BaquA.00863.c

Histidine kinase (EC 2.7.13.3)

CENTER ID: BaquA.00863.c
ORGANISM: Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.00863.c.B3.GE39451 includes HisKA HATPase_c 212 474
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaquA.00863.c.A4.PW37826 HisKA HATPase_c 260 471

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283165.4.peg.451
RefSeq: WP_011179180.1
UniProt: A0A0H3LVG4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIVLKNNSRL KNIFFLIGRS LSLRVMILST LWVIISLSSI SAVSILFYRR SSEQSLERIL FAQLYSLIAT VTVSSEGNLK GNLGIDDIRY SDPTSGWYWE VVAISSNLQG RLTSPSLGSR KIFSPSDVDI PFDNNFFRSY RIKGINDQKL QVIESDVVLD NQNHVARFRL VGNIDEAHAQ VQEFKRTLQI FLWSFGIGSV LINLVIILFS FQPLKLIRQA LNDIREGKVH YMSTDLVSEV MPLAQEMNTL IHNDQRIIER FRTQVGNLAH SLKTPLSVIM NEADKMCGEQ AILLREQTKI MQDQINHYLK RARIAAQRDS VVYHASVRKV VDRLVRVMKK LNPEKQFQFV MDVDDIIFSG EREDLEEVVG NLIENAAQWS RKKVLISCYL EKNIEEAEFF SILVEDDGFG LTEEQIDEAF KRGHRFDESK PGTGLGLAIV SDMVNEYGGS LFLSRSDLGG LYVRVLLPKK VTLL
NT Sequence
atgattgttc taaagaacaa tagccggctt aagaatatat tttttttaat cggtagatct ctcagcttac gcgttatgat cttatcaaca ttatgggtca ttatttcgct ttcatcgatt tctgcagtca gtattttgtt ttatcgacgc tcaagtgaac aaagcctaga gcgtatcctt tttgctcagc tttacagtct tattgcgaca gtgactgtgt caagtgaggg gaatttgaaa ggaaatttgg gaattgatga tattcgttat tcagacccaa catcgggttg gtattgggaa gttgttgcta tatcatctaa tctacaagga agactgacat ctccatcatt gggaagcagg aaaatatttt cacccagtga tgtcgatata ccttttgata acaatttttt tcgctcttat cggataaagg ggatcaacga tcagaagcta caggtgattg agagtgatgt tgtgcttgat aaccagaatc atgttgcgcg ttttcgtctc gttggcaata ttgatgaagc acatgcacaa gtgcaggagt ttaagcggac tttgcaaatt tttctttgga gttttggtat aggcagtgtt cttatcaacc ttgttattat tctctttagt tttcaaccgt tgaagcttat ccgacaagca ttgaatgata ttcgcgaagg aaaagttcat tacatgagta cggatttagt aagcgaagtg atgccgctag cacaagagat gaataccctt attcataatg atcagcgtat tatcgaacgg tttcggaccc aggttggtaa tcttgctcat tcattaaaaa cgccgttgtc agttattatg aatgaggctg ataagatgtg cggagaacag gcgattttat tgagagagca aactaaaata atgcaggacc aaataaatca ttatctaaaa cgcgctcgca ttgcagcaca acgcgacagt gtcgtttatc atgcatctgt tcgcaaagta gttgaccgtt tggtaagggt tatgaaaaag cttaatcctg agaaacagtt tcaatttgtc atggatgtcg atgatattat tttttctggt gaaagggaag atttagaaga agtcgtgggt aatttgattg agaatgctgc tcagtggtct cgcaaaaaag ttctgatttc ttgttattta gaaaaaaata ttgaagaagc agaattcttc agtatcctcg tagaagatga tggttttggt ttaacagaag aacaaattga tgaagctttt aaaagaggtc atcgatttga tgaaagtaag ccgggaacag gtttagggtt agcgattgtt tccgatatgg tcaacgaata tggcggaagc cttttcttat ctcgttctga tttgggcgga ttatatgtga gagttttatt accaaagaag gtaactcttt tatga
Details for BaquA.00863.c.B3.GE39451
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ PLKLIRQALN DIREGKVHYM STDLVSEVMP LAQEMNTLIH NDQRIIERFR TQVGNLAHSL KTPLSVIMNE ADKMCGEQAI LLREQTKIMQ DQINHYLKRA RIAAQRDSVV YHASVRKVVD RLVRVMKKLN PEKQFQFVMD VDDIIFSGER EDLEEVVGNL IENAAQWSRK KVLISCYLEK NIEEAEFFSI LVEDDGFGLT EEQIDEAFKR GHRFDESKPG TGLGLAIVSD MVNEYGGSLF LSRSDLGGLY VRVLLPKKVT LL
Validated NT Sequence
atcttactca cttataaaag agttaccttc tttggtaata aaactctcac atataatccg cccaaatcag aacgagataa gaaaaggctt ccgccatatt cgttgaccat atcggaaaca atcgctaacc ctaaacctgt tcccggctta ctttcatcaa atcgatgacc tcttttaaaa gcttcatcaa tttgttcttc tgttaaacca aaaccatcat cttctacgag gatactgaag aattctgctt cttcaatatt tttttctaaa taacaagaaa tcagaacttt tttgcgagac cactgagcag cattctcaat caaattaccc acgacttctt ctaaatcttc cctttcacca gaaaaaataa tatcatcgac atccatgaca aattgaaact gtttctcagg attaagcttt ttcataaccc ttaccaaacg gtcaactact ttgcgaacag atgcatgata aacgacactg tcgcgttgtg ctgcaatgcg agcgcgtttt agataatgat ttatttggtc ctgcattatt ttagtttgct ctctcaataa aatcgcctgc tctccgcaca tcttatcagc ctcattcata ataactgaca acggcgtttt taatgaatga gcaagattac caacctgggt ccgaaaccgt tcgataatac gctgatcatt atgaataagg gtattcatct cttgtgctag cggcatcact tcgcttacta aatccgtact catgtaatga acttttcctt cgcgaatatc attcaatgct tgtcggataa gcttcaacgg ttgcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHQP LKLIRQALND IREGKVHYMS TDLVSEVMPL AQEMNTLIHN DQRIIERFRT QVGNLAHSLK TPLSVIMNEA DKMCGEQAIL LREQTKIMQD QINHYLKRAR IAAQRDSVVY HASVRKVVDR LVRVMKKLNP EKQFQFVMDV DDIIFSGERE DLEEVVGNLI ENAAQWSRKK VLISCYLEKN IEEAEFFSIL VEDDGFGLTE EQIDEAFKRG HRFDESKPGT GLGLAIVSDM VNEYGGSLFL SRSDLGGLYV RVLLPKKVTL L
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaaccgt tgaagcttat ccgacaagca ttgaatgata ttcgcgaagg aaaagttcat tacatgagta cggatttagt aagcgaagtg atgccgctag cacaagagat gaataccctt attcataatg atcagcgtat tatcgaacgg tttcggaccc aggttggtaa tcttgctcat tcattaaaaa cgccgttgtc agttattatg aatgaggctg ataagatgtg cggagaacag gcgattttat tgagagagca aactaaaata atgcaggacc aaataaatca ttatctaaaa cgcgctcgca ttgcagcaca acgcgacagt gtcgtttatc atgcatctgt tcgcaaagta gttgaccgtt tggtaagggt tatgaaaaag cttaatcctg agaaacagtt tcaatttgtc atggatgtcg atgatattat tttttctggt gaaagggaag atttagaaga agtcgtgggt aatttgattg agaatgctgc tcagtggtct cgcaaaaaag ttctgatttc ttgttattta gaaaaaaata ttgaagaagc agaattcttc agtatcctcg tagaagatga tggttttggt ttaacagaag aacaaattga tgaagctttt aaaagaggtc atcgatttga tgaaagtaag ccgggaacag gtttagggtt agcgattgtt tccgatatgg tcaacgaata tggcggaagc cttttcttat ctcgttctga tttgggcgga ttatatgtga gagttttatt accaaagaag gtaactcttt tatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BaquA.00863.c.A4.PW37826
PURIFICATION DATe: 12/15/2015
CONCENTRATION: 21mg/ml
OBSERVED MW: 29kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 33
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRFRTQVGN LAHSLKTPLS VIMNEADKMC GEQAILLREQ TKIMQDQINH YLKRARIAAQ RDSVVYHASV RKVVDRLVRV MKKLNPEKQF QFVMDVDDII FSGEREDLEE VVGNLIENAA QWSRKKVLIS CYLEKNIEEA EFFSILVEDD GFGLTEEQID EAFKRGHRFD ESKPGTGLGL AIVSDMVNEY GGSLFLSRSD LGGLYVRVLL PK
Validated NT Sequence
tctttggtaa taaaactctc acatataatc cgcccaaatc agaacgagat aagaaaaggc ttccgccata ttcgttgacc atatcggaaa caatcgctaa ccctaaacct gttcccggct tactttcatc aaatcgatga cctcttttaa aagcttcatc aatttgttct tctgttaaac caaaaccatc atcttctacg aggatactga agaattctgc ttcttcaata tttttttcta aataacaaga aatcagaact tttttgcgag accactgagc agcattctca atcaaattac ccacgacttc ttctaaatct tccctttcac cagaaaaaat aatatcatcg acatccatga caaattgaaa ctgtttctca ggattaagct ttttcataac ccttaccaaa cggtcaacta ctttgcgaac agatgcatga taaacgacac tgtcgcgttg tgctgcaatg cgagcgcgtt ttagataatg atttatttgg tcctgcatta ttttagtttg ctctctcaat aaaatcgcct gctctccgca catcttatca gcctcattca taataactga caacggcgtt tttaatgaat gagcaagatt accaacctgg gtccgaaacc gcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SRFRTQVGNL AHSLKTPLSV IMNEADKMCG EQAILLREQT KIMQDQINHY LKRARIAAQR DSVVYHASVR KVVDRLVRVM KKLNPEKQFQ FVMDVDDIIF SGEREDLEEV VGNLIENAAQ WSRKKVLISC YLEKNIEEAE FFSILVEDDG FGLTEEQIDE AFKRGHRFDE SKPGTGLGLA IVSDMVNEYG GSLFLSRSDL GGLYVRVLLP KKV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGCG GTTTCGGACC CAGGTTGGTA ATCTTGCTCA TTCATTAAAA ACGCCGTTGT CAGTTATTAT GAATGAGGCT GATAAGATGT GCGGAGAACA GGCGATTTTA TTGAGAGAGC AAACTAAAAT AATGCAGGAC CAAATAAATC ATTATCTAAA ACGCGCTCGC ATTGCAGCAC AACGCGACAG TGTCGTTTAT CATGCATCTG TTCGCAAAGT AGTTGACCGT TTGGTAAGGG TTATGAAAAA GCTTAATCCT GAGAAACAGT TTCAATTTGT CATGGATGTC GATGATATTA TTTTTTCTGG TGAAAGGGAA GATTTAGAAG AAGTCGTGGG TAATTTGATT GAGAATGCTG CTCAGTGGTC TCGCAAAAAA GTTCTGATTT CTTGTTATTT AGAAAAAAAT ATTGAAGAAG CAGAATTCTT CAGTATCCTC GTAGAAGATG ATGGTTTTGG TTTAACAGAA GAACAAATTG ATGAAGCTTT TAAAAGAGGT CATCGATTTG ATGAAAGTAA GCCGGGAACA GGTTTAGGGT TAGCGATTGT TTCCGATATG GTCAACGAAT ATGGCGGAAG CCTTTTCTTA TCTCGTTCTG ATTTGGGCGG ATTATATGTG AGAGTTTTAT TACCAAAGAA GGTATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA