BaquA.17330.a

BepE protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaquA.17330.a
ORGANISM: Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.17330.a.B2.PS01962 BID 167 303

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283165.4.peg.1159
RefSeq: YP_032632.1
UniProt: A0A0H3LUU1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKNQPSSPS IPRSVEEARR RYEQAPPGSA SPPEPLYATV NKKTSHTAKK PEQGAPQAPL FAASSPQRPP RLRDRELHKT TIKEEEIVYA SLVHPPHRDH KHPRKNPDNE TLYATVAPQS QAMSPTREKE EGIVYASLVH PPHTDHRHPR KNPDNETLYA TVASQNLARQ SQAMSLTREE MATRIQQSPS VQAYQQEVSH WCQIVFGNSR VLQERVQEIL QKPNLAEEVS WQVAENPQAI HKLAGISLCG ITNSARRHAE AGLSHLCNAI ENYGDAVKQA HENIMHVPHA EARRHGEDRT RSAQSLQQPH HPERGSASLS NEEMLGMVQS HTSIQRYHAQ IDYWCKIVFG NSRVLQERVQ EILQKPNLAE EVSWQVAENP QAIHKLAGIS LCGITNSARR HAEAGLSHLC NAIENYGDAV KQVKESIVQT HQTRQNRQEQ ASGLSQSVQK QQTLSKPPKL PERPAVTARN EETSRQQAHQ KAPDVRPRRI RETKAIAFAS
NT Sequence
atgaaaaaaa accaaccatc ctctccttct atcccccgtt ctgtggaaga agcaagaaga cgttatgaac aagctcctcc cggaagcgca tctccaccag aacccctgta cgcaacagtg aacaaaaaaa catctcatac agcgaaaaag ccagaacaag gagcgccaca agcgcctctc ttcgcagcga gttctccaca aaggccgcct cgcctgagag acagggagct ccataaaaca acaatcaaag aggaggaaat cgtatatgca tcgcttgttc atccgcctca cagggaccac aaacacccga gaaagaatcc agacaacgaa accctctacg caacggttgc tccccaaagt caagcaatgt ctcccacgag agagaaagag gaggggatag tatatgcatc gcttgttcat ccacctcaca cggaccacag acacccgaga aagaatccag acaatgaaac cctttacgca acggttgctt ctcaaaatct agctcgccaa agtcaagcaa tgtctctcac gagagaggaa atggcgacca ggattcaaca gagcccatcg gtccaagcat atcaacaaga agtctcacat tggtgccaaa ttgtttttgg caattcgcgt gttttgcaag aaagagtgca ggagattctc caaaaaccca atttggcaga agaggtctca tggcaagttg cagaaaatcc ccaggctatt cataagcttg cagggattag tctgtgtggc ataacaaata gtgcccgcag acatgctgaa gcaggccttt cacatctttg taatgccatt gaaaactatg gagatgctgt aaaacaagca cacgaaaaca ttatgcacgt tcctcacgcg gaagcaagac gtcatggaga ggacaggaca cgcagtgccc aaagtctgca acagccgcat catcctgaaa ggggaagtgc atctctctca aatgaggaaa tgctggggat ggttcaaagc catacatcca tccaaagata tcatgcacaa attgactatt ggtgcaaaat tgtttttggc aattcgcgtg ttttgcaaga aagagtacag gagattctcc aaaaacccaa tttggcagaa gaggtctcat ggcaagttgc agaaaatccc caggctattc ataagcttgc agggattagt ctgtgtggca taacaaatag tgcccgcaga catgctgaag caggcctttc acatctttgt aatgccattg aaaactatgg agatgctgta aaacaagtaa aggaaagtat tgtgcagacg caccagacga ggcaaaatcg ccaagaacag gcttctggat tgtctcagag cgtgcaaaaa caacaaacct tgtctaagcc tcctaaactg cctgaacgtc cagctgtaac cgcacgtaat gaggaaactt ccagacagca agcacaccag aaagcaccag atgttcgacc tcgcagaatt cgggaaacga aagcaatagc tttcgccagc taa
Details for BaquA.17330.a.B2.PS01962
PURIFICATION DATe: 1/7/2014
CONCENTRATION: 12.8mg/ml
OBSERVED MW: 16kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 101
Protocol Notes
notes unavailable
Validated AA Sequence
MLARQSQAMS LTREEMATRI QQSPSVQAYQ QEVSHWCQIV FGNSRVLQER VQEILQKPNL AEEVSWQVAE NPQAIHKLAG ISMCGITNSA RRHAEAGLSH LCTAIENYGD AVKQAHENIM HVPHAETRRH GEDRTRSAQV SKIGSGC
Validated NT Sequence
tctttcgggg gcttgttagc agccggatcc tatcttactc acttgggcac tgcgtgtcct gtcctctcca tgacgtcttg tttccgcgtg aggaacgtgc ataatgtttt cgtgtgcttg ttttacagca tctccatagt tttcaatggc agtacaaaga tgtgaaaggc ctgcttcagc atgtctgcgg gcactatttg ttatgccaca catactaatc cctgcaagct tatgaatagc ctggggattt tctgcaactt gccatgagac ctcttctgcc aaattgggtt tttggagaat ctcctgcact ctttcttgca aaacacgcga attgccaaaa acaatttggc accaatgtga gacttcttgt tgatatgctt ggaccgatgg gctctgttga atcctggtcg ccatttcctc tctcgtgaga gacattgctt gactttggcg agctagcata tggtggtggt ggtggtgagc ca
Expressed Protein Sequence
MAHHHHHHLA RQSQAMSLTR EEMATRIQQS PSVQAYQQEV SHWCQIVFGN SRVLQERVQE ILQKPNLAEE VSWQVAENPQ AIHKLAGISL CGITNSARRH AEAGLSHLCN AIENYGDAVK QAHENIMHVP HAEARRHGED RTRSA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTAGCTC GCCAAAGTCA AGCAATGTCT CTCACGAGAG AGGAAATGGC GACCAGGATT CAACAGAGCC CATCGGTCCA AGCATATCAA CAAGAAGTCT CACATTGGTG CCAAATTGTT TTTGGCAATT CGCGTGTTTT GCAAGAAAGA GTGCAGGAGA TTCTCCAAAA ACCCAATTTG GCAGAAGAGG TCTCATGGCA AGTTGCAGAA AATCCCCAGG CTATTCATAA GCTTGCAGGG ATTAGTCTGT GTGGCATAAC AAATAGTGCC CGCAGACATG CTGAAGCAGG CCTTTCACAT CTTTGTAATG CCATTGAAAA CTATGGAGAT GCTGTAAAAC AAGCACACGA AAACATTATG CACGTTCCTC ACGCGGAAGC AAGACGTCAT GGAGAGGACA GGACACGCAG TGCCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT