SSGCID: Target Page

BaquA.00107.a

Putative uncharacterized protein

CENTER ID:	BaquA.00107.a
ORGANISM:	Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.00107.a.B1.GE37516	Full length(BaquA.00107.a)		1	371
BaquA.00107.a.B2.GE37517	domain based on Ec 2F1M alignment		62	352

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.00107.a.B2.PS02020	domain based on Ec 2F1M alignment		62	352

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283165.4.peg.149
RefSeq:	YP_031845.1
UniProt:	A0A0H3M201

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGLSKKVFPL VFFIAILVVT YWAGKQSSEQ FIPLADKTKN ASNKISKPST ERLPTNVVID LVKVQDFHEQ LNVLGSGQAF AAVELIPWSS GVVDKFFVSA GTKVQEGDVI AKLDSKKEEI AAAKAKMQHD NSALTLSRIL KLRASNTATE VQEIAARLEL DNADLVLRNA ELDLDRRTIR APISGVVGIL PIGEGTAVTL NTVISRIENR DRILVDIWVP ERYVSRIHKG DEVTATLTAQ PNKTFVGHIY AIDNVVDSKT RTLHVQVEIK NEKDTLMSGM SFSVALQFSG DSFPIINPLA VQWNSRGSFV WRVREGKVEP VPVSIIQHKA DQVVVKAPLE NGDQVVIQGV QMLHPGSRVT FDDPKSINSN D

NT Sequence

ATGGGGTTGT CAAAAAAGGT CTTTCCACTG GTGTTTTTCA TTGCCATTTT GGTGGTTACT TATTGGGCAG GCAAACAGAG TTCAGAACAA TTCATTCCAT TAGCGGATAA AACAAAAAAT GCATCGAATA AAATTTCAAA GCCGTCAACG GAAAGGCTGC CAACCAATGT TGTCATTGAT CTTGTAAAAG TTCAAGATTT TCATGAACAG CTAAATGTAC TTGGGAGTGG TCAAGCATTT GCTGCGGTGG AGTTGATCCC TTGGTCATCA GGTGTTGTTG ATAAGTTTTT TGTATCAGCT GGTACAAAAG TACAGGAGGG TGATGTGATT GCAAAGCTTG ATTCTAAAAA GGAAGAAATA GCGGCTGCAA AAGCAAAAAT GCAACATGAT AATAGTGCAT TAACTCTTTC GCGTATTCTT AAATTACGTG CTAGCAATAC AGCCACAGAG GTTCAAGAAA TTGCTGCGCG TTTAGAATTG GATAACGCAG ATTTGGTTTT ACGTAATGCT GAGTTAGACC TCGATCGGCG AACAATTCGT GCACCGATTA GCGGAGTTGT TGGTATTCTA CCCATTGGTG AGGGCACTGC TGTGACCCTT AATACGGTAA TAAGTCGTAT TGAGAATAGA GATCGTATTT TGGTCGATAT ATGGGTTCCT GAACGATATG TGTCACGAAT ACATAAAGGA GATGAAGTGA CTGCAACACT AACGGCGCAG CCGAATAAGA CTTTTGTTGG CCATATTTAT GCGATTGATA ATGTGGTTGA TTCAAAAACC CGCACACTTC ACGTTCAAGT TGAAATTAAG AATGAAAAAG ATACTCTTAT GTCTGGTATG TCTTTTTCTG TTGCGTTGCA ATTTTCTGGT GATTCTTTCC CTATCATTAA TCCTCTTGCA GTTCAATGGA ACAGTAGAGG ATCGTTTGTT TGGCGTGTAA GAGAGGGTAA GGTAGAGCCT GTTCCAGTAT CAATTATTCA GCATAAAGCA GATCAGGTAG TTGTGAAAGC ACCTCTAGAA AATGGTGATC AAGTTGTCAT ACAGGGAGTA CAGATGCTTC ATCCAGGAAG TAGGGTTACT TTTGATGATC CAAAATCCAT CAATAGCAAT GAT

Details for BaquA.00107.a.B1.GE37516

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	62

Validated AA Sequence

MQHDNSALTL SRILKLRASN TATEVQEIAA RLELDNADLV LRNAELDLDR RTIRAPISGV VGILPIGEGT AVTLNTVISR IENRDRILVD IWVPERYVSR IHKGDEVTAT LTAQPNKTFV GHIYAIDNVV DSKTRTLHVQ VEIKNEKDTL MSGMSFSVAL QFSGDSFPII NPLAVQWNSR GSFVWRVREG KVEPVPVSII QHKADQVVVK APLENGDQVV IQGVQMLHPG SRVTF

Validated NT Sequence

caaaagtaac cctacttcct ggatgaagca tctgtactcc ctgtatgaca acttgatcac cattttctag aggtgctttc acaactacct gatctgcttt atgctgaata attgatactg gaacaggctc taccttaccc tctcttacac gccaaacaaa cgatcctcta ctgttccatt gaactgcaag aggattaatg atagggaaag aatcaccaga aaattgcaac gcaacagaaa aagacatacc agacataaga gtatcttttt cattcttaat ttcaacttga acgtgaagtg tgcgggtttt tgaatcaacc acattatcaa tcgcataaat atggccaaca aaagtcttat tcggctgcgc cgttagtgtt gcagtcactt catctccttt atgtattcgt gacacatatc gttcaggaac ccatatatcg accaaaatac gatctctatt ctcaatacga cttattaccg tattaagggt cacagcagtg ccctcaccaa tgggtagaat accaacaact ccgctaatcg gtgcacgaat tgttcgccga tcgaggtcta actcagcatt acgtaaaacc aaatctgcgt tatccaattc taaacgcgca gcaatttctt gaacctctgt ggctgtattg ctagcacgta atttaagaat acgcgaaaga gttaatgcac tattatcatg ttgcattttt gcttttgcag ccgctatttc ttccttttta gaatcaagct ttgcaatcac atcaccctcc tgtacttttg taccagctga tacaaaaaac ttatcaacaa cacctgatga ccaagggatc aactccaccg cagcaaatgc ttgaccactc ccaagtacat ttagctgttc atgaaaatct tgaactttta caagatcaat gacaacattg gttggcagcc tttccgttga cggctttgaa attttattcg atgcattttt tgttttatcc gctaatggaa tgaattgttc tgaactctgt ttgcctgccc aataagtaac caccaaaatg gcaatgaaaa acaccagtgg aaagaccttt tttgacaacc c

Expected Protein Sequence

MAHHHHHHMG LSKKVFPLVF FIAILVVTYW AGKQSSEQFI PLADKTKNAS NKISKPSTER LPTNVVIDLV KVQDFHEQLN VLGSGQAFAA VELIPWSSGV VDKFFVSAGT KVQEGDVIAK LDSKKEEIAA AKAKMQHDNS ALTLSRILKL RASNTATEVQ EIAARLELDN ADLVLRNAEL DLDRRTIRAP ISGVVGILPI GEGTAVTLNT VISRIENRDR ILVDIWVPER YVSRIHKGDE VTATLTAQPN KTFVGHIYAI DNVVDSKTRT LHVQVEIKNE KDTLMSGMSF SVALQFSGDS FPIINPLAVQ WNSRGSFVWR VREGKVEPVP VSIIQHKADQ VVVKAPLENG DQVVIQGVQM LHPGSRVTFD DPKSINSND

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggt tgtcaaaaaa ggtctttcca ctggtgtttt tcattgccat tttggtggtt acttattggg caggcaaaca gagttcagaa caattcattc cattagcgga taaaacaaaa aatgcatcga ataaaatttc aaagccgtca acggaaaggc tgccaaccaa tgttgtcatt gatcttgtaa aagttcaaga ttttcatgaa cagctaaatg tacttgggag tggtcaagca tttgctgcgg tggagttgat cccttggtca tcaggtgttg ttgataagtt ttttgtatca gctggtacaa aagtacagga gggtgatgtg attgcaaagc ttgattctaa aaaggaagaa atagcggctg caaaagcaaa aatgcaacat gataatagtg cattaactct ttcgcgtatt cttaaattac gtgctagcaa tacagccaca gaggttcaag aaattgctgc gcgtttagaa ttggataacg cagatttggt tttacgtaat gctgagttag acctcgatcg gcgaacaatt cgtgcaccga ttagcggagt tgttggtatt ctacccattg gtgagggcac tgctgtgacc cttaatacgg taataagtcg tattgagaat agagatcgta ttttggtcga tatatgggtt cctgaacgat atgtgtcacg aatacataaa ggagatgaag tgactgcaac actaacggcg cagccgaata agacttttgt tggccatatt tatgcgattg ataatgtggt tgattcaaaa acccgcacac ttcacgttca agttgaaatt aagaatgaaa aagatactct tatgtctggt atgtcttttt ctgttgcgtt gcaattttct ggtgattctt tccctatcat taatcctctt gcagttcaat ggaacagtag aggatcgttt gtttggcgtg taagagaggg taaggtagag cctgttccag tatcaattat tcagcataaa gcagatcagg tagttgtgaa agcacctcta gaaaatggtg atcaagttgt catacaggga gtacagatgc ttcatccagg aagtagggtt acttttgatg atccaaaatc catcaatagc aatgattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BaquA.00107.a.B2.GE37517

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV KVQDFHEQLN VLGSGQAFAA VELIPWSSGV VDKFFVSAGT KVQEGDVIAK LDSKKEEIAA AKAKMQHDNS ALTLSRILKL RASNTATEVQ EIAARLELDN ADLVLRNAEL DLDRRTIRAP ISGVVGILPI GEGTAVTLNT VISRIENRDR ILVDIWVPER YVSRIHKGDE VTATLTAQPN KTFVGHIYAI DNVVDSKTRT LHVQVEIKNE KDTLMSGMSF SVALQFSGDS FPIINPLAVQ WNSRGSFVWR VREGKVEPVP VSIIQHKADQ VVVKAPLENG DQVVIQGVQM

Validated NT Sequence

atcttactca cttacatctg tactccctgt atgacaactt gatcaccatt ttctagaggt gctttcacaa ctacctgatc tgctttatgc tgaataattg atactggaac aggctctacc ttaccctctc ttacacgcca aacaaacgat cctctactgt tccattgaac tgcaagagga ttaatgatag ggaaagaatc accagaaaat tgcaacgcaa cagaaaaaga cataccagac ataagagtat ctttttcatt cttaatttca acttgaacgt gaagtgtgcg ggtttttgaa tcaaccacat tatcaatcgc ataaatatgg ccaacaaaag tcttattcgg ctgcgccgtt agtgttgcag tcacttcatc tcctttatgt attcgtgaca catatcgttc aggaacccat atatcgacca aaatacgatc tctattctca atacgactta ttaccgtatt aagggtcaca gcagtgccct caccaatggg tagaatacca acaactccgc taatcggtgc acgaattgtt cgccgatcga ggtctaactc agcattacgt aaaaccaaat ctgcgttatc caattctaaa cgcgcagcaa tttcttgaac ctctgtggct gtattgctag cacgtaattt aagaatacgc gaaagagtta atgcactatt atcatgttgc atttttgctt ttgcagccgc tatttcttcc tttttagaat caagctttgc aatcacatca ccctcctgta cttttgtacc agctgataca aaaaacttat caacaacacc tgatgaccaa gggatcaact ccaccgcagc aaatgcttga ccactcccaa gtacatttag ctgttcatga aaatcttgaa cttttaccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHVK VQDFHEQLNV LGSGQAFAAV ELIPWSSGVV DKFFVSAGTK VQEGDVIAKL DSKKEEIAAA KAKMQHDNSA LTLSRILKLR ASNTATEVQE IAARLELDNA DLVLRNAELD LDRRTIRAPI SGVVGILPIG EGTAVTLNTV ISRIENRDRI LVDIWVPERY VSRIHKGDEV TATLTAQPNK TFVGHIYAID NVVDSKTRTL HVQVEIKNEK DTLMSGMSFS VALQFSGDSF PIINPLAVQW NSRGSFVWRV REGKVEPVPV SIIQHKADQV VVKAPLENGD QVVIQGVQM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtaaaag ttcaagattt tcatgaacag ctaaatgtac ttgggagtgg tcaagcattt gctgcggtgg agttgatccc ttggtcatca ggtgttgttg ataagttttt tgtatcagct ggtacaaaag tacaggaggg tgatgtgatt gcaaagcttg attctaaaaa ggaagaaata gcggctgcaa aagcaaaaat gcaacatgat aatagtgcat taactctttc gcgtattctt aaattacgtg ctagcaatac agccacagag gttcaagaaa ttgctgcgcg tttagaattg gataacgcag atttggtttt acgtaatgct gagttagacc tcgatcggcg aacaattcgt gcaccgatta gcggagttgt tggtattcta cccattggtg agggcactgc tgtgaccctt aatacggtaa taagtcgtat tgagaataga gatcgtattt tggtcgatat atgggttcct gaacgatatg tgtcacgaat acataaagga gatgaagtga ctgcaacact aacggcgcag ccgaataaga cttttgttgg ccatatttat gcgattgata atgtggttga ttcaaaaacc cgcacacttc acgttcaagt tgaaattaag aatgaaaaag atactcttat gtctggtatg tctttttctg ttgcgttgca attttctggt gattctttcc ctatcattaa tcctcttgca gttcaatgga acagtagagg atcgtttgtt tggcgtgtaa gagagggtaa ggtagagcct gttccagtat caattattca gcataaagca gatcaggtag ttgtgaaagc acctctagaa aatggtgatc aagttgtcat acagggagta cagatgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BaquA.00107.a.B2.PS02020

PURIFICATION DATe:	3/19/2014
CONCENTRATION:	10.21mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTAAAAG TTCAAGATTT TCATGAACAG CTAAATGTAC TTGGGAGTGG TCAAGCATTT GCTGCGGTGG AGTTGATCCC TTGGTCATCA GGTGTTGTTG ATAAGTTTTT TGTATCAGCT GGTACAAAAG TACAGGAGGG TGATGTGATT GCAAAGCTTG ATTCTAAAAA GGAAGAAATA GCGGCTGCAA AAGCAAAAAT GCAACATGAT AATAGTGCAT TAACTCTTTC GCGTATTCTT AAATTACGTG CTAGCAATAC AGCCACAGAG GTTCAAGAAA TTGCTGCGCG TTTAGAATTG GATAACGCAG ATTTGGTTTT ACGTAATGCT GAGTTAGACC TCGATCGGCG AACAATTCGT GCACCGATTA GCGGAGTTGT TGGTATTCTA CCCATTGGTG AGGGCACTGC TGTGACCCTT AATACGGTAA TAAGTCGTAT TGAGAATAGA GATCGTATTT TGGTCGATAT ATGGGTTCCT GAACGATATG TGTCACGAAT ACATAAAGGA GATGAAGTGA CTGCAACACT AACGGCGCAG CCGAATAAGA CTTTTGTTGG CCATATTTAT GCGATTGATA ATGTGGTTGA TTCAAAAACC CGCACACTTC ACGTTCAAGT TGAAATTAAG AATGAAAAAG ATACTCTTAT GTCTGGTATG TCTTTTTCTG TTGCGTTGCA ATTTTCTGGT GATTCTTTCC CTATCATTAA TCCTCTTGCA GTTCAATGGA ACAGTAGAGG ATCGTTTGTT TGGCGTGTAA GAGAGGGTAA GGTAGAGCCT GTTCCAGTAT CAATTATTCA GCATAAAGCA GATCAGGTAG TTGTGAAAGC ACCTCTAGAA AATGGTGATC AAGTTGTCAT ACAGGGAGTA CAGATGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T