BaquA.17838.a

Putative histidine kinase of the PhyR response regulator (putative uncharacterized protein)

CENTER ID: BaquA.17838.a
ORGANISM: Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.17838.a.B2.GE37172 domain1 54 204
BaquA.17838.a.B3.GE37173 includes HWE_HK HATPase_c 225 485
BaquA.17838.a.B31.GE39455 includes HWE_HK HATPase_c 225 485
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.17838.a.A4.PS37817 HWE_HK HATPase_c 280 473

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283165.4.peg.1188
RefSeq: YP_032648.1
UniProt: A0A0H3LWH6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIFRFANGN FMTTKTNLLP KISSSDSTIS RWRWVAIIVS LVMALLASTF IILLSNAVDR EVAQLNTSSE LREQADLVLI SLIDMAVADR SYAKTRKAED RARFENAVRS LDDILKYTEL IVYAHPQWYE WFNAFKKEVE VRKAFMNDHM LALDALPDQS SQPSFSSEIP KIQVARLAQL MTSFINGDDV VRQKQRENIA LMRAALTLAA IVSVVSTFVS AYFVINRFHR DVRSLKMYQL LLHSENIALE ARVKERTQEL EKARNHAEKE RQRVEMLLQD ASHRIGNSLG TVSSLLGVQL NRSTNEEVRS VLGAARDRIQ TISTAHRRLR LSDDMETTLL AEFLSSVVHD VELAVPFELR ENITIHTSFK ECRLSSRDAT TLGIILGELL TNSLKHAFPD KRSGHIYISF GPQEDGQLML IVEDNGVGMK HQTSEQKAGL GRVVVEQLCM QFGEKPIFET SALGGTKILI PLPKLSTNDS KEASA
NT Sequence
atgacgattt tcaggttcgc caatggaaat tttatgacaa caaagacaaa tttgcttcct aaaatatcct cttctgattc aacaatatca cgttggcgtt gggtcgcgat tattgtttct cttgttatgg cccttttggc ttccaccttt attatactgc tctcaaacgc cgttgatcgc gaagttgcac agttgaatac gagctcagaa ttgcgcgaac aggctgattt agtgctcatt agtttgatcg atatggctgt cgctgatcgt agctatgcaa aaacccgcaa ggcggaagac agagcgcgct ttgaaaatgc tgttcgtagt cttgatgata ttctcaagta tacggaactt attgtttacg cacatccgca atggtatgaa tggtttaatg cttttaaaaa agaggtcgaa gtacgcaaag cttttatgaa tgaccatatg cttgctcttg atgcacttcc tgatcaatct tcccaaccct ctttctcctc tgaaattcca aagattcaag tggcgcgttt agcccagttg atgacaagct ttattaatgg agatgatgtg gtgcggcaaa aacagcgcga gaacatcgca cttatgcggg ctgctttaac attggcggct attgtttctg tcgtaagtac ttttgtttcg gcttattttg tcattaatcg ttttcaccgt gatgtacgct ccttaaaaat gtatcaattg ttgcttcata gtgagaatat agcccttgaa gcacgtgtga aagaacgcac acaggaattg gaaaaagcac gcaatcatgc cgagaaagaa cggcaacgtg ttgaaatgtt gttgcaagat gccagccatc gcattggcaa ttctcttggt acagtctctt ctttgcttgg tgtacaatta aatcgaagca caaatgaaga agtgcgttct gttcttgggg ctgcacggga ccgtatccag acaatatcca cggcgcatcg ccgtttgcgt ttaagtgatg atatggaaac aactttattg gcagaatttc tcagttctgt ggtgcatgat gttgaattag cagtgccttt tgaattgcgc gaaaatatca ccattcatac ttcctttaaa gaatgtcggt tgtcttcaag agatgcaaca acactcggaa ttattcttgg tgaattgctg acaaattcct taaaacacgc ctttcctgat aagcgctctg gtcatattta tatctcattt ggtcctcagg aagatggaca actgatgctg attgtggaag ataatggtgt gggcatgaag caccaaactt cagaacaaaa agcaggtctt ggtcgggtgg ttgttgaaca gctttgtatg caatttggtg agaaaccaat ttttgaaact tctgcgttgg gtggcacaaa gattttgatt cctttgccga aattaagtac caatgattca aaagaagcat ctgcttga
Details for BaquA.17838.a.B2.GE37172
HARVESTED ON: 8/13/2013
SEQUENCED ON: 12/18/2014
EXPECTED MW: 18kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML SNAVDREVAQ LNTSSELREQ ADLVLISLID MAVADRSYAK TRKAEDRARF ENAVRSLDDI LKYTELIVYA HPQWYEWFNA FKKEVEVRKA FMNDHMLALD ALPDQSSQPS FSSEIPKIQV ARLAQLMTSF INGDDVVRQK QRENIALMRA
Validated NT Sequence
atggctcacc accaccacca ccatatgctc tcaaacgccg ttgatcgcga agttgcacag ttgaatacga gctcagaatt gcgcgaacag gctgatttag tgctcattag tttgatcgat atggctgtcg ctgatcgtag ctatgcaaaa acccgcaagg cggaagacag agcgcgcttt gaaaatgctg ttcgtagtct tgatgatatt ctcaagtata cggaacttat tgtttacgca catccgcaat ggtatgaatg gtttaatgct tttaaaaaag aggtcgaagt acgcaaagct tttatgaatg accatatgct tgctcttgat gcacttcctg atcaatcttc ccaaccctct ttctcctctg aaattccaaa gattcaagtg gcgcgtttag cccagttgat gacaagcttt attaatggag atgatgtggt gcggcaaaaa cagcgcgaga acatcgcact tatgcgggct taagtgagta agat
Expected Protein Sequence
MAHHHHHHLS NAVDREVAQL NTSSELREQA DLVLISLIDM AVADRSYAKT RKAEDRARFE NAVRSLDDIL KYTELIVYAH PQWYEWFNAF KKEVEVRKAF MNDHMLALDA LPDQSSQPSF SSEIPKIQVA RLAQLMTSFI NGDDVVRQKQ RENIALMRA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctctcaa acgccgttga tcgcgaagtt gcacagttga atacgagctc agaattgcgc gaacaggctg atttagtgct cattagtttg atcgatatgg ctgtcgctga tcgtagctat gcaaaaaccc gcaaggcgga agacagagcg cgctttgaaa atgctgttcg tagtcttgat gatattctca agtatacgga acttattgtt tacgcacatc cgcaatggta tgaatggttt aatgctttta aaaaagaggt cgaagtacgc aaagctttta tgaatgacca tatgcttgct cttgatgcac ttcctgatca atcttcccaa ccctctttct cctctgaaat tccaaagatt caagtggcgc gtttagccca gttgatgaca agctttatta atggagatga tgtggtgcgg caaaaacagc gcgagaacat cgcacttatg cgggcttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BaquA.17838.a.B3.GE37173
HARVESTED ON: 8/13/2013
SEQUENCED ON: 12/18/2014
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI NRFHRDVRSL KMYQLLLHSE NIALEARVKE RTQELEKARN HAEKERQRVE MLLQDASHRI GNSLGTVSSL LGVQLNRSTN EEVRSVLGAA RDRIQTISTA HRRLRLSDDM ETTLLAEFLS SVVHDVELAV PFELRENITI HTSFKECRLS SRDATTLGII LGELLTNSLK HAFPDKRSGH IYISFGPQED GQLMLIVEDN GVGMKHQTSE QKAGLGRVVV EQLCMQFGEK PIFETSALGG TKILIPLPKL STNDSKEASA
Validated NT Sequence
atcttactca cttaagcaga tgcttctttt gaatcattgg tacttaattt cggcaaagga atcaaaatct ttgtgccacc caacgcagaa gtttcaaaaa ttggtttctc accaaattgc atacaaagct gttcaacaac cacccgacca agacctgctt tttgttctga agtttggtgc ttcatgccca caccattatc ttccacaatc agcatcagtt gtccatcttc ctgaggacca aatgagatat aaatatgacc agagcgctta tcaggaaagg cgtgttttaa ggaatttgtc agcaattcac caagaataat tccgagtgtt gttgcatctc ttgaagacaa ccgacattct ttaaaggaag tatgaatggt gatattttcg cgcaattcaa aaggcactgc taattcaaca tcatgcacca cagaactgag aaattctgcc aataaagttg tttccatatc atcacttaaa cgcaaacggc gatgcgccgt ggatattgtc tggatacggt cccgtgcagc cccaagaaca gaacgcactt cttcatttgt gcttcgattt aattgtacac caagcaaaga agagactgta ccaagagaat tgccaatgcg atggctggca tcttgcaaca acatttcaac acgttgccgt tctttctcgg catgattgcg tgctttttcc aattcctgtg tgcgttcttt cacacgtgct tcaagggcta tattctcact atgaagcaac aattgataca tttttaagga gcgtacatca cggtgaaaac gattaatcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHIN RFHRDVRSLK MYQLLLHSEN IALEARVKER TQELEKARNH AEKERQRVEM LLQDASHRIG NSLGTVSSLL GVQLNRSTNE EVRSVLGAAR DRIQTISTAH RRLRLSDDME TTLLAEFLSS VVHDVELAVP FELRENITIH TSFKECRLSS RDATTLGIIL GELLTNSLKH AFPDKRSGHI YISFGPQEDG QLMLIVEDNG VGMKHQTSEQ KAGLGRVVVE QLCMQFGEKP IFETSALGGT KILIPLPKLS TNDSKEASA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattaatc gttttcaccg tgatgtacgc tccttaaaaa tgtatcaatt gttgcttcat agtgagaata tagcccttga agcacgtgtg aaagaacgca cacaggaatt ggaaaaagca cgcaatcatg ccgagaaaga acggcaacgt gttgaaatgt tgttgcaaga tgccagccat cgcattggca attctcttgg tacagtctct tctttgcttg gtgtacaatt aaatcgaagc acaaatgaag aagtgcgttc tgttcttggg gctgcacggg accgtatcca gacaatatcc acggcgcatc gccgtttgcg tttaagtgat gatatggaaa caactttatt ggcagaattt ctcagttctg tggtgcatga tgttgaatta gcagtgcctt ttgaattgcg cgaaaatatc accattcata cttcctttaa agaatgtcgg ttgtcttcaa gagatgcaac aacactcgga attattcttg gtgaattgct gacaaattcc ttaaaacacg cctttcctga taagcgctct ggtcatattt atatctcatt tggtcctcag gaagatggac aactgatgct gattgtggaa gataatggtg tgggcatgaa gcaccaaact tcagaacaaa aagcaggtct tggtcgggtg gttgttgaac agctttgtat gcaatttggt gagaaaccaa tttttgaaac ttctgcgttg ggtggcacaa agattttgat tcctttgccg aaattaagta ccaatgattc aaaagaagca tctgcttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BaquA.17838.a.B31.GE39455
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI NRFHRDVRSL KMYQLLLHSE NIALEARVKE RTQELEKARN HAEKERQRVE MLLQDASHRI GNSLGTVSSL LGVQLNRSTN EEVRSVLGAA RDRIQTISTA HRRLRLSDDM ETTLLAEFLS SVVHDVELAV PFELRENITI HTSFKECRLS SRDATTLGII LGELLTNSLK HAFPDKRSGH IYISFGPQED GQLMLIVEDN GVGMKHQTSE QKAGLGRVVV EQLCMQFGEK PIFETSALGG TKILIPLPKL STNDSKEASA
Validated NT Sequence
atcttactca cttaagcaga tgcttctttt gaatcattgg tacttaattt cggcaaagga atcaaaatct ttgtgccacc caacgcagaa gtttcaaaaa ttggtttctc accaaattgc atacaaagct gttcaacaac cacccgacca agacctgctt tttgttctga agtttggtgc ttcatgccca caccattatc ttccacaatc agcatcagtt gtccatcttc ctgaggacca aatgagatat aaatatgacc agagcgctta tcaggaaagg cgtgttttaa ggaatttgtc agcaattcac caagaataat tccgagtgtt gttgcatctc ttgaagacaa ccgacattct ttaaaggaag tatgaatggt gatattttcg cgcaattcaa aaggcactgc taattcaaca tcatgcacca cagaactgag aaattctgcc aataaagttg tttccatatc atcacttaaa cgcaaacggc gatgcgccgt ggatattgtc tggatacggt cccgtgcagc cccaagaaca gaacgcactt cttcatttgt gcttcgattt aattgtacac caagcaaaga agagactgta ccaagagaat tgccaatgcg atggctggca tcttgcaaca acatttcaac acgttgccgt tctttctcgg catgattgcg tgctttttcc aattcctgtg tgcgttcttt cacacgtgct tcaagggcta tattctcact atgaagcaac aattgataca tttttaagga gcgtacatca cggtgaaaac gattaatcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHIN RFHRDVRSLK MYQLLLHSEN IALEARVKER TQELEKARNH AEKERQRVEM LLQDASHRIG NSLGTVSSLL GVQLNRSTNE EVRSVLGAAR DRIQTISTAH RRLRLSDDME TTLLAEFLSS VVHDVELAVP FELRENITIH TSFKECRLSS RDATTLGIIL GELLTNSLKH AFPDKRSGHI YISFGPQEDG QLMLIVEDNG VGMKHQTSEQ KAGLGRVVVE QLCMQFGEKP IFETSALGGT KILIPLPKLS TNDSKEASA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattaatc gttttcaccg tgatgtacgc tccttaaaaa tgtatcaatt gttgcttcat agtgagaata tagcccttga agcacgtgtg aaagaacgca cacaggaatt ggaaaaagca cgcaatcatg ccgagaaaga acggcaacgt gttgaaatgt tgttgcaaga tgccagccat cgcattggca attctcttgg tacagtctct tctttgcttg gtgtacaatt aaatcgaagc acaaatgaag aagtgcgttc tgttcttggg gctgcacggg accgtatcca gacaatatcc acggcgcatc gccgtttgcg tttaagtgat gatatggaaa caactttatt ggcagaattt ctcagttctg tggtgcatga tgttgaatta gcagtgcctt ttgaattgcg cgaaaatatc accattcata cttcctttaa agaatgtcgg ttgtcttcaa gagatgcaac aacactcgga attattcttg gtgaattgct gacaaattcc ttaaaacacg cctttcctga taagcgctct ggtcatattt atatctcatt tggtcctcag gaagatggac aactgatgct gattgtggaa gataatggtg tgggcatgaa gcaccaaact tcagaacaaa aagcaggtct tggtcgggtg gttgttgaac agctttgtat gcaatttggt gagaaaccaa tttttgaaac ttctgcgttg ggtggcacaa agattttgat tcctttgccg aaattaagta ccaatgattc aaaagaagca tctgcttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BaquA.17838.a.A4.PS37817
PURIFICATION DATe: 1/20/2016
CONCENTRATION: 30.15mg/ml
OBSERVED MW: 24kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDASHRIGN SLGTVSSLLG VQLNRSTNEE VRSVLGAARD RIQTISTAHR RLRLSDDMET TLLAEFLSSV VHDVELAVPF ELRENITIHT SFKECRLSSR DATTLGIILG ELLTNSLKHA FPDKRSGHIY ISFGPQEDGQ LMLIVEDNGV GMKHQTSEQK AGLGRVVVEQ LCMQFGEKPI FETSALGGTK ILIPLP
Validated NT Sequence
tattacggca aaggaatcaa aatctttgtg ccacccaacg cagaagtttc aaaaattggt ttctcaccaa attgcataca aagctgttca acaaccaccc gaccaagacc tgctttttgt tctgaagttt ggtgcttcat gcccacacca ttatcttcca caatcagcat cagttgtcca tcttcctgag gaccaaatga gatataaata tgaccagagc gcttatcagg aaaggcgtgt tttaaggaat ttgtcagcaa ttcaccaaga ataattccga gtgttgttgc atctcttgaa gacaaccgac attctttaaa ggaagtatga atggtgatat tttcgcgcaa ttcaaaaggc actgctaatt caacatcatg caccacagaa ctgagaaatt ctgccaataa agttgtttcc atatcatcac ttaaacgcaa acggcgatgc gccgtggata ttgtctggat acggtcccgt gcagccccaa gaacagaacg cacttcttca tttgtgcttc gatttaattg tacaccaagc aaagaagaga ctgtaccaag agaattgcca atgcgatggc tggcatccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SDASHRIGNS LGTVSSLLGV QLNRSTNEEV RSVLGAARDR IQTISTAHRR LRLSDDMETT LLAEFLSSVV HDVELAVPFE LRENITIHTS FKECRLSSRD ATTLGIILGE LLTNSLKHAF PDKRSGHIYI SFGPQEDGQL MLIVEDNGVG MKHQTSEQKA GLGRVVVEQL CMQFGEKPIF ETSALGGTKI LIPLP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGA TGCCAGCCAT CGCATTGGCA ATTCTCTTGG TACAGTCTCT TCTTTGCTTG GTGTACAATT AAATCGAAGC ACAAATGAAG AAGTGCGTTC TGTTCTTGGG GCTGCACGGG ACCGTATCCA GACAATATCC ACGGCGCATC GCCGTTTGCG TTTAAGTGAT GATATGGAAA CAACTTTATT GGCAGAATTT CTCAGTTCTG TGGTGCATGA TGTTGAATTA GCAGTGCCTT TTGAATTGCG CGAAAATATC ACCATTCATA CTTCCTTTAA AGAATGTCGG TTGTCTTCAA GAGATGCAAC AACACTCGGA ATTATTCTTG GTGAATTGCT GACAAATTCC TTAAAACACG CCTTTCCTGA TAAGCGCTCT GGTCATATTT ATATCTCATT TGGTCCTCAG GAAGATGGAC AACTGATGCT GATTGTGGAA GATAATGGTG TGGGCATGAA GCACCAAACT TCAGAACAAA AAGCAGGTCT TGGTCGGGTG GTTGTTGAAC AGCTTTGTAT GCAATTTGGT GAGAAACCAA TTTTTGAAAC TTCTGCGTTG GGTGGCACAA AGATTTTGAT TCCTTTGCCG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA