SSGCID: Target Page

BaquA.01669.a

Sensory transduction histidine kinase

CENTER ID:	BaquA.01669.a
ORGANISM:	Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.01669.a.B1.GE39202	full length		1	337

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.01669.a.D2.PW37864	HHpred domain, includes HWE_HK		124	336

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283165.4.peg.1192
RefSeq:	WP_011179754.1
RefSeq:	YP_032652.1
UniProt:	A0A0H3M1D2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIVTPLPTPP MDKSHLSALM QAIRSADICV LYQTTNLVYL WAENLPKHLH SKWRVGCRDS DFFSLDLADN METIKLQVLA TGKMQTIEAR FEDMAKQVIW YKFSIDCHCN DNGEIVGIIT TGVDISALRR REQVLKILLR EVSHRSKNLL AIIQSIASQT ARYTESLKVF LRKFQGRLHS LSHSQDLITA SDWRGAQFRE LVHSQALGYL IKETERFSLE GVDPYLFPNA ALHIGLAFHE LIVNSLSFGA LSQEKGSVCV RCELHTNING TTQLLITWNE NFAAGTILSN ENKACFGSAV LEKIVPVSVN GSASLNLTEE GIVYCLYVPD NYFDIFF

NT Sequence

atgatcgtta cgcctcttcc aacgccacct atggataaat cccatttgag cgctctgatg caagcgatcc gtagtgctga tatttgtgtt ttatatcaga caacgaatct ggtctattta tgggctgaaa atcttcccaa gcatctgcac agtaaatggc gggtgggatg tcgtgacagt gatttttttt cgctcgatct tgcggacaat atggaaacaa tcaaattgca ggttttagcc acaggaaaaa tgcaaaccat tgaagcacgg tttgaggata tggccaaaca agtgatttgg tataaatttt ctattgattg ccattgcaac gataacggag aaattgttgg tatcatcacc accggagttg atatttcagc attacgccga cgtgagcaag tgctaaaaat cctccttcgc gaagtcagcc accgttctaa aaatcttctg gccatcattc aaagtattgc cagccaaacc gcacgctata ctgaatcgct taaggttttc ttacgcaaat ttcaaggacg gctccattct ctttcacatt ctcaagatct gattactgct tctgattggc gtggtgccca atttcgtgaa ttggttcatt cgcaagcttt gggctatttg ataaaagaaa cagaacgttt ttcacttgaa ggcgtggatc cttatctgtt tccgaatgcc gctttgcata ttggtttggc ttttcatgaa ctcattgtca attctctttc ttttggggcc ttatcacaag aaaagggaag tgtctgtgtg cgttgtgaat tgcacacgaa cataaatgga acaacacaac tccttatcac ttggaatgaa aattttgcag caggaaccat tctctctaac gaaaataaag cctgttttgg cagtgctgtc ctagaaaaga ttgtccctgt ttctgtcaat ggttcggctt ccttaaactt aacagaagaa gggattgttt attgcctgta tgttcctgac aattattttg acattttttt ttaa

Details for BaquA.01669.a.B1.GE39202

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/5/2015
EXPECTED MW:	39kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMI VTPLPTPPMD KSHLSALMQA IRSADICVLY QTTNLVYLWA ENLPKHLHSK WRVGCRDSDF FSLDLADNME TIKLQVLATG KMQTIEARFE DMAKQVIWYK FSIDCHCNDN GEIVGIITTG VDISALRRRE QVLKILLREV SHRSKNLLAI IQSIASQTAR YTESLKVFLR KFQGRLHSLS HSQDLITASD WRGAQFRELV HSQALGYLIK ETERFSLEGV DPYLFPNAAL HIGLAFHELI VNSLSFGALS QEKGSVCVRC ELHTNINGTT QLLITWNENF AAGTILSNEN KACFGSAVLE KIVPVSVNGS ASLNLTEEGI VYCLYVP

Validated NT Sequence

gtcaggaaca tacaggcaat aaacaatccc ttcttctgtt aagtttaagg aagccgaacc attgacagaa acagggacaa tcttttctag gacagcactg ccaaaacagg ctttattttc gttagagaga atggttcctg ctgcaaaatt ttcattccaa gtgataagga gttgtgttgt tccatttatg ttcgtgtgca attcacaacg cacacagaca cttccctttt cttgtgataa ggccccaaaa gaaagagaat tgacaatgag ttcatgaaaa gccaaaccaa tatgcaaagc ggcattcgga aacagataag gatccacgcc ttcaagtgaa aaacgttctg tttcttttat caaatagccc aaagcttgcg aatgaaccaa ttcacgaaat tgggcaccac gccaatcaga agcagtaatc agatcttgag aatgtgaaag agaatggagc cgtccttgaa atttgcgtaa gaaaacctta agcgattcag tatagcgtgc ggtttggctg gcaatacttt gaatgatggc cagaagattt ttagaacggt ggctgacttc gcgaaggagg atttttagca cttgctcacg tcggcgtaat gctgaaatat caactccggt ggtgatgata ccaacaattt ctccgttatc gttgcaatgg caatcaatag aaaatttata ccaaatcact tgtttggcca tatcctcaaa ccgtgcttca atggtttgca tttttcctgt ggctaaaacc tgcaatttga ttgtttccat attgtccgca agatcgagcg aaaaaaaatc actgtcacga catcccaccc gccatttact gtgcagatgc ttgggaagat tttcagccca taaatagacc agattcgttg tctgatataa aacacaaata tcagcactac ggatcgcttg catcagagcg ctcaaatggg atttatccat aggtggcgtt ggaagaggcg taacgatcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg ttacgcctct tccaacgcca cctatggata aatcccattt gagcgctctg atgcaagcga tccgtagtgc tgatatttgt gttttatatc agacaacgaa tctggtctat ttatgggctg aaaatcttcc caagcatctg cacagtaaat ggcgggtggg atgtcgtgac agtgattttt tttcgctcga tcttgcggac aatatggaaa caatcaaatt gcaggtttta gccacaggaa aaatgcaaac cattgaagca cggtttgagg atatggccaa acaagtgatt tggtataaat tttctattga ttgccattgc aacgataacg gagaaattgt tggtatcatc accaccggag ttgatatttc agcattacgc cgacgtgagc aagtgctaaa aatcctcctt cgcgaagtca gccaccgttc taaaaatctt ctggccatca ttcaaagtat tgccagccaa accgcacgct atactgaatc gcttaaggtt ttcttacgca aatttcaagg acggctccat tctctttcac attctcaaga tctgattact gcttctgatt ggcgtggtgc ccaatttcgt gaattggttc attcgcaagc tttgggctat ttgataaaag aaacagaacg tttttcactt gaaggcgtgg atccttatct gtttccgaat gccgctttgc atattggttt ggcttttcat gaactcattg tcaattctct ttcttttggg gccttatcac aagaaaaggg aagtgtctgt gtgcgttgtg aattgcacac gaacataaat ggaacaacac aactccttat cacttggaat gaaaattttg cagcaggaac cattctctct aacgaaaata aagcctgttt tggcagtgct gtcctagaaa agattgtccc tgtttctgtc aatggttcgg cttccttaaa cttaacagaa gaagggattg tttattgcct gtatgttcct gacaattatt ttgacatttt tttttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for BaquA.01669.a.D2.PW37864

PURIFICATION DATe:	2/29/2016
CONCENTRATION:	21mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	85

Protocol Notes

notes unavailable

Validated AA Sequence

MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG MDISALRRRE QVLKILLREV SHRSKNLLAI IQSIASQTAR YTESLKVFLR KFQGRLHSLS HSQDLITASD WRGAQFRELV HSQALGYLIK ETERFSLEGV DPYLFPNAAL HIGLAFHELI VNSLSFGALS QEKGSVCVRC ELHTNINGTT QLLITWNENF AAGTILSNEN KACFGSAVLE KIVPVSVNGS ASLNLTEEGI VYCLYVPDNY FDIF

Validated NT Sequence

gggccaccac caccaccacc atagcggcga agtaaaaccg gaagtgaagc cggagaccca catcaacctg aaggttagcg acggtagcag cgaaatcttc ttcaagatta agaagaccac cccgctgcgt cgcctgatgg aagcattcgc taaacgccag ggcaaggaaa tggattccct gcgctttctg tacgacggta tccgtattca ggcagaccag actccggaag atctggacat ggaagataac gacattatcg aagcacaccg tgaacaaatc ggtgggatgg atatttcagc attacgccga cgtgagcaag tgctaaaaat cctccttcgc gaagtcagcc accgttctaa aaatcttctg gccatcattc aaagtattgc cagccaaacc gcacgctata ctgaatcgct taaggttttc ttacgcaaat ttcaaggacg gctccattct ctttcacatt ctcaagatct gattactgct tctgattggc gtggtgccca atttcgtgaa ttggttcatt cgcaagcttt gggctatttg ataaaagaaa cagaacgttt ttcacttgaa ggcgtggatc cttatctgtt tccgaatgcc gctttgcata ttggtttggc ttttcatgaa ctcattgtca attctctttc ttttggggcc ttatcacaag aaaagggaag tgtctgtgtg cgttgtgaat tgcacacgaa cataaatgga acaacacaac tccttatcac ttggaatgaa aattttgcag caggaaccat tctctctaac gaaaataaag cctgttttgg cagtgctgtc ctagaaaaga ttgtccctgt ttctgtcaat ggttcggctt ccttaaactt aacagaagaa gggattgttt attgcctgta tgttcctgac aattattttg acatttttta g

Expressed Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGDISA LRRREQVLKI LLREVSHRSK NLLAIIQSIA SQTARYTESL KVFLRKFQGR LHSLSHSQDL ITASDWRGAQ FRELVHSQAL GYLIKETERF SLEGVDPYLF PNAALHIGLA FHELIVNSLS FGALSQEKGS VCVRCELHTN INGTTQLLIT WNENFAAGTI LSNENKACFG SAVLEKIVPV SVNGSASLNL TEEGIVYCLY VPDNYFDIF

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAGA TATTTCAGCA TTACGCCGAC GTGAGCAAGT GCTAAAAATC CTCCTTCGCG AAGTCAGCCA CCGTTCTAAA AATCTTCTGG CCATCATTCA AAGTATTGCC AGCCAAACCG CACGCTATAC TGAATCGCTT AAGGTTTTCT TACGCAAATT TCAAGGACGG CTCCATTCTC TTTCACATTC TCAAGATCTG ATTACTGCTT CTGATTGGCG TGGTGCCCAA TTTCGTGAAT TGGTTCATTC GCAAGCTTTG GGCTATTTGA TAAAAGAAAC AGAACGTTTT TCACTTGAAG GCGTGGATCC TTATCTGTTT CCGAATGCCG CTTTGCATAT TGGTTTGGCT TTTCATGAAC TCATTGTCAA TTCTCTTTCT TTTGGGGCCT TATCACAAGA AAAGGGAAGT GTCTGTGTGC GTTGTGAATT GCACACGAAC ATAAATGGAA CAACACAACT CCTTATCACT TGGAATGAAA ATTTTGCAGC AGGAACCATT CTCTCTAACG AAAATAAAGC CTGTTTTGGC AGTGCTGTCC TAGAAAAGAT TGTCCCTGTT TCTGTCAATG GTTCGGCTTC CTTAAACTTA ACAGAAGAAG GGATTGTTTA TTGCCTGTAT GTTCCTGACA ATTATTTTGA CATTTTTAGC TTGCGGCCGC ACTCGAGCAC CACCACCACC ACCACTGAGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGAT