BaquA.00863.b

Histidine kinase (EC 2.7.13.3)

CENTER ID: BaquA.00863.b
ORGANISM: Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BaquA.00863.b.B3.GE39450 includes HisKA_HATPase_c 185 460
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BaquA.00863.b.A4.PW37825 HisKA HATPase_c 240 460
BaquA.00863.b.B3.PW37837 includes HisKA_HATPase_c 185 460

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283165.4.peg.458
RefSeq: WP_011179187.1
UniProt: A0A0H3M2H6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRLINMMRT TALRLSALYI LLFGLVATGL SIYMTAFSVS LLTEQTEQAL REELRSIENA YNYGGLPLLI RTIDYRSRQP GAFLYLVTDP MGRILAGNVA RIEVGLLKHN GFLSDSFLYS RFGENDKTSE HRALAIIVDL PNAMKILVGR DLDEPERFAA VIRKAVMIAL AAMVGGALLI WFFVGRRALQ RIDRVTAASQ RLMDGDFSGR LPVSGVGDEF DRLSANLNVM LDHIEELNIG LRQVSDNIAH DLKTPLTRLR NRAEAALLGQ KTKFEYRQAL DGIIAESDQL IRTFNAILMI SRIEASSAIE NLETMNMKKI LEDAVEIYEP FAEESGVLLW LGDTFDKELN LNRELIAQSI FNLIDNAIKY ASKDGRKAKV CLSMEYRGEH LLVVVSDNGP GIAADKLEKV TERFVRLEES RTQPGFGIGL SIAKAVMKLH GGELLLENAN PGLKAILLFP
NT Sequence
atgaatcgtt taataaatat gatgcgcaca actgcgctaa ggctttcagc actttatatt ttattgtttg ggttagttgc aacagggctt tctatttata tgacggcgtt ttctgtttca ttgttaactg agcaaactga acaagctttg cgtgaagagc taagaagtat cgaaaacgct tataactatg gtggcctccc tttattaata cgcactattg attaccgttc acgacaacct ggggcttttc tttatcttgt tactgatcct atgggacgca ttttagcagg gaatgttgca cgtattgaag ttggactttt gaagcataat ggttttcttt cggattcttt tttgtattcg cgttttggag aaaatgacaa aacaagtgaa catcgcgctt tggcaattat tgttgatttg cccaatgcta tgaaaattct tgtgggacgg gatttggatg aaccagaacg ttttgcagcg gttattcgta aagcggtaat gattgctctc gcagcaatgg ttggaggggc tttactcata tggttttttg ttggtaggcg cgcgttgcag aggattgatc gtgtaacggc tgcctcacag cgcttgatgg atggtgattt cagtgggcgt ttacctgttt ctggggtagg tgatgagttt gatcgattgt cagctaatct taatgttatg ttagaccaca ttgaagaatt aaatattggt ttgcgtcaag tatcggataa tattgctcat gatttgaaaa caccgctgac gcgtcttaga aatcgtgctg aagcagcact tttgggacaa aaaacgaagt ttgaatatcg tcaagcactt gatggtatca ttgctgaatc ggatcaactt attcgtactt ttaatgctat tctaatgatt tcgcgcattg aggctagcag tgcaattgag aatcttgaga ctatgaatat gaagaagatc cttgaagatg ccgttgaaat ttatgaacct tttgctgaag agtccggtgt tttgctttgg ttgggggata catttgataa agagcttaat ttgaatcgtg agcttattgc acaatcaatt tttaatctta tcgataatgc gattaaatat gcatctaaag atggaaggaa ggcaaaagtt tgcttatcga tggaatatcg tggtgaacat ttgttggttg ttgttagcga caatggccca ggaattgcag cggacaaact tgaaaaagta acagaacgat ttgttcgttt ggaagaaagt cggacacagc ctggttttgg tattggttta agtatagcaa aagctgttat gaaacttcat gggggtgaat tgttacttga aaatgcaaac ccagggctta aggctatttt attatttccg taa
Details for BaquA.00863.b.B3.GE39450
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 32kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG RRALQRIDRV TAASQRLMDG DFSGRLPVSG VGDEFDRLSA NLNVMLDHIE ELNIGLRQVS DNIAHDLKTP LTRLRNRAEA ALLGQKTKFE YRQALDGIIA ESDQLIRTFN AILMISRIEA SSAIENLETM NMKKILEDAV EIYEPFAEES GVLLWLGDTF DKELNLNREL IAQSIFNLID NAIKYASKDG RKAKVCLSME YRGEHLLVVV SDNGPGIAAD KLEKVTERFV RLEESRTQPG FGIGLSIAKA VMKLHGGELL LENANPGLKA ILLFP
Validated NT Sequence
atcttactca cttacggaaa taataaaata gccttaagcc ctgggtttgc attttcaagt aacaattcac ccccatgaag tttcataaca gcttttgcta tacttaaacc aataccaaaa ccaggctgtg tccgactttc ttccaaacga acaaatcgtt ctgttacttt ttcaagtttg tccgctgcaa ttcctgggcc attgtcgcta acaacaacca acaaatgttc accacgatat tccatcgata agcaaacttt tgccttcctt ccatctttag atgcatattt aatcgcatta tcgataagat taaaaattga ttgtgcaata agctcacgat tcaaattaag ctctttatca aatgtatccc ccaaccaaag caaaacaccg gactcttcag caaaaggttc ataaatttca acggcatctt caaggatctt cttcatattc atagtctcaa gattctcaat tgcactgcta gcctcaatgc gcgaaatcat tagaatagca ttaaaagtac gaataagttg atccgattca gcaatgatac catcaagtgc ttgacgatat tcaaacttcg ttttttgtcc caaaagtgct gcttcagcac gatttctaag acgcgtcagc ggtgttttca aatcatgagc aatattatcc gatacttgac gcaaaccaat atttaattct tcaatgtggt ctaacataac attaagatta gctgacaatc gatcaaactc atcacctacc ccagaaacag gtaaacgccc actgaaatca ccatccatca agcgctgtga ggcagccgtt acacgatcaa tcctctgcaa cgcgcgccta cccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHGR RALQRIDRVT AASQRLMDGD FSGRLPVSGV GDEFDRLSAN LNVMLDHIEE LNIGLRQVSD NIAHDLKTPL TRLRNRAEAA LLGQKTKFEY RQALDGIIAE SDQLIRTFNA ILMISRIEAS SAIENLETMN MKKILEDAVE IYEPFAEESG VLLWLGDTFD KELNLNRELI AQSIFNLIDN AIKYASKDGR KAKVCLSMEY RGEHLLVVVS DNGPGIAADK LEKVTERFVR LEESRTQPGF GIGLSIAKAV MKLHGGELLL ENANPGLKAI LLFP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtaggc gcgcgttgca gaggattgat cgtgtaacgg ctgcctcaca gcgcttgatg gatggtgatt tcagtgggcg tttacctgtt tctggggtag gtgatgagtt tgatcgattg tcagctaatc ttaatgttat gttagaccac attgaagaat taaatattgg tttgcgtcaa gtatcggata atattgctca tgatttgaaa acaccgctga cgcgtcttag aaatcgtgct gaagcagcac ttttgggaca aaaaacgaag tttgaatatc gtcaagcact tgatggtatc attgctgaat cggatcaact tattcgtact tttaatgcta ttctaatgat ttcgcgcatt gaggctagca gtgcaattga gaatcttgag actatgaata tgaagaagat ccttgaagat gccgttgaaa tttatgaacc ttttgctgaa gagtccggtg ttttgctttg gttgggggat acatttgata aagagcttaa tttgaatcgt gagcttattg cacaatcaat ttttaatctt atcgataatg cgattaaata tgcatctaaa gatggaagga aggcaaaagt ttgcttatcg atggaatatc gtggtgaaca tttgttggtt gttgttagcg acaatggccc aggaattgca gcggacaaac ttgaaaaagt aacagaacga tttgttcgtt tggaagaaag tcggacacag cctggttttg gtattggttt aagtatagca aaagctgtta tgaaacttca tgggggtgaa ttgttacttg aaaatgcaaa cccagggctt aaggctattt tattatttcc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BaquA.00863.b.A4.PW37825
PURIFICATION DATe: 12/15/2015
CONCENTRATION: 32mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGLRQVSDN IAHDLKTPLT RLRNRAEAAL LGQKTKFEYR QALDGIIAES DQLIRTFNAI LMISRIEASS AIENLETMNM KKILEDAVEI YEPFAEESGV LLWLGDTFDK ELNLNRELIA QSIFNLIDNA IKYASKDGRK AKVCLSMEYR GEHLLVVVSD NGPGIAADKL EKVTERFVRL EESRTQPGFG IGLSIAKAVM KLHGGELLLE NANPGLKAIL LFP
Validated NT Sequence
tattacggaa ataataaaat agccttaagc cctgggtttg cattttcaag taacaattca cccccatgaa gtttcataac agcttttgct atacttaaac caataccaaa accaggctgt gtccgacttt cttccaaacg aacaaatcgt tctgttactt tttcaagttt gtccgctgca attcctgggc cattgtcgct aacaacaacc aacaaatgtt caccacgata ttccatcgat aagcaaactt ttgccttcct tccatcttta gatgcatatt taatcgcatt atcgataaga ttaaaaattg attgtgcaat aagctcacga ttcaaattaa gctctttatc aaatgtatcc cccaaccaaa gcaaaacacc ggactcttca gcaaaaggtt cataaatttc aacggcatct tcaaggatct tcttcatatt catagtctca agattctcaa ttgcactgct agcctcaatg cgcgaaatca ttagaatagc attaaaagta cgaataagtt gatccgattc agcaatgata ccatcaagtg cttgacgata ttcaaacttc gttttttgtc ccaaaagtgc tgcttcagca cgatttctaa gacgcgtcag cggtgttttc aaatcatgag caatattatc cgatacttga cgcaaaccca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SGLRQVSDNI AHDLKTPLTR LRNRAEAALL GQKTKFEYRQ ALDGIIAESD QLIRTFNAIL MISRIEASSA IENLETMNMK KILEDAVEIY EPFAEESGVL LWLGDTFDKE LNLNRELIAQ SIFNLIDNAI KYASKDGRKA KVCLSMEYRG EHLLVVVSDN GPGIAADKLE KVTERFVRLE ESRTQPGFGI GLSIAKAVMK LHGGELLLEN ANPGLKAILL FP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGG TTTGCGTCAA GTATCGGATA ATATTGCTCA TGATTTGAAA ACACCGCTGA CGCGTCTTAG AAATCGTGCT GAAGCAGCAC TTTTGGGACA AAAAACGAAG TTTGAATATC GTCAAGCACT TGATGGTATC ATTGCTGAAT CGGATCAACT TATTCGTACT TTTAATGCTA TTCTAATGAT TTCGCGCATT GAGGCTAGCA GTGCAATTGA GAATCTTGAG ACTATGAATA TGAAGAAGAT CCTTGAAGAT GCCGTTGAAA TTTATGAACC TTTTGCTGAA GAGTCCGGTG TTTTGCTTTG GTTGGGGGAT ACATTTGATA AAGAGCTTAA TTTGAATCGT GAGCTTATTG CACAATCAAT TTTTAATCTT ATCGATAATG CGATTAAATA TGCATCTAAA GATGGAAGGA AGGCAAAAGT TTGCTTATCG ATGGAATATC GTGGTGAACA TTTGTTGGTT GTTGTTAGCG ACAATGGCCC AGGAATTGCA GCGGACAAAC TTGAAAAAGT AACAGAACGA TTTGTTCGTT TGGAAGAAAG TCGGACACAG CCTGGTTTTG GTATTGGTTT AAGTATAGCA AAAGCTGTTA TGAAACTTCA TGGGGGTGAA TTGTTACTTG AAAATGCAAA CCCAGGGCTT AAGGCTATTT TATTATTTCC GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for BaquA.00863.b.B3.PW37837
PURIFICATION DATe: 1/12/2016
CONCENTRATION: 27mg/ml
OBSERVED MW: 32kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG RRALQRIDRV TAASQRLMDG DFSGRLPVSG VGDEFDRLSA NLNVMLDHIE ELNIGLRQVS DNIAHDLKTP LTRLRNRAEA ALLGQKTKFE YRQALDGIIA ESDQLIRTFN AILMISRIEA SSAIENLETM NMKKILEDAV EIYEPFAEES GVLLWLGDTF DKELNLNREL IAQSIFNLID NAIKYASKDG RKAKVCLSME YRGEHLLVVV SDNGPGIAAD KLEKVTERFV RLEESRTQPG FGIGLSIAKA VMKLHGGELL LENANPGLKA ILLFP
Validated NT Sequence
atcttactca cttacggaaa taataaaata gccttaagcc ctgggtttgc attttcaagt aacaattcac ccccatgaag tttcataaca gcttttgcta tacttaaacc aataccaaaa ccaggctgtg tccgactttc ttccaaacga acaaatcgtt ctgttacttt ttcaagtttg tccgctgcaa ttcctgggcc attgtcgcta acaacaacca acaaatgttc accacgatat tccatcgata agcaaacttt tgccttcctt ccatctttag atgcatattt aatcgcatta tcgataagat taaaaattga ttgtgcaata agctcacgat tcaaattaag ctctttatca aatgtatccc ccaaccaaag caaaacaccg gactcttcag caaaaggttc ataaatttca acggcatctt caaggatctt cttcatattc atagtctcaa gattctcaat tgcactgcta gcctcaatgc gcgaaatcat tagaatagca ttaaaagtac gaataagttg atccgattca gcaatgatac catcaagtgc ttgacgatat tcaaacttcg ttttttgtcc caaaagtgct gcttcagcac gatttctaag acgcgtcagc ggtgttttca aatcatgagc aatattatcc gatacttgac gcaaaccaat atttaattct tcaatgtggt ctaacataac attaagatta gctgacaatc gatcaaactc atcacctacc ccagaaacag gtaaacgccc actgaaatca ccatccatca agcgctgtga ggcagccgtt acacgatcaa tcctctgcaa cgcgcgccta cccatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHGR RALQRIDRVT AASQRLMDGD FSGRLPVSGV GDEFDRLSAN LNVMLDHIEE LNIGLRQVSD NIAHDLKTPL TRLRNRAEAA LLGQKTKFEY RQALDGIIAE SDQLIRTFNA ILMISRIEAS SAIENLETMN MKKILEDAVE IYEPFAEESG VLLWLGDTFD KELNLNRELI AQSIFNLIDN AIKYASKDGR KAKVCLSMEY RGEHLLVVVS DNGPGIAADK LEKVTERFVR LEESRTQPGF GIGLSIAKAV MKLHGGELLL ENANPGLKAI LLFP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGTAGGC GCGCGTTGCA GAGGATTGAT CGTGTAACGG CTGCCTCACA GCGCTTGATG GATGGTGATT TCAGTGGGCG TTTACCTGTT TCTGGGGTAG GTGATGAGTT TGATCGATTG TCAGCTAATC TTAATGTTAT GTTAGACCAC ATTGAAGAAT TAAATATTGG TTTGCGTCAA GTATCGGATA ATATTGCTCA TGATTTGAAA ACACCGCTGA CGCGTCTTAG AAATCGTGCT GAAGCAGCAC TTTTGGGACA AAAAACGAAG TTTGAATATC GTCAAGCACT TGATGGTATC ATTGCTGAAT CGGATCAACT TATTCGTACT TTTAATGCTA TTCTAATGAT TTCGCGCATT GAGGCTAGCA GTGCAATTGA GAATCTTGAG ACTATGAATA TGAAGAAGAT CCTTGAAGAT GCCGTTGAAA TTTATGAACC TTTTGCTGAA GAGTCCGGTG TTTTGCTTTG GTTGGGGGAT ACATTTGATA AAGAGCTTAA TTTGAATCGT GAGCTTATTG CACAATCAAT TTTTAATCTT ATCGATAATG CGATTAAATA TGCATCTAAA GATGGAAGGA AGGCAAAAGT TTGCTTATCG ATGGAATATC GTGGTGAACA TTTGTTGGTT GTTGTTAGCG ACAATGGCCC AGGAATTGCA GCGGACAAAC TTGAAAAAGT AACAGAACGA TTTGTTCGTT TGGAAGAAAG TCGGACACAG CCTGGTTTTG GTATTGGTTT AAGTATAGCA AAAGCTGTTA TGAAACTTCA TGGGGGTGAA TTGTTACTTG AAAATGCAAA CCCAGGGCTT AAGGCTATTT TATTATTTCC GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT