMIQE TTPSPFIREM VGIFTEMADQ TFSGVYTTAS KDTQWLSLSN YADLVCGNDF SSSDIANGKT DVFLNLPASI LNSYPAIGRV IIGAFLNAMV TADGKYKKRV LFVLDEVDLL GYMNILEEAR DRGRKYGTSL MLFYQSSGQL VNHFGEAGAR SWFESCSFVS YAAIKDLQTA KDISERCGQM TVEVTGTNKS RGLSLGKSSY NVNYQQRALI LPHEIIQEMR QDEQIILMQG QPPLRCGRAI YFRRKEMLAA ANKNRFAPQA KKN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGC GTGCGAAAAA AGGAATTTTT GGGGATGCCT CATGGATGAA TTTAAAAGAA GCAGCAAAAA TTTTTCCCGC CAATGGTCAA ATTGTTGTTG GCGAAAGATA CCGTGTTGAC CAAGACGGTG TGTGCAATAT TCCCTTTGCG CCTGGCAATA AAACAACATG GGGAAAAGGG GGAACAGCCC CTTTGCTTAC CTTTAATCTT GATTTTGGTT CAACCCACAT GATCTTTTTT GCCGGTTCTG GTGGGTATAA AACAACAAGT ACAGTTGTTC CGACCTGTTT AACATATCCA GGACCTATTG TTTGCCTTGA TCCTTCAACA GAAATTGCGC CAATGGTTAG GTTTGCGCGG AAAAAAATGG GCAATAGAAA TGTTATTGTT CTCGATCCCA ATTCCCTCTT AACAAAAAAT TTTAATGTAC TTGATTGGCT TTTAGACGAT AGCGTACCGC GCACACAACG TGAAGCTAAT ATTGTCGGTT TTTCCAAACT GCTTCTTACG GATAAAAAAT CAGAGAATTC TTCGGCAGAA TATTTTTCGA CACAAGCACA TAATCTTTTA ACTGCGCTTC TTGCACATGT CATCTTCTCT GATGAATATG AAGATAGTGA GCGCAATTTA AAAACATTGC GCGCGATCCT TTCTCAGTCA GAAACGGCTG TTGTGAATCA ATTGCGCATG ATTCAAGAAA CAACACCTTC TCCTTTTATT CGTGAAATGG TTGGTATTTT CACCGAAATG GCAGACCAAA CCTTTTCAGG GGTCTATACA ACGGCATCAA AAGATACCCA GTGGCTTTCT TTGTCCAATT ACGCTGATCT TGTTTGTGGC AATGATTTTT CGTCTTCGGA TATTGCAAAT GGAAAGACAG ATGTTTTTCT GAATCTTCCT GCAAGCATTT TGAACAGTTA TCCAGCGATT GGACGCGTGA TTATTGGCGC TTTTTTAAAT GCGATGGTTA CGGCTGACGG AAAATATAAA AAGCGTGTTT TGTTTGTCTT GGATGAAGTT GATCTTCTTG GTTATATGAA TATTTTAGAG GAAGCACGCG ACCGTGGGCG TAAATACGGC ACATCCTTGA TGCTCTTTTA TCAATCCTCT GGTCAGTTGG TCAATCACTT TGGAGAAGCG GGGGCGCGTT CATGGTTTGA AAGTTGCTCA TTTGTCAGTT ACGCCGCCAT TAAAGATCTC CAAACAGCCA AAGATATTTC AGAGCGCTGT GGTCAAATGA CGGTTGAAGT AACCGGAACA AACAAATCGA GAGGTTTGTC TTTGGGAAAG AGCTCTTACA ACGTCAATTA TCAACAAAGA GCCTTGATTT TACCCCATGA AATTATTCAG GAAATGCGCC AAGATGAACA AATCATCCTT ATGCAAGGAC AACCCCCTTT ACGATGTGGT CGAGCGATTT ACTTTAGAAG AAAAGAAATG TTAGCCGCTG CTAACAAAAA CCGTTTTGCT CCACAAGCAA AGAAAAATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT