SSGCID: Target Page

BaquA.17330.d

BepC protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BaquA.17330.d
ORGANISM:	Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.17330.d.B2.GE36068	O91_00901 (double BID)		193	581
BaquA.17330.d.B4.GE37264	BID2		396	525

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.17330.d.B2.PS01712	O91_00901 (double BID)		193	581

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283165.4.peg.1160
RefSeq:	YP_032633.1
UniProt:	A0A0H3M1B7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKKPLPSAF PAVKELRKRY EQSVAETSTP ESLSPRISPK DKRQPVSKLE ELRKRHEQSA VTPEQSLLQA LLYPTPPPQQ PMRTKDREKS TAAVQEGEAL YARAAPQKPP RAKDKEKNTA PIQGGEALYA RAAPQRPPRA KDKEKNTAPI QGGEALYARA APQRPPRAKD KEKNTAPIQG GEALYARAAP QRPPRAKDKE KNTAPIQGGE ALYAMAAPQR PPRAKDKEKN TAPIQGGEAL YAMAAPQKKK TILSKDEISK RLQGSVSIQY CEAEIQHWCR VVYGNPHALK EEIATIKENP MFGEQLSWEV ANHPTSFSKL AGNKMLGIKN SARKEAEAGL PSLCNAIGDY AKTVKREKES ILQNYHAEQK RHEQPMERVQ MVKKPRKPLH LEKEMAPLSN KEIAYKIQRD ISVQYGQAEI QYWCHIVYDN PCLLRERTEE IQKNPAMGEE LSWQVANHPT FFAKFAGHQT LGIKNEARKK AEAGLSSLCN ALDSYTNAVK QAKESIIKEH QEKQNRQGQS AGLAQELQKK QHALSKWPKL PEHSTVTARH ESGETSRQVD SKPPDVRPRK VGTTKAMAFT H

NT Sequence

atgaaaaaaa aaccactccc ttcagcattt cctgcagtga aagaattaag aaaacgctat gaacaatctg tggcagaaac atctactcca gaaagcttat cgccaaggat ttctccaaaa gacaaaagac agcctgtctc gaaactggaa gaattaagaa aacgccatga acaatctgcc gttacaccag aacaaagctt gctacaagca cttctctacc caacacctcc tccacagcaa ccaatgcgca ccaaagatag agagaagagc accgcagctg ttcaagaggg tgaagctctc tacgcaaggg ctgctccaca aaagccacct cgtgcaaaag ataaagagaa aaataccgca cccatccaag ggggtgaagc tctctacgca agggctgctc cacaaaggcc acctcgtgca aaagataaag agaaaaacac cgcacccatc caagggggtg aagctctcta cgcaagggct gctccacaaa ggccacctcg tgcaaaagat aaagagaaaa ataccgcacc catccaaggg ggtgaagctc tctacgcaag ggctgctcca caaaggccac ctcgtgcaaa agataaagag aaaaacaccg cacccatcca agggggtgaa gccctctacg ccatggctgc tccacaaagg ccacctcgtg caaaagataa agagaaaaac accgcaccca tccaaggggg tgaagccctc tacgccatgg ctgctccaca aaagaaaaaa acgattctct cgaaagacga gatttcgaaa aggcttcagg gaagcgtatc gatccaatat tgtgaagcag aaattcaaca ttggtgtaga gttgtttatg gtaacccaca cgctctaaag gaagaaattg caacgattaa agaaaacccc atgtttggag aacagctctc atgggaagtt gcaaatcatc ctacatcttt ctctaagctt gcgggtaaca aaatgcttgg cataaaaaac agcgcacgca aagaagctga agcaggtctt ccttccctct gtaacgccat tggcgattat gcaaagactg taaaaaggga aaaagagagc atcttgcaaa attatcatgc cgagcaaaaa cgccatgaac aaccgatgga gagggtgcaa atggtcaaaa agccgcgaaa gccacttcat cttgaaaagg aaatggctcc tctttcgaac aaagaaattg cttacaagat tcaaagagat atatcggttc aatatggtca agcagaaata caatattggt gccacattgt ttatgacaat ccgtgtctct tgcgggaaag gacagaggag atccaaaaaa atcctgctat gggagaagag ctttcatggc aagttgcaaa tcatcctacg ttttttgcta agttcgcggg tcaccaaacg cttggcataa aaaacgaggc gcgcaaaaaa gctgaagcag gtctttcttc cctttgtaac gctcttgaca gttatacaaa tgctgtaaaa caagcaaaag agagcattat caaggaacat caagaaaaac aaaatcgcca aggacagtct gctgggttag cgcaagaatt gcaaaaaaag cagcacgctt tatcaaagtg gcctaaactg cctgagcatt caacagtcac cgcgcgtcat gagagtggag aaacctccag acaagtagac agcaaaccgc ctgacgttcg accacgcaaa gttgggacaa caaaagcaat ggctttcact cactaa

Details for BaquA.17330.d.B2.GE36068

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	44kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	90

Validated AA Sequence

MAHHHHHHMP PRAKDKEKNT APIQGGEALY AMAAPQKKKT ILSKDEISKR LQGSVSIQYC EAEIQHWCRV VYGNPHALKE EIATIKENPM FGEQLSWEVA NHPTSFSKLA GNKMLGIKNS ARKEAEAGLP SLCNAIGDYA KTVKREKESI LQNYHAEQKR HEQPMERVQM VKKPRKPLHL EKEMAPLSNK EIAYKIQRDT SVQYGQAEIQ YWCHIVYDNP CLLRERTEEI QKNPAMGEEL SWQVANHPTF FAKFAGHQTL GIKNEARKKA EAGLSSLCNA LDSYTNAVKQ AKESIIKEHQ EKQNRQGQSA GLAQELQKKQ HALSKWPKLP EHSTVTARHE SGETSRQVDS KPPDVRPRK

Validated NT Sequence

atggctcacc accaccacca ccatatgcca cctcgtgcaa aagataaaga gaaaaacacc gcacccatcc aagggggtga agccctctac gccatggctg ctccacaaaa gaaaaaaacg attctctcga aagacgagat ttcgaaaagg cttcagggaa gcgtatcgat ccaatattgt gaagcagaaa ttcaacattg gtgtagagtt gtttatggta acccacacgc tctaaaggaa gaaattgcaa cgattaaaga aaaccccatg tttggagaac agctctcatg ggaagttgca aatcatccta catctttctc taagcttgcg ggtaacaaaa tgcttggcat aaaaaacagc gcacgcaaag aagctgaagc aggtcttcct tccctctgta acgccattgg cgattatgca aagactgtaa aaagggaaaa agagagcatc ttgcaaaatt atcatgccga gcaaaaacgc catgaacaac cgatggagag ggtgcaaatg gtcaaaaagc cgcgaaagcc acttcatctt gaaaaggaaa tggctcctct ttcgaacaaa gaaattgctt acaagattca aagagataca tcggttcaat atggtcaagc agaaatacaa tattggtgcc acattgttta tgacaatccg tgtctcttgc gggaaaggac agaggagatc caaaaaaatc ctgctatggg agaagagctt tcatggcaag ttgcaaatca tcctacgttt tttgctaagt tcgcgggtca ccaaacgctt ggcataaaaa acgaggcgcg caaaaaagct gaagcaggtc tttcttccct ttgtaacgct cttgacagtt atacaaatgc tgtaaaacaa gcaaaagaga gcattatcaa ggaacatcaa gaaaaacaaa atcgccaagg acagtctgct gggttagcgc aagaattgca aaaaaagcag cacgctttat caaagtggcc taaactgcct gagcattcaa cagtcaccgc gcgtcatgag agtggagaaa cctccagaca agtagacagc aaaccgcctg acgttcgacc acgcaaag

Expected Protein Sequence

MAHHHHHHMP PRAKDKEKNT APIQGGEALY AMAAPQRPPR AKDKEKNTAP IQGGEALYAM AAPQKKKTIL SKDEISKRLQ GSVSIQYCEA EIQHWCRVVY GNPHALKEEI ATIKENPMFG EQLSWEVANH PTSFSKLAGN KMLGIKNSAR KEAEAGLPSL CNAIGDYAKT VKREKESILQ NYHAEQKRHE QPMERVQMVK KPRKPLHLEK EMAPLSNKEI AYKIQRDISV QYGQAEIQYW CHIVYDNPCL LRERTEEIQK NPAMGEELSW QVANHPTFFA KFAGHQTLGI KNEARKKAEA GLSSLCNALD SYTNAVKQAK ESIIKEHQEK QNRQGQSAGL AQELQKKQHA LSKWPKLPEH STVTARHESG ETSRQVDSKP PDVRPRKVGT TKAMAFTH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccac ctcgtgcaaa agataaagag aaaaacaccg cacccatcca agggggtgaa gccctctacg ccatggctgc tccacaaagg ccacctcgtg caaaagataa agagaaaaac accgcaccca tccaaggggg tgaagccctc tacgccatgg ctgctccaca aaagaaaaaa acgattctct cgaaagacga gatttcgaaa aggcttcagg gaagcgtatc gatccaatat tgtgaagcag aaattcaaca ttggtgtaga gttgtttatg gtaacccaca cgctctaaag gaagaaattg caacgattaa agaaaacccc atgtttggag aacagctctc atgggaagtt gcaaatcatc ctacatcttt ctctaagctt gcgggtaaca aaatgcttgg cataaaaaac agcgcacgca aagaagctga agcaggtctt ccttccctct gtaacgccat tggcgattat gcaaagactg taaaaaggga aaaagagagc atcttgcaaa attatcatgc cgagcaaaaa cgccatgaac aaccgatgga gagggtgcaa atggtcaaaa agccgcgaaa gccacttcat cttgaaaagg aaatggctcc tctttcgaac aaagaaattg cttacaagat tcaaagagat atatcggttc aatatggtca agcagaaata caatattggt gccacattgt ttatgacaat ccgtgtctct tgcgggaaag gacagaggag atccaaaaaa atcctgctat gggagaagag ctttcatggc aagttgcaaa tcatcctacg ttttttgcta agttcgcggg tcaccaaacg cttggcataa aaaacgaggc gcgcaaaaaa gctgaagcag gtctttcttc cctttgtaac gctcttgaca gttatacaaa tgctgtaaaa caagcaaaag agagcattat caaggaacat caagaaaaac aaaatcgcca aggacagtct gctgggttag cgcaagaatt gcaaaaaaag cagcacgctt tatcaaagtg gcctaaactg cctgagcatt caacagtcac cgcgcgtcat gagagtggag aaacctccag acaagtagac agcaaaccgc ctgacgttcg accacgcaaa gttgggacaa caaaagcaat ggctttcact cactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BaquA.17330.d.B4.GE37264

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	16kDa
OBSERVED MW:	16kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA PLSNKEIAYK IQRDTSVQYG QAEIQYWCHI VYDNPCLLRE RTEEIQKNPA MGEELSWQVA NHPTFFAKFA GHQTLGIKNE ARKKAEAGLS SLCNALDSYT NAVKQAKESI IKEHQEKQNR QGQSAGLAQ

Validated NT Sequence

atcttactca cttattgcgc taacccagca gactgtcctt ggcgattttg tttttcttga tgttccttga taatgctctc ttttgcttgt tttacagcat ttgtataact gtcaagagcg ttacaaaggg aagaaagacc tgcttcagct tttttgcgcg cctcgttttt tatgccaagc gtttggtgac ccgcgaactt agcaaaaaac gtaggatgat ttgcaacttg ccatgaaagc tcttctccca tagcaggatt tttttggatc tcctctgtcc tttcccgcaa gagacacgga ttgtcataaa caatgtggca ccaatattgt atttctgctt gaccatattg aaccgatgta tctctttgaa tcttgtaagc aatttctttg ttcgaaagag gagccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHAP LSNKEIAYKI QRDISVQYGQ AEIQYWCHIV YDNPCLLRER TEEIQKNPAM GEELSWQVAN HPTFFAKFAG HQTLGIKNEA RKKAEAGLSS LCNALDSYTN AVKQAKESII KEHQEKQNRQ GQSAGLAQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctcctc tttcgaacaa agaaattgct tacaagattc aaagagatat atcggttcaa tatggtcaag cagaaataca atattggtgc cacattgttt atgacaatcc gtgtctcttg cgggaaagga cagaggagat ccaaaaaaat cctgctatgg gagaagagct ttcatggcaa gttgcaaatc atcctacgtt ttttgctaag ttcgcgggtc accaaacgct tggcataaaa aacgaggcgc gcaaaaaagc tgaagcaggt ctttcttccc tttgtaacgc tcttgacagt tatacaaatg ctgtaaaaca agcaaaagag agcattatca aggaacatca agaaaaacaa aatcgccaag gacagtctgc tgggttagcg caatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BaquA.17330.d.B2.PS01712

PURIFICATION DATe:	12/27/2012
CONCENTRATION:	10.3mg/ml
OBSERVED MW:	44kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	90

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAC CTCGTGCAAA AGATAAAGAG AAAAACACCG CACCCATCCA AGGGGGTGAA GCCCTCTACG CCATGGCTGC TCCACAAAGG CCACCTCGTG CAAAAGATAA AGAGAAAAAC ACCGCACCCA TCCAAGGGGG TGAAGCCCTC TACGCCATGG CTGCTCCACA AAAGAAAAAA ACGATTCTCT CGAAAGACGA GATTTCGAAA AGGCTTCAGG GAAGCGTATC GATCCAATAT TGTGAAGCAG AAATTCAACA TTGGTGTAGA GTTGTTTATG GTAACCCACA CGCTCTAAAG GAAGAAATTG CAACGATTAA AGAAAACCCC ATGTTTGGAG AACAGCTCTC ATGGGAAGTT GCAAATCATC CTACATCTTT CTCTAAGCTT GCGGGTAACA AAATGCTTGG CATAAAAAAC AGCGCACGCA AAGAAGCTGA AGCAGGTCTT CCTTCCCTCT GTAACGCCAT TGGCGATTAT GCAAAGACTG TAAAAAGGGA AAAAGAGAGC ATCTTGCAAA ATTATCATGC CGAGCAAAAA CGCCATGAAC AACCGATGGA GAGGGTGCAA ATGGTCAAAA AGCCGCGAAA GCCACTTCAT CTTGAAAAGG AAATGGCTCC TCTTTCGAAC AAAGAAATTG CTTACAAGAT TCAAAGAGAT ATATCGGTTC AATATGGTCA AGCAGAAATA CAATATTGGT GCCACATTGT TTATGACAAT CCGTGTCTCT TGCGGGAAAG GACAGAGGAG ATCCAAAAAA ATCCTGCTAT GGGAGAAGAG CTTTCATGGC AAGTTGCAAA TCATCCTACG TTTTTTGCTA AGTTCGCGGG TCACCAAACG CTTGGCATAA AAAACGAGGC GCGCAAAAAA GCTGAAGCAG GTCTTTCTTC CCTTTGTAAC GCTCTTGACA GTTATACAAA TGCTGTAAAA CAAGCAAAAG AGAGCATTAT CAAGGAACAT CAAGAAAAAC AAAATCGCCA AGGACAGTCT GCTGGGTTAG CGCAAGAATT GCAAAAAAAG CAGCACGCTT TATCAAAGTG GCCTAAACTG CCTGAGCATT CAACAGTCAC CGCGCGTCAT GAGAGTGGAG AAACCTCCAG ACAAGTAGAC AGCAAACCGC CTGACGTTCG ACCACGCAAA GTTGGGACAA CAAAAGCAAT GGCTTTCACT CACTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT