BaquA.17156.a
Sensory transduction regulatory protein PhyR
| CENTER ID: | BaquA.17156.a |
| ORGANISM: | Bartonella quintana Toulouse |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaquA.17156.a.DG13.GE00404 | full length | 1 | 265 | ||
| BaquA.17156.a.DG14.GE00405 | full length | 1 | 265 | ||
| BaquA.17156.a.DG15.GE00406 | full length | 1 | 265 | ||
| BaquA.17156.a.DG16.GE00407 | full length | 1 | 265 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaquA.17156.a.DG12.PD00336 | full length | 1 | 265 | ||
| BaquA.17156.a.DG11.PD00341 | full length | 1 | 265 | ||
| BaquA.17156.a.DG13.PD00386 | full length | 1 | 265 | ||
| BaquA.17156.a.DG14.PD00387 | full length | 1 | 265 |
Structures
4QIC
DEPOSITED: 5/30/2014
DETERMINATION: XRay
CLONE: BaquA.17156.a.CB1.GE05919
PROTEIN: BaquA.17156.a.CB1.PS01826
5UXW
DEPOSITED: 2/23/2017
DETERMINATION: XRay
CLONE: BaquA.17156.a.CB1.GE05919
PROTEIN: BaquA.17156.a.CB1.PS02210
5UXV
DEPOSITED: 2/23/2017
DETERMINATION: XRay
CLONE: BaquA.17156.a.DG11.GE00307
PROTEIN: BaquA.17156.a.DG11.PD00341
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|283165.4.peg.1191 |
| RefSeq: | YP_032651.1 |
| UniProt: | A0A0H3LUV4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLSTRIAPH LPYLRRFARS VTGSQSSGDA YVSAMLEALI ADISIFPRAS SDRIGTYWLF CHLFDQTTPN IPEPLPQFGL EQKTSAKLSY LTPRARQAFL LIAVEGFNEQ EASEIMNLDA RDFRKLLNQA SIDISQQIAT QVMIIEDEPL IAMDIEQMVE SLGHQVVGIA RTRKEAVVMY HQKKPRLILA DIQLADNSSG IDAVNDILQN DRIPVIFITA FPERLLTGER PEPTFLVTKP FNPDMVKALI SQALFFKENA SKAA
NT Sequence
atgtcattat caacgcgcat cgcaccgcat ctcccctatc ttcgacgttt tgcccgatct gtcacaggta gccaatcatc cggtgacgct tatgtgtcag caatgttgga agcactgatt gctgatattt ctatttttcc aagggcatcc agtgaccgca tcggtaccta ttggcttttt tgccaccttt ttgaccaaac gacgcctaat atacccgagc cactgccaca gtttggtctt gaacaaaaga ccagtgcaaa attatcgtat cttacaccac gtgcacgcca agcatttttg ttgattgccg ttgaaggatt taatgaacaa gaagccagcg aaatcatgaa tcttgatgca agggatttcc gtaagcttct caaccaagca tcgattgata tttctcaaca aattgccaca caggttatga ttattgagga tgagcctctt attgcgatgg atattgagca aatggtagaa agcttaggac atcaagttgt agggatagca cgaacccgca aagaagctgt cgtaatgtat catcaaaaaa agccacggtt gattttagct gatattcaat tggctgataa cagttcgggt attgatgcgg ttaatgacat tttgcaaaat gaccgtatac cggtgatttt tattaccgcg tttcctgaaa gattactaac cggagagcgt ccagaaccaa cttttttagt taccaaaccc tttaatcccg atatggtgaa agcacttatt tcacaggctt tattttttaa agagaatgcg tccaaagctg cttag
Details for BaquA.17156.a.DG13.GE00404
| HARVESTED ON: | 1/19/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSL STRIAPHLPY LRRFARSVTG SQSSGDAYVS AMLEALVADI SIFPRASCDR IGTYWLFCHL FDQTTPNIPE PLPQFGLEQK TSAKLSYLTP RARQAFLLIA VEGFNEQEAS EIMNLDARDF RKLLNQASID ISQQIATQVM IIEDEPLIAM DIEQMVESLG HQVVGIARTR KEAVVMYHQK KPRLILAAIQ LADNSSGIDA VNDILQNDRI PVIFITAFPE RLLTGERPEP TFLVTKPFNP DMVKALISQA LFFKENASKA A
Full NT Sequence (Expression Vector + Insert)
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggagaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggatcc atgtctctga gcactcgtat cgctccgcac ctgccgtact tgcgtcgttt cgctagatct gtgaccggtt ctcagagctc cggtgatgcg tacgtctctg ccatgcttga ggctttagtc gcagacatct ctatctttcc gcgtgcatcg tgtgaccgca taggcactta ctggctgttt tgccacttgt tcgatcagac tactccgaac attccggaac cgctcccgca gtttggtctg gaacagaaaa ctagcgcaaa actgagctac ctgaccccgc gcgcgcgtca ggcattcctg ctgatcgctg ttgaaggttt caacgaacag gaagcatctg aaatcatgaa cctggacgcg cgtgatttcc gtaaactgct gaaccaggcg tctattgaca tctcccagca gatcgctact caggttatga ttatcgaaga cgaaccgtta atcgcaatgg acattgaaca gatggttgaa tctctgggtc accaggttgt aggtatcgct cgtactcgta aagaagctgt agttatgtac caccagaaga agccgcgcct tattcttgct gctatccagt tagctgacaa ctcctccggt atcgacgctg tcaacgatat cctgcagaac gaccgtatcc cggtaatctt tatcaccgct tttccggaac gtctgctgac cggtgaacgc ccagaaccga ccttcctggt cacaaagccg tttaacccgg atatggttaa agctctgatc tcccaggcac tgttcttcaa agaaaacgct tccaaagctg cttgataagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc aaggagatgg cgcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc ttatc
Details for BaquA.17156.a.DG14.GE00405
| HARVESTED ON: | 1/19/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSL STRIAPHLPY LRRFARSVTG SQSSGDAYVS AMLEALVADI SIFPRASCDR IGTYWLFCHL FDQTTPNIPE PLPQFGLEQK TSAKLSYLTP RARQAFLLIA VEGFNEQEAS EIMNLDARDF RKLLNQASID ISQQIATQVM IIEDEPLIAM DIEQMVESLG HQVVGIARTR KEAVVMYHQK KPRLILADIQ LAANSSGIDA VNDILQNDRI PVIFITAFPE RLLTGERPEP TFLVTKPFNP DMVKALISQA LFFKENASKA A
Full NT Sequence (Expression Vector + Insert)
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggagaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggatcc atgtctctga gcactcgtat cgctccgcac ctgccgtact tgcgtcgttt cgctagatct gtgaccggtt ctcagagctc cggtgatgcg tacgtctctg ccatgcttga ggctttagtc gcagacatct ctatctttcc gcgtgcatcg tgtgaccgca taggcactta ctggctgttt tgccacttgt tcgatcagac tactccgaac attccggaac cgctcccgca gtttggtctg gaacagaaaa ctagcgcaaa actgagctac ctgaccccgc gcgcgcgtca ggcattcctg ctgatcgctg ttgaaggttt caacgaacag gaagcatctg aaatcatgaa cctggacgcg cgtgatttcc gtaaactgct gaaccaggcg tctattgaca tctcccagca gatcgctact caggttatga ttatcgaaga cgaaccgtta atcgcaatgg acattgaaca gatggttgaa tctctgggtc accaggttgt aggtatcgct cgtactcgta aagaagctgt agttatgtac caccagaaga agccgcgcct tattcttgct gacatccagt tagctgctaa ctcctccggt atcgacgctg tcaacgatat cctgcagaac gaccgtatcc cggtaatctt tatcaccgct tttccggaac gtctgctgac cggtgaacgc ccagaaccga ccttcctggt cacaaagccg tttaacccgg atatggttaa agctctgatc tcccaggcac tgttcttcaa agaaaacgct tccaaagctg cttgataagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc aaggagatgg cgcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc ttatc
Details for BaquA.17156.a.DG15.GE00406
| HARVESTED ON: | 1/19/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSL STRIAPHLPY LRRFARSVTG SQSSGDAYVS AMLEALIADI SIFPRASSDR IGTYWLFCHL FDQTTPNIPE PLPQFGLEQK TSAKLSYLTP RARQAFLLIA VEGFNEQEAS EIMNLDARDF RKLLNQASID ISQQIATQVM IIEDEPLIAM DIEQMVESLG HQVVGIARTR KEAVVMYHQK KPRLILAAIQ LADNSSGIDA VNDILQNDRI PVIFITAFPE RLLTGERPEP TFLVTKPFNP DMVKALISQA LFFKENASKA A
Full NT Sequence (Expression Vector + Insert)
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggagaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggatcc atgtctctga gcactcgtat cgctccgcac ctgccgtact tgcgtcgttt cgctagatct gtgaccggtt ctcagagctc cggtgatgcg tacgtctctg ccatgcttga ggctttaatc gcagacatct ctatctttcc gcgtgcatcg tctgaccgca taggcactta ctggctgttt tgccacttgt tcgatcagac tactccgaac attccggaac cgctcccgca gtttggtctg gaacagaaaa ctagcgcaaa actgagctac ctgaccccgc gcgcgcgtca ggcattcctg ctgatcgctg ttgaaggttt caacgaacag gaagcatctg aaatcatgaa cctggacgcg cgtgatttcc gtaaactgct gaaccaggcg tctattgaca tctcccagca gatcgctact caggttatga ttatcgaaga cgaaccgtta atcgcaatgg acattgaaca gatggttgaa tctctgggtc accaggttgt aggtatcgct cgtactcgta aagaagctgt agttatgtac caccagaaga agccgcgcct tattcttgct gctatccagt tagctgacaa ctcctccggt atcgacgctg tcaacgatat cctgcagaac gaccgtatcc cggtaatctt tatcaccgct tttccggaac gtctgctgac cggtgaacgc ccagaaccga ccttcctggt cacaaagccg tttaacccgg atatggttaa agctctgatc tcccaggcac tgttcttcaa agaaaacgct tccaaagctg cttgataagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc aaggagatgg cgcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc ttatc
Details for BaquA.17156.a.DG16.GE00407
| HARVESTED ON: | 1/19/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSL STRIAPHLPY LRRFARSVTG SQSSGDAYVS AMLEALIADI SIFPRASSDR IGTYWLFCHL FDQTTPNIPE PLPQFGLEQK TSAKLSYLTP RARQAFLLIA VEGFNEQEAS EIMNLDARDF RKLLNQASID ISQQIATQVM IIEDEPLIAM DIEQMVESLG HQVVGIARTR KEAVVMYHQK KPRLILADIQ LAANSSGIDA VNDILQNDRI PVIFITAFPE RLLTGERPEP TFLVTKPFNP DMVKALISQA LFFKENASKA A
Full NT Sequence (Expression Vector + Insert)
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggagaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggatcc atgtctctga gcactcgtat cgctccgcac ctgccgtact tgcgtcgttt cgctagatct gtgaccggtt ctcagagctc cggtgatgcg tacgtctctg ccatgcttga ggctttaatc gcagacatct ctatctttcc gcgtgcatcg tctgaccgca taggcactta ctggctgttt tgccacttgt tcgatcagac tactccgaac attccggaac cgctcccgca gtttggtctg gaacagaaaa ctagcgcaaa actgagctac ctgaccccgc gcgcgcgtca ggcattcctg ctgatcgctg ttgaaggttt caacgaacag gaagcatctg aaatcatgaa cctggacgcg cgtgatttcc gtaaactgct gaaccaggcg tctattgaca tctcccagca gatcgctact caggttatga ttatcgaaga cgaaccgtta atcgcaatgg acattgaaca gatggttgaa tctctgggtc accaggttgt aggtatcgct cgtactcgta aagaagctgt agttatgtac caccagaaga agccgcgcct tattcttgct gacatccagt tagctgctaa ctcctccggt atcgacgctg tcaacgatat cctgcagaac gaccgtatcc cggtaatctt tatcaccgct tttccggaac gtctgctgac cggtgaacgc ccagaaccga ccttcctggt cacaaagccg tttaacccgg atatggttaa agctctgatc tcccaggcac tgttcttcaa agaaaacgct tccaaagctg cttgataagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc aaggagatgg cgcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc ttatc
Details for BaquA.17156.a.DG12.PD00336
| PURIFICATION DATe: | 9/2/2014 |
| CONCENTRATION: | 20.01mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSL STRIAPHLPY LRRFARSVTG SQSSGDAYVS AMLEALIADI SIFPRASSDR IGTYWLFCHL FDQTTPNIPE PLPQFGLEQK TSAKLSYLTP RARQAFLLIA VEGFNEQEAS EIMNLDARDF RKLLNQASID ISQQIATQVM IIEDEPLIAM DIEQMVESLG HQVVGIARTR KEAVVMYHQK KPRLILADIQ LADNSSGIDA VNDILQNDRI PVIFITAFPE RLLTGERPEP TFLVTKPFNP DMVKALISQA LFFKENASKA A
Full NT Sequence (Expression Vector + Insert)
ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC ATGTCTCTGA GCACTCGTAT CGCTCCGCAC CTGCCGTACT TGCGTCGTTT CGCTAGATCT GTGACCGGTT CTCAGAGCTC CGGTGATGCG TACGTCTCTG CCATGCTTGA GGCTTTAATC GCAGACATCT CTATCTTTCC GCGTGCATCG TCTGACCGCA TAGGCACTTA CTGGCTGTTT TGCCACTTGT TCGATCAGAC TACTCCGAAC ATTCCGGAAC CGCTCCCGCA GTTTGGTCTG GAACAGAAAA CTAGCGCAAA ACTGAGCTAC CTGACCCCGC GCGCGCGTCA GGCATTCCTG CTGATCGCTG TTGAAGGTTT CAACGAACAG GAAGCATCTG AAATCATGAA CCTGGACGCG CGTGATTTCC GTAAACTGCT GAACCAGGCG TCTATTGACA TCTCCCAGCA GATCGCTACT CAGGTTATGA TTATCGAAGA CGAACCGTTA ATCGCAATGG ACATTGAACA GATGGTTGAA TCTCTGGGTC ACCAGGTTGT AGGTATCGCT CGTACTCGTA AAGAAGCTGT AGTTATGTAC CACCAGAAGA AGCCGCGCCT TATTCTTGCT GACATCCAGT TAGCTGACAA CTCCTCCGGT ATCGACGCTG TCAACGATAT CCTGCAGAAC GACCGTATCC CGGTAATCTT TATCACCGCT TTTCCGGAAC GTCTGCTGAC CGGTGAACGC CCAGAACCGA CCTTCCTGGT CACAAAGCCG TTTAACCCGG ATATGGTTAA AGCTCTGATC TCCCAGGCAC TGTTCTTCAA AGAAAACGCT TCCAAAGCTG CTTGATAAGC TTGGCTGTTT TGGCGGATGA GAGAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG AATTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG AAACGCCGTA GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAATTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTTG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTT GAACAACACT CAACCCTATC TCGGGCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT TACAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATAGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC AAGGAGATGG CGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCTGCTCAT GTTTGACAGC TTATC
Details for BaquA.17156.a.DG11.PD00341
| PURIFICATION DATe: | 9/17/2014 |
| CONCENTRATION: | 19.88mg/ml |
| OBSERVED MW: | 41kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 70 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSL STRIAPHLPY LRRFARSVTG SQSSGDAYVS AMLEALVADI SIFPRASCDR IGTYWLFCHL FDQTTPNIPE PLPQFGLEQK TSAKLSYLTP RARQAFLLIA VEGFNEQEAS EIMNLDARDF RKLLNQASID ISQQIATQVM IIEDEPLIAM DIEQMVESLG HQVVGIARTR KEAVVMYHQK KPRLILADIQ LADNSSGIDA VNDILQNDRI PVIFITAFPE RLLTGERPEP TFLVTKPFNP DMVKALISQA LFFKENASKA A
Full NT Sequence (Expression Vector + Insert)
ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC ATGTCTCTGA GCACTCGTAT CGCTCCGCAC CTGCCGTACT TGCGTCGTTT CGCTAGATCT GTGACCGGTT CTCAGAGCTC CGGTGATGCG TACGTCTCTG CCATGCTTGA GGCTTTAGTC GCAGACATCT CTATCTTTCC GCGTGCATCG TGTGACCGCA TAGGCACTTA CTGGCTGTTT TGCCACTTGT TCGATCAGAC TACTCCGAAC ATTCCGGAAC CGCTCCCGCA GTTTGGTCTG GAACAGAAAA CTAGCGCAAA ACTGAGCTAC CTGACCCCGC GCGCGCGTCA GGCATTCCTG CTGATCGCTG TTGAAGGTTT CAACGAACAG GAAGCATCTG AAATCATGAA CCTGGACGCG CGTGATTTCC GTAAACTGCT GAACCAGGCG TCTATTGACA TCTCCCAGCA GATCGCTACT CAGGTTATGA TTATCGAAGA CGAACCGTTA ATCGCAATGG ACATTGAACA GATGGTTGAA TCTCTGGGTC ACCAGGTTGT AGGTATCGCT CGTACTCGTA AAGAAGCTGT AGTTATGTAC CACCAGAAGA AGCCGCGCCT TATTCTTGCT GACATCCAGT TAGCTGACAA CTCCTCCGGT ATCGACGCTG TCAACGATAT CCTGCAGAAC GACCGTATCC CGGTAATCTT TATCACCGCT TTTCCGGAAC GTCTGCTGAC CGGTGAACGC CCAGAACCGA CCTTCCTGGT CACAAAGCCG TTTAACCCGG ATATGGTTAA AGCTCTGATC TCCCAGGCAC TGTTCTTCAA AGAAAACGCT TCCAAAGCTG CTTGATAAGC TTGGCTGTTT TGGCGGATGA GAGAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG AATTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG AAACGCCGTA GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAATTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTTG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTT GAACAACACT CAACCCTATC TCGGGCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT TACAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATAGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC AAGGAGATGG CGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCTGCTCAT GTTTGACAGC TTATC
Details for BaquA.17156.a.DG13.PD00386
| PURIFICATION DATe: | 7/15/2015 |
| CONCENTRATION: | 9.8mg/ml |
| OBSERVED MW: | 41kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25mM TRIS pH 8.0, 200mM NaCl, 1mM TCEP 1% glycerol |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
This is Beryllium Batch BOS040100
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSL STRIAPHLPY LRRFARSVTG SQSSGDAYVS AMLEALVADI SIFPRASCDR IGTYWLFCHL FDQTTPNIPE PLPQFGLEQK TSAKLSYLTP RARQAFLLIA VEGFNEQEAS EIMNLDARDF RKLLNQASID ISQQIATQVM IIEDEPLIAM DIEQMVESLG HQVVGIARTR KEAVVMYHQK KPRLILAAIQ LADNSSGIDA VNDILQNDRI PVIFITAFPE RLLTGERPEP TFLVTKPFNP DMVKALISQA LFFKENASKA A
Full NT Sequence (Expression Vector + Insert)
ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC ATGTCTCTGA GCACTCGTAT CGCTCCGCAC CTGCCGTACT TGCGTCGTTT CGCTAGATCT GTGACCGGTT CTCAGAGCTC CGGTGATGCG TACGTCTCTG CCATGCTTGA GGCTTTAGTC GCAGACATCT CTATCTTTCC GCGTGCATCG TGTGACCGCA TAGGCACTTA CTGGCTGTTT TGCCACTTGT TCGATCAGAC TACTCCGAAC ATTCCGGAAC CGCTCCCGCA GTTTGGTCTG GAACAGAAAA CTAGCGCAAA ACTGAGCTAC CTGACCCCGC GCGCGCGTCA GGCATTCCTG CTGATCGCTG TTGAAGGTTT CAACGAACAG GAAGCATCTG AAATCATGAA CCTGGACGCG CGTGATTTCC GTAAACTGCT GAACCAGGCG TCTATTGACA TCTCCCAGCA GATCGCTACT CAGGTTATGA TTATCGAAGA CGAACCGTTA ATCGCAATGG ACATTGAACA GATGGTTGAA TCTCTGGGTC ACCAGGTTGT AGGTATCGCT CGTACTCGTA AAGAAGCTGT AGTTATGTAC CACCAGAAGA AGCCGCGCCT TATTCTTGCT GCTATCCAGT TAGCTGACAA CTCCTCCGGT ATCGACGCTG TCAACGATAT CCTGCAGAAC GACCGTATCC CGGTAATCTT TATCACCGCT TTTCCGGAAC GTCTGCTGAC CGGTGAACGC CCAGAACCGA CCTTCCTGGT CACAAAGCCG TTTAACCCGG ATATGGTTAA AGCTCTGATC TCCCAGGCAC TGTTCTTCAA AGAAAACGCT TCCAAAGCTG CTTGATAAGC TTGGCTGTTT TGGCGGATGA GAGAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG AATTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG AAACGCCGTA GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAATTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTTG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTT GAACAACACT CAACCCTATC TCGGGCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT TACAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATAGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC AAGGAGATGG CGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCTGCTCAT GTTTGACAGC TTATC
Details for BaquA.17156.a.DG14.PD00387
| PURIFICATION DATe: | 7/15/2015 |
| CONCENTRATION: | 10.2mg/ml |
| OBSERVED MW: | 41kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25mM TRIS pH 8.0, 200mM NaCl, 1mM TCEP 1% glycerol |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
This is Beryllium Batch BOS040107
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSL STRIAPHLPY LRRFARSVTG SQSSGDAYVS AMLEALVADI SIFPRASCDR IGTYWLFCHL FDQTTPNIPE PLPQFGLEQK TSAKLSYLTP RARQAFLLIA VEGFNEQEAS EIMNLDARDF RKLLNQASID ISQQIATQVM IIEDEPLIAM DIEQMVESLG HQVVGIARTR KEAVVMYHQK KPRLILADIQ LAANSSGIDA VNDILQNDRI PVIFITAFPE RLLTGERPEP TFLVTKPFNP DMVKALISQA LFFKENASKA A
Full NT Sequence (Expression Vector + Insert)
ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC ATGTCTCTGA GCACTCGTAT CGCTCCGCAC CTGCCGTACT TGCGTCGTTT CGCTAGATCT GTGACCGGTT CTCAGAGCTC CGGTGATGCG TACGTCTCTG CCATGCTTGA GGCTTTAGTC GCAGACATCT CTATCTTTCC GCGTGCATCG TGTGACCGCA TAGGCACTTA CTGGCTGTTT TGCCACTTGT TCGATCAGAC TACTCCGAAC ATTCCGGAAC CGCTCCCGCA GTTTGGTCTG GAACAGAAAA CTAGCGCAAA ACTGAGCTAC CTGACCCCGC GCGCGCGTCA GGCATTCCTG CTGATCGCTG TTGAAGGTTT CAACGAACAG GAAGCATCTG AAATCATGAA CCTGGACGCG CGTGATTTCC GTAAACTGCT GAACCAGGCG TCTATTGACA TCTCCCAGCA GATCGCTACT CAGGTTATGA TTATCGAAGA CGAACCGTTA ATCGCAATGG ACATTGAACA GATGGTTGAA TCTCTGGGTC ACCAGGTTGT AGGTATCGCT CGTACTCGTA AAGAAGCTGT AGTTATGTAC CACCAGAAGA AGCCGCGCCT TATTCTTGCT GACATCCAGT TAGCTGCTAA CTCCTCCGGT ATCGACGCTG TCAACGATAT CCTGCAGAAC GACCGTATCC CGGTAATCTT TATCACCGCT TTTCCGGAAC GTCTGCTGAC CGGTGAACGC CCAGAACCGA CCTTCCTGGT CACAAAGCCG TTTAACCCGG ATATGGTTAA AGCTCTGATC TCCCAGGCAC TGTTCTTCAA AGAAAACGCT TCCAAAGCTG CTTGATAAGC TTGGCTGTTT TGGCGGATGA GAGAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG AATTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG AAACGCCGTA GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAATTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTTG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTT GAACAACACT CAACCCTATC TCGGGCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT TACAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATAGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC AAGGAGATGG CGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCTGCTCAT GTTTGACAGC TTATC