BaquA.01669.b

Histidine kinase (EC 2.7.13.3)

CENTER ID: BaquA.01669.b
ORGANISM: Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.01669.b.B1.GE39452 full length 1 435
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283165.4.peg.328
UniProt: A0A0H3LTI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQEKVQNKRD EKLDTKISGL SELEHSAFRE IAERLRGELE VAGAHLVEET AVQLPCAQTL SGNIIEQAFI KEPAAVLSLL DTATDGILWL NEQGFIQSVS RAVLALTGYE INELRRQPLS SLFTLQSRSL IEKYFKLITM KGKNQVFTRG EIADLVTKSS KNITVSMTIV SLAQQNSYAV MLSDMTDVML SLEKKVEENK MVGVVHEMRT PLNALIGFAE IMRDGRFGSI ENERYRGYLR DIISSGKHIL SLVNQLLECS KANHSGSKNQ INTPIIAETF DVISCLRASM AFLETQANHN GIIMRIIAPV HVPFICVSQQ IFRQIIWNLL SNAIRFTPSG GQIIVHVSYG KKECVKISVS DNGVGMSDEE IVHALQPYGQ VERKDRHSGD SVFVGTGLGL PICKAMVEES GGQFLLFSKP NHGTTVEMFF PVFHG
NT Sequence
atgcaagaaa aagtacaaaa caaaagagat gaaaagctgg atacaaaaat atcgggtctc tcggagctag agcattcagc ttttcgggag attgcagagc gattacgtgg cgaattggag gtagcggggg cacatttggt tgaagaaaca gcggttcagt taccatgtgc acagacattg tctggaaata tcatagaaca agctttcata aaagaaccgg cagcggtttt atcattgttg gatacagcta ctgatggcat tttgtggttg aatgaacagg gcttcattca gtcggttagt cgtgctgttt tagcattaac tggctatgaa ataaatgaat tacggagaca gccattatca tcattattta ctttgcaaag ccgatctttg attgagaaat attttaagtt gattactatg aaaggaaaaa atcaggtttt tactcgcggt gagatagctg atttagtgac gaaaagttct aagaatataa ccgtttctat gacgattgta tctttagcac aacaaaatag ttatgctgtt atgctgtccg atatgacaga tgtgatgctg tccttagaga aaaaggtaga agaaaataaa atggttggag ttgttcatga aatgcggaca cctttgaatg ctcttatcgg ttttgcagaa attatgaggg atggtcgttt tggttcgatt gaaaatgagc gttatcgcgg atatttacgc gatattatct catctggaaa acatatcttg tcgctggtga atcaattact agagtgctcg aaagcaaatc attctggctc aaagaatcag atcaatacgc ccattatagc tgaaacattt gatgtgatat cctgtttacg tgcgagtatg gcttttcttg aaacacaagc gaatcataat ggcattatca tgcgcattat tgcaccggtt catgtaccat ttatttgtgt atcgcaacag atatttcggc aaattatatg gaatctcctt tccaatgcta tccgttttac accatcaggt gggcaaatta ttgttcacgt ttcttatggt aaaaaagagt gcgtgaagat ttcagtgagt gataatggtg tagggatgag cgacgaagag attgtgcacg ctctacaacc ttatggtcag gtagaacgca aagatcgcca ctctggtgac agcgtttttg tcggaactgg tctaggacta ccgatatgta aagcaatggt agaagaaagt ggtggtcaat ttttgttgtt ttcaaaacct aatcatggaa caactgtgga aatgtttttt cctgtgtttc atgggtga
Details for BaquA.01669.b.B1.GE39452
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 49kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MQEKVQNKRD EKLDTKISGL SELEHSAFRE IAERLRGELE VAGAHLVEET AVQLSCAQTL SGNIIEQAFI KEPAAVLSLL DTATDGILWL NEQGFIQSVS RAVLALTGYE INELRRQPLS SLFTLQSRSL IEKYFKLITM KGKNQVFTRG EIADLVTKSS KNITVSMTIV SLAQQNSYAV MLSDMTDVML SLEKKVEENK MVGVVHEMRT PLNALIGFAE IMRDGRFGSI ENERYRGYLR DIISSGKHIL SLVNQLLECS KANHSGSKNQ INTPIIAETF DVISCLRASM TFLETQANHN GIIMRIIAPV HVPFICVSQQ IFRQIIWNLL SNAIRFTPSG GQIIVHVSYG KKECVKISVS DNGVGMSDEE IVHALQPYGQ VERKDRHSGD SVFVGTGLGL PICKAMVEES GGQFLLFSKP NHGTTV
Validated NT Sequence
ttccacagtt gttccatgat taggttttga aaacaacaaa aattgaccac cactttcttc taccattgct ttacatatcg gtagtcctag accagttccg acaaaaacgc tgtcaccaga gtggcgatct ttgcgttcta cctgaccata aggttgtaga gcgtgcacaa tctcttcgtc gctcatccct acaccattat cactcactga aatcttcacg cactcttttt taccataaga aacgtgaaca ataatttgcc cacctgatgg tgtaaaacgg atagcattgg aaaggagatt ccatataatt tgccgaaata tctgttgcga tacacaaata aatggtacat gaaccggtgc aataatgcgc atgataatgc cattatgatt cgcttgtgtt tcaagaaaag tcatactcgc acgtaaacag gatatcacat caaatgtttc agctataatg ggcgtattga tctgattctt tgagccagaa tgatttgctt tcgagcactc tagtaattga ttcaccagcg acaagatatg ttttccagat gagataatat cgcgtaaata tccgcgataa cgctcatttt caatcgaacc aaaacgacca tccctcataa tttctgcaaa accgataaga gcattcaaag gtgtccgcat ttcatgaaca actccaacca ttttattttc ttctaccttt ttctctaagg acagcatcac atctgtcata tcggacagca taacagcata actattttgt tgtgctaaag atacaatcgt catagaaacg gttatattct tagaactttt cgtcactaaa tcagctatct caccgcgagt aaaaacctga ttttttcctt tcatagtaat caacttaaaa tatttctcaa tcaaagatcg gctttgcaaa gtaaataatg atgataatgg ctgtctccgt aattcattta tttcatagcc agttaatgct aaaacagcac gactaaccga ctgaatgaag ccctgttcat tcaaccacaa aatgccatca gtagctgtat ccaacaatga taaaaccgct gccggttctt ttatgaaagc ttgttctatg atatttccag acaatgtctg tgcacatgat aactgaaccg ctgtttcttc aaccaaatgt gcccccgcta cctccaattc gccacgtaat cgctctgcaa tctcccgaaa agctgaatgc tctagctccg agagacccga tatttttgta tccagctttt catctctttt gttttgtact ttttcttgca tatggtggtg gtggtggtga
Expected Protein Sequence
MAHHHHHHMQ EKVQNKRDEK LDTKISGLSE LEHSAFREIA ERLRGELEVA GAHLVEETAV QLPCAQTLSG NIIEQAFIKE PAAVLSLLDT ATDGILWLNE QGFIQSVSRA VLALTGYEIN ELRRQPLSSL FTLQSRSLIE KYFKLITMKG KNQVFTRGEI ADLVTKSSKN ITVSMTIVSL AQQNSYAVML SDMTDVMLSL EKKVEENKMV GVVHEMRTPL NALIGFAEIM RDGRFGSIEN ERYRGYLRDI ISSGKHILSL VNQLLECSKA NHSGSKNQIN TPIIAETFDV ISCLRASMAF LETQANHNGI IMRIIAPVHV PFICVSQQIF RQIIWNLLSN AIRFTPSGGQ IIVHVSYGKK ECVKISVSDN GVGMSDEEIV HALQPYGQVE RKDRHSGDSV FVGTGLGLPI CKAMVEESGG QFLLFSKPNH GTTVEMFFPV FHG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag aaaaagtaca aaacaaaaga gatgaaaagc tggatacaaa aatatcgggt ctctcggagc tagagcattc agcttttcgg gagattgcag agcgattacg tggcgaattg gaggtagcgg gggcacattt ggttgaagaa acagcggttc agttaccatg tgcacagaca ttgtctggaa atatcataga acaagctttc ataaaagaac cggcagcggt tttatcattg ttggatacag ctactgatgg cattttgtgg ttgaatgaac agggcttcat tcagtcggtt agtcgtgctg ttttagcatt aactggctat gaaataaatg aattacggag acagccatta tcatcattat ttactttgca aagccgatct ttgattgaga aatattttaa gttgattact atgaaaggaa aaaatcaggt ttttactcgc ggtgagatag ctgatttagt gacgaaaagt tctaagaata taaccgtttc tatgacgatt gtatctttag cacaacaaaa tagttatgct gttatgctgt ccgatatgac agatgtgatg ctgtccttag agaaaaaggt agaagaaaat aaaatggttg gagttgttca tgaaatgcgg acacctttga atgctcttat cggttttgca gaaattatga gggatggtcg ttttggttcg attgaaaatg agcgttatcg cggatattta cgcgatatta tctcatctgg aaaacatatc ttgtcgctgg tgaatcaatt actagagtgc tcgaaagcaa atcattctgg ctcaaagaat cagatcaata cgcccattat agctgaaaca tttgatgtga tatcctgttt acgtgcgagt atggcttttc ttgaaacaca agcgaatcat aatggcatta tcatgcgcat tattgcaccg gttcatgtac catttatttg tgtatcgcaa cagatatttc ggcaaattat atggaatctc ctttccaatg ctatccgttt tacaccatca ggtgggcaaa ttattgttca cgtttcttat ggtaaaaaag agtgcgtgaa gatttcagtg agtgataatg gtgtagggat gagcgacgaa gagattgtgc acgctctaca accttatggt caggtagaac gcaaagatcg ccactctggt gacagcgttt ttgtcggaac tggtctagga ctaccgatat gtaaagcaat ggtagaagaa agtggtggtc aatttttgtt gttttcaaaa cctaatcatg gaacaactgt ggaaatgttt tttcctgtgt ttcatgggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat