SSGCID: Target Page

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
BaquA.17833.c.B11.GE36919	VompB:aa269-556 (MV:273-330)	269	556
BaquA.17833.c.B12.GE36920	VompB:aa269-679 (MV:273-330)	269	679
BaquA.17833.c.B14.GE37171	VompB:aa269-880 (MV:273-330)	269	880
BaquA.17833.c.B14.GE37440	VompB:aa269-880 (MV:273-330)	269	880
BaquA.17833.c.B2.GE36660	mature protein, soluble domain	81	925
BaquA.17833.c.B4.GE36662	VompB:aa362-430	362	430
BaquA.17833.c.B5.GE36921	VompB:aa465-556	445	556
BaquA.17833.c.B7.GE37092	VompB:aa680-803	680	803
BaquA.17833.c.B8.GE36838	VompB:aa805-880	805	880
BaquA.17833.c.B9.GE36839	VompB:aa158-453 (MV:273-330)	158	453
BaquA.17833.c.MB3.GE38119	VompB:aa269-361 (MV:273-330)	269	361
BaquA.17833.c.MB9.GE38120	VompB:aa158-453 (MV:273-330)	158	453

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.17833.c.B4.PW37278	VompB:aa362-430		362	430	unavailable
BaquA.17833.c.B5.PW37328	VompB:aa465-556		445	556

RESOURCE	REFERENCE ID
UniProt:	Q64HS8

Details for BaquA.17833.c.B11.GE36919

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS NTYEYGSVAI GXNSNTXVGR GVALGFNSVN NRYADKTYYN PRTGEIDQSS EWTLASALDS LSIGNVEAGT TRQLTGVAAG WEDTDAVNVA QLKALREWAE ESFSGGEITY DEGSKVIRIG SKRSGDKIDI KNSDGQDRVL AGVKDGEVSK GSVDAINGSQ LWKTNQTVAG LNVTLNDLGK NLTNYLGGGA DVVQNVAPTY TIQGKQHRDV GSAFSGVDNI LTDIYSKLGD LPGGGVKDQD ALMWSDTENA FVALHTKDGN KTNSKITYLL DGDISKASTD AINGSQL

Validated NT Sequence

atcttactca cttaaagctg agaaccattt atggcatctg ttgaagcttt actaatgtca ccatccaaaa gataggtaat cttactgttc gttttgtttc cgtcttttgt gtgaagtgca acaaaggcat tctcagtatc actccacatt aaggcatctt gatccttaac gcctccacca ggcaaatccc caagtttact ataaatatct gtaagtatgt tatcaacacc actaaaagca gaaccaacat cacgatgctg tttgccctga atagtgtaag ttggtgcaac attctgaacc acatctgcac caccacctaa atagtttgtg agattcttac ctagatcatt taaagttaca tttagacccg caactgtttg attggttttc caaagttgac tgccatttat tgcgtcaact gaccccttag aaacctcccc gtccttcact ccagccaaaa ctcgatcttg cccatcgcta tttttaatgt ctattttatc tcccgagcgc tttgaaccaa tgcgaataac cttagaacct tcatcatagg taatctcccc ccctgagaag ctttcttctg cccactctct taacgctttt agttgtgcta cattgactgc atcggtgtct tcccagcctg ctgccactcc tgtcaactgt cgggtcgtac ccgcctcaac gttaccaata ctcagtgaat ctaaggcact ggccaaggtc cattcgctgc tttgatcaat ctcacccgtt ctagggttat aataagtctt gtcagcataa cgattattga cagaattaaa acctaaagct acgccacgac caacangtgt attgctattt cnaccaatag caacagatcc atattcataa gtgttcgaca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHSN TYEYGSVAIG RNSNTPVGRG VALGFNSVNN RYADKTYYNP RTGEIDQSSE WTLASALDSL SIGNVEAGTT RQLTGVAAGW EDTDAVNVAQ LKALREWAEE SFSGGEITYD EGSKVIRIGS KRSGDKIDIK NSDGQDRVLA GVKDGEVSKG SVDAINGSQL WKTNQTVAGL NVTLNDLGKN LTNYLGGGAD VVQNVAPTYT IQGKQHRDVG SAFSGVDNIL TDIYSKLGDL PGGGVKDQDA LMWSDTENAF VALHTKDGNK TNSKITYLLD GDISKASTDA INGSQL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgaaca cttatgaata tggatctgtt gctattggtc gaaatagcaa tacacctgtt ggtcgtggcg tagctttagg ttttaattct gtcaataatc gttatgctga caagacttat tataacccta gaacgggtga gattgatcaa agcagcgaat ggaccttggc cagtgcctta gattcactga gtattggtaa cgttgaggcg ggtacgaccc gacagttgac aggagtggca gcaggctggg aagacaccga tgcagtcaat gtagcacaac taaaagcgtt aagagagtgg gcagaagaaa gcttctcagg gggggagatt acctatgatg aaggttctaa ggttattcgc attggttcaa agcgctcggg agataaaata gacattaaaa atagcgatgg gcaagatcga gttttggctg gagtgaagga cggggaggtt tctaaggggt cagttgacgc aataaatggc agtcaacttt ggaaaaccaa tcaaacagtt gcgggtctaa atgtaacttt aaatgatcta ggtaagaatc tcacaaacta tttaggtggt ggtgcagatg tggttcagaa tgttgcacca acttacacta ttcagggcaa acagcatcgt gatgttggtt ctgcttttag tggtgttgat aacatactta cagatattta tagtaaactt ggggatttgc ctggtggagg cgttaaggat caagatgcct taatgtggag tgatactgag aatgcctttg ttgcacttca cacaaaagac ggaaacaaaa cgaacagtaa gattacctat cttttggatg gtgacattag taaagcttca acagatgcca taaatggttc tcagctttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BaquA.17833.c.B12.GE36920

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	37

Validated AA Sequence

MWSDTENAFV ALHTKDGNKT NSKITYLLDG DISKASTDAI NGSQLYMMSN KLAAYFGGGA GYENGQWSDP IFTLHQFNPD GNFVEKNYKT VADAFGGVDT VIKDIYSKLG DLPGGGVKDQ DALMWSETEN AFVALHGLEG KKTNSKLKFL LDGAIEQGS

Validated NT Sequence

actcgaccct tgctcaatgg caccatctaa gagaaacttc agtttgctat tcgtcttctt tccttccaat ccatgaagcg caacaaaagc attctcagtc tcgctccaca ttaaggcatc ttgatcctta acgcctccac caggcaaatc cccaagttta ctataaatat ccttgataac cgtatcaaca ccaccaaaag catccgctac agtcttatag ttcttctcaa caaaattgcc atcaggattg aattggtgaa gcgtaaatat agggtccgac cactgcccat tctcatatcc agcaccgcca ccaaaataag cagcaagctt attgctcatc atataaagct gagaaccatt tatggcatct gttgaagctt tactaatgtc accatccaaa agataggtaa tcttactgtt cgttttgttt ccgtcttttg tgtgaagtgc aacaaaggca ttctcagtat cactccacat taaggcatct tgatccttaa cgcctccacc aggcaaatcc ccaagtttac tataaatatc tgtaagtatg ttatcaacac cactaaaagc agaaccaaca tcacgatgct gtttgccctg aatagtgtaa gttggtgcaa cattctgaac cacatctgca ccaccaccta aatagtttgt gagattctta cctagatcat ttaaagttac atttagaccc gcaactgttt gattggtttt ccaaagttga ctgccattta ttgcgtcaac tgacccctta gaaacctccc cgtccttcac tccagccaaa actcgatctt gcccatcgct atttttaatg tctattttat ctcccgagcg ctttgaacca atgcgaataa ccttagaacc ttcatcatag gtaatctccc cccctgagaa gctttcttct gcccactctc ttaacgcttt tagttgtgct acattgactg catcggtgtc ttcccagcct gctgccactc ctgtcaactg tcgggtcgta cccgcctcaa cgttaccaat actcagtgaa tctaaggcac tggccaaggt ccattcgctg ctttgatcaa tctcacccgt tctagggtta taataagtct tgtcagcata acgattattg acagaattaa aacctaaagc tacgccacga ccaacaggtg tattgctatt tcgaccaata gcaacagatc catattcata agtg

Expected Protein Sequence

MAHHHHHHSN TYEYGSVAIG RNSNTPVGRG VALGFNSVNN RYADKTYYNP RTGEIDQSSE WTLASALDSL SIGNVEAGTT RQLTGVAAGW EDTDAVNVAQ LKALREWAEE SFSGGEITYD EGSKVIRIGS KRSGDKIDIK NSDGQDRVLA GVKDGEVSKG SVDAINGSQL WKTNQTVAGL NVTLNDLGKN LTNYLGGGAD VVQNVAPTYT IQGKQHRDVG SAFSGVDNIL TDIYSKLGDL PGGGVKDQDA LMWSDTENAF VALHTKDGNK TNSKITYLLD GDISKASTDA INGSQLYMMS NKLAAYFGGG AGYENGQWSD PIFTLHQFNP DGNFVEKNYK TVADAFGGVD TVIKDIYSKL GDLPGGGVKD QDALMWSETE NAFVALHGLE GKKTNSKLKF LLDGAIEQGS SEAITGNQL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgaaca cttatgaata tggatctgtt gctattggtc gaaatagcaa tacacctgtt ggtcgtggcg tagctttagg ttttaattct gtcaataatc gttatgctga caagacttat tataacccta gaacgggtga gattgatcaa agcagcgaat ggaccttggc cagtgcctta gattcactga gtattggtaa cgttgaggcg ggtacgaccc gacagttgac aggagtggca gcaggctggg aagacaccga tgcagtcaat gtagcacaac taaaagcgtt aagagagtgg gcagaagaaa gcttctcagg gggggagatt acctatgatg aaggttctaa ggttattcgc attggttcaa agcgctcggg agataaaata gacattaaaa atagcgatgg gcaagatcga gttttggctg gagtgaagga cggggaggtt tctaaggggt cagttgacgc aataaatggc agtcaacttt ggaaaaccaa tcaaacagtt gcgggtctaa atgtaacttt aaatgatcta ggtaagaatc tcacaaacta tttaggtggt ggtgcagatg tggttcagaa tgttgcacca acttacacta ttcagggcaa acagcatcgt gatgttggtt ctgcttttag tggtgttgat aacatactta cagatattta tagtaaactt ggggatttgc ctggtggagg cgttaaggat caagatgcct taatgtggag tgatactgag aatgcctttg ttgcacttca cacaaaagac ggaaacaaaa cgaacagtaa gattacctat cttttggatg gtgacattag taaagcttca acagatgcca taaatggttc tcagctttat atgatgagca ataagcttgc tgcttatttt ggtggcggtg ctggatatga gaatgggcag tggtcggacc ctatatttac gcttcaccaa ttcaatcctg atggcaattt tgttgagaag aactataaga ctgtagcgga tgcttttggt ggtgttgata cggttatcaa ggatatttat agtaaacttg gggatttgcc tggtggaggc gttaaggatc aagatgcctt aatgtggagc gagactgaga atgcttttgt tgcgcttcat ggattggaag gaaagaagac gaatagcaaa ctgaagtttc tcttagatgg tgccattgag caagggtcga gtgaagcgat tacgggtaat cagctttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BaquA.17833.c.B14.GE37171

HARVESTED ON:	8/13/2013
SEQUENCED ON:	8/15/2013
EXPECTED MW:	66kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	50

Validated AA Sequence

MAHHHHHHMS NTYEYGSVAI GRNSNTPVGR GVALGFNSVN NRYADKTYYN PRTGEIDQSS EWTLASALDS LSIGNVEAGT TRQLTGVAAG WEDTDAVNVA QLKALREWAE ESFSGGEITY DEGSKVIRIG SKRSGDKIDI KNSDGQDRVL AGVKDGEVSK GSVDAINGSQ LWKTNQTVAG LNVTLNDLGK NLTNYLGGGA DVVQNVAPTY TIQGKQHRDV GSAFSGVDNI LTDIYSKLGD LPGGGVKDQD ALMWSDTENA FVALHTKDGN KTNSKITYLL DGDISKASTD AINGSQXYMM SNXLAAYFXX CXI

Validated NT Sequence

atcttactca cttacaactg accaccattc accgcttctt tcgaaccctt ctctatcttc ccatccgcaa cattatctat caccacaggt tcaccagcat taccaccttg taaactgatt ttattcgttt tattcccatg ctcatctcgg tcatagctaa cagcaccatc aacaacctga ccaatccctt gctcaatagc atcaaccttt tgttcaacac cagagagacg ctcattggtt tcccaaagct gtccaccatt gactgcatct ttcgaacctt gcgcaatatt accctcagct acttccgtaa tcttaccagg ttgaccctta tggctcgcat catacccccc cttcccttca ttccaatgta acacatcaga atcagaagac ggaggtaccg gagatgcccc ttgttcaaca gccttcaaac gatcattgag attagacaaa ctgctatcaa caccaccaaa agcatccgat acagtcttat agctcttttc aacaacacta ccatcaggat tcaattggtg aagcataaat gtagggtcca accactgccc attctcatat ctagcaccgc caccaaaata agcagcaagc ttattgctca tcatataaag ctgattaccc gtaatcgctt cactcgaccc ttgctcaatg gcaccatcta agagaaactt cagtttgcta ttcgtcttct ttccttccaa tccatgaagc gcaacaaaag cattctcagt ctcgctccac attaaggcat cttgatcctt aacgcctcca ccaggcaaat ccccaagttt actataaata tccttgataa ccgtatcaac accaccaaaa gcatccgcta cagtcttata gttcttctca acaaaattgc catcnnnntg aattggtgaa gcgtaaatat agggtccnac cactgcccat tctcatatcc ngcaccnnnc aaaataagca gcaagnttat tgctcatcat ataaanctga ganccattta tggcatctgt tgaagcttta ctaatgtcac catccaaaag ataggtaatc ttactgttcg ttttgtttcc gtcttttgtg tgaagtgcaa caaaggcatt ctcagtatca ctccacatta aggcatcttg atccttaacg cctccaccag gcaaatcccc aagtttacta taaatatctg taagtatgtt atcaacacca ctaaaagcag aaccaacatc acgatgctgt ttgccctgaa tagtgtaagt tggtgcaaca ttctgaacca catctgcacc accacctaaa tagtttgtga gattcttacc tagatcattt aaagttacat ttagacccgc aactgtttga ttggttttcc aaagttgact gccatttatt gcgtcaactg accccttaga aacctccccg tccttcactc cagccaaaac tcgatcttgc ccatcgctat ttttaatgtc tattttatct cccgagcgct ttgaaccaat gcgaataacc ttagaacctt catcataggt aatctccccc cctgagaagc tttcttctgc ccactctctt aacgctttta gttgtgctac attgactgca tcggtgtctt cccagcctgc tgccactcct gtcaactgtc gggtcgtacc cgcctcaacg ttaccaatac tcagtgaatc taaggcactg gccaaggtcc attcgctgct ttgatcaatc tcacccgttc tagggttata ataagtcttg tcagcataac gattattgac agaattaaaa cctaaagcta cgccacgacc aacaggtgta ttgctatttc gaccaatagc aacagatcca tattcataag tgttcgacat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHSN TYEYGSVAIG RNSNTPVGRG VALGFNSVNN RYADKTYYNP RTGEIDQSSE WTLASALDSL SIGNVEAGTT RQLTGVAAGW EDTDAVNVAQ LKALREWAEE SFSGGEITYD EGSKVIRIGS KRSGDKIDIK NSDGQDRVLA GVKDGEVSKG SVDAINGSQL WKTNQTVAGL NVTLNDLGKN LTNYLGGGAD VVQNVAPTYT IQGKQHRDVG SAFSGVDNIL TDIYSKLGDL PGGGVKDQDA LMWSDTENAF VALHTKDGNK TNSKITYLLD GDISKASTDA INGSQLYMMS NKLAAYFGGG AGYENGQWSD PIFTLHQFNP DGNFVEKNYK TVADAFGGVD TVIKDIYSKL GDLPGGGVKD QDALMWSETE NAFVALHGLE GKKTNSKLKF LLDGAIEQGS SEAITGNQLY MMSNKLAAYF GGGARYENGQ WLDPTFMLHQ LNPDGSVVEK SYKTVSDAFG GVDSSLSNLN DRLKAVEQGA SPVPPSSDSD VLHWNEGKGG YDASHKGQPG KITEVAEGNI AQGSKDAVNG GQLWETNERL SGVEQKVDAI EQGIGQVVDG AVSYDRDEHG NKTNKISLQG GNAGEPVVID NVADGKIEKG SKEAVNGGQL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgaaca cttatgaata tggatctgtt gctattggtc gaaatagcaa tacacctgtt ggtcgtggcg tagctttagg ttttaattct gtcaataatc gttatgctga caagacttat tataacccta gaacgggtga gattgatcaa agcagcgaat ggaccttggc cagtgcctta gattcactga gtattggtaa cgttgaggcg ggtacgaccc gacagttgac aggagtggca gcaggctggg aagacaccga tgcagtcaat gtagcacaac taaaagcgtt aagagagtgg gcagaagaaa gcttctcagg gggggagatt acctatgatg aaggttctaa ggttattcgc attggttcaa agcgctcggg agataaaata gacattaaaa atagcgatgg gcaagatcga gttttggctg gagtgaagga cggggaggtt tctaaggggt cagttgacgc aataaatggc agtcaacttt ggaaaaccaa tcaaacagtt gcgggtctaa atgtaacttt aaatgatcta ggtaagaatc tcacaaacta tttaggtggt ggtgcagatg tggttcagaa tgttgcacca acttacacta ttcagggcaa acagcatcgt gatgttggtt ctgcttttag tggtgttgat aacatactta cagatattta tagtaaactt ggggatttgc ctggtggagg cgttaaggat caagatgcct taatgtggag tgatactgag aatgcctttg ttgcacttca cacaaaagac ggaaacaaaa cgaacagtaa gattacctat cttttggatg gtgacattag taaagcttca acagatgcca taaatggttc tcagctttat atgatgagca ataagcttgc tgcttatttt ggtggcggtg ctggatatga gaatgggcag tggtcggacc ctatatttac gcttcaccaa ttcaatcctg atggcaattt tgttgagaag aactataaga ctgtagcgga tgcttttggt ggtgttgata cggttatcaa ggatatttat agtaaacttg gggatttgcc tggtggaggc gttaaggatc aagatgcctt aatgtggagc gagactgaga atgcttttgt tgcgcttcat ggattggaag gaaagaagac gaatagcaaa ctgaagtttc tcttagatgg tgccattgag caagggtcga gtgaagcgat tacgggtaat cagctttata tgatgagcaa taagcttgct gcttattttg gtggcggtgc tagatatgag aatgggcagt ggttggaccc tacatttatg cttcaccaat tgaatcctga tggtagtgtt gttgaaaaga gctataagac tgtatcggat gcttttggtg gtgttgatag cagtttgtct aatctcaatg atcgtttgaa ggctgttgaa caaggggcat ctccggtacc tccgtcttct gattctgatg tgttacattg gaatgaaggg aagggggggt atgatgcgag ccataagggt caacctggta agattacgga agtagctgag ggtaatattg cgcaaggttc gaaagatgca gtcaatggtg gacagctttg ggaaaccaat gagcgtctct ctggtgttga acaaaaggtt gatgctattg agcaagggat tggtcaggtt gttgatggtg ctgttagcta tgaccgagat gagcatggga ataaaacgaa taaaatcagt ttacaaggtg gtaatgctgg tgaacctgtg gtgatagata atgttgcgga tgggaagata gagaagggtt cgaaagaagc ggtgaatggt ggtcagttgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BaquA.17833.c.B14.GE37440

HARVESTED ON:	10/31/2013
SEQUENCED ON:	11/5/2013
EXPECTED MW:	66kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	50

Validated AA Sequence

MAHHHHHHMS NTYEYGSVAI GRNSNTPVGR GVALGFNSVN NRYADKTYYN PRTGEIDQSS EWTLASALDS LSIGNVEAGT TRQLTGVAAG WEDTDAVNVA QLKALREWAE ESFSGGEITY DEGSKVIRIG SKRSGDKIDI KNSDGQDRVL AGVKDGEVSK GSVDAINGSQ LWKTNQTVAG LNVTLNDLGK NLTNYLGGGA DVVQNVAPTY TIQGKQHRDV GSAFSGVDNI LTDIYSKLGD LPGGGVKDQD ALMWSDTENA FVALHTKDGN KTNSKITYLL DGDISKASTD AINGSQXYMM SNXLAAYFXX CXI

Validated NT Sequence

atcttactca cttacaactg accaccattc accgcttctt tcgaaccctt ctctatcttc ccatccgcaa cattatctat caccacaggt tcaccagcat taccaccttg taaactgatt ttattcgttt tattcccatg ctcatctcgg tcatagctaa cagcaccatc aacaacctga ccaatccctt gctcaatagc atcaaccttt tgttcaacac cagagagacg ctcattggtt tcccaaagct gtccaccatt gactgcatct ttcgaacctt gcgcaatatt accctcagct acttccgtaa tcttaccagg ttgaccctta tggctcgcat catacccccc cttcccttca ttccaatgta acacatcaga atcagaagac ggaggtaccg gagatgcccc ttgttcaaca gccttcaaac gatcattgag attagacaaa ctgctatcaa caccaccaaa agcatccgat acagtcttat agctcttttc aacaacacta ccatcaggat tcaattggtg aagcataaat gtagggtcca accactgccc attctcatat ctagcaccgc caccaaaata agcagcaagc ttattgctca tcatataaag ctgattaccc gtaatcgctt cactcgaccc ttgctcaatg gcaccatcta agagaaactt cagtttgcta ttcgtcttct ttccttccaa tccatgaagc gcaacaaaag cattctcagt ctcgctccac attaaggcat cttgatcctt aacgcctcca ccaggcaaat ccccaagttt actataaata tccttgataa ccgtatcaac accaccaaaa gcatccgcta cagtcttata gttcttctca acaaaattgc catcnnnntg aattggtgaa gcgtaaatat agggtccnac cactgcccat tctcatatcc ngcaccnnnc aaaataagca gcaagnttat tgctcatcat ataaanctga ganccattta tggcatctgt tgaagcttta ctaatgtcac catccaaaag ataggtaatc ttactgttcg ttttgtttcc gtcttttgtg tgaagtgcaa caaaggcatt ctcagtatca ctccacatta aggcatcttg atccttaacg cctccaccag gcaaatcccc aagtttacta taaatatctg taagtatgtt atcaacacca ctaaaagcag aaccaacatc acgatgctgt ttgccctgaa tagtgtaagt tggtgcaaca ttctgaacca catctgcacc accacctaaa tagtttgtga gattcttacc tagatcattt aaagttacat ttagacccgc aactgtttga ttggttttcc aaagttgact gccatttatt gcgtcaactg accccttaga aacctccccg tccttcactc cagccaaaac tcgatcttgc ccatcgctat ttttaatgtc tattttatct cccgagcgct ttgaaccaat gcgaataacc ttagaacctt catcataggt aatctccccc cctgagaagc tttcttctgc ccactctctt aacgctttta gttgtgctac attgactgca tcggtgtctt cccagcctgc tgccactcct gtcaactgtc gggtcgtacc cgcctcaacg ttaccaatac tcagtgaatc taaggcactg gccaaggtcc attcgctgct ttgatcaatc tcacccgttc tagggttata ataagtcttg tcagcataac gattattgac agaattaaaa cctaaagcta cgccacgacc aacaggtgta ttgctatttc gaccaatagc aacagatcca tattcataag tgttcgacat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHSN TYEYGSVAIG RNSNTPVGRG VALGFNSVNN RYADKTYYNP RTGEIDQSSE WTLASALDSL SIGNVEAGTT RQLTGVAAGW EDTDAVNVAQ LKALREWAEE SFSGGEITYD EGSKVIRIGS KRSGDKIDIK NSDGQDRVLA GVKDGEVSKG SVDAINGSQL WKTNQTVAGL NVTLNDLGKN LTNYLGGGAD VVQNVAPTYT IQGKQHRDVG SAFSGVDNIL TDIYSKLGDL PGGGVKDQDA LMWSDTENAF VALHTKDGNK TNSKITYLLD GDISKASTDA INGSQLYMMS NKLAAYFGGG AGYENGQWSD PIFTLHQFNP DGNFVEKNYK TVADAFGGVD TVIKDIYSKL GDLPGGGVKD QDALMWSETE NAFVALHGLE GKKTNSKLKF LLDGAIEQGS SEAITGNQLY MMSNKLAAYF GGGARYENGQ WLDPTFMLHQ LNPDGSVVEK SYKTVSDAFG GVDSSLSNLN DRLKAVEQGA SPVPPSSDSD VLHWNEGKGG YDASHKGQPG KITEVAEGNI AQGSKDAVNG GQLWETNERL SGVEQKVDAI EQGIGQVVDG AVSYDRDEHG NKTNKISLQG GNAGEPVVID NVADGKIEKG SKEAVNGGQL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgaaca cttatgaata tggatctgtt gctattggtc gaaatagcaa tacacctgtt ggtcgtggcg tagctttagg ttttaattct gtcaataatc gttatgctga caagacttat tataacccta gaacgggtga gattgatcaa agcagcgaat ggaccttggc cagtgcctta gattcactga gtattggtaa cgttgaggcg ggtacgaccc gacagttgac aggagtggca gcaggctggg aagacaccga tgcagtcaat gtagcacaac taaaagcgtt aagagagtgg gcagaagaaa gcttctcagg gggggagatt acctatgatg aaggttctaa ggttattcgc attggttcaa agcgctcggg agataaaata gacattaaaa atagcgatgg gcaagatcga gttttggctg gagtgaagga cggggaggtt tctaaggggt cagttgacgc aataaatggc agtcaacttt ggaaaaccaa tcaaacagtt gcgggtctaa atgtaacttt aaatgatcta ggtaagaatc tcacaaacta tttaggtggt ggtgcagatg tggttcagaa tgttgcacca acttacacta ttcagggcaa acagcatcgt gatgttggtt ctgcttttag tggtgttgat aacatactta cagatattta tagtaaactt ggggatttgc ctggtggagg cgttaaggat caagatgcct taatgtggag tgatactgag aatgcctttg ttgcacttca cacaaaagac ggaaacaaaa cgaacagtaa gattacctat cttttggatg gtgacattag taaagcttca acagatgcca taaatggttc tcagctttat atgatgagca ataagcttgc tgcttatttt ggtggcggtg ctggatatga gaatgggcag tggtcggacc ctatatttac gcttcaccaa ttcaatcctg atggcaattt tgttgagaag aactataaga ctgtagcgga tgcttttggt ggtgttgata cggttatcaa ggatatttat agtaaacttg gggatttgcc tggtggaggc gttaaggatc aagatgcctt aatgtggagc gagactgaga atgcttttgt tgcgcttcat ggattggaag gaaagaagac gaatagcaaa ctgaagtttc tcttagatgg tgccattgag caagggtcga gtgaagcgat tacgggtaat cagctttata tgatgagcaa taagcttgct gcttattttg gtggcggtgc tagatatgag aatgggcagt ggttggaccc tacatttatg cttcaccaat tgaatcctga tggtagtgtt gttgaaaaga gctataagac tgtatcggat gcttttggtg gtgttgatag cagtttgtct aatctcaatg atcgtttgaa ggctgttgaa caaggggcat ctccggtacc tccgtcttct gattctgatg tgttacattg gaatgaaggg aagggggggt atgatgcgag ccataagggt caacctggta agattacgga agtagctgag ggtaatattg cgcaaggttc gaaagatgca gtcaatggtg gacagctttg ggaaaccaat gagcgtctct ctggtgttga acaaaaggtt gatgctattg agcaagggat tggtcaggtt gttgatggtg ctgttagcta tgaccgagat gagcatggga ataaaacgaa taaaatcagt ttacaaggtg gtaatgctgg tgaacctgtg gtgatagata atgttgcgga tgggaagata gagaagggtt cgaaagaagc ggtgaatggt ggtcagttgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BaquA.17833.c.B2.GE36660

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	93kDa
OBSERVED MW:	93kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	89
PERCENT COVERAGE:	39

Validated AA Sequence

MAHHHHHHMT QEVILDRKTK NDRKTKNDRK TICILSLSVF DGNVVLAEDD DDYVCGLDQV SGFNVLDINP VEESLEKEGI SLTIKREDSA LKRLRALSGV FGVQSIRSGS HPYALGYDAT IFDLGETKKF AGQLYNYLDT NSTETSIGEG VYGWGLSLGA DSNTYEYRNI FEGHVIADSW VSIGKGAYGW GLSLGADSNT YEYGSVAIGR NSNTPVGRGV ALGFNSVNNR YADKTYYNPR TGEIDQSSEW TLASALDSLS IGNVXAGTTR QLTGVAAGWE DTDAXNXXQL KALXXWAEXS FSXGRXPMMX XLXXXXIGSX XSGXXXXXXK XXWXXXX

Validated NT Sequence

ctnnnnnnnn ttnnnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgacccag gaagttattt tggatcgcaa gactaagaat gatcgtaaga ctaagaatga tcgtaagact atatgtattc tttctctaag tgtttttgat ggaaatgttg ttttagcaga agatgatgat gattatgttt gtggtttaga tcaggtttct gggtttaatg tgttagatat caacccagtt gaggagagtt tagagaaaga gggtatttca cttacgataa agagagagga tagcgctttg aagaggctaa gagctctctc tggagttttt ggtgttcagt ctattaggag cggttcccat ccctatgcgc taggttatga tgcaacaatc tttgatctag gtgaaacaaa aaaatttgca ggacagcttt ataactactt ggatacgaac tcaacagaga cctctatcgg tgagggtgtg tatgggtggg gtttatctct aggagcagat tcgaacactt atgaatatag aaatattttt gaggggcatg tgattgcaga cagctgggtc tctatcggta agggtgcata tgggtggggt ttatctctag gagcagattc gaacacttat gaatatggat ctgttgctat tggtcgaaat agcaatacac ctgttggtcg tggcgtagct ttaggtttta attctgtcaa taatcgttat gctgacaaga cttattataa ccctagaacg ggtgagattg atcaaagcag cgaatggacc ttggccagtg ccttagattc actgagtatt ggtaacgttg angcgggtac gacccgacag ttgacaggag tggcagcagg ctgggaagac accgatgcan tcaatgnanc acaactaaaa gcgttaanan agtgggcaga anaaagcttc tcnnggggga gantacctat gatgaanntt ctannntnnn tcnnnattgg ttcaaannnn tcgggannnn aannannncn ttnaaaannn cnatgggnna nnncnanntt

Expected Protein Sequence

MAHHHHHHTQ EVILDRKTKN DRKTKNDRKT ICILSLSVFD GNVVLAEDDD DYVCGLDQVS GFNVLDINPV EESLEKEGIS LTIKREDSAL KRLRALSGVF GVQSIRSGSH PYALGYDATI FDLGETKKFA GQLYNYLDTN STETSIGEGV YGWGLSLGAD SNTYEYRNIF EGHVIADSWV SIGKGAYGWG LSLGADSNTY EYGSVAIGRN SNTPVGRGVA LGFNSVNNRY ADKTYYNPRT GEIDQSSEWT LASALDSLSI GNVEAGTTRQ LTGVAAGWED TDAVNVAQLK ALREWAEESF SGGEITYDEG SKVIRIGSKR SGDKIDIKNS DGQDRVLAGV KDGEVSKGSV DAINGSQLWK TNQTVAGLNV TLNDLGKNLT NYLGGGADVV QNVAPTYTIQ GKQHRDVGSA FSGVDNILTD IYSKLGDLPG GGVKDQDALM WSDTENAFVA LHTKDGNKTN SKITYLLDGD ISKASTDAIN GSQLYMMSNK LAAYFGGGAG YENGQWSDPI FTLHQFNPDG NFVEKNYKTV ADAFGGVDTV IKDIYSKLGD LPGGGVKDQD ALMWSETENA FVALHGLEGK KTNSKLKFLL DGAIEQGSSE AITGNQLYMM SNKLAAYFGG GARYENGQWL DPTFMLHQLN PDGSVVEKSY KTVSDAFGGV DSSLSNLNDR LKAVEQGASP VPPSSDSDVL HWNEGKGGYD ASHKGQPGKI TEVAEGNIAQ GSKDAVNGGQ LWETNERLSG VEQKVDAIEQ GIGQVVDGAV SYDRDEHGNK TNKISLQGGN AGEPVVIDNV ADGKIEKGSK EAVNGGQLHD YTEQQMKVVL DDAKKYTDER IKNITVDALD DAVSQAKDYT DMK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacccagg aagttatttt ggatcgcaag actaagaatg atcgtaagac taagaatgat cgtaagacta tatgtattct ttctctaagt gtttttgatg gaaatgttgt tttagcagag gatgatgatg attatgtttg tggtttagat caggtttctg ggtttaatgt gttagatatc aacccagttg aggagagttt agagaaagag ggtatttcac ttacgataaa gagagaggat agcgctttga agaggctaag agctctctct ggagtttttg gtgttcagtc tattaggagc ggttcccatc cctatgcgct aggttatgat gcaacaatct ttgatctagg tgaaacaaaa aaatttgcag gacagcttta taactacttg gatacgaact caacagagac ctctatcggt gagggtgtgt atgggtgggg tttatctcta ggagcagatt cgaacactta tgaatataga aatatttttg aggggcatgt gattgcagac agctgggtct ctatcggtaa gggtgcatat gggtggggtt tatctctagg agcagattcg aacacttatg aatatggatc tgttgctatt ggtcgaaata gcaatacacc tgttggtcgt ggcgtagctt taggttttaa ttctgtcaat aatcgttatg ctgacaagac ttattataac cctagaacgg gtgagattga tcaaagcagc gaatggacct tggccagtgc cttagattca ctgagtattg gtaacgttga ggcgggtacg acccgacagt tgacaggagt ggcagcaggc tgggaagaca ccgatgcagt caatgtagca caactaaaag cgttaagaga gtgggcagaa gaaagcttct caggggggga gattacctat gatgaaggtt ctaaggttat tcgcattggt tcaaagcgct cgggagataa aatagacatt aaaaatagcg atgggcaaga tcgagttttg gctggagtga aggacgggga ggtttctaag gggtcagttg acgcaataaa tggcagtcaa ctttggaaaa ccaatcaaac agttgcgggt ctaaatgtaa ctttaaatga tctaggtaag aatctcacaa actatttagg tggtggtgca gatgtggttc agaatgttgc accaacttac actattcagg gcaaacagca tcgtgatgtt ggttctgctt ttagtggtgt tgataacata cttacagata tttatagtaa acttggggat ttgcctggtg gaggcgttaa ggatcaagat gccttaatgt ggagtgatac tgagaatgcc tttgttgcac ttcacacaaa agacggaaac aaaacgaaca gtaagattac ctatcttttg gatggtgaca ttagtaaagc ttcaacagat gccataaatg gttctcagct ttatatgatg agcaataagc ttgctgctta ttttggtggc ggtgctggat atgagaatgg gcagtggtcg gaccctatat ttacgcttca ccaattcaat cctgatggca attttgttga gaagaactat aagactgtag cggatgcttt tggtggtgtt gatacggtta tcaaggatat ttatagtaaa cttggggatt tgcctggtgg aggcgttaag gatcaagatg ccttaatgtg gagcgagact gagaatgctt ttgttgcgct tcatggattg gaaggaaaga agacgaatag caaactgaag tttctcttag atggtgccat tgagcaaggg tcgagtgaag cgattacggg taatcagctt tatatgatga gcaataagct tgctgcttat tttggtggcg gtgctagata tgagaatggg cagtggttgg accctacatt tatgcttcac caattgaatc ctgatggtag tgttgttgaa aagagctata agactgtatc ggatgctttt ggtggtgttg atagcagttt gtctaatctc aatgatcgtt tgaaggctgt tgaacaaggg gcatctccgg tacctccgtc ttctgattct gatgtgttac attggaatga agggaagggg gggtatgatg cgagccataa gggtcaacct ggtaagatta cggaagtagc tgagggtaat attgcgcaag gttcgaaaga tgcagtcaat ggtggacagc tttgggaaac caatgagcgt ctctctggtg ttgaacaaaa ggttgatgct attgagcaag ggattggtca ggttgttgat ggtgctgtta gctatgaccg agatgagcat gggaataaaa cgaataaaat cagtttacaa ggtggtaatg ctggtgaacc tgtggtgata gataatgttg cggatgggaa gatagagaag ggttcgaaag aagcggtgaa tggtggtcag ttgcatgact atacggaaca acagatgaag gttgttcttg atgatgcgaa aaaatatacg gatgaacgta tcaagaatat cactgttgat gcccttgatg atgctgtttc tcaagctaaa gactatacag atatgaagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for BaquA.17833.c.B4.GE36662

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	9kDa
OBSERVED MW:	9kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHMK ALREWAEESF SGGEITYDEG SKVIRIGSKR SGDKIDIKNS DGQDRVLAGV KDGEVSKGSV DAINGSQL

Validated NT Sequence

atcttactca cttaaagttg actgccattt attgcgtcaa ctgacccctt agaaacctcc ccgtccttca ctccagccaa aactcgatct tgcccatcgc tatttttaat gtctatttta tctcccgagc gctttgaacc aatgcgaata accttagaac cttcatcata ggtaatctcc ccccctgaga agctttcttc tgcccactct cttaacgctt tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHKA LREWAEESFS GGEITYDEGS KVIRIGSKRS GDKIDIKNSD GQDRVLAGVK DGEVSKGSVD AINGSQL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaagcgt taagagagtg ggcagaagaa agcttctcag ggggggagat tacctatgat gaaggttcta aggttattcg cattggttca aagcgctcgg gagataaaat agacattaaa aatagcgatg ggcaagatcg agttttggct ggagtgaagg acggggaggt ttctaagggg tcagttgacg caataaatgg cagtcaactt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for BaquA.17833.c.B5.GE36921

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	13kDa
OBSERVED MW:	13kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN DLGKNLTNYL GGGADVVQNV APTYTIQGKQ HRDVGSAFSG VDNILTDIYS KLGDLPGGGV KDQDALMWSD TENAFVALHT KDGNKTNSKI TYLLDGDISK ASTDAINGSQ L

Validated NT Sequence

atcttactca cttaaagctg agaaccattt atggcatctg ttgaagcttt actaatgtca ccatccaaaa gataggtaat cttactgttc gttttgtttc cgtcttttgt gtgaagtgca acaaaggcat tctcagtatc actccacatt aaggcatctt gatccttaac gcctccacca ggcaaatccc caagtttact ataaatatct gtaagtatgt tatcaacacc actaaaagca gaaccaacat cacgatgctg tttgccctga atagtgtaag ttggtgcaac attctgaacc acatctgcac caccacctaa atagtttgtg agattcttac ctagatcatt catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHND LGKNLTNYLG GGADVVQNVA PTYTIQGKQH RDVGSAFSGV DNILTDIYSK LGDLPGGGVK DQDALMWSDT ENAFVALHTK DGNKTNSKIT YLLDGDISKA STDAINGSQL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatgatc taggtaagaa tctcacaaac tatttaggtg gtggtgcaga tgtggttcag aatgttgcac caacttacac tattcagggc aaacagcatc gtgatgttgg ttctgctttt agtggtgttg ataacatact tacagatatt tatagtaaac ttggggattt gcctggtgga ggcgttaagg atcaagatgc cttaatgtgg agtgatactg agaatgcctt tgttgcactt cacacaaaag acggaaacaa aacgaacagt aagattacct atcttttgga tggtgacatt agtaaagctt caacagatgc cataaatggt tctcagcttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BaquA.17833.c.B7.GE37092

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	14kDa
OBSERVED MW:	15kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMY MMSNKLAAYF GGGARYENGQ WLDPTFMLHQ LNPDGSVVEK SYKTVSDAFG GVDSSLSNLN DRLKAVEQGA SPVPPSSDSD VLHWNEGKGG YDASHKGQPG KITEVAEGNI AQGSKDAVNG GQL

Validated NT Sequence

atcttactca cttaaagctg tccaccattg actgcatctt tcgaaccttg cgcaatatta ccctcagcta cttccgtaat cttaccaggt tgacccttat ggctcgcatc ataccccccc ttcccttcat tccaatgtaa cacatcagaa tcagaagacg gaggtaccgg agatgcccct tgttcaacag ccttcaaacg atcattgaga ttagacaaac tgctatcaac accaccaaaa gcatccgata cagtcttata gctcttttca acaacactac catcaggatt caattggtga agcataaatg tagggtccaa ccactgccca ttctcatatc tagcaccgcc accaaaataa gcagcaagct tattgctcat catatacata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHYM MSNKLAAYFG GGARYENGQW LDPTFMLHQL NPDGSVVEKS YKTVSDAFGG VDSSLSNLND RLKAVEQGAS PVPPSSDSDV LHWNEGKGGY DASHKGQPGK ITEVAEGNIA QGSKDAVNGG QL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattatatga tgagcaataa gcttgctgct tattttggtg gcggtgctag atatgagaat gggcagtggt tggaccctac atttatgctt caccaattga atcctgatgg tagtgttgtt gaaaagagct ataagactgt atcggatgct tttggtggtg ttgatagcag tttgtctaat ctcaatgatc gtttgaaggc tgttgaacaa ggggcatctc cggtacctcc gtcttctgat tctgatgtgt tacattggaa tgaagggaag ggggggtatg atgcgagcca taagggtcaa cctggtaaga ttacggaagt agctgagggt aatattgcgc aaggttcgaa agatgcagtc aatggtggac agctttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BaquA.17833.c.B8.GE36838

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	9kDa
OBSERVED MW:	9kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	102

Validated AA Sequence

MAHHHHHHHM ETNERLSGVE QKVDAIEQGI GQVVDGAVSY DRDEHGNKTN KISLQGGNAG EPVVIDNVAD GKIEKGSKEA VNGGQL

Validated NT Sequence

tttntnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc accatatgga aaccaatgag cgtctctctg gtgttgaaca aaaggttgat gctattgagc aagggattgg tcaggttgtt gatggtgctg ttagctatga ccgagatgag catgggaata aaacgaataa aatcagttta caaggtggta atgctggtga acctgtggtg atagataatg ttgcggatgg gaagatagag aagggttcga aagaagcggt gaatggtggt cagttgtaag tgagtaagat aggatccggc tgctaacnnn cccgaaagan nnnnnnnnna annnnannnn nnnnnnnncn cnnnnnnnnn nnnnntgnnn ntnnnnnnnn gnnnannnnn nnnnnncntg ntgnnntcnn nnngnnccnn nnnnnnnngn ncnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncnnngnnn nnnnnnngnn ncntgnnnnn nnnnnnnnnn nctnnnnnnn nnangcnnnn nnnnnnnnnn nnnnnnannn ccnnnggnnc nnnnncnnnn nnnnnnnnnn nnnnnnnnnn nnnnannnnn ntncnnnnnn cnnnnnnnnn nnnnnnnnan ntnntnanct nncctccnnn nntatggncn nnnnctnnnt tccnnntnnn nnnnnnnnac nnnntnnnnc gnantnnnan nnnnnnnntc ncnannt

Expected Protein Sequence

MAHHHHHHET NERLSGVEQK VDAIEQGIGQ VVDGAVSYDR DEHGNKTNKI SLQGGNAGEP VVIDNVADGK IEKGSKEAVN GGQL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaacca atgagcgtct ctctggtgtt gaacaaaagg ttgatgctat tgagcaaggg attggtcagg ttgttgatgg tgctgttagc tatgaccgag atgagcatgg gaataaaacg aataaaatca gtttacaagg tggtaatgct ggtgaacctg tggtgataga taatgttgcg gatgggaaga tagagaaggg ttcgaaagaa gcggtgaatg gtggtcagtt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BaquA.17833.c.B9.GE36839

HARVESTED ON:	5/1/2013
SEQUENCED ON:	5/8/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME DSALKRLRAL SGVFGVQSIR SGSHPYALGY DATIFDLGET KKFAGQLYNY LDTNSTETSI GEGVYGWGLS LGADSNTYEY RNIFEGHVIA DSWVSIGKGA YGWGLSLGAD SNTYEYGSVA IGRNSNTPVG RGVALGFNSV NNRYADKTYY NPRTGEIDQS SEWTLASALD SLSIGNVEAG TTRQLTGVAA GWEDTDAVNV AQLKALREWA EESFSGGEIT YDEGSKVIRI GSKRSGDKID IKNSDGQDRV LAGVKDGEVS KGSVDAINGS QLWKTNQTVA GLNVTLNDLG KNLTN

Validated NT Sequence

atcttactca cttagtttgt gagattctta cctagatcat ttaaagttac atttagaccc gcaactgttt gattggtttt ccaaagttga ctgccattta ttgcgtcaac tgacccctta gaaacctccc cgtccttcac tccagccaaa actcgatctt gcccatcgct atttttaatg tctattttat ctcccgagcg ctttgaacca atgcgaataa ccttagaacc ttcatcatag gtaatctccc cccctgagaa gctttcttct gcccactctc ttaacgcttt tagttgtgct acattgactg catcggtgtc ttcccagcct gctgccactc ctgtcaactg tcgggtcgta cccgcctcaa cgttaccaat actcagtgaa tctaaggcac tggccaaggt ccattcgctg ctttgatcaa tctcacccgt tctagggtta taataagtct tgtcagcata acgattattg acagaattaa aacctaaagc tacgccacga ccaacaggtg tattgctatt tcgaccaata gcaacagatc catattcata agtgttcgaa tctgctccta gagataaacc ccacccatat gcacccttac cgatagagac ccagctgtct gcaatcacat gcccctcaaa aatatttcta tattcataag tgttcgaatc tgctcctaga gataaacccc acccatacac accctcaccg atagaggtct ctgttgagtt cgtatccaag tagttataaa gctgtcctgc aaattttttt gtttcaccta gatcaaagat tgttgcatca taacctagcg catagggatg ggaaccgctc ctaatagact gaacaccaaa aactccagag agagctctta gcctcttcaa agcgctatcc tccatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHED SALKRLRALS GVFGVQSIRS GSHPYALGYD ATIFDLGETK KFAGQLYNYL DTNSTETSIG EGVYGWGLSL GADSNTYEYR NIFEGHVIAD SWVSIGKGAY GWGLSLGADS NTYEYGSVAI GRNSNTPVGR GVALGFNSVN NRYADKTYYN PRTGEIDQSS EWTLASALDS LSIGNVEAGT TRQLTGVAAG WEDTDAVNVA QLKALREWAE ESFSGGEITY DEGSKVIRIG SKRSGDKIDI KNSDGQDRVL AGVKDGEVSK GSVDAINGSQ LWKTNQTVAG LNVTLNDLGK NLTN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaggata gcgctttgaa gaggctaaga gctctctctg gagtttttgg tgttcagtct attaggagcg gttcccatcc ctatgcgcta ggttatgatg caacaatctt tgatctaggt gaaacaaaaa aatttgcagg acagctttat aactacttgg atacgaactc aacagagacc tctatcggtg agggtgtgta tgggtggggt ttatctctag gagcagattc gaacacttat gaatatagaa atatttttga ggggcatgtg attgcagaca gctgggtctc tatcggtaag ggtgcatatg ggtggggttt atctctagga gcagattcga acacttatga atatggatct gttgctattg gtcgaaatag caatacacct gttggtcgtg gcgtagcttt aggttttaat tctgtcaata atcgttatgc tgacaagact tattataacc ctagaacggg tgagattgat caaagcagcg aatggacctt ggccagtgcc ttagattcac tgagtattgg taacgttgag gcgggtacga cccgacagtt gacaggagtg gcagcaggct gggaagacac cgatgcagtc aatgtagcac aactaaaagc gttaagagag tgggcagaag aaagcttctc agggggggag attacctatg atgaaggttc taaggttatt cgcattggtt caaagcgctc gggagataaa atagacatta aaaatagcga tgggcaagat cgagttttgg ctggagtgaa ggacggggag gtttctaagg ggtcagttga cgcaataaat ggcagtcaac tttggaaaac caatcaaaca gttgcgggtc taaatgtaac tttaaatgat ctaggtaaga atctcacaaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BaquA.17833.c.MB3.GE38119

HARVESTED ON:	6/6/2014
SEQUENCED ON:	6/19/2014
EXPECTED MW:	55kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	57

Validated AA Sequence

MNADTDYSIA EAAFXKGETA MTINGPWAWS NIDTSKVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDEALKD AQTNSSSNNN NNNNNNNLGI EGRISEGTLE AQTQGPGSMS NTYEYGSVAI GRNSNTPVGR GVALGFNSVN NRYADKTYYN PRTGEIDQSS EWTLASALDS LSIGNVEAGT TRQLTGVAAG WEDTDAVNV

Validated NT Sequence

tgctacattg actgcatcgg tgtcttccca gcctgctgcc actcctgtca actgtcgggt cgtacccgcc tcaacgttac caatactcag tgaatctaag gcactggcca aggtccattc gctgctttga tcaatctcac ccgttctagg gttataataa gtcttgtcag cataacgatt attgacagaa ttaaaaccta aagctacgcc acgaccaaca ggtgtattgc tatttcgacc aatagcaaca gatccatatt cataagtgtt cgacatcgaa ccaggaccct gggtctgagc ttccagggta ccttctgaaa tccttccctc gatcccgagg ttgttgttat tgttgttgtt gttgttgttc gagctcgaat tagtctgcgc gtctttcagg gcttcatcga cagtctgacg accgctggcg gcgttgatca ccgcagtacg cacggcatac cagaaagcgg acatctgcgg gatgttcggc atgatttcac ctttctgggc gttttccata gtggcggcaa tacgtggatc tttcgccaac tcttcctcgt aagacttcag cgctacggca cccagcggtt tgtctttatt aaccgcttcc agaccttcat cagtcagcag atagttttcg aggaactctt ttgccagctc tttgttcgga ctggcggcgt taatacctgc gctcagcacg ccaacgaacg gtttggatgg ttgacccttg aaggtcggca gtaccgttac accataattc actttgctgg tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat cgctgtttcg cctttatnaa angcagcttc tgcgatggag taatcggtgt ctgcattcat gtgtttgttt ttaatcnntc aaccnnnnnn gtcanannnn ctttcgcgnn nncgnnntcc acnnc

Expected Protein Sequence

MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS SNTYEYGSVA IGRNSNTPVG RGVALGFNSV NNRYADKTYY NPRTGEIDQS SEWTLASALD SLSIGNVEAG TTRQLTGVAA GWEDTDAVNV AQL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgtcgaa cacttatgaa tatggatctg ttgctattgg tcgaaatagc aatacacctg ttggtcgtgg cgtagcttta ggttttaatt ctgtcaataa tcgttatgct gacaagactt attataaccc tagaacgggt gagattgatc aaagcagcga atggaccttg gccagtgcct tagattcact gagtattggt aacgttgagg cgggtacgac ccgacagttg acaggagtgg cagcaggctg ggaagacacc gatgcagtca atgtagcaca actataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BaquA.17833.c.MB9.GE38120

HARVESTED ON:	6/6/2014
SEQUENCED ON:	6/19/2014
EXPECTED MW:	77kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	42

Validated AA Sequence

MEDSALKRLR ALSGVFGVQS IRSGSHPYAL GYDATIFDLG ETKKFAGQLY NYLDTNSTET SIGEGVYGWG LSLGADSNTY EYRNIFEGHV IADSWVSIGK GAYGWGLSLG ADSNTYEYGS VAIGRNSNTP VGRGVALGFN SVNNRYADKT YYNPRTGEID QSSEWTLASA LDSLSIGNVE AGTTRQLTGV AAGWEDTDAV NVAQLKALRE WAEESFSGGE ITYDEGSKVI RIGSKRSGDK IDIKNSDGQD RVLAGVKDGE VSKGSVDAIN GSQLWKTNQT VAGLNVTLND LGKNLTN

Validated NT Sequence

gantngagna ccntggagct cagacccagg gtcctggttc gatggaggat agcgctttga agaggctaag agctctctct ggagtttttg gtgttcagtc tattaggagc ggttcccatc cctatgcgct aggttatgat gcaacaatct ttgatctagg tgaaacaaaa aaatttgcag gacagcttta taactacttg gatacgaact caacagagac ctctatcggt gagggtgtgt atgggtgggg tttatctcta ggagcagatt cgaacactta tgaatataga aatatttttg aggggcatgt gattgcagac agctgggtct ctatcggtaa gggtgcatat gggtggggtt tatctctagg agcagattcg aacacttatg aatatggatc tgttgctatt ggtcgaaata gcaatacacc tgttggtcgt ggcgtagctt taggttttaa ttctgtcaat aatcgttatg ctgacaagac ttattataac cctagaacgg gtgagattga tcaaagcagc gaatggacct tggccagtgc cttagattca ctgagtattg gtaacgttga ggcgggtacg acccgacagt tgacaggagt ggcagcaggc tgggaagaca ccgatgcagt caatgtagca caactaaaag cgttaagaga gtgggcagaa gaaagcttct caggggggga gattacctat gatgaaggtt ctaaggttat tcgcattggt tcaaagcgct cgggagataa aatagacatt aaaaatagcg atgggcaaga tcgagttttg gctggagtga aggacgggga ggtttctaag gggtcagttg acgcaataaa tggcagtcaa ctttggaaaa ccaatcaaac agttgcgggt ctaaatgtaa ctttaaatga tctaggtaag aatctcacaa actaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccncgctgag catactagca taccctgggg cctcnacnnn nnnnnagggg nnnnnnnnnt tnnnntnnng gnnnnnnnac nnnnnccnnn nnntcncccn angtcatnan tcntnngana annnnnanna antnnnnnnn nannnnnann nnnannnnnn nnnanntnnt ntgnnnngan nnnnagnnnt nnngtnnnnn nannganncg aggnntg

Expected Protein Sequence

MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS EDSALKRLRA LSGVFGVQSI RSGSHPYALG YDATIFDLGE TKKFAGQLYN YLDTNSTETS IGEGVYGWGL SLGADSNTYE YRNIFEGHVI ADSWVSIGKG AYGWGLSLGA DSNTYEYGSV AIGRNSNTPV GRGVALGFNS VNNRYADKTY YNPRTGEIDQ SSEWTLASAL DSLSIGNVEA GTTRQLTGVA AGWEDTDAVN VAQLKALREW AEESFSGGEI TYDEGSKVIR IGSKRSGDKI DIKNSDGQDR VLAGVKDGEV SKGSVDAING SQLWKTNQTV AGLNVTLNDL GKNLTN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcggagga tagcgctttg aagaggctaa gagctctctc tggagttttt ggtgttcagt ctattaggag cggttcccat ccctatgcgc taggttatga tgcaacaatc tttgatctag gtgaaacaaa aaaatttgca ggacagcttt ataactactt ggatacgaac tcaacagaga cctctatcgg tgagggtgtg tatgggtggg gtttatctct aggagcagat tcgaacactt atgaatatag aaatattttt gaggggcatg tgattgcaga cagctgggtc tctatcggta agggtgcata tgggtggggt ttatctctag gagcagattc gaacacttat gaatatggat ctgttgctat tggtcgaaat agcaatacac ctgttggtcg tggcgtagct ttaggtttta attctgtcaa taatcgttat gctgacaaga cttattataa ccctagaacg ggtgagattg atcaaagcag cgaatggacc ttggccagtg ccttagattc actgagtatt ggtaacgttg aggcgggtac gacccgacag ttgacaggag tggcagcagg ctgggaagac accgatgcag tcaatgtagc acaactaaaa gcgttaagag agtgggcaga agaaagcttc tcaggggggg agattaccta tgatgaaggt tctaaggtta ttcgcattgg ttcaaagcgc tcgggagata aaatagacat taaaaatagc gatgggcaag atcgagtttt ggctggagtg aaggacgggg aggtttctaa ggggtcagtt gacgcaataa atggcagtca actttggaaa accaatcaaa cagttgcggg tctaaatgta actttaaatg atctaggtaa gaatctcaca aactaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

CENTER ID:	BaquA.17833.c
ORGANISM:	Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

PURIFICATION DATe:	7/15/2013
CONCENTRATION:	19.2mg/ml
OBSERVED MW:	11kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

BaquA.17833.c

VompB

Ordering Clones & Proteins

Clones*

Proteins

External Resources

Sequences

AA Sequence

NT Sequence

PURIFICATION DATe:	4/8/2013
CONCENTRATION:	13.8mg/ml
OBSERVED MW:	9kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	data unavailable
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	101