BaquA.01671.a

Histidine kinase (EC 2.7.13.3)

CENTER ID: BaquA.01671.a
ORGANISM: Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.01671.a.B2.GE39453 includes HisKA HATPase_c 75 814
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.01671.a.A3.PW37827 HisKA HATPase_c 590 814

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283165.4.peg.34
RefSeq: WP_011178864.1
UniProt: A0A0H3M1Q2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSFGDYDPK EKAQKMTRIF IDIFCLLFFF FPTCATAQSL EAHLPSYFTN GPWLLLALCG GVSCASLLIC MAVIMHAKKA SQKFLKTIQA DFSEKLKYYE WLLEETGQYL LIWETPTALP HVIGNIPALQ KAGIPQQNFQ CFELWLEETS LLELDHALTQ LRCKSHPFEL SITTKNNMLL QVTGVIAGTV AIARFQDTST QQSENARLQK DITRILTELR MQRNLLDLIQ EPVWIQDCEG KVCFINRAFR EMTGFREDSN KVVDLFNETT QRQTDETETI FQEHVHTVIG GERYRFHLTR ITTTEGMAAF ARDDSAYENL SNELRRVLQS HCETLDQIST AVAIFDTNQK LKFCNHAFKI LWPLENSFLE SEPSHTLLLE RLREKGLIGE NPDWRTWKEE LFKAYRQTES NQQIWNLPDG RTVRVISSPH PQGGVTWLYE NLTEKIDLER RYNTLIKIQG ETLDKLSEGV VVFATDGRLR LSNPALSKLW SLPYNLLVEG THITQLQSYC SPLTVGKEWD RFTKFITGFA EKRDTYSGRM DLKNGMVIDY TLVPLPNGET MLTFVNVTDT IYVARALQEK NETLESADRL RNEFVQHVSY ELRTPLTNII GFSDILRDQI FGSINERQKE YLGHIHSESS TLLNIVNDIL DLATLDAGIM ELNIKSVNIA DAMVQAVARV EERLNGRHIT LLQQISPSLN FISADATRLH QIFVNILSNA INFATEASTI EFCAEEQDNN IVFSVHNEGS DIPKDILDRI FKRFSSHSHH GGRAGAGLGL SLVKSFVELH NGHVEILTGA GQGTTVQCFF PIPK
NT Sequence
gtgctcagct ttggtgacta cgaccccaaa gaaaaagctc aaaaaatgac ccgaatattt atcgatattt tttgccttct tttctttttt tttccgacat gcgctactgc tcaatcattg gaagctcatt taccatctta tttcaccaat ggtccatggc ttcttttagc actctgtgga ggagtttctt gtgcttcact tctcatttgc atggcagtta ttatgcatgc aaaaaaagct tcacagaagt ttttgaaaac aattcaagca gatttttctg aaaaacttaa gtattacgaa tggctcctgg aagaaaccgg ccagtatctt cttatttggg agacccccac ggctttacct catgtgattg gcaatattcc agcgttgcaa aaagcaggaa ttcctcaaca aaattttcag tgttttgaac tctggcttga agaaacttct ctgctggaac ttgaccacgc attaacgcaa ttacgttgca aatctcaccc ttttgagctc tctatcacca caaagaataa tatgttgtta caagttacag gagtcatcgc aggaacagta gccattgcac gctttcaaga tacctcaaca cagcagagcg aaaatgctcg tttacagaaa gatatcaccc gaatccttac agaattaaga atgcagcgca acctccttga tcttattcaa gaaccagtat ggatacaaga ttgtgaaggg aaagtttgtt ttatcaatcg cgcctttaga gaaatgacag gttttcgaga agacagtaat aaagtagttg atcttttcaa tgaaacaaca caacgtcaaa cagatgaaac agaaacaatc tttcaagagc acgttcatac ggtcatcggt ggagaacggt atcgttttca tctcacacgg attacaacca ctgaagggat ggcagccttt gcacgtgatg acagtgcata tgaaaatctt tccaatgaat taagacgtgt ccttcaaagc cattgtgaaa cacttgatca aatctcaact gctgtagcta tttttgatac caaccaaaaa ttaaaattct gcaaccatgc gtttaaaatt ttatggccgt tggagaactc ttttctagaa agtgaaccaa gtcatacatt gctccttgaa cgcttgcgtg aaaaagggct tattggtgaa aatcctgatt ggcgcacatg gaaagaagaa ctttttaaag cctaccggca aacagaatca aatcaacaaa tttggaatct gccagatggg cgcacagtgc gtgttatttc tagtcctcat ccacaaggag gtgtcacttg gctttacgaa aatctcacag aaaaaattga ccttgaacga cgctataata cactcattaa aatacagggc gaaacacttg ataagctttc agaaggagtg gtggtttttg ctactgacgg acgccttcgt ttatccaatc ctgcgttatc gaaattatgg tcactaccct ataatttatt ggtggaaggc acccatatta cgcagttaca aagttattgt tcacccctca ctgtggggaa agaatgggat cggtttacta aattcattac tggttttgct gaaaaacgtg atacatattc aggtcgtatg gacctcaaaa atggcatggt cattgattat acgttggtcc cccttcccaa tggagagacg atgctcactt tcgtaaatgt gacagatact atttatgtcg cgcgtgccct tcaagagaaa aatgaaacct tagaaagcgc tgatcgtttg cgcaatgaat ttgtccaaca tgtttcctat gaattgcgta cgcctcttac caatattatt ggattttccg atattttacg cgatcaaatc tttggctcta tcaatgaacg ccaaaaggaa tatcttggcc atattcactc agaatcaagc actcttttga atattgttaa tgatattctt gaccttgcaa cccttgatgc aggcataatg gagcttaaca taaaatcagt taatattgct gatgctatgg tgcaagccgt agcgcgcgtg gaagagcgtc ttaacggacg tcatatcacg cttttacaac aaatttctcc ttccttgaat tttatttctg cggatgcaac ccgtttgcat caaatctttg tgaatatcct cagcaatgcc attaattttg caacggaagc aagtacgatt gaattttgtg ctgaagaaca agataataat atcgtcttca gtgtccacaa tgagggatct gatattccaa aagacatcct tgatcgaatt ttcaaacgtt tttcttccca ttcacatcat ggggggcgtg caggagcagg tcttggtctc tcccttgtca aaagctttgt tgaactccac aatggacatg ttgaaattct tactggtgct ggacaaggaa caacggttca atgttttttt ccaattccca aatga
Details for BaquA.01671.a.B2.GE39453
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 85kDa
OBSERVED MW: 85kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMH AKKASQKFLK TIQADFSEKL KYYEWLLEET GQYLLIWETP TALPHVIGNI PALQKAGIPQ QNFQCFELWL EETSLLELDH ALTQLRCKSH PFELSITTKN NMLLQVTGVI AGTVAIARFQ DTSTQQSENA RLQKDITRIL TELRMQRNLL DLIQEPVWIQ DCEGKVCFIN RAFREMTGFR EDSNKVVDLF NETTQRQTDE TETIFQEHVH TVIGGERYRF HLTRITTTEG MAAFARDDSA YENLSNELRR VLQSHCETLD QISTAVAIFD TNQKLKFCNH AFKILWPLEN SFLESEPSHT LLLERLREKG LIGENPDWRT WKEELFKAYR QTESNQQIWN LPDGRTVRVI SSPHPQGGVT WLYENLTEKI DLERRYNTLI KIQGETLDKL SEGVVVFATD GRLRLSNPAL SKLWSLPYNL LVEGTHITQL QSYCSPLTVG KEWDRFTKFI TGFAEKRDTY SGRMDLKNGM VIDYTLVPLP NGETMLTFVN VTDTIYVARA LQEKNETLES ADRLRNEFVQ HVSYELRTPL TNIIGFSDIL RDQIFGSINE RQKEYLGHIH SESSTLLNIV NDILDLATLD AGIMELNIKS VNIADAMVQA VARVEERLNG RHITLLQQIS PSLNFISADA TRLHQIFVNI LSNAINFATE ASTIEFCAEE QDNNIVFSVH NEGSDIPKDI LDRIFKRFSS HSHHGGRAGA GLGLSLVKSF VELHNGHVEI LTGAGQGTTV QCFFPIPK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatg caaaaaaagc ttcacagaag tttttgaaaa caattcaagc agatttttct gaaaaactta agtattacga atggctcctg gaagaaaccg gccagtatct tcttatttgg gagaccccca cggctttacc tcatgtgatt ggcaatattc cagcgttgca aaaagcagga attcctcaac aaaattttca gtgttttgaa ctctggcttg aagaaacttc tctgctggaa cttgaccacg cattaacgca attacgttgc aaatctcacc cttttgagct ctctatcacc acaaagaata atatgttgtt acaagttaca ggagtcatcg caggaacagt agccattgca cgctttcaag atacctcaac acagcagagc gaaaatgctc gtttacagaa agatatcacc cgaatcctta cagaattaag aatgcagcgc aacctccttg atcttattca agaaccagta tggatacaag attgtgaagg gaaagtttgt tttatcaatc gcgcctttag agaaatgaca ggttttcgag aagacagtaa taaagtagtt gatcttttca atgaaacaac acaacgtcaa acagatgaaa cagaaacaat ctttcaagag cacgttcata cggtcatcgg tggagaacgg tatcgttttc atctcacacg gattacaacc actgaaggga tggcagcctt tgcacgtgat gacagtgcat atgaaaatct ttccaatgaa ttaagacgtg tccttcaaag ccattgtgaa acacttgatc aaatctcaac tgctgtagct atttttgata ccaaccaaaa attaaaattc tgcaaccatg cgtttaaaat tttatggccg ttggagaact cttttctaga aagtgaacca agtcatacat tgctccttga acgcttgcgt gaaaaagggc ttattggtga aaatcctgat tggcgcacat ggaaagaaga actttttaaa gcctaccggc aaacagaatc aaatcaacaa atttggaatc tgccagatgg gcgcacagtg cgtgttattt ctagtcctca tccacaagga ggtgtcactt ggctttacga aaatctcaca gaaaaaattg accttgaacg acgctataat acactcatta aaatacaggg cgaaacactt gataagcttt cagaaggagt ggtggttttt gctactgacg gacgccttcg tttatccaat cctgcgttat cgaaattatg gtcactaccc tataatttat tggtggaagg cacccatatt acgcagttac aaagttattg ttcacccctc actgtgggga aagaatggga tcggtttact aaattcatta ctggttttgc tgaaaaacgt gatacatatt caggtcgtat ggacctcaaa aatggcatgg tcattgatta tacgttggtc ccccttccca atggagagac gatgctcact ttcgtaaatg tgacagatac tatttatgtc gcgcgtgccc ttcaagagaa aaatgaaacc ttagaaagcg ctgatcgttt gcgcaatgaa tttgtccaac atgtttccta tgaattgcgt acgcctctta ccaatattat tggattttcc gatattttac gcgatcaaat ctttggctct atcaatgaac gccaaaagga atatcttggc catattcact cagaatcaag cactcttttg aatattgtta atgatattct tgaccttgca acccttgatg caggcataat ggagcttaac ataaaatcag ttaatattgc tgatgctatg gtgcaagccg tagcgcgcgt ggaagagcgt cttaacggac gtcatatcac gcttttacaa caaatttctc cttccttgaa ttttatttct gcggatgcaa cccgtttgca tcaaatcttt gtgaatatcc tcagcaatgc cattaatttt gcaacggaag caagtacgat tgaattttgt gctgaagaac aagataataa tatcgtcttc agtgtccaca atgagggatc tgatattcca aaagacatcc ttgatcgaat tttcaaacgt ttttcttccc attcacatca tggggggcgt gcaggagcag gtcttggtct ctcccttgtc aaaagctttg ttgaactcca caatggacat gttgaaattc ttactggtgc tggacaagga acaacggttc aatgtttttt tccaattccc aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BaquA.01671.a.A3.PW37827
PURIFICATION DATe: 12/15/2015
CONCENTRATION: 46mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 23
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLRNEFVQH VSYELRTPLT NIIGFSDILR DQIFGSINER QKEYLGHIHS ESSTLLNIVN DILDLATLDA GIMELNIKSV NIADXMVQAV ARVEERLNGR HITLLQQISP SLNFISADAT RLHQIFVNIL SNAINFATEA STIEFCAEEQ DNNIVFSVHN EGSDIPKDIL DRIFKRFSSH SHHGGRAGAG LGLSLVKSFV ELHNGHVEIL TGAGQGTTVQ CFFPIPK
Validated NT Sequence
attatttggg aattggaaaa aaacattgaa ccgttgttcc ttgtccagca ccagtaagaa tttcaacatg tccattgtgg agttcaacaa agcttttgac aagggagaga ccaagacctg ctcctgcacg ccccccatga tgtgaatggg aagaaaaacg tttgaaaatt cgatcaagga tgtcttttgg aatatcagat ccctcattgt ggacactgaa gacgatatta ttatcttgtt cttcagcaca aaattcaatc gtacttgctt ccgttgcaaa attaatggca ttgctgagga tattcacaaa gatttgatgc aaacgggttg catccgcaga aataaaattc aaggaaggag aaatttgttg taaaagcgtg atatgacgtc cgttaagacg ctcttccacg cgcgctacgg cttgcaccat annatcagca atattaactg attttatgtt aagctccatt atgcctgcat caagggttgc aaggtcaaga atatcattaa caatattcaa aagagtgctt gattctgagt gaatatggcc aagatattcc ttttggcgtt cattgataga gccaaagatt tgatcgcgta aaatatcgga aaatccaata atattggtaa gaggcgtacg caattcatag gaaacatgtt ggacaaattc attgcgcaac atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SLRNEFVQHV SYELRTPLTN IIGFSDILRD QIFGSINERQ KEYLGHIHSE SSTLLNIVND ILDLATLDAG IMELNIKSVN IADAMVQAVA RVEERLNGRH ITLLQQISPS LNFISADATR LHQIFVNILS NAINFATEAS TIEFCAEEQD NNIVFSVHNE GSDIPKDILD RIFKRFSSHS HHGGRAGAGL GLSLVKSFVE LHNGHVEILT GAGQGTTVQC FFPIPK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGTT GCGCAATGAA TTTGTCCAAC ATGTTTCCTA TGAATTGCGT ACGCCTCTTA CCAATATTAT TGGATTTTCC GATATTTTAC GCGATCAAAT CTTTGGCTCT ATCAATGAAC GCCAAAAGGA ATATCTTGGC CATATTCACT CAGAATCAAG CACTCTTTTG AATATTGTTA ATGATATTCT TGACCTTGCA ACCCTTGATG CAGGCATAAT GGAGCTTAAC ATAAAATCAG TTAATATTGC TGATGCTATG GTGCAAGCCG TAGCGCGCGT GGAAGAGCGT CTTAACGGAC GTCATATCAC GCTTTTACAA CAAATTTCTC CTTCCTTGAA TTTTATTTCT GCGGATGCAA CCCGTTTGCA TCAAATCTTT GTGAATATCC TCAGCAATGC CATTAATTTT GCAACGGAAG CAAGTACGAT TGAATTTTGT GCTGAAGAAC AAGATAATAA TATCGTCTTC AGTGTCCACA ATGAGGGATC TGATATTCCA AAAGACATCC TTGATCGAAT TTTCAAACGT TTTTCTTCCC ATTCACATCA TGGGGGGCGT GCAGGAGCAG GTCTTGGTCT CTCCCTTGTC AAAAGCTTTG TTGAACTCCA CAATGGACAT GTTGAAATTC TTACTGGTGC TGGACAAGGA ACAACGGTTC AATGTTTTTT TCCAATTCCC AAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA