BaquA.17833.b
VompD
| CENTER ID: | BaquA.17833.b |
| ORGANISM: | Bartonella quintana Toulouse |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaquA.17833.b.B10.GE37342 | VompD:aa52-375 (MV:239-333) | 52 | 375 | ||
| BaquA.17833.b.B11.GE37343 | VompD:aa144-332 (MV:239-333) | 144 | 332 | ||
| BaquA.17833.b.B2.GE36657 | mature protein, soluble domain | 33 | 853 | ||
| BaquA.17833.b.B2.GE37344 | mature protein, soluble domain | 33 | 853 | ||
| BaquA.17833.b.B4.GE36659 | VompD:aa214-340 (MV:239-333) | 214 | 340 | ||
| BaquA.17833.b.B9.GE36836 | VompD:aa715-808 | 715 | 808 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaquA.17833.b.B4.PW37282 | VompD:aa214-340 (MV:239-333) | 214 | 340 | ||
| BaquA.17833.b.B9.PW37313 | VompD:aa715-808 | 715 | 808 |
Structures
4LGO
DEPOSITED: 6/28/2013
DETERMINATION: XRay
CLONE: BaquA.17833.b.B4.GE36659
PROTEIN: BaquA.17833.b.B4.PW37282
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| UniProt: | Q64HT0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKLYSTQNW AKVASLGTAM AALLSGVSPV FAAGVSITEG FVRNSSGTDG PYPKRSYASI VFAGDDNYCG VDNVIGRGGS TQGSNNQITA EEQYNRFIED RTFNGRNPYS TTTEKVTWVK DGVLSNNSGS YMGTATGGLQ SALPEAYGVY SFATGCGFSA TGNYSTAFGA EATGKAGGAQ AFSVGDTEND ITRQITGVAA GTSGTDAMNV APLKAMKDLV VGAWKLVVND ENPIDVNAGS TVKFVGVKAE EGNEDSKNIK ITTGNNNEVK FDLNDIIRVK RVIAGKANVS EVGFVITGGP NMTVGGINAG NKKITGVANG IRENDAVNVS QLNELKNQIA GGSLVNWLEG QKLITVGAEK GGTEINIAGS EGNRTIAGVK AAEKDNEAVN KKQLDDHIAD ITDIINNIKT ESAFAVLYDK NGDNTVNYNS VTLGGGKSDG PVALLNVKDG KITKESHDAI NGSQINKISG DIAKFFGGDA AFEGGVFAGP RYNLSEVFAD GNVKQKVFED VGSALTGLDM NVRSVNNRLT YVANSVDQQI ENFSKNALIW SDTENAFVAL HTNDGNKTES KITYLLDGDI SKASTDAING SQLYMMSNKL AAYFGGGAGY ENGQWSDPTF MLQQLNPDGS VVEKNYKTVA DAFGSVDSSL SNLNNRLKAV EQGASPVPPS SDSDVLHWNE EKGGYDASHK GQPGKITEVA EGNIAQGSKD AVNGGQLWET NERLSGVEKD VQHISDRVDN ISNTIADIGD IVNNMADGAV SYDRDEHGNK TNKISLQGGN AGEPVVIDNV ADGKIEKGSK EAVNGGQLHD YTEQQMKVVL DDAKKYTDER IKNITVDAID DAVSQAKDYT DMRFEALNYG IEGARKEARQ AAAIGLAVSN LRYNDTPGKL SIAFGSGLWR SQGAFAFGAG YTSESGAIRS NLSVTSSGGH WGIGAGLGLT LN
NT Sequence
atgaaaaaat tatattcaac acaaaattgg gctaaagttg cctcattggg aacggcaatg gcagcgctct tatcgggtgt ctctcctgtt ttcgctgcgg gtgtttccat aacagaagga tttgttcgaa attcgagtgg cactgatggt ccttatccaa aaaggagtta tgccagtatt gtttttgctg gtgatgataa ttactgtggt gtagataatg ttattggtcg tggtggcagt actcagggct ctaataatca aataacggca gaggagcaat ataacagatt tatcgaagat agaacattta atggccgcaa cccttatagc acaaccactg aaaaagttac ttgggttaaa gatggagtct tgagtaacaa cagtggtagt tatatgggaa cagccactgg tggtcttcag agtgctctac ctgaggctta tggtgtttat tcttttgcta ccggttgtgg attttctgcg acgggaaact attcaacagc gtttggtgca gaagcgacgg gaaaggctgg tggcgcgcaa gcttttagtg ttggcgatac tgagaatgat ataacacgac aaattacagg agtggcagca gggacctctg gtaccgatgc aatgaatgtg gcaccgctaa aagctatgaa agatttggta gtaggagctt ggaaacttgt tgttaacgat gaaaatccta tagatgttaa tgcaggcagt acagtaaaat ttgtaggtgt taaggccgaa gagggtaatg aagatagtaa aaatatcaaa attaccacag gaaataataa tgaggtaaaa tttgatctaa atgatattat cagagttaaa cgtgtaatag caggcaaggc gaacgtaagt gaagtgggtt ttgtgattac tggtggccca aacatgactg ttggtggtat taatgccgga aataaaaaaa ttacaggagt agcaaatggt attcgagaga atgatgcagt aaatgtttca cagttgaacg aactaaaaaa tcaaatagca ggaggtagtc ttgttaattg gttggaaggg cagaagctta ttactgttgg tgctgaaaaa ggtggcacgg aaattaatat tgcaggcagt gaaggaaatc ggactatcgc cggtgtgaaa gcagcagaaa aagataatga agctgttaat aaaaaacagc ttgatgacca tattgcagat attactgata ttattaacaa tattaagaca gaaagtgctt ttgcggttct ctatgataaa aatggtgata acaccgttaa ttataatagt gtgactttgg gcgggggtaa gagcgatgga cccgttgctc tccttaatgt caaagatggc aaaattacca aagaatcgca tgatgcaatc aatggtagcc aaattaataa gatatctgga gatattgcaa aattttttgg cggtgatgcg gcatttgaag gtggtgtctt tgcgggccct agatataatt tatctgaagt ttttgcagat ggtaatgtaa aacaaaaggt ctttgaagat gttggctcag cgttgacagg gcttgatatg aatgtcagaa gtgtgaataa ccgtctaaca tatgtagcca atagcgttga tcagcaaatt gaaaattttt ctaaaaatgc tttaatatgg agcgatactg agaatgcctt tgttgcgctt catacaaacg acggaaacaa aacggagagt aagattacct atcttttgga tggtgacatt agtaaagctt caacagatgc cataaatggt tctcagcttt atatgatgag caataagctt gctgcttatt ttggtggcgg tgctggatat gagaatgggc agtggtcgga tccaacattt atgcttcagc aattgaatcc tgatggtagt gttgttgaga agaactataa gactgtagcg gatgcttttg gtagtgttga tagcagtttg tctaatctca ataatcgttt gaaggctgtt gaacaagggg catctccggt acctccgtct tctgattctg atgtgttaca ttggaatgaa gagaaagggg ggtatgatgc gagccataag ggtcaacctg gtaagattac ggaagtagct gagggtaata ttgcgcaagg ttcgaaagat gcagtcaatg gtggacagct ttgggaaacc aatgagcgtc tctctggtgt tgaaaaagat gttcagcata tttcagacag agtggacaat atttccaata cgattgctga tattggtgat atagttaaca acatggctga tggtgctgtt agctatgacc gagatgagca tgggaataaa acgaataaaa tcagtttaca aggtggtaat gctggtgaac ctgtggtgat agataatgtt gcggatggta agatagagaa gggttcgaaa gaagcggtga atggtggtca gttgcatgac tatacggaac aacagatgaa ggttgttctt gatgatgcga aaaaatatac ggatgaacgt atcaagaata tcactgttga tgccattgat gatgctgttt ctcaagctaa agactataca gatatgaggt ttgaggcttt aaattatggc attgagggtg cccggaaaga agcgaggcaa gcagcggcta taggtttagc ggtatctaac ttacgttaca atgatacacc tggaaaatta agcattgcat ttgggagcgg cttatggcgt agtcaaggtg catttgcctt tggtgcgggg tatacatctg aaagtggggc tattcggtcc aatttatctg tcaccagttc tggtggtcat tggggaatag gtgctggatt aggtttaaca ctgaattga
Details for BaquA.17833.b.B10.GE37342
| HARVESTED ON: | 9/27/2013 |
| SEQUENCED ON: | 10/28/2013 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMY PKRSYASIVF AGDDNYCGVD NVIGRGGSTQ GSNNQITAEE QYNRFIEDRT FNGRNPYSTT TEKVTWVKDG VLSNNSGSYM GTATGGLQSA LPEAYGVYSF ATGCGFSATG NYSTAFGAEA TGKAGGAQAF SVGDTENDIT RQITGVAAGT SGTDAMNVAP LKAMKDLVVG AWKLVVNDEN PIDVNAGSTV KFVGVKAEEG NEDSKNIKIT TGNNNEVKFD LNDIIRVKRV IAGKANVSEV GFVITGGPNM TVGGINAGNK KITGVANGIR ENDAVNVSQL NELKNQIAGG SLVNWLEGQK LITVGAEKGG TXXNIAGSEG NRT
Validated NT Sequence
tactcactta agtccgattt ccttcactgc ctgcaatatt annnnnngtg ccaccttttt cagcaccaac agtaataagc ttctgccctt ccaaccaatt aacaagacta cctcctgcta tttgattttt tagttcgttc aactgtgaaa catttactgc atcattctct cgaataccat ttgctactcc tgtaattttt ttatttccgg cattaatacc accaacagtc atgtttgggc caccagtaat cacaaaaccc acttcactta cgttcgcctt gcctgctatt acacgtttaa ctctgataat atcatttaga tcaaatttta cctcattatt atttcctgtg gtaattttga tatttttact atcttcatta ccctcttcgg ccttaacacc tacaaatttt actgtactgc ctgcattaac atctatagga ttttcatcgt taacaacaag tttccaagct cctactacca aatctttcat agcttttagc ggtgccacat tcattgcatc ggtaccagag gtccctgctg ccactcctgt aatttgtcgt gttatatcat tctcagtatc gccaacacta aaagcttgcg cgccaccagc ctttcccgtc gcttctgcac caaacgctgt tgaatagttt cccgtcgcag aaaatccaca accggtagca aaagaataaa caccataagc ctcaggtaga gcactctgaa gaccaccagt ggctgttccc atataactac cactgttgtt actcaagact ccatctttaa cccaagtaac tttttcagtg gttgtgctat aagggttgcg gccattaaat gttctatctt cgataaatct gttatattgc tcctctgccg ttatttgatt attagagccc tgagtactgc caccacgacc aataacatta tctacaccac agtaattatc atcaccagca aaaacaatac tggcataact cctttttgga tacatatggt ggtggtggtg gtgagccatg gta
Expected Protein Sequence
MAHHHHHHYP KRSYASIVFA GDDNYCGVDN VIGRGGSTQG SNNQITAEEQ YNRFIEDRTF NGRNPYSTTT EKVTWVKDGV LSNNSGSYMG TATGGLQSAL PEAYGVYSFA TGCGFSATGN YSTAFGAEAT GKAGGAQAFS VGDTENDITR QITGVAAGTS GTDAMNVAPL KAMKDLVVGA WKLVVNDENP IDVNAGSTVK FVGVKAEEGN EDSKNIKITT GNNNEVKFDL NDIIRVKRVI AGKANVSEVG FVITGGPNMT VGGINAGNKK ITGVANGIRE NDAVNVSQLN ELKNQIAGGS LVNWLEGQKL ITVGAEKGGT EINIAGSEGN RT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattatccaa aaaggagtta tgccagtatt gtttttgctg gtgatgataa ttactgtggt gtagataatg ttattggtcg tggtggcagt actcagggct ctaataatca aataacggca gaggagcaat ataacagatt tatcgaagat agaacattta atggccgcaa cccttatagc acaaccactg aaaaagttac ttgggttaaa gatggagtct tgagtaacaa cagtggtagt tatatgggaa cagccactgg tggtcttcag agtgctctac ctgaggctta tggtgtttat tcttttgcta ccggttgtgg attttctgcg acgggaaact attcaacagc gtttggtgca gaagcgacgg gaaaggctgg tggcgcgcaa gcttttagtg ttggcgatac tgagaatgat ataacacgac aaattacagg agtggcagca gggacctctg gtaccgatgc aatgaatgtg gcaccgctaa aagctatgaa agatttggta gtaggagctt ggaaacttgt tgttaacgat gaaaatccta tagatgttaa tgcaggcagt acagtaaaat ttgtaggtgt taaggccgaa gagggtaatg aagatagtaa aaatatcaaa attaccacag gaaataataa tgaggtaaaa tttgatctaa atgatattat cagagttaaa cgtgtaatag caggcaaggc gaacgtaagt gaagtgggtt ttgtgattac tggtggccca aacatgactg ttggtggtat taatgccgga aataaaaaaa ttacaggagt agcaaatggt attcgagaga atgatgcagt aaatgtttca cagttgaacg aactaaaaaa tcaaatagca ggaggtagtc ttgttaattg gttggaaggg cagaagctta ttactgttgg tgctgaaaaa ggtggcacgg aaattaatat tgcaggcagt gaaggaaatc ggacttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BaquA.17833.b.B11.GE37343
| HARVESTED ON: | 9/27/2013 |
| SEQUENCED ON: | 10/3/2013 |
| EXPECTED MW: | 20kDa |
| OBSERVED MW: | 20kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMP ESYGVYSFAT GCGFSATGNY STAFGAEATG KAGGAQAFSV GDTENDITRQ ITGVAAGTSG TDAMNVAPLK AMKDLVVGAW KLVVNDENPI DVNAGSTVKF VGVKAEEGNE DSKNIKITTG NNNEVKFDLN DIIRVKRVIA GKANVSEVGF VITGGPNMTV GGINAGNKKI TGVANGIREN DAVNVSQL
Validated NT Sequence
cagccggatc ctatcttact cacttacaac tgtgaaacat ttactgcatc attctctcga ataccatttg ctactcctgt aattttttta tttccggcat taataccacc aacagtcatg tttgggccac cagtaatcac aaaacccact tcacttacgt tcgccttgcc tgctattaca cgtttaactc tgataatatc atttagatca aattttacct cattattatt tcctgtggta attttgatat ttttactatc ttcattaccc tcttcggcct taacacctac aaattttact gtactgcctg cattaacatc tataggattt tcatcgttaa caacaagttt ccaagctcct actaccaaat ctttcatagc ttttagcggt gccacattca ttgcatcggt accagaggtc cctgctgcca ctcctgtaat ttgtcgtgtt atatcattct cagtatcgcc aacactaaaa gcttgcgcgc caccagcctt tcccgtcgct tctgcaccaa acgctgttga atagtttccc gtcgcagaaa atccacaacc ggtagcaaaa gaataaacac cataagactc aggcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHPE AYGVYSFATG CGFSATGNYS TAFGAEATGK AGGAQAFSVG DTENDITRQI TGVAAGTSGT DAMNVAPLKA MKDLVVGAWK LVVNDENPID VNAGSTVKFV GVKAEEGNED SKNIKITTGN NNEVKFDLND IIRVKRVIAG KANVSEVGFV ITGGPNMTVG GINAGNKKIT GVANGIREND AVNVSQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctgagg cttatggtgt ttattctttt gctaccggtt gtggattttc tgcgacggga aactattcaa cagcgtttgg tgcagaagcg acgggaaagg ctggtggcgc gcaagctttt agtgttggcg atactgagaa tgatataaca cgacaaatta caggagtggc agcagggacc tctggtaccg atgcaatgaa tgtggcaccg ctaaaagcta tgaaagattt ggtagtagga gcttggaaac ttgttgttaa cgatgaaaat cctatagatg ttaatgcagg cagtacagta aaatttgtag gtgttaaggc cgaagagggt aatgaagata gtaaaaatat caaaattacc acaggaaata ataatgaggt aaaatttgat ctaaatgata ttatcagagt taaacgtgta atagcaggca aggcgaacgt aagtgaagtg ggttttgtga ttactggtgg cccaaacatg actgttggtg gtattaatgc cggaaataaa aaaattacag gagtagcaaa tggtattcga gagaatgatg cagtaaatgt ttcacagttg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BaquA.17833.b.B2.GE36657
| HARVESTED ON: | 2/27/2013 |
| SEQUENCED ON: | 2/2/2013 |
| EXPECTED MW: | 89kDa |
| OBSERVED MW: | 89kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 36 |
Validated AA Sequence
MAHHHHHHMA GVSITEGFVR NSSGTDGPYP KRSYASIVFA GDDNYCGVDN VIGRGGSTQG SNNQITAEEQ YNRFIEDRTF NGRNPYSTTT EKVTWVKDGV LSNNSGSYMG TATGGLQSAL PEAYGVYSFA TGCGFSATGN YSTAFGAEAT GKAGGAQAFS VGDTENDITR QITGVAAGTS GTDAMNVAPL KAMKDLVVGA WKLVVNDENP IDVNAGSTVK FVGVKAEEGN EDSKNIKITT GNNNEVKFDL NDIIRVKRVI AGKANVSEVG FVITGGPNMT VGGINAGNKK ITGVANGIRE ND
Validated NT Sequence
atggctcacc accaccacca ccatatggcg ggtgtttcca taacagaagg atttgttcga aattcgagtg gcactgatgg tccttatcca aaaaggagtt atgccagtat tgtttttgct ggtgatgata attactgtgg tgtagataat gttattggtc gtggtggcag tactcagggc tctaataatc aaataacggc agaggagcaa tataacagat ttatcgaaga tagaacattt aatggccgca acccttatag cacaaccact gaaaaagtta cttgggttaa agatggagtc ttgagtaaca acagtggtag ttatatggga acagccactg gtggtcttca gagtgctcta cctgaggctt atggtgttta ttcttttgct accggttgtg gattttctgc gacgggaaac tattcaacag cgtttggtgc agaagcgacg ggaaaggctg gtggcgcgca agcttttagt gttggcgata ctgagaatga tataacacga caaattacag gagtggcagc agggacctct ggtaccgatg caatgaatgt ggcaccgcta aaagctatga aagatttggt agtaggagct tggaaacttg ttgttaacga tgaaaatcct atagatgtta atgcaggcag tacagtaaaa tttgtaggtg ttaaggccga agagggtaat gaagatagta aaaatatcaa aattaccaca ggaaataata atgaggtaaa atttgatcta aatgatatta tcagagttaa acgtgtaata gcaggcaagg cgaacgtaag tgaagtgggt tttgtgatta ctggtggccc aaacatgact gttggtggta ttaatgccgg aaataaaaaa attacaggag tagcaaatgg tattcgagag aatgatgc
Expected Protein Sequence
MAHHHHHHAG VSITEGFVRN SSGTDGPYPK RSYASIVFAG DDNYCGVDNV IGRGGSTQGS NNQITAEEQY NRFIEDRTFN GRNPYSTTTE KVTWVKDGVL SNNSGSYMGT ATGGLQSALP EAYGVYSFAT GCGFSATGNY STAFGAEATG KAGGAQAFSV GDTENDITRQ ITGVAAGTSG TDAMNVAPLK AMKDLVVGAW KLVVNDENPI DVNAGSTVKF VGVKAEEGNE DSKNIKITTG NNNEVKFDLN DIIRVKRVIA GKANVSEVGF VITGGPNMTV GGINAGNKKI TGVANGIREN DAVNVSQLNE LKNQIAGGSL VNWLEGQKLI TVGAEKGGTE INIAGSEGNR TIAGVKAAEK DNEAVNKKQL DDHIADITDI INNIKTESAF AVLYDKNGDN TVNYNSVTLG GGKSDGPVAL LNVKDGKITK ESHDAINGSQ INKISGDIAK FFGGDAAFEG GVFAGPRYNL SEVFADGNVK QKVFEDVGSA LTGLDMNVRS VNNRLTYVAN SVDQQIENFS KNALIWSDTE NAFVALHTND GNKTESKITY LLDGDISKAS TDAINGSQLY MMSNKLAAYF GGGAGYENGQ WSDPTFMLQQ LNPDGSVVEK NYKTVADAFG SVDSSLSNLN NRLKAVEQGA SPVPPSSDSD VLHWNEEKGG YDASHKGQPG KITEVAEGNI AQGSKDAVNG GQLWETNERL SGVEKDVQHI SDRVDNISNT IADIGDIVNN MADGAVSYDR DEHGNKTNKI SLQGGNAGEP VVIDNVADGK IEKGSKEAVN GGQLHDYTEQ QMKVVLDDAK KYTDERIKNI TVDAIDDAVS QAKDYTDMR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgggtg tttccataac agaaggattt gttcgaaatt cgagtggcac tgatggtcct tatccaaaaa ggagttatgc cagtattgtt tttgctggtg atgataatta ctgtggtgta gataatgtta ttggtcgtgg tggcagtact cagggctcta ataatcaaat aacggcagag gagcaatata acagatttat cgaagataga acatttaatg gccgcaaccc ttatagcaca accactgaaa aagttacttg ggttaaagat ggagtcttga gtaacaacag tggtagttat atgggaacag ccactggtgg tcttcagagt gctctacctg aggcttatgg tgtttattct tttgctaccg gttgtggatt ttctgcgacg ggaaactatt caacagcgtt tggtgcagaa gcgacgggaa aggctggtgg cgcgcaagct tttagtgttg gcgatactga gaatgatata acacgacaaa ttacaggagt ggcagcaggg acctctggta ccgatgcaat gaatgtggca ccgctaaaag ctatgaaaga tttggtagta ggagcttgga aacttgttgt taacgatgaa aatcctatag atgttaatgc aggcagtaca gtaaaatttg taggtgttaa ggccgaagag ggtaatgaag atagtaaaaa tatcaaaatt accacaggaa ataataatga ggtaaaattt gatctaaatg atattatcag agttaaacgt gtaatagcag gcaaggcgaa cgtaagtgaa gtgggttttg tgattactgg tggcccaaac atgactgttg gtggtattaa tgccggaaat aaaaaaatta caggagtagc aaatggtatt cgagagaatg atgcagtaaa tgtttcacag ttgaacgaac taaaaaatca aatagcagga ggtagtcttg ttaattggtt ggaagggcag aagcttatta ctgttggtgc tgaaaaaggt ggcacggaaa ttaatattgc aggcagtgaa ggaaatcgga ctatcgccgg tgtgaaagca gcagaaaaag ataatgaagc tgttaataaa aaacagcttg atgaccatat tgcagatatt actgatatta ttaacaatat taagacagaa agtgcttttg cggttctcta tgataaaaat ggtgataaca ccgttaatta taatagtgtg actttgggcg ggggtaagag cgatggaccc gttgctctcc ttaatgtcaa agatggcaaa attaccaaag aatcgcatga tgcaatcaat ggtagccaaa ttaataagat atctggagat attgcaaaat tttttggcgg tgatgcggca tttgaaggtg gtgtctttgc gggccctaga tataatttat ctgaagtttt tgcagatggt aatgtaaaac aaaaggtctt tgaagatgtt ggctcagcgt tgacagggct tgatatgaat gtcagaagtg tgaataaccg tctaacatat gtagccaata gcgttgatca gcaaattgaa aatttttcta aaaatgcttt aatatggagc gatactgaga atgcctttgt tgcgcttcat acaaacgacg gaaacaaaac ggagagtaag attacctatc ttttggatgg tgacattagt aaagcttcaa cagatgccat aaatggttct cagctttata tgatgagcaa taagcttgct gcttattttg gtggcggtgc tggatatgag aatgggcagt ggtcggatcc aacatttatg cttcagcaat tgaatcctga tggtagtgtt gttgagaaga actataagac tgtagcggat gcttttggta gtgttgatag cagtttgtct aatctcaata atcgtttgaa ggctgttgaa caaggggcat ctccggtacc tccgtcttct gattctgatg tgttacattg gaatgaagag aaaggggggt atgatgcgag ccataagggt caacctggta agattacgga agtagctgag ggtaatattg cgcaaggttc gaaagatgca gtcaatggtg gacagctttg ggaaaccaat gagcgtctct ctggtgttga aaaagatgtt cagcatattt cagacagagt ggacaatatt tccaatacga ttgctgatat tggtgatata gttaacaaca tggctgatgg tgctgttagc tatgaccgag atgagcatgg gaataaaacg aataaaatca gtttacaagg tggtaatgct ggtgaacctg tggtgataga taatgttgcg gatggtaaga tagagaaggg ttcgaaagaa gcggtgaatg gtggtcagtt gcatgactat acggaacaac agatgaaggt tgttcttgat gatgcgaaaa aatatacgga tgaacgtatc aagaatatca ctgttgatgc cattgatgat gctgtttctc aagctaaaga ctatacagat atgaggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BaquA.17833.b.B2.GE37344
| HARVESTED ON: | 9/27/2013 |
| SEQUENCED ON: | 10/3/2013 |
| EXPECTED MW: | 88kDa |
| OBSERVED MW: | 88kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 36 |
Validated AA Sequence
MAHHHHHHMA GVSITEGFVR NSSGTDGPYP KRSYASIVFA GDDNYCGVDN VIGRGGSTQG SNNQITAEEQ YNRFIEDRTF NGRNPYSTTT EKVTWVKDGV LSNNSGSYMG TATGGLQSAL PEAYGVYSFA TGCGFSATGN YSTAFGAEAT GKAGGAQAFS VGDTENDITR QITGVAAGTS GTDAMNVAPL KAMKDLVVGA WKLVVNDENP IDVNAGSTVK FVGVKAEEGN EDSKNIKITT GNNNEVKFDL NDIIRVKRVI AGKANVSEVG FVITGGPNMT VGGINAGNKK ITGVANGIRE ND
Validated NT Sequence
atggctcacc accaccacca ccatatggcg ggtgtttcca taacagaagg atttgttcga aattcgagtg gcactgatgg tccttatcca aaaaggagtt atgccagtat tgtttttgct ggtgatgata attactgtgg tgtagataat gttattggtc gtggtggcag tactcagggc tctaataatc aaataacggc agaggagcaa tataacagat ttatcgaaga tagaacattt aatggccgca acccttatag cacaaccact gaaaaagtta cttgggttaa agatggagtc ttgagtaaca acagtggtag ttatatggga acagccactg gtggtcttca gagtgctcta cctgaggctt atggtgttta ttcttttgct accggttgtg gattttctgc gacgggaaac tattcaacag cgtttggtgc agaagcgacg ggaaaggctg gtggcgcgca agcttttagt gttggcgata ctgagaatga tataacacga caaattacag gagtggcagc agggacctct ggtaccgatg caatgaatgt ggcaccgcta aaagctatga aagatttggt agtaggagct tggaaacttg ttgttaacga tgaaaatcct atagatgtta atgcaggcag tacagtaaaa tttgtaggtg ttaaggccga agagggtaat gaagatagta aaaatatcaa aattaccaca ggaaataata atgaggtaaa atttgatcta aatgatatta tcagagttaa acgtgtaata gcaggcaagg cgaacgtaag tgaagtgggt tttgtgatta ctggtggccc aaacatgact gttggtggta ttaatgccgg aaataaaaaa attacaggag tagcaaatgg tattcgagag aatgatgc
Expected Protein Sequence
MAHHHHHHAG VSITEGFVRN SSGTDGPYPK RSYASIVFAG DDNYCGVDNV IGRGGSTQGS NNQITAEEQY NRFIEDRTFN GRNPYSTTTE KVTWVKDGVL SNNSGSYMGT ATGGLQSALP EAYGVYSFAT GCGFSATGNY STAFGAEATG KAGGAQAFSV GDTENDITRQ ITGVAAGTSG TDAMNVAPLK AMKDLVVGAW KLVVNDENPI DVNAGSTVKF VGVKAEEGNE DSKNIKITTG NNNEVKFDLN DIIRVKRVIA GKANVSEVGF VITGGPNMTV GGINAGNKKI TGVANGIREN DAVNVSQLNE LKNQIAGGSL VNWLEGQKLI TVGAEKGGTE INIAGSEGNR TIAGVKAAEK DNEAVNKKQL DDHIADITDI INNIKTESAF AVLYDKNGDN TVNYNSVTLG GGKSDGPVAL LNVKDGKITK ESHDAINGSQ INKISGDIAK FFGGDAAFEG GVFAGPRYNL SEVFADGNVK QKVFEDVGSA LTGLDMNVRS VNNRLTYVAN SVDQQIENFS KNALIWSDTE NAFVALHTND GNKTESKITY LLDGDISKAS TDAINGSQLY MMSNKLAAYF GGGAGYENGQ WSDPTFMLQQ LNPDGSVVEK NYKTVADAFG SVDSSLSNLN NRLKAVEQGA SPVPPSSDSD VLHWNEEKGG YDASHKGQPG KITEVAEGNI AQGSKDAVNG GQLWETNERL SGVEKDVQHI SDRVDNISNT IADIGDIVNN MADGAVSYDR DEHGNKTNKI SLQGGNAGEP VVIDNVADGK IEKGSKEAVN GGQLHDYTEQ QMKVVLDDAK KYTDERIKNI TVDAIDDAVS QAKDYTDMR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgggtg tttccataac agaaggattt gttcgaaatt cgagtggcac tgatggtcct tatccaaaaa ggagttatgc cagtattgtt tttgctggtg atgataatta ctgtggtgta gataatgtta ttggtcgtgg tggcagtact cagggctcta ataatcaaat aacggcagag gagcaatata acagatttat cgaagataga acatttaatg gccgcaaccc ttatagcaca accactgaaa aagttacttg ggttaaagat ggagtcttga gtaacaacag tggtagttat atgggaacag ccactggtgg tcttcagagt gctctacctg aggcttatgg tgtttattct tttgctaccg gttgtggatt ttctgcgacg ggaaactatt caacagcgtt tggtgcagaa gcgacgggaa aggctggtgg cgcgcaagct tttagtgttg gcgatactga gaatgatata acacgacaaa ttacaggagt ggcagcaggg acctctggta ccgatgcaat gaatgtggca ccgctaaaag ctatgaaaga tttggtagta ggagcttgga aacttgttgt taacgatgaa aatcctatag atgttaatgc aggcagtaca gtaaaatttg taggtgttaa ggccgaagag ggtaatgaag atagtaaaaa tatcaaaatt accacaggaa ataataatga ggtaaaattt gatctaaatg atattatcag agttaaacgt gtaatagcag gcaaggcgaa cgtaagtgaa gtgggttttg tgattactgg tggcccaaac atgactgttg gtggtattaa tgccggaaat aaaaaaatta caggagtagc aaatggtatt cgagagaatg atgcagtaaa tgtttcacag ttgaacgaac taaaaaatca aatagcagga ggtagtcttg ttaattggtt ggaagggcag aagcttatta ctgttggtgc tgaaaaaggt ggcacggaaa ttaatattgc aggcagtgaa ggaaatcgga ctatcgccgg tgtgaaagca gcagaaaaag ataatgaagc tgttaataaa aaacagcttg atgaccatat tgcagatatt actgatatta ttaacaatat taagacagaa agtgcttttg cggttctcta tgataaaaat ggtgataaca ccgttaatta taatagtgtg actttgggcg ggggtaagag cgatggaccc gttgctctcc ttaatgtcaa agatggcaaa attaccaaag aatcgcatga tgcaatcaat ggtagccaaa ttaataagat atctggagat attgcaaaat tttttggcgg tgatgcggca tttgaaggtg gtgtctttgc gggccctaga tataatttat ctgaagtttt tgcagatggt aatgtaaaac aaaaggtctt tgaagatgtt ggctcagcgt tgacagggct tgatatgaat gtcagaagtg tgaataaccg tctaacatat gtagccaata gcgttgatca gcaaattgaa aatttttcta aaaatgcttt aatatggagc gatactgaga atgcctttgt tgcgcttcat acaaacgacg gaaacaaaac ggagagtaag attacctatc ttttggatgg tgacattagt aaagcttcaa cagatgccat aaatggttct cagctttata tgatgagcaa taagcttgct gcttattttg gtggcggtgc tggatatgag aatgggcagt ggtcggatcc aacatttatg cttcagcaat tgaatcctga tggtagtgtt gttgagaaga actataagac tgtagcggat gcttttggta gtgttgatag cagtttgtct aatctcaata atcgtttgaa ggctgttgaa caaggggcat ctccggtacc tccgtcttct gattctgatg tgttacattg gaatgaagag aaaggggggt atgatgcgag ccataagggt caacctggta agattacgga agtagctgag ggtaatattg cgcaaggttc gaaagatgca gtcaatggtg gacagctttg ggaaaccaat gagcgtctct ctggtgttga aaaagatgtt cagcatattt cagacagagt ggacaatatt tccaatacga ttgctgatat tggtgatata gttaacaaca tggctgatgg tgctgttagc tatgaccgag atgagcatgg gaataaaacg aataaaatca gtttacaagg tggtaatgct ggtgaacctg tggtgataga taatgttgcg gatggtaaga tagagaaggg ttcgaaagaa gcggtgaatg gtggtcagtt gcatgactat acggaacaac agatgaaggt tgttcttgat gatgcgaaaa aatatacgga tgaacgtatc aagaatatca ctgttgatgc cattgatgat gctgtttctc aagctaaaga ctatacagat atgaggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BaquA.17833.b.B4.GE36659
| HARVESTED ON: | 2/27/2013 |
| SEQUENCED ON: | 2/2/2013 |
| EXPECTED MW: | 15kDa |
| OBSERVED MW: | 13kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMK AMKDLVVGAW KLVVNDENPI DVNAGSTVKF VGVKAEEGNE DSKNIKITTG NNNEVKFDLN DIIRVKRVIA GKANVSEVGF VITGGPNMTV GGINAGNKKI TGVANGIREN DAVNVSQLNE LKNQIA
Validated NT Sequence
atcttactca cttatgctat ttgatttttt agttcgttca actgtgaaac atttactgca tcattctctc gaataccatt tgctactcct gtaatttttt tatttccggc attaatacca ccaacagtca tgtttgggcc accagtaatc acaaaaccca cttcacttac gttcgccttg cctgctatta cacgtttaac tctgataata tcatttagat caaattttac ctcattatta tttcctgtgg taattttgat atttttacta tcttcattac cctcttcggc cttaacacct acaaatttta ctgtactgcc tgcattaaca tctataggat tttcatcgtt aacaacaagt ttccaagctc ctactaccaa atctttcata gctttcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHKA MKDLVVGAWK LVVNDENPID VNAGSTVKFV GVKAEEGNED SKNIKITTGN NNEVKFDLND IIRVKRVIAG KANVSEVGFV ITGGPNMTVG GINAGNKKIT GVANGIREND AVNVSQLNEL KNQIA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaagcta tgaaagattt ggtagtagga gcttggaaac ttgttgttaa cgatgaaaat cctatagatg ttaatgcagg cagtacagta aaatttgtag gtgttaaggc cgaagagggt aatgaagata gtaaaaatat caaaattacc acaggaaata ataatgaggt aaaatttgat ctaaatgata ttatcagagt taaacgtgta atagcaggca aggcgaacgt aagtgaagtg ggttttgtga ttactggtgg cccaaacatg actgttggtg gtattaatgc cggaaataaa aaaattacag gagtagcaaa tggtattcga gagaatgatg cagtaaatgt ttcacagttg aacgaactaa aaaatcaaat agcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BaquA.17833.b.B9.GE36836
| HARVESTED ON: | 5/1/2013 |
| SEQUENCED ON: | 5/8/2013 |
| EXPECTED MW: | 11kDa |
| OBSERVED MW: | 11kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG QLWETNERLS GVEKDVQHIS DRVDNISNTI ADIGDIVNNM ADGAVSYDRD EHGNKTNKIS LQGGNAGEPV VIDNVADGKI EKGSKEAVNG GQL
Validated NT Sequence
atcttactca cttacaactg accaccattc accgcttctt tcgaaccctt ctctatctta ccatccgcaa cattatctat caccacaggt tcaccagcat taccaccttg taaactgatt ttattcgttt tattcccatg ctcatctcgg tcatagctaa cagcaccatc agccatgttg ttaactatat caccaatatc agcaatcgta ttggaaatat tgtccactct gtctgaaata tgctgaacat ctttttcaac accagagaga cgctcattgg tttcccaaag ctgtcccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHGQ LWETNERLSG VEKDVQHISD RVDNISNTIA DIGDIVNNMA DGAVSYDRDE HGNKTNKISL QGGNAGEPVV IDNVADGKIE KGSKEAVNGG QL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggacagc tttgggaaac caatgagcgt ctctctggtg ttgaaaaaga tgttcagcat atttcagaca gagtggacaa tatttccaat acgattgctg atattggtga tatagttaac aacatggctg atggtgctgt tagctatgac cgagatgagc atgggaataa aacgaataaa atcagtttac aaggtggtaa tgctggtgaa cctgtggtga tagataatgt tgcggatggt aagatagaga agggttcgaa agaagcggtg aatggtggtc agttgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BaquA.17833.b.B4.PW37282
| PURIFICATION DATe: | 4/29/2013 |
| CONCENTRATION: | 28.1mg/ml |
| OBSERVED MW: | 14kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK AMKDLVVGAW KLVVNDENPI DVNAGSTVKF VGVKAEEGNE DSKNIKITTG NNNEVKFDLN DIIRVKRVIA GKANVSEVGF VITGGPNMTV GGINAGNKKI TGVANGIREN DAVNVSQLNE LKNQIA
Validated NT Sequence
atcttactca cttatgctat ttgatttttt agttcgttca actgtgaaac atttactgca tcattctctc gaataccatt tgctactcct gtaatttttt tatttccggc attaatacca ccaacagtca tgtttgggcc accagtaatc acaaaaccca cttcacttac gttcgccttg cctgctatta cacgtttaac tctgataata tcatttagat caaattttac ctcattatta tttcctgtgg taattttgat atttttacta tcttcattac cctcttcggc cttaacacct acaaatttta ctgtactgcc tgcattaaca tctataggat tttcatcgtt aacaacaagt ttccaagctc ctactaccaa atctttcata gctttcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHKA MKDLVVGAWK LVVNDENPID VNAGSTVKFV GVKAEEGNED SKNIKITTGN NNEVKFDLND IIRVKRVIAG KANVSEVGFV ITGGPNMTVG GINAGNKKIT GVANGIREND AVNVSQLNEL KNQIA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAAAGCTA TGAAAGATTT GGTAGTAGGA GCTTGGAAAC TTGTTGTTAA CGATGAAAAT CCTATAGATG TTAATGCAGG CAGTACAGTA AAATTTGTAG GTGTTAAGGC CGAAGAGGGT AATGAAGATA GTAAAAATAT CAAAATTACC ACAGGAAATA ATAATGAGGT AAAATTTGAT CTAAATGATA TTATCAGAGT TAAACGTGTA ATAGCAGGCA AGGCGAACGT AAGTGAAGTG GGTTTTGTGA TTACTGGTGG CCCAAACATG ACTGTTGGTG GTATTAATGC CGGAAATAAA AAAATTACAG GAGTAGCAAA TGGTATTCGA GAGAATGATG CAGTAAATGT TTCACAGTTG AACGAACTAA AAAATCAAAT AGCATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for BaquA.17833.b.B9.PW37313
| PURIFICATION DATe: | 6/17/2013 |
| CONCENTRATION: | 25mg/ml |
| OBSERVED MW: | 12kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG QLWETNERLS GVEKDVQHIS DRVDNISNTI ADIGDIVNNM ADGAVSYDRD EHGNKTNKIS LQGGNAGEPV VIDNVADGKI EKGSKEAVNG GQL
Validated NT Sequence
atcttactca cttacaactg accaccattc accgcttctt tcgaaccctt ctctatctta ccatccgcaa cattatctat caccacaggt tcaccagcat taccaccttg taaactgatt ttattcgttt tattcccatg ctcatctcgg tcatagctaa cagcaccatc agccatgttg ttaactatat caccaatatc agcaatcgta ttggaaatat tgtccactct gtctgaaata tgctgaacat ctttttcaac accagagaga cgctcattgg tttcccaaag ctgtcccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHGQ LWETNERLSG VEKDVQHISD RVDNISNTIA DIGDIVNNMA DGAVSYDRDE HGNKTNKISL QGGNAGEPVV IDNVADGKIE KGSKEAVNGG QL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGACAGC TTTGGGAAAC CAATGAGCGT CTCTCTGGTG TTGAAAAAGA TGTTCAGCAT ATTTCAGACA GAGTGGACAA TATTTCCAAT ACGATTGCTG ATATTGGTGA TATAGTTAAC AACATGGCTG ATGGTGCTGT TAGCTATGAC CGAGATGAGC ATGGGAATAA AACGAATAAA ATCAGTTTAC AAGGTGGTAA TGCTGGTGAA CCTGTGGTGA TAGATAATGT TGCGGATGGT AAGATAGAGA AGGGTTCGAA AGAAGCGGTG AATGGTGGTC AGTTGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT