BaquA.17330.c
BepC protein (VirB-translocated Bartonella effector protein)
| CENTER ID: | BaquA.17330.c |
| ORGANISM: | Bartonella quintana Toulouse |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaquA.17330.c.B2.GE36587 | FIC | 3 | 220 | ||
| BaquA.17330.c.B3.GE37262 | BID | 299 | 435 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4LU4
DEPOSITED: 7/24/2013
DETERMINATION: XRay
CLONE: BaquA.17330.c.B2.GE36587
PROTEIN: BaquA.17330.c.B2.PS01833
4N67
DEPOSITED: 10/11/2013
DETERMINATION: XRay
CLONE: BaquA.17330.c.B2.GE36587
PROTEIN: BaquA.17330.c.B2.PS01833
Publications by SSGCID
Evolutionary Diversification of Host-Targeted Bartonella Effectors Proteins Derived from a Conserved FicTA Toxin-Antitoxin Module
de Beer T, Dehio C, Edwards T, Harms A, Myler P, Phan I, Schirmer T, Dranow D, Tamegger S, Dietz N
Microorganisms - 2021
volume 9, issue 8, pages 1645
PMID: 34442725; PMCID: PMC8401265
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_171090 |
| PATRIC ID: | fig|283165.4.peg.1158 |
| RefSeq: | YP_032631.1 |
| UniProt: | A0A0H3LV04 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLEHNYFYKN SATLKNKHGI KNPRKLYERC AHETAREAVN FRLEPPPGKF DAAYLRTIHW CLFHNTFEWA GVTRDQPFTF EDGSTACMPA MRPKGYKVPF AVGSQIQREL KKLEQRLTAK NNLQGLSRQE FAANAAEVFT ALDHAHPFRK GNGRTQRMFM EKLGQAAGYK IDFSLITKER MTYASIEAMQ HNNPEPMKDL FEDITHPQKS LLLKEFISQM RSARLDEINN HIVLAAKEGV TYDGIYKGSS AEGFVIEVEG GTFIVGHKDD LKPEQVKILQ NGDFISFQKN NVQNMRETLI PSEILAPLTN EILAERLVNH CGVESYRHEV ECLSKIVYGN TQALSQMIET INIDPSLGEQ FVDHIIQNPK SVGKLAGKKI LGLRSPARKR AEETVSQLSD TLKSYADIAH QTMADIIEQH SKEQRRTARS VENPGKDLQN LFALFPEQQR EALSHSPTLQ QQLHRFSRQL QNRLSSEERR AIQENDCTRL SCLLGVSASK AKDIAQIVKH TKEAQCQMRT LKVCRSASMA LTS
NT Sequence
atgttagagc ataattattt ttataaaaac agcgcaacac tgaagaataa acatggcata aaaaacccgc gaaaactgta tgaacgctgt gctcatgaga cagccagaga ggctgtaaat tttcgccttg aaccgccacc agggaaattt gatgccgctt atctaaggac aattcactgg tgccttttcc ataacacttt tgaatgggcc ggtgttaccc gagatcagcc ctttacattt gaagatggca gcactgcatg tatgccagct atgcgaccaa aaggttataa ggttcctttt gctgtcggtt cacaaattca aagagagctt aaaaaattag aacaaagact aaccgcgaag aataatttac aaggcttatc gcgccaagaa tttgctgcaa atgctgctga agtttttaca gctctcgacc acgcgcatcc tttcagaaaa ggcaatgggc gcacacaacg aatgtttatg gaaaaactcg gacaagcggc aggctataag attgattttt ctttgatcac aaaagaacgc atgacatatg ccagcattga agcaatgcaa cataacaatc cagaacccat gaaagatctt tttgaggata tcactcaccc tcaaaaatcc cttcttttaa aggaatttat ctctcagatg agaagcgcta gacttgatga aattaacaat catattgttt tggcagcaaa agaaggtgtg acctatgatg gcatttataa aggttcttca gctgaaggtt ttgttataga agtagaaggt ggcactttca tcgtcgggca caaagatgat cttaagccag agcaagtgaa aatattacag aatggtgatt tcatctcctt tcagaaaaac aatgttcaaa acatgagaga aacacttatc ccaagcgaga tattagcgcc tctcaccaat gagatccttg ccgaaaggct cgtaaaccat tgtggggttg aatcataccg ccatgaagtc gagtgtttat caaaaattgt ttatggcaac acacaagcat taagccaaat gattgagaca atcaacatag atccaagttt aggcgaacag tttgtcgatc acattatcca aaatcctaaa tcagttggta agcttgccgg gaaaaaaatc ctgggtctaa gaagcccagc gcgcaaacgt gcggaagaaa ctgtttcaca gctgagtgac acacttaaaa gttatgccga tatagcacat cagaccatgg ctgacatcat agagcaacac tcaaaagaac aaagacgcac agcgcgctct gtcgaaaatc ctgggaaaga cttgcaaaac ctttttgcct tgttcccaga acaacaaaga gaagccttgt ctcattcccc tacgctgcaa caacaactcc atcgtttttc gcgtcaattg caaaatcgtt tatcatcaga ggagcgtcga gccatacaag aaaacgattg cacaagactt tcttgtctgc ttggtgtatc ggcaagcaaa gcaaaagaca ttgctcaaat tgtgaagcat accaaagagg cacaatgtca gatgcgtacc cttaaagtct gccgctccgc atcgatggct ctgaccagct aa
Details for BaquA.17330.c.B2.GE36587
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 8/22/2013 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHME HNYFYKNSAT LKNKHGIKNP RKLYERCAHE TAREAVNFRL EPPPGKFDAA YLRTIHWCLF HKTFEWAGVT RDQPFTFEDG STACMPAMRP KGYKVPFAVG SQIQRELKKL EQRLTAKNNL QGLSRQEFAA NAAEVFTALD HAHPFRKGNG RTQRMFMEKL GQAAGYKIDF SLITKERMTY ASIEAMQHNN PEPMKDLFED ITHPQKSLLL KEFISQM
Validated NT Sequence
atggctcacc accaccacca ccatatggag cataattatt tttataaaaa cagcgcaaca ctgaagaata aacatggcat aaaaaacccg cgaaaactgt atgaacgctg tgctcatgag acagccagag aggctgtaaa ttttcgcctt gaaccgccac cagggaaatt tgatgccgct tatctaagga caattcactg gtgccttttc cataaaactt ttgaatgggc cggtgttacc cgagatcagc cctttacatt tgaagatggc agcactgcat gtatgccagc tatgcgacca aaaggttata aggttccttt tgctgtcggt tcacaaattc aaagagagct taaaaaatta gaacaaagac taaccgcgaa gaataattta caaggcttat cgcgccaaga atttgctgca aatgctgctg aagtttttac agctctcgac cacgcgcatc ctttcagaaa aggcaatggg cgcacacaac gaatgtttat ggaaaaactc ggacaagcgg caggctataa gattgatttt tctttgatca caaaagaacg catgacatat gccagcattg aagcaatgca acataacaat ccagaaccca tgaaagatct ttttgaggat atcactcacc ctcaaaaatc ccttctttta aaggaattta tctctcagat gtaagtgagt aagat
Expected Protein Sequence
MAHHHHHHME HNYFYKNSAT LKNKHGIKNP RKLYERCAHE TAREAVNFRL EPPPGKFDAA YLRTIHWCLF HNTFEWAGVT RDQPFTFEDG STACMPAMRP KGYKVPFAVG SQIQRELKKL EQRLTAKNNL QGLSRQEFAA NAAEVFTALD HAHPFRKGNG RTQRMFMEKL GQAAGYKIDF SLITKERMTY ASIEAMQHNN PEPMKDLFED ITHPQKSLLL KEFISQM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggagc ataattattt ttataaaaac agcgcaacac tgaagaataa acatggcata aaaaacccgc gaaaactgta tgaacgctgt gctcatgaga cagccagaga ggctgtaaat tttcgccttg aaccgccacc agggaaattt gatgccgctt atctaaggac aattcactgg tgccttttcc ataacacttt tgaatgggcc ggtgttaccc gagatcagcc ctttacattt gaagatggca gcactgcatg tatgccagct atgcgaccaa aaggttataa ggttcctttt gctgtcggtt cacaaattca aagagagctt aaaaaattag aacaaagact aaccgcgaag aataatttac aaggcttatc gcgccaagaa tttgctgcaa atgctgctga agtttttaca gctctcgacc acgcgcatcc tttcagaaaa ggcaatgggc gcacacaacg aatgtttatg gaaaaactcg gacaagcggc aggctataag attgattttt ctttgatcac aaaagaacgc atgacatatg ccagcattga agcaatgcaa cataacaatc cagaacccat gaaagatctt tttgaggata tcactcaccc tcaaaaatcc cttcttttaa aggaatttat ctctcagatg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BaquA.17330.c.B3.GE37262
| HARVESTED ON: | 8/28/2013 |
| SEQUENCED ON: | 9/3/2013 |
| EXPECTED MW: | 16kDa |
| OBSERVED MW: | 15kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML IPSEILAPLT NEILAERLVN HCGVESYRHE VECLSKIVYG NTQALSQMIE TINIDPSLGE QFVDHIIQNP KSVGKLAGKK ILGLRSPARK RAEETVSQLS DTLKSYADIA HQTMADIIEQ HSKEQRRTAR SVENPG
Validated NT Sequence
atcttactca cttacccagg attttcgaca gagcgcgctg tgcgtctttg ttcttttgag tgttgctcta tgatgtcagc catggtctga tgtgctatat cggcataact tttaagtgtg tcactaagct gtgaaacagt ttcttccgca cgtttgcgcg ctgggcttct tagacccagg atttttttcc cggcaagctt accaactgat ttaggatttt ggataatgtg atcgacaaac tgttcgccta aacttggatc tatgttgatt gtctcaatca tttggcttaa tgcttgtgtg ttgccataaa caatttttga taaacactcg acttcatggc ggtatgattc aaccccacaa tggtttacga gcctttcggc aaggatctca ttggtgagag gcgctaatat ctcgcttggg ataagcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHLI PSEILAPLTN EILAERLVNH CGVESYRHEV ECLSKIVYGN TQALSQMIET INIDPSLGEQ FVDHIIQNPK SVGKLAGKKI LGLRSPARKR AEETVSQLSD TLKSYADIAH QTMADIIEQH SKEQRRTARS VENPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcttatcc caagcgagat attagcgcct ctcaccaatg agatccttgc cgaaaggctc gtaaaccatt gtggggttga atcataccgc catgaagtcg agtgtttatc aaaaattgtt tatggcaaca cacaagcatt aagccaaatg attgagacaa tcaacataga tccaagttta ggcgaacagt ttgtcgatca cattatccaa aatcctaaat cagttggtaa gcttgccggg aaaaaaatcc tgggtctaag aagcccagcg cgcaaacgtg cggaagaaac tgtttcacag ctgagtgaca cacttaaaag ttatgccgat atagcacatc agaccatggc tgacatcata gagcaacact caaaagaaca aagacgcaca gcgcgctctg tcgaaaatcc tgggtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat