SSGCID: Target Page

BaquA.17330.e

BepA protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BaquA.17330.e
ORGANISM:	Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.17330.e.B1.GE37032	full length		1	218

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPKAKSKETQ TPSSTRTSLP YHYLYSDSNI LKNKYRIKNL DVFLKKCSHD TTKAMVNLCQ ASLPEKLDSS YLRDLHQQLF QNTFEWAETT RDTAFKFEDG TTAVMPEMKR TGWKNPFSID DEIQKGLQKL DRTLAEKNNL QGLSREAFIC ESVEIFISFN HTHPFIEGNE RTQRLFFQQF AQIAGHQLDF LLVTKERMLV ASLAAAQDSQ SRAYETSV

NT Sequence

atgccaaaag caaagtcaaa agaaacgcaa acaccatcct ctacccgcac ctccttacct tatcattatc tttattctga tagcaacatc ctgaaaaata aatatagaat aaaaaatttg gatgtttttc ttaagaaatg ttcgcatgat acgacaaaag caatggtcaa tctgtgccaa gcatccttgc cagaaaagct tgattcttcc tatctaagag atcttcatca acagttattt caaaatacgt ttgaatgggc tgaaactacc cgtgatacag cctttaaatt tgaagatggc accactgctg tcatgccaga aatgaaaaga acagggtgga aaaatccctt ttcaatcgat gatgaaatcc aaaaaggctt acaaaaatta gatcgcacac ttgctgaaaa aaacaatttg caaggcttat cacgtgaagc gtttatctgt gaatcagtgg aaatttttat ctcttttaat catacccacc ctttcataga aggcaatgag cgcacccaac ggttgttttt tcaacaattt gcgcaaatag cgggccatca gctggatttt ttactcgtaa caaaagaacg catgctagtt gccagtcttg cagctgcaca agatagtcaa tctagagcct atgaaacatc tgtttgagga tatctccaat ccagaaaaaa tacctatttt aatttaaaag gagtcatgag ccatatgaaa aatatcaaac gcaatagtcc tacacagccc aaaacattaa gaccgggtga caaattcacc tttacagctc caaggaccag aggccttgaa aacactctca tttcagaaga aacattggct cctttgacaa aaatggaaat agccgaaatg gctgcagaag atgcccgtgt tcacacatgt cgagaccaaa ttcacaaatt gtcagaaatt gtttatggca gctcgaaaac attggaccaa aaaatggtcg agattattaa aaatccaagc gtgggtgccc tgcttgccga acagattgaa aagtctccac actctcttgc aaatcttgca ggttttgacc tcatttgttt taaaagtcgg gcccggataa atgcggaaga aaatgttgaa ctcctcggtt gcgccgtttc aaattttgcc tttgcggtaa gacaggctaa gaaagaaatc acgcaagaac atcaagcaga acaaaaccgc cgtggacaag aggtagcaat gccaagccaa agcttgaaaa atctcctttc catgccaaaa gaattgcagc aaacagcttt agcagcttct cctttgcttc aaaaagagct cgaaagtctt ataaaacaag tcagtaaccg tctctcagga agcgagcaaa gagccctcaa gaacaaggat tatgatgcac tcgtgaaaag tcttggtgtc tcacaacata aagcaaagga aattacacag accgttaatc aagccagaga ggcacagaaa caattgcaaa cacagacagt cagtcgctca aaagtgctcg ccatg

Details for BaquA.17330.e.B1.GE37032

HARVESTED ON:	6/12/2013
SEQUENCED ON:	6/11/2013
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP KAKSKETQTP SSTRTSLPYH YLYSDSNILK NKYRIKNLDV FLKKCSHDTT KAMVNLCQAS LPEKLDSSYL RDLHQQLFQN TFEWAETTRD TAFKFEDGTT AVMPEMKRTG WKNPFSIDDE IQKGLQKLDR TLAEKNNLQG LSREAFICES VEIFISFNHT HPFIEGNERT QRLFFQQFAQ IAGHQLDFLL VTKERMLVAS LAAAQDSQSR AYETSV

Validated NT Sequence

ttgtttaact ttaagaagga ganataccat ggctcaccac caccaccacc atatgccaaa agcaaagtca aaagaaacgc aaacaccatc ctctacccgc acctccttac cttatcatta tctttattct gatagcaaca tcctgaaaaa taaatataga ataaaaaatt tggatgtttt tcttaagaaa tgttcgcatg atacgacaaa agcaatggtc aatctgtgcc aagcatcctt gccagaaaag cttgattctt cctatctaag agatcttcat caacagttat ttcaaaatac gtttgaatgg gctgaaacta cccgtgatac agcctttaaa tttgaagatg gcaccactgc tgtcatgcca gaaatgaaaa gaacagggtg gaaaaatccc ttttcaatcg atgatgaaat ccaaaaaggc ttacaaaaat tagatcgcac acttgctgaa aaaaacaatt tgcaaggctt atcacgtgaa gcgtttatct gtgaatcagt ggaaattttt atctctttta atcataccca ccctttcata gaaggcaatg agcgcaccca acggttgttt tttcaacaat ttgcgcaaat agcgggccat cagctggatt ttttactcgt aacaaaagaa cgcatgctag ttgccagtct tgcagctgca caagatagtc aatctagagc ctatgaaaca tctgtttaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgantt ggctgctgcc accgctganc aataactagc ataacccctt ggggcctcta aannnnnntg gnanggnnnn nnnnnnnnna nnnnanaann nnnnnnnnnn tnnnggttng ccgnnncnnn nnnntntccg nnnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa aagcaaagtc aaaagaaacg caaacaccat cctctacccg cacctcctta ccttatcatt atctttattc tgatagcaac atcctgaaaa ataaatatag aataaaaaat ttggatgttt ttcttaagaa atgttcgcat gatacgacaa aagcaatggt caatctgtgc caagcatcct tgccagaaaa gcttgattct tcctatctaa gagatcttca tcaacagtta tttcaaaata cgtttgaatg ggctgaaact acccgtgata cagcctttaa atttgaagat ggcaccactg ctgtcatgcc agaaatgaaa agaacagggt ggaaaaatcc cttttcaatc gatgatgaaa tccaaaaagg cttacaaaaa ttagatcgca cacttgctga aaaaaacaat ttgcaaggct tatcacgtga agcgtttatc tgtgaatcag tggaaatttt tatctctttt aatcataccc accctttcat agaaggcaat gagcgcaccc aacggttgtt ttttcaacaa tttgcgcaaa tagcgggcca tcagctggat tttttactcg taacaaaaga acgcatgcta gttgccagtc ttgcagctgc acaagatagt caatctagag cctatgaaac atctgtttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at