BaquA.01676.a

Histidine kinase (EC 2.7.13.3)

CENTER ID: BaquA.01676.a
ORGANISM: Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.01676.a.B3.GE39454 includes HisKA HATPase_c 189 446
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283165.4.peg.386
RefSeq: WP_011179132.1
UniProt: A0A0H3M2D8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIEPSKKFVR WLTKKMPKRL YARSLIIIIA PMVLLQTVIA YVFMERHWQM VTERLSTAVV HDISAIIDII ETYPQQDNYE DIKRIAQQRM GLNISLLPPA PLPPPGPKPF FAILDYFLSE EITRQINRPF WIDTVGDSDL VEIRIHLGHN ILRVFAPRSQ AYASNTGIFL SWMVGTALVL LLIAIYFLRN QIKPIQQLAE AAESFGRGRP LPEGFQPQGA DEVRRAGIAF LRMRERIERQ IEQRTMMLSG VSHDLRTILT RFKLQLALAN TDFDTNLLEQ DVSDMQNMLE DYLAFARGEG SESVKTFDLK ALMQKFSADA HLHKRQFSYI IEGPTEIQVR PHAFTRLVNN LVSNAFCYGN TVKLTATSQQ ESFILTIDDD GPGIHKTMRT EVFKPFFRLD KARNQDASGT GLGLSIAQDI ARSHGGNIQL DKSPLGGLRA IIDIPL
NT Sequence
atgatcgaac cctcaaaaaa atttgtccga tggttaacca agaagatgcc taaacggctt tatgcccgtt ctttgatcat tattattgct cctatggtac tcctgcaaac agtaattgcg tacgtcttta tggaacgcca ttggcaaatg gtaaccgagc gtctttcaac cgctgttgtt catgatattt cggccatcat tgatatcatt gaaacatatc cacaacaaga taactatgaa gatatcaagc gtattgcaca acaacgcatg ggattaaaca tttctcttct accacctgct ccccttcccc ctccaggacc aaaaccattt tttgcaattc ttgattattt tttaagtgaa gaaatcactc gccaaattaa ccgccctttc tggattgata cagtgggaga ttctgatctt gtcgaaatcc gcatccacct tggtcacaac atcttgcgcg tctttgcacc acgcagccaa gcctatgctt ccaataccgg tattttttta agttggatgg taggaacagc tcttgtttta ttacttatag cgatttactt cttacgcaac caaatcaaac cgattcaaca attggctgaa gcagcagaaa gttttgggcg cggtcgtcct ttaccagaag gatttcaacc acaaggagct gatgaagtcc gtcgtgctgg cattgctttt ttacgcatgc gtgaacgtat tgaacgtcaa atcgaacaac gcaccatgat gctttcgggc gtaagccatg atttaagaac cattctcaca cgttttaaac ttcagctagc cttagcaaat actgactttg atactaatct gcttgagcaa gatgtaagcg acatgcaaaa tatgttagaa gactatcttg cttttgcccg cggagaaggc agtgaaagtg ttaaaacttt tgatttaaag gctcttatgc aaaaattctc cgccgatgct catctccaca aacgtcaatt ttcctatatc attgaaggcc ctacagagat acaagtacgc ccacatgcct tcacccgcct cgtcaacaat cttgtatcaa acgcattttg ttacggcaat accgtcaaac taacagccac atctcagcaa gaatctttta tattgacaat cgacgatgat ggacctggaa ttcataaaac tatgcgtaca gaagtcttta agccgttctt tagactcgat aaagcacgaa atcaagacgc aagtggaaca gggcttggac tttctattgc ccaagatata gcacgtagcc atggtggcaa cattcaatta gataagagcc ctctcggggg cttgcgcgct attattgata tccccctata a
Details for BaquA.01676.a.B3.GE39454
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 30kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR NQIKPIQQLA EAAESFGRGR PLPEGFQPQG ADEVRRAGIA FLRMRERIER QIEQRTMMLS GVSHDLRTIL TRFKLQLALA NTDFDTNLLE QDVSDMQNML EDYLAFARGE GSESVKTFDL KALMQKFSAD AHLHKRQFSY IIEGPTEIQV RPHAFTRLVN NLVSNAFCYG NTVKLTATSQ QESFILTIDD DGPGIHKTMR TEVFKPFFRL DKARNQDASG TGLGLSIAQD IARSHGGNIQ LDKSPLGGLR AIIDIPL
Validated NT Sequence
atcttactca cttatagggg gatatcaata atagcgcgca agcccccgag agggctctta tctaattgaa tgttgccacc atggctacgt gctatatctt gggcaataga aagtccaagc cctgttccac ttgcgtcttg atttcgtgct ttatcgagtc taaagaacgg cttaaagact tctgtacgca tagttttatg aattccaggt ccatcatcgt cgattgtcaa tataaaagat tcttgctgag atgtggctgt tagtttgacg gtattgccgt aacaaaatgc gtttgataca agattgttga cgaggcgggt gaaggcatgt gggcgtactt gtatctctgt agggccttca atgatatagg aaaattgacg tttgtggaga tgagcatcgg cggagaattt ttgcataaga gcctttaaat caaaagtttt aacactttca ctgccttctc cgcgggcaaa agcaagatag tcttctaaca tattttgcat gtcgcttaca tcttgctcaa gcagattagt atcaaagtca gtatttgcta aggctagctg aagtttaaaa cgtgtgagaa tggttcttaa atcatggctt acgcccgaaa gcatcatggt gcgttgttcg atttgacgtt caatacgttc acgcatgcgt aaaaaagcaa tgccagcacg acggacttca tcagctcctt gtggttgaaa tccttctggt aaaggacgac cgcgcccaaa actttctgct gcttcagcca attgttgaat cggtttgatt tggttgcgca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHRN QIKPIQQLAE AAESFGRGRP LPEGFQPQGA DEVRRAGIAF LRMRERIERQ IEQRTMMLSG VSHDLRTILT RFKLQLALAN TDFDTNLLEQ DVSDMQNMLE DYLAFARGEG SESVKTFDLK ALMQKFSADA HLHKRQFSYI IEGPTEIQVR PHAFTRLVNN LVSNAFCYGN TVKLTATSQQ ESFILTIDDD GPGIHKTMRT EVFKPFFRLD KARNQDASGT GLGLSIAQDI ARSHGGNIQL DKSPLGGLRA IIDIPL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcaacc aaatcaaacc gattcaacaa ttggctgaag cagcagaaag ttttgggcgc ggtcgtcctt taccagaagg atttcaacca caaggagctg atgaagtccg tcgtgctggc attgcttttt tacgcatgcg tgaacgtatt gaacgtcaaa tcgaacaacg caccatgatg ctttcgggcg taagccatga tttaagaacc attctcacac gttttaaact tcagctagcc ttagcaaata ctgactttga tactaatctg cttgagcaag atgtaagcga catgcaaaat atgttagaag actatcttgc ttttgcccgc ggagaaggca gtgaaagtgt taaaactttt gatttaaagg ctcttatgca aaaattctcc gccgatgctc atctccacaa acgtcaattt tcctatatca ttgaaggccc tacagagata caagtacgcc cacatgcctt cacccgcctc gtcaacaatc ttgtatcaaa cgcattttgt tacggcaata ccgtcaaact aacagccaca tctcagcaag aatcttttat attgacaatc gacgatgatg gacctggaat tcataaaact atgcgtacag aagtctttaa gccgttcttt agactcgata aagcacgaaa tcaagacgca agtggaacag ggcttggact ttctattgcc caagatatag cacgtagcca tggtggcaac attcaattag ataagagccc tctcgggggc ttgcgcgcta ttattgatat ccccctatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at