BaquA.17833.a

VompC

CENTER ID: BaquA.17833.a
ORGANISM: Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS: NMR assigned
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.17833.a.B11.GE36915 VompC:aa253-511 253 511
BaquA.17833.a.B12.GE36916 VompC:aa253-634 253 634
BaquA.17833.a.B15.GE37167 VompC:aa134-326 (MV:173-244) 134 326
BaquA.17833.a.B2.GE36654 mature protein, soluble domain 49 880
BaquA.17833.a.B4.GE36656 VompC:aa319-385 319 385
BaquA.17833.a.B5.GE36917 VompC:aa400-511 400 511
BaquA.17833.a.B7.GE37088 VompC:aa635-758 635 758
BaquA.17833.a.B8.GE36832 VompC:aa760-835 760 835
BaquA.17833.a.B9.GE36833 VompC:aa60-394 (MV:173-244) 60 394
BaquA.17833.a.MB15.GE38118 VompC:aa134-326 (MV:173-244) 134 326
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
UniProt: Q64HS7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGILQFLLGN FMRELHSRLS FFKAGSLSIA LVILLSSVSA VFASTLPMTQ EVILDRKTKN DRKTKNDRKT ICILSLSVFD GNVVLAEDDD DYVCGLDQVS GFNVLDINPV EESLEKEGIS LTIKREDSAL KRLRALSGVF GVQSIRSGSH PYASGYEAAI FDLGETKKVA GPQFYGHLEY GKSWINIQWA DRACKVNSCY KLWRAPELSV SIGTGSGYGS SFAAVSVGVS AGVAPDLSTW VGAEQSVALG SGSRTRPREH ARGWDPRING SNSNDNNPWW VSSWGEVSIG NVDLHKTRHI AGVAAGWEDT DAVNVAQLKA LREWAEGRPW LAYYDEGSKV IRIGSKRSGD KIDIKNSDGQ DRVLAGVKDG EVSKGSVDAI NGSQLWKTNQ TVAGLNVTLN DLGKNLTNYL GGGADVAQNV APTYIIEDKK YRDVGSAFSG VDTVIKDIYS KLEDLPDGDV KDQDALMWSD TENAFVALHT KDGNKTNSKI TYLLDGDISK ASTDAINGSQ LYMMSNKLAA YFGGGAGYEN GQWSDPIFTL HQFNPDGNFV EKNYKTVADA FGGVDTVIKD IYSKLGDLPG GGVKDQDALM WSETENAFVA LHGLEGKKTN SKLKFLLDGA IEQGSSEAIT GNQLYMMSNK LAAYFGGGAR YENGQWLDPT FMLHQLNPDG SVVEKSYKTV SDAFGGVDSS LSNLNDRLKA VEQGASPVPP SSDSDVLHWN EGKGGYDASH KGQPGKITEV AEGNIAQGSK DAVNGGQLWE TNERLSGVEQ KVDAIEQGIG QVVDGVVSYD RDEHGNKTNK ISLQGGNAGE PVVIDNVADG KIEKGSKEAV NGGQLHDYTE QQMKVVLDDA KKYTDERIKN ITVDAINDAV SQAKDYTDMK FEALNYGIEG ARKEARQAAA IGLAVSNLRY NDTPGKLSIA FGGGLWRSQG AFAFGAGYAS EDGKILSNGS ITTSSGHWGI GAGLSLKLKS
NT Sequence
atgggaattt tgcaattttt attggggaat tttatgaggg aattgcattc aagactaagt ttttttaaag cgggttcatt aagcatcgct ctggttatac tcttatcgag tgtatcggct gtttttgcct cgactttacc tatgacccag gaagttattt tggatcgcaa gactaagaat gatcgtaaga ctaagaatga tcgtaagact atatgtattc tttctctaag tgtttttgat ggaaatgttg ttttagcaga agatgatgat gattatgttt gtggtttaga tcaggtttct gggtttaatg tgttagatat caacccagtt gaggagagtt tagagaaaga gggtatttca cttacgataa agagagagga tagcgctttg aagaggctaa gagctctctc tggagttttt ggtgttcagt ctattaggag cggttcccat ccctatgcat caggttatga ggcagcaatc tttgatctgg gtgaaacaaa aaaagttgca ggaccacaat tctacgggca tctggagtat ggtaaatcat ggatcaatat acaatgggca gacagggctt gtaaggtaaa ctcttgttat aagttatgga gggcacctga gctgtcggtt tcaataggaa ctggttccgg ttacggcagc agcttcgcag ctgtttctgt gggtgtcagc gcaggggttg cccccgatct ctcgacttgg gttggtgctg agcagagtgt agctctaggt tctggttctc ggactagacc gagagagcac gccaggggct gggatcctcg aataaatggt tctaattcca atgataataa tccgtggtgg gtgtcgtctt ggggtgaggt tagcatcggt aacgttgatc tccataaaac ccgacatata gcaggagtgg cagcaggctg ggaagacacc gatgcagtca atgtagcaca actaaaagcg ttaagagagt gggcagaagg tcgcccatgg ctggcttatt atgatgaagg ttctaaggtt attcgcattg gttcaaagcg ctcgggagat aaaatagaca ttaaaaatag cgatgggcaa gatcgagttt tggctggagt gaaggacggg gaggtttcta aggggtcagt tgacgcaata aatggcagtc aactttggaa aaccaatcaa acagttgcgg gtctaaatgt aactttaaat gatctaggta agaatctcac aaactattta ggtggtggcg cagatgtggc tcagaatgtt gcaccaactt atattattga ggacaagaag tatcgtgatg ttggttctgc ttttagtggt gttgatacgg tcatcaagga tatttatagt aaacttgagg atttgcctga tggagacgtt aaggatcaag atgccttaat gtggagtgat actgagaatg cttttgttgc acttcacaca aaagacggaa acaaaacgaa cagtaagatt acctatcttt tggatggtga cattagtaaa gcttcaacag atgccataaa tggttctcag ctttatatga tgagcaataa gcttgctgct tattttggtg gcggtgctgg atatgagaat gggcagtggt cggaccctat atttacgctt caccaattca atcctgatgg caattttgtt gagaagaact ataagactgt agcggatgct tttggtggtg ttgatacggt tatcaaggat atttatagta aacttgggga tttgcctggt ggaggcgtta aggatcaaga tgccttaatg tggagcgaga ctgagaatgc ttttgttgcg cttcatggat tggaaggaaa gaagacgaat agcaaactga agtttctctt agatggtgcc attgagcaag ggtcgagtga agcgattacg ggtaatcagc tttatatgat gagcaataag cttgctgctt attttggtgg cggtgctaga tatgagaatg ggcagtggtt ggaccctaca tttatgcttc accaattgaa tcctgatggt agtgttgttg aaaagagcta taagactgta tcggatgctt ttggtggtgt tgatagcagt ttgtctaatc tcaatgatcg tttgaaggct gttgaacaag gggcatctcc ggtacctccg tcttctgatt ctgatgtgtt acattggaat gaagggaagg gggggtatga tgcgagccat aagggtcaac ctggtaagat tacggaagta gctgagggta atattgcgca aggttcgaaa gatgcagtca atggtggaca gctttgggaa accaatgagc gtctctctgg tgttgaacaa aaggttgatg ctattgagca agggattggt caggttgttg atggtgttgt tagctatgac cgagatgagc atgggaataa aacgaataaa atcagtttac aaggtggtaa tgctggtgaa cctgtggtga tagataatgt tgcggatggg aagatagaga agggttcgaa agaagcggtg aatggtggtc agttgcatga ctatacggaa caacagatga aggttgttct tgatgatgcg aaaaaatata cggatgaacg tatcaagaat atcactgttg atgccattaa tgatgctgtt tctcaagcta aagactatac agatatgaag tttgaggctt taaattatgg cattgagggt gctcggaaag aagcgaggca agcagcggct ataggtttag cggtatctaa cttacgttac aatgatacac ctggaaaatt aagcattgca tttgggggtg ggctatggcg tagtcaaggt gcatttgcct ttggtgcggg ttatgcgtct gaagatggta agatcctttc taatgggtcg ataacgactt ctagtggtca ttggggaata ggtgcggggc taagcttaaa actaaaatca tga
Details for BaquA.17833.a.B11.GE36915
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS RTRPREHARG WDPRINGSNS NDNNPWWVSS WGEVSIGNVD LHKTRHIAGV AAGWEDTDAV NVAQLKALRE WAEGRPWLAY YDEGSKVIRI GSKRSGDKID IKNSDGQDRV LAGVKDGEVS KGSVDAINGS QLWKTNQTVA GLNVTLNDLG KNLTNYLGGG ADVAQNVAPT YIIEDKKYRD VGSAFSGVDT VIKDIYSKLE DLPDGDVKDQ DALMWSDTEN AFVALHTKDG NKTNSKITYL LDGDISKAST DAINGSQL
Validated NT Sequence
cagccggntc ctatcttact cacttaaagc tgagaaccat ttatggcatc tgttgaagct ttactaatgt caccatccaa aagataggta atcttactgt tcgttttgtt tccgtctttt gtgtgaagtg caacaaaagc attctcagta tcactccaca ttaaggcatc ttgatcctta acgtctccat caggcaaatc ctcaagttta ctataaatat ccttgatgac cgtatcaaca ccactaaaag cagaaccaac atcacgatac ttcttgtcct caataatata agttggtgca acattctgag ccacatctgc gccaccacct aaatagtttg tgagattctt acctagatca tttaaagtta catttagacc cgcaactgtt tgattggttt tccaaagttg actgccattt attgcgtcaa ctgacccctt agaaacctcc ccgtccttca ctccagccaa aactcgatct tgcccatcgc tatttttaat gtctatttta tctcccgagc gctttgaacc aatgcgaata accttagaac cttcatcata ataagccagc catgggcgac cttctgccca ctctcttaac gcttttagtt gtgctacatt gactgcatcg gtgtcttccc agcctgctgc cactcctgct atatgtcggg ttttatggag atcaacgtta ccgatgctaa cctcacccca agacgacacc caccacggat tattatcatt ggaattagaa ccatttattc gaggatccca gcccctggcg tgctctctcg gtctagtccg agacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHSR TRPREHARGW DPRINGSNSN DNNPWWVSSW GEVSIGNVDL HKTRHIAGVA AGWEDTDAVN VAQLKALREW AEGRPWLAYY DEGSKVIRIG SKRSGDKIDI KNSDGQDRVL AGVKDGEVSK GSVDAINGSQ LWKTNQTVAG LNVTLNDLGK NLTNYLGGGA DVAQNVAPTY IIEDKKYRDV GSAFSGVDTV IKDIYSKLED LPDGDVKDQD ALMWSDTENA FVALHTKDGN KTNSKITYLL DGDISKASTD AINGSQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctcgga ctagaccgag agagcacgcc aggggctggg atcctcgaat aaatggttct aattccaatg ataataatcc gtggtgggtg tcgtcttggg gtgaggttag catcggtaac gttgatctcc ataaaacccg acatatagca ggagtggcag caggctggga agacaccgat gcagtcaatg tagcacaact aaaagcgtta agagagtggg cagaaggtcg cccatggctg gcttattatg atgaaggttc taaggttatt cgcattggtt caaagcgctc gggagataaa atagacatta aaaatagcga tgggcaagat cgagttttgg ctggagtgaa ggacggggag gtttctaagg ggtcagttga cgcaataaat ggcagtcaac tttggaaaac caatcaaaca gttgcgggtc taaatgtaac tttaaatgat ctaggtaaga atctcacaaa ctatttaggt ggtggcgcag atgtggctca gaatgttgca ccaacttata ttattgagga caagaagtat cgtgatgttg gttctgcttt tagtggtgtt gatacggtca tcaaggatat ttatagtaaa cttgaggatt tgcctgatgg agacgttaag gatcaagatg ccttaatgtg gagtgatact gagaatgctt ttgttgcact tcacacaaaa gacggaaaca aaacgaacag taagattacc tatcttttgg atggtgacat tagtaaagct tcaacagatg ccataaatgg ttctcagctt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BaquA.17833.a.B12.GE36916
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 40
Validated AA Sequence
MWSDTENAFV ALHTKDGNKT NSKITYLLDG DISKASTDAI NGSQLYMMSN KLAAYFGGGA GYENGQWSDP IFTLHQFNPD GNFVEKNYKT VADAFGGVDT VIKDIYSKLG DLPGGGVKDQ DALMWSETEN AFVALHGLEG KKTNSKLKFL LDGAIEQG
Validated NT Sequence
gacccttgct caatggcacc atctaagaga aacttcagtt tgctattcgt cttctttcct tccaatccat gaagcgcaac aaaagcattc tcagtctcgc tccacattaa ggcatcttga tccttaacgc ctccaccagg caaatcccca agtttactat aaatatcctt gataaccgta tcaacaccac caaaagcatc cgctacagtc ttatagttct tctcaacaaa attgccatca ggattgaatt ggtgaagcgt aaatataggg tccgaccact gcccattctc atatccagca ccgccaccaa aataagcagc aagcttattg ctcatcatat aaagctgaga accatttatg gcatctgttg aagctttact aatgtcacca tccaaaagat aggtaatctt actgttcgtt ttgtttccgt cttttgtgtg aagtgcaaca aaagcattct cagtatcact ccacattaag gcatcttgat ccttaacgtc tccatcaggc aaatcctcaa gtttactata aatatccttg atgaccgtat caacaccact aaaagcagaa ccaacatcac gatacttctt gtcctcaata atataagttg gtgcaacatt ctgagccaca tctgcgccac cacctaaata gtttgtgaga ttcttaccta gatcatttaa agttacattt agacccgcaa ctgtttgatt ggttttccaa agttgactgc catttattgc gtcaactgac cccttagaaa cctccccgtc cttcactcca gccaaaactc gatcttgccc atcgctattt ttaatgtcta ttttatctcc cgagcgcttt gaaccaatgc gaataacctt agaaccttca tcataataag ccagccatgg gcgaccttct gcccactctc ttaacgcttt tagttgtgct acattgactg catcggtgtc ttcccagcct gctgccactc ctgctatatg tcgggtttta tggagatcaa cgttaccgat gctaacctca ccccaagacg acacccacca cggattatta tcattggaat tagaaccatt tattcgagga tcccagcccc tggcgtgctc tctcggtcta gtcc
Expected Protein Sequence
MAHHHHHHSR TRPREHARGW DPRINGSNSN DNNPWWVSSW GEVSIGNVDL HKTRHIAGVA AGWEDTDAVN VAQLKALREW AEGRPWLAYY DEGSKVIRIG SKRSGDKIDI KNSDGQDRVL AGVKDGEVSK GSVDAINGSQ LWKTNQTVAG LNVTLNDLGK NLTNYLGGGA DVAQNVAPTY IIEDKKYRDV GSAFSGVDTV IKDIYSKLED LPDGDVKDQD ALMWSDTENA FVALHTKDGN KTNSKITYLL DGDISKASTD AINGSQLYMM SNKLAAYFGG GAGYENGQWS DPIFTLHQFN PDGNFVEKNY KTVADAFGGV DTVIKDIYSK LGDLPGGGVK DQDALMWSET ENAFVALHGL EGKKTNSKLK FLLDGAIEQG SSEAITGNQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctcgga ctagaccgag agagcacgcc aggggctggg atcctcgaat aaatggttct aattccaatg ataataatcc gtggtgggtg tcgtcttggg gtgaggttag catcggtaac gttgatctcc ataaaacccg acatatagca ggagtggcag caggctggga agacaccgat gcagtcaatg tagcacaact aaaagcgtta agagagtggg cagaaggtcg cccatggctg gcttattatg atgaaggttc taaggttatt cgcattggtt caaagcgctc gggagataaa atagacatta aaaatagcga tgggcaagat cgagttttgg ctggagtgaa ggacggggag gtttctaagg ggtcagttga cgcaataaat ggcagtcaac tttggaaaac caatcaaaca gttgcgggtc taaatgtaac tttaaatgat ctaggtaaga atctcacaaa ctatttaggt ggtggcgcag atgtggctca gaatgttgca ccaacttata ttattgagga caagaagtat cgtgatgttg gttctgcttt tagtggtgtt gatacggtca tcaaggatat ttatagtaaa cttgaggatt tgcctgatgg agacgttaag gatcaagatg ccttaatgtg gagtgatact gagaatgctt ttgttgcact tcacacaaaa gacggaaaca aaacgaacag taagattacc tatcttttgg atggtgacat tagtaaagct tcaacagatg ccataaatgg ttctcagctt tatatgatga gcaataagct tgctgcttat tttggtggcg gtgctggata tgagaatggg cagtggtcgg accctatatt tacgcttcac caattcaatc ctgatggcaa ttttgttgag aagaactata agactgtagc ggatgctttt ggtggtgttg atacggttat caaggatatt tatagtaaac ttggggattt gcctggtgga ggcgttaagg atcaagatgc cttaatgtgg agcgagactg agaatgcttt tgttgcgctt catggattgg aaggaaagaa gacgaatagc aaactgaagt ttctcttaga tggtgccatt gagcaagggt cgagtgaagc gattacgggt aatcagcttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaquA.17833.a.B15.GE37167
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR ALSGVFGVQS IRSGSHPYAS GYEAAIFDLG ETKKVAGPQF YGHLEYGKSW INIQWADRAC KVNSCYKLWR APELSVSIGT GSGYGSSFAA VSVGVSAGVA PDLSTWVGAE QSVALGSGSR TRPREHARGW DPRINGSNSN DNNPWWVSSW GEVSIGNVDL HKTRHIAGVA AGWEDTDAVN VAQLKALREW AE
Validated NT Sequence
atcttactca cttattctgc ccactctctt aacgctttta gttgtgctac attgactgca tcggtgtctt cccagcctgc tgccactcct gctatatgtc gggttttatg gagatcaacg ttaccgatgc taacctcacc ccaagacgac acccaccacg gattattatc attggaatta gaaccattta ttcgaggatc ccagcccctg gcgtgctctc tcggtctagt ccgagaacca gaacctagag ctacactctg ctcagcacca acccaagtcg agagatcggg ggcaacccct gcgctgacac ccacagaaac agctgcgaag ctgctgccgt aaccggaacc agttcctatt gaaaccgaca gctcaggtgc cctccataac ttataacaag agtttacctt acaagccctg tctgcccatt gtatattgat ccatgattta ccatactcca gatgcccgta gaattgtggt cctgcaactt tttttgtttc acccagatca aagattgctg cctcataacc tgatgcatag ggatgggaac cgctcctaat agactgaaca ccaaaaactc cagagagagc tctcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHRA LSGVFGVQSI RSGSHPYASG YEAAIFDLGE TKKVAGPQFY GHLEYGKSWI NIQWADRACK VNSCYKLWRA PELSVSIGTG SGYGSSFAAV SVGVSAGVAP DLSTWVGAEQ SVALGSGSRT RPREHARGWD PRINGSNSND NNPWWVSSWG EVSIGNVDLH KTRHIAGVAA GWEDTDAVNV AQLKALREWA E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagagctc tctctggagt ttttggtgtt cagtctatta ggagcggttc ccatccctat gcatcaggtt atgaggcagc aatctttgat ctgggtgaaa caaaaaaagt tgcaggacca caattctacg ggcatctgga gtatggtaaa tcatggatca atatacaatg ggcagacagg gcttgtaagg taaactcttg ttataagtta tggagggcac ctgagctgtc ggtttcaata ggaactggtt ccggttacgg cagcagcttc gcagctgttt ctgtgggtgt cagcgcaggg gttgcccccg atctctcgac ttgggttggt gctgagcaga gtgtagctct aggttctggt tctcggacta gaccgagaga gcacgccagg ggctgggatc ctcgaataaa tggttctaat tccaatgata ataatccgtg gtgggtgtcg tcttggggtg aggttagcat cggtaacgtt gatctccata aaacccgaca tatagcagga gtggcagcag gctgggaaga caccgatgca gtcaatgtag cacaactaaa agcgttaaga gagtgggcag aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BaquA.17833.a.B2.GE36654
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 92kDa
OBSERVED MW: 92kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 32
Validated AA Sequence
MAHHHHHHMT QEVILDRKTK NDRKTKNDRK TICILSLSVF DGNVVLAEDD DDYVCGLDQV SGFNVLDINP VEESLEKEGI SLTIKREDSA LKRLRALSGV FGVQSIRSGS HPYASGYEAA IFDLGETKKV AGPQFYGHLE YGKSWINIQW ADRACKVNSC YKLWRAPELS VSIGTGSGYG SSFAAVSVGV SAGVAPDLST WVGAEQSVAL GSGSRTRPRE HARGWDPRIN GSNSNDNNPW WVSSWGEXSI GNVDLHKTRH IAXWQQAGKT PMQSM
Validated NT Sequence
tannnnnnnn nnnttnnctt taagaaggag nnataccatg gctcaccacc accaccacca tatgacccag gaagttattt tggatcgcaa gactaagaat gatcgtaaga ctaagaatga tcgtaagact atatgtattc tttctctaag tgtttttgat ggaaatgttg ttttagcaga agatgatgat gattatgttt gtggtttaga tcaggtttct gggtttaatg tgttagatat caacccagtt gaggagagtt tagagaaaga gggtatttca cttacgataa agagagagga tagcgctttg aagaggctaa gagctctctc tggagttttt ggtgttcagt ctattaggag cggttcccat ccctatgcat caggttatga ggcagcaatc tttgatctgg gtgaaacaaa aaaagttgca ggaccacaat tctacgggca tctggagtat ggtaaatcat ggatcaatat acaatgggca gacagggctt gtaaggtaaa ctcttgttat aagttatgga gggcacctga gctgtcggtt tcaataggaa ctggttccgg ttacggcagc agcttcgcag ctgtttctgt gggtgtcagc gcaggggttg cccccgatct ctcgacttgg gttggtgctg agcagagtgt agctctaggt tctggttctc ggactagacc gagagagcac gccaggggct gggatcctcg aataaatggt tctaattcca atgataataa tccgtggtgg gtgtcgtctt ggggtgaggn tagcatcggt aacgttgatc tccataaaac ccgacatata gcnnngtggc agcaggctgg gaagacaccg atgcagtcaa tgtagcacaa ctaaaagcgt taagagagtg ggcagaannn cgcccatggc tggcttatta tgatgaaggt tctaanntnt tcgcattngt ncaaagcnnt cgggananaa atagacntta aaaatagcga tgggcanatc ganttttngn ngnngtgnnn nacggggang nttctaggnc ant
Expected Protein Sequence
MAHHHHHHTQ EVILDRKTKN DRKTKNDRKT ICILSLSVFD GNVVLAEDDD DYVCGLDQVS GFNVLDINPV EESLEKEGIS LTIKREDSAL KRLRALSGVF GVQSIRSGSH PYASGYEAAI FDLGETKKVA GPQFYGHLEY GKSWINIQWA DRACKVNSCY KLWRAPELSV SIGTGSGYGS SFAAVSVGVS AGVAPDLSTW VGAEQSVALG SGSRTRPREH ARGWDPRING SNSNDNNPWW VSSWGEVSIG NVDLHKTRHI AGVAAGWEDT DAVNVAQLKA LREWAEGRPW LAYYDEGSKV IRIGSKRSGD KIDIKNSDGQ DRVLAGVKDG EVSKGSVDAI NGSQLWKTNQ TVAGLNVTLN DLGKNLTNYL GGGADVAQNV APTYIIEDKK YRDVGSAFSG VDTVIKDIYS KLEDLPDGDV KDQDALMWSD TENAFVALHT KDGNKTNSKI TYLLDGDISK ASTDAINGSQ LYMMSNKLAA YFGGGAGYEN GQWSDPIFTL HQFNPDGNFV EKNYKTVADA FGGVDTVIKD IYSKLGDLPG GGVKDQDALM WSETENAFVA LHGLEGKKTN SKLKFLLDGA IEQGSSEAIT GNQLYMMSNK LAAYFGGGAR YENGQWLDPT FMLHQLNPDG SVVEKSYKTV SDAFGGVDSS LSNLNDRLKA VEQGASPVPP SSDSDVLHWN EGKGGYDASH KGQPGKITEV AEGNIAQGSK DAVNGGQLWE TNERLSGVEQ KVDAIEQGIG QVVDGVVSYD RDEHGNKTNK ISLQGGNAGE PVVIDNVADG KIEKGSKEAV NGGQLHDYTE QQMKVVLDDA KKYTDERIKN ITVDAINDAV SQAKDYTDMK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacccagg aagttatttt ggatcgcaag actaagaatg atcgtaagac taagaatgat cgtaagacta tatgtattct ttctctaagt gtttttgatg gaaatgttgt tttagcagaa gatgatgatg attatgtttg tggtttagat caggtttctg ggtttaatgt gttagatatc aacccagttg aggagagttt agagaaagag ggtatttcac ttacgataaa gagagaggat agcgctttga agaggctaag agctctctct ggagtttttg gtgttcagtc tattaggagc ggttcccatc cctatgcatc aggttatgag gcagcaatct ttgatctggg tgaaacaaaa aaagttgcag gaccacaatt ctacgggcat ctggagtatg gtaaatcatg gatcaatata caatgggcag acagggcttg taaggtaaac tcttgttata agttatggag ggcacctgag ctgtcggttt caataggaac tggttccggt tacggcagca gcttcgcagc tgtttctgtg ggtgtcagcg caggggttgc ccccgatctc tcgacttggg ttggtgctga gcagagtgta gctctaggtt ctggttctcg gactagaccg agagagcacg ccaggggctg ggatcctcga ataaatggtt ctaattccaa tgataataat ccgtggtggg tgtcgtcttg gggtgaggtt agcatcggta acgttgatct ccataaaacc cgacatatag caggagtggc agcaggctgg gaagacaccg atgcagtcaa tgtagcacaa ctaaaagcgt taagagagtg ggcagaaggt cgcccatggc tggcttatta tgatgaaggt tctaaggtta ttcgcattgg ttcaaagcgc tcgggagata aaatagacat taaaaatagc gatgggcaag atcgagtttt ggctggagtg aaggacgggg aggtttctaa ggggtcagtt gacgcaataa atggcagtca actttggaaa accaatcaaa cagttgcggg tctaaatgta actttaaatg atctaggtaa gaatctcaca aactatttag gtggtggcgc agatgtggct cagaatgttg caccaactta tattattgag gacaagaagt atcgtgatgt tggttctgct tttagtggtg ttgatacggt catcaaggat atttatagta aacttgagga tttgcctgat ggagacgtta aggatcaaga tgccttaatg tggagtgata ctgagaatgc ttttgttgca cttcacacaa aagacggaaa caaaacgaac agtaagatta cctatctttt ggatggtgac attagtaaag cttcaacaga tgccataaat ggttctcagc tttatatgat gagcaataag cttgctgctt attttggtgg cggtgctgga tatgagaatg ggcagtggtc ggaccctata tttacgcttc accaattcaa tcctgatggc aattttgttg agaagaacta taagactgta gcggatgctt ttggtggtgt tgatacggtt atcaaggata tttatagtaa acttggggat ttgcctggtg gaggcgttaa ggatcaagat gccttaatgt ggagcgagac tgagaatgct tttgttgcgc ttcatggatt ggaaggaaag aagacgaata gcaaactgaa gtttctctta gatggtgcca ttgagcaagg gtcgagtgaa gcgattacgg gtaatcagct ttatatgatg agcaataagc ttgctgctta ttttggtggc ggtgctagat atgagaatgg gcagtggttg gaccctacat ttatgcttca ccaattgaat cctgatggta gtgttgttga aaagagctat aagactgtat cggatgcttt tggtggtgtt gatagcagtt tgtctaatct caatgatcgt ttgaaggctg ttgaacaagg ggcatctccg gtacctccgt cttctgattc tgatgtgtta cattggaatg aagggaaggg ggggtatgat gcgagccata agggtcaacc tggtaagatt acggaagtag ctgagggtaa tattgcgcaa ggttcgaaag atgcagtcaa tggtggacag ctttgggaaa ccaatgagcg tctctctggt gttgaacaaa aggttgatgc tattgagcaa gggattggtc aggttgttga tggtgttgtt agctatgacc gagatgagca tgggaataaa acgaataaaa tcagtttaca aggtggtaat gctggtgaac ctgtggtgat agataatgtt gcggatggga agatagagaa gggttcgaaa gaagcggtga atggtggtca gttgcatgac tatacggaac aacagatgaa ggttgttctt gatgatgcga aaaaatatac ggatgaacgt atcaagaata tcactgttga tgccattaat gatgctgttt ctcaagctaa agactataca gatatgaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaquA.17833.a.B4.GE36656
HARVESTED ON: 2/27/2013
SEQUENCED ON: 1/26/2017
EXPECTED MW: 9kDa
OBSERVED MW: 9kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMK ALREWAEGRP WLAYYDEGSK VIRIGSKRSG DKIDIKNSDG QDRVLAGVKD GEVSKGSVDA INGSQL
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa gcgttaagag agtgggcaga aggtcgccca tggctggctt attatgatga aggttctaag gttattcgca ttggttcaaa gcgctcggga gataaaatag acattaaaaa tagcgatggg caagatcgag ttttggctgg agtgaaggac ggggaggttt ctaaggggtc agttgacgca ataaatggca gtcaacttta agtgagtaag at
Expected Protein Sequence
MAHHHHHHKA LREWAEGRPW LAYYDEGSKV IRIGSKRSGD KIDIKNSDGQ DRVLAGVKDG EVSKGSVDAI NGSQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaagcgt taagagagtg ggcagaaggt cgcccatggc tggcttatta tgatgaaggt tctaaggtta ttcgcattgg ttcaaagcgc tcgggagata aaatagacat taaaaatagc gatgggcaag atcgagtttt ggctggagtg aaggacgggg aggtttctaa ggggtcagtt gacgcaataa atggcagtca actttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BaquA.17833.a.B5.GE36917
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 13kDa
OBSERVED MW: 13kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN DLGKNLTNYL GGGADVAQNV APTYIIEDKK YRDVGSAFSG VDTVIKDIYS KLEDLPDGDV KDQDALMWSD TENAFVALHT KDGNKTNSKI TYLLDGDISK ASTDAINGSQ L
Validated NT Sequence
ttactcactt aaagctgaga accatttatg gcatctgttg aagctttact aatgtcacca tccaaaagat aggtaatctt actgttcgtt ttgtttccgt cttttgtgtg aagtgcaaca aaagcattct cagtatcact ccacattaag gcatcttgat ccttaacgtc tccatcaggc aaatcctcaa gtttactata aatatccttg atgaccgtat caacaccact aaaagcagaa ccaacatcac gatacttctt gtcctcaata atataagttg gtgcaacatt ctgagccaca tctgcgccac cacctaaata gtttgtgaga ttcttaccta gatcattcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHND LGKNLTNYLG GGADVAQNVA PTYIIEDKKY RDVGSAFSGV DTVIKDIYSK LEDLPDGDVK DQDALMWSDT ENAFVALHTK DGNKTNSKIT YLLDGDISKA STDAINGSQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatgatc taggtaagaa tctcacaaac tatttaggtg gtggcgcaga tgtggctcag aatgttgcac caacttatat tattgaggac aagaagtatc gtgatgttgg ttctgctttt agtggtgttg atacggtcat caaggatatt tatagtaaac ttgaggattt gcctgatgga gacgttaagg atcaagatgc cttaatgtgg agtgatactg agaatgcttt tgttgcactt cacacaaaag acggaaacaa aacgaacagt aagattacct atcttttgga tggtgacatt agtaaagctt caacagatgc cataaatggt tctcagcttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaquA.17833.a.B7.GE37088
HARVESTED ON: 7/17/2013
SEQUENCED ON: 7/20/2013
EXPECTED MW: 14kDa
OBSERVED MW: 15kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHYM MSNKLAAYFG GGARYENGQW LDPTFMLHQL NPDGSVVEKS YKTVSDAFGG VDSSLSNLND RLKAVEQGAS PVPPSSDSDV LHWNEGKGGY DASHKGQPGK ITEVAEGNIA QGSKDAVNGG QL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattatatga tgagcaataa gcttgctgct tattttggtg gcggtgctag atatgagaat gggcagtggt tggaccctac atttatgctt caccaattga atcctgatgg tagtgttgtt gaaaagagct ataagactgt atcggatgct tttggtggtg ttgatagcag tttgtctaat ctcaatgatc gtttgaaggc tgttgaacaa ggggcatctc cggtacctcc gtcttctgat tctgatgtgt tacattggaa tgaagggaag ggggggtatg atgcgagcca taagggtcaa cctggtaaga ttacggaagt agctgagggt aatattgcgc aaggttcgaa agatgcagtc aatggtggac agctttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BaquA.17833.a.B8.GE36832
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 9kDa
OBSERVED MW: 8kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHME TNERLSGVEQ KVDAIEQGIG QVVDGVVSYD RDEHGNKTNK ISLQGGNAGE PVVIDNVADG KIEKGSKEAV NGGQL
Validated NT Sequence
atcttactca cttacaactg accaccattc accgcttctt tcgaaccctt ctctatcttc ccatccgcaa cattatctat caccacaggt tcaccagcat taccaccttg taaactgatt ttattcgttt tattcccatg ctcatctcgg tcatagctaa caacaccatc aacaacctga ccaatccctt gctcaatagc atcaaccttt tgttcaacac cagagagacg ctcattggtt tccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHET NERLSGVEQK VDAIEQGIGQ VVDGVVSYDR DEHGNKTNKI SLQGGNAGEP VVIDNVADGK IEKGSKEAVN GGQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaacca atgagcgtct ctctggtgtt gaacaaaagg ttgatgctat tgagcaaggg attggtcagg ttgttgatgg tgttgttagc tatgaccgag atgagcatgg gaataaaacg aataaaatca gtttacaagg tggtaatgct ggtgaacctg tggtgataga taatgttgcg gatgggaaga tagagaaggg ttcgaaagaa gcggtgaatg gtggtcagtt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BaquA.17833.a.B9.GE36833
HARVESTED ON: 5/1/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 37kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMN DRKTICILSL SVFDGNVVLA EDDDDYVCGL DQVSGFNVLD INPVEESLEK EGISLTIKRE DSALKRLRAL SGVFGVQSIR SGSHPYASGY EAAIFDLGET KKVAGPQFYG HLEYGKSWIN IQWADRACKV NSCYKLWRAP ELSVSIGTGS GYGSSFAAVS VGVSAGVAPD LSTWVGAEQS VALGSGSRTR PREHARGWDP RINGSNSNDN NPWWVSSWGE VSIGNVDLHK TRHIAGVAAG WEDTDAVNVA QLKALREWAE GRPWLAYYDE GSKVIRIGSK RSGDKIDIKN SDGQDRVLAG VKDGEVSKGS VDAINGSQLW
Validated NT Sequence
tccaaagttg actgccattt attgcgtcaa ctgacccctt agaaacctcc ccgtccttca ctccagccaa aactcgatct tgcccatcgc tatttttaat gtctatttta tctcccgagc gctttgaacc aatgcgaata accttagaac cttcatcata ataagccagc catgggcgac cttctgccca ctctcttaac gcttttagtt gtgctacatt gactgcatcg gtgtcttccc agcctgctgc cactcctgct atatgtcggg ttttatggag atcaacgtta ccgatgctaa cctcacccca agacgacacc caccacggat tattatcatt ggaattagaa ccatttattc gaggatccca gcccctggcg tgctctctcg gtctagtccg agaaccagaa cctagagcta cactctgctc agcaccaacc caagtcgaga gatcgggggc aacccctgcg ctgacaccca cagaaacagc tgcgaagctg ctgccgtaac cggaaccagt tcctattgaa accgacagct caggtgccct ccataactta taacaagagt ttaccttaca agccctgtct gcccattgta tattgatcca tgatttacca tactccagat gcccgtagaa ttgtggtcct gcaacttttt ttgtttcacc cagatcaaag attgctgcct cataacctga tgcataggga tgggaaccgc tcctaataga ctgaacacca aaaactccag agagagctct tagcctcttc aaagcgctat cctctctctt tatcgtaagt gaaataccct ctttctctaa actctcctca actgggttga tatctaacac attaaaccca gaaacctgat ctaaaccaca aacataatca tcatcatctt ctgctaaaac aacatttcca tcaaaaacac ttagagaaag aatacatata gtcttacgat cattcatatg gtggtggtgg tggtgagcca tggtat
Expected Protein Sequence
MAHHHHHHND RKTKNDRKTI CILSLSVFDG NVVLAEDDDD YVCGLDQVSG FNVLDINPVE ESLEKEGISL TIKREDSALK RLRALSGVFG VQSIRSGSHP YASGYEAAIF DLGETKKVAG PQFYGHLEYG KSWINIQWAD RACKVNSCYK LWRAPELSVS IGTGSGYGSS FAAVSVGVSA GVAPDLSTWV GAEQSVALGS GSRTRPREHA RGWDPRINGS NSNDNNPWWV SSWGEVSIGN VDLHKTRHIA GVAAGWEDTD AVNVAQLKAL REWAEGRPWL AYYDEGSKVI RIGSKRSGDK IDIKNSDGQD RVLAGVKDGE VSKGSVDAIN GSQLWKTNQT VAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatgatc gtaagactaa gaatgatcgt aagactatat gtattctttc tctaagtgtt tttgatggaa atgttgtttt agcagaagat gatgatgatt atgtttgtgg tttagatcag gtttctgggt ttaatgtgtt agatatcaac ccagttgagg agagtttaga gaaagagggt atttcactta cgataaagag agaggatagc gctttgaaga ggctaagagc tctctctgga gtttttggtg ttcagtctat taggagcggt tcccatccct atgcatcagg ttatgaggca gcaatctttg atctgggtga aacaaaaaaa gttgcaggac cacaattcta cgggcatctg gagtatggta aatcatggat caatatacaa tgggcagaca gggcttgtaa ggtaaactct tgttataagt tatggagggc acctgagctg tcggtttcaa taggaactgg ttccggttac ggcagcagct tcgcagctgt ttctgtgggt gtcagcgcag gggttgcccc cgatctctcg acttgggttg gtgctgagca gagtgtagct ctaggttctg gttctcggac tagaccgaga gagcacgcca ggggctggga tcctcgaata aatggttcta attccaatga taataatccg tggtgggtgt cgtcttgggg tgaggttagc atcggtaacg ttgatctcca taaaacccga catatagcag gagtggcagc aggctgggaa gacaccgatg cagtcaatgt agcacaacta aaagcgttaa gagagtgggc agaaggtcgc ccatggctgg cttattatga tgaaggttct aaggttattc gcattggttc aaagcgctcg ggagataaaa tagacattaa aaatagcgat gggcaagatc gagttttggc tggagtgaag gacggggagg tttctaaggg gtcagttgac gcaataaatg gcagtcaact ttggaaaacc aatcaaacag ttgcgggttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BaquA.17833.a.MB15.GE38118
HARVESTED ON: 6/6/2014
SEQUENCED ON: 6/19/2014
EXPECTED MW: 66kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 32
Validated AA Sequence
MRALSGVFGV QSIRSGSHPY ASGYEAAIFD LGETKKVAGP QFYGHLEYGK SWINIQWADR ACKVNSCYKL WRAPELSVSI GTGSGYGSSF AAVSVGVSAG VAPDLSTWVG AEQSVALGSG SRTRPREHAR GWDPRINGSN SNDNNPWWVS SWGEVSIGNV DLHKTRHIAG VAAGWEDTDA VNVAQLKALR EWAE
Validated NT Sequence
aatnnnanta ccctggagct cagacccagg gtcctggttc gatgagagct ctctctggag tttttggtgt tcagtctatt aggagcggtt cccatcccta tgcatcaggt tatgaggcag caatctttga tctgggtgaa acaaaaaaag ttgcaggacc acaattctac gggcatctgg agtatggtaa atcatggatc aatatacaat gggcagacag ggcttgtaag gtaaactctt gttataagtt atggagggca cctgagctgt cggtttcaat aggaactggt tccggttacg gcagcagctt cgcagctgtt tctgtgggtg tcagcgcagg ggttgccccc gatctctcga cttgggttgg tgctgagcag agtgtagctc taggttctgg ttctcggact agaccgagag agcacgccag gggctgggat cctcgaataa atggttctaa ttccaatgat aataatccgt ggtgggtgtc gtcttggggt gaggttagca tcggtaacgt tgatctccat aaaacccgac atatagcagg agtggcagca ggctgggaag acaccgatgc agtcaatgta gcacaactaa aagcgttaag agagtgggca gaataataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctanngnntt gtgggggggn naanngcttn naaaaacacc nccccccccc caagggtagt gggggggnaa nggggggggt nntccanncn gggggggaaa acggangggg gttccnttng ncggtgngcn gatggtttna aaanaaggcc cccannnaaa aggngggggn gggaggntan tcnngnannn aaagctggcn nntannggcn ggnnncgggn atgtntcncn antcctnnnn ngggagnnct ggatcgtnnn cagcgaccgn ngnntnnnna tncanancnc nnnnacnntn gnnnnggnaa nagngnagga nnngannnnn ncnnnttnnc ntntcgnnct ntnngnnnnn nnncnnnnaa acagnnnnnn ngnngnactt nnncntcnnn nnnnannntn nnagnnnnng annna
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS RALSGVFGVQ SIRSGSHPYA SGYEAAIFDL GETKKVAGPQ FYGHLEYGKS WINIQWADRA CKVNSCYKLW RAPELSVSIG TGSGYGSSFA AVSVGVSAGV APDLSTWVGA EQSVALGSGS RTRPREHARG WDPRINGSNS NDNNPWWVSS WGEVSIGNVD LHKTRHIAGV AAGWEDTDAV NVAQLKALRE WAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgagagc tctctctgga gtttttggtg ttcagtctat taggagcggt tcccatccct atgcatcagg ttatgaggca gcaatctttg atctgggtga aacaaaaaaa gttgcaggac cacaattcta cgggcatctg gagtatggta aatcatggat caatatacaa tgggcagaca gggcttgtaa ggtaaactct tgttataagt tatggagggc acctgagctg tcggtttcaa taggaactgg ttccggttac ggcagcagct tcgcagctgt ttctgtgggt gtcagcgcag gggttgcccc cgatctctcg acttgggttg gtgctgagca gagtgtagct ctaggttctg gttctcggac tagaccgaga gagcacgcca ggggctggga tcctcgaata aatggttcta attccaatga taataatccg tggtgggtgt cgtcttgggg tgaggttagc atcggtaacg ttgatctcca taaaacccga catatagcag gagtggcagc aggctgggaa gacaccgatg cagtcaatgt agcacaacta aaagcgttaa gagagtgggc agaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa