BamiA.20788.a

BMR1_03g02385; Dipeptidyl peptidase 1 (Cathepsin C) (Cathepsin J) (Dipeptidyl peptidase I) (Dipeptidyl transferase)

CENTER ID: BamiA.20788.a
ORGANISM: Babesia microti RI
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamiA.20788.a.MB21.PW39377 Ben Mamoun construct 21 503

External Resources

RESOURCE REFERENCE ID
EuPathDB: PiroplasmaDB:BMR1_03g02385
UniProt: A0A1R4ABY8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILVLTVSIV RLLSCPFVLG DLPIHALTNE LVGNWKVYLT NTHSEKFLNC GGSSPNNNSS NLHHSLNDYK RFLLDKYGKL TEYDVNFTVE RSVDHSLVFP RNKWKLLNIL DQKHNIIGHW TMVYDVGLNI RMCKIEAFGY LRYTKGNKDE CVDSGNGKNR SKDEFDCYKT DPTKIHIGWA RRDNGTFECF YGEKIGNVEG MGGSTVLFKN MSMDERFKVS GDNVTINSEA SHKYHLFGNQ VYACGRPYPM FKTESLPKNW KWSDLELDSS ETGNQGACGS CYALASIWAI EKRFEIGYKK MMNKTYRIEF DVNSLVRKSP YNQECKGGYP FLVGKDIKDF GIKALNPVFS DRFYVKDYNY VGGCYECTNE TLMMHEIYNF GPIVAAINAG PDFISHKKGI FEGETEIERK RFCDSPDTGL GGWEFTNHAV AIIGWGESEN DGKYWVIKND WGGDWGVNGY AKVRRGINLN GIESQAIYFD PDFESGGGNE ILTKNSRSTV HRT
NT Sequence
atgattcttg ttttaactgt gtctatcgtt aggctactgt catgtccatt tgttttgggt gatctgccta tccatgcctt gacgaatgag ttagttggta actggaaagt atatttgact aatacacatt ctgagaagtt cttgaattgt ggaggatcca gtcctaacaa caattctagc aatttgcatc atagtttaaa tgattacaaa aggtttttgt tagataaata tggtaaattg actgaatatg acgtcaattt cacggttgaa cgaagtgtcg atcattcatt ggtatttcct agaaataaat ggaaattatt gaacatttta gatcaaaaac ataatattat aggccattgg accatggtct atgacgttgg attgaatatt agaatgtgta agatagaagc atttggatat ttaagatata ctaaagggaa caaagatgaa tgtgtagata gtggaaatgg taaaaatcga tctaaagatg aatttgattg ttataaaact gatccaacca agatacacat aggctgggca cgccgtgata atgggacatt tgaatgtttt tatggcgaaa aaatcggaaa tgtcgaagga atgggaggat ctaccgtgtt atttaagaat atgagtatgg atgagagatt caaagtttct ggagataatg tgactataaa cagtgaggcg tcacataagt atcatctttt cggcaaccaa gtatatgctt gcgggcgtcc atatcccatg tttaaaacag aatcattgcc aaaaaattgg aaatggtctg atctagaatt agattcatct gaaactggaa accaaggagc atgtggaagt tgttacgcat tggcttccat ctgggcaatt gaaaagagat ttgaaattgg gtataaaaaa atgatgaata aaacgtatag gatcgagttt gatgttaata gtttggtcag aaaatcgcca tacaatcaag aatgtaaagg tggatatcca tttttagtag gaaaggatat taaagatttt ggcattaaag cattaaaccc tgttttttca gatagattct atgtcaaaga ttacaattac gtgggtggat gttatgaatg taccaatgaa actcttatga tgcatgaaat atacaatttt ggtccaatcg tagctgctat taatgccgga cctgatttta tttctcataa aaaaggaatc tttgaaggag agactgaaat cgagcgtaaa cgtttttgcg attccccaga tacaggttta ggtggctggg aattcacaaa tcatgccgta gctattatag gttggggaga atcggaaaat gatggcaaat attgggtaat taaaaatgat tggggtggag attggggtgt aaatgggtat gcgaaggtaa gaaggggcat aaatttgaat ggtattgagt cgcaagccat ttattttgat cctgattttg aaagtggagg aggcaatgaa attctaacta aaaatagccg aagtacggtg catcgtacat ga
Details for BamiA.20788.a.MB21.PW39377
PURIFICATION DATe: 1/22/2025
CONCENTRATION: 11.3mg/ml
OBSERVED MW: 100kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS DLPIHALTNE LVGNWKVYLT NTHSEKFLNC GGSSPNNNAA NLHHSLNDYK RFLLDKYGKL TEYDVNFAVE RSVDHSLVFP RNKWKLLNIL DQKHNIIGHW TMVYDVGLNI RMCKIEAFGY LRYTKGNKDE CVDSGNGKNR AKDEFDCYKT DPTKIHIGWA RRDNGAFECF YGEKIGNVEG MGGSTVLFKN MSMDERFKVS GDNVAINSEA SHKYHLFGNQ VYACGRPYPM FKTESLPKNW KWSDLELDSS ETGNQGACGS CYALASIWAI EKRFEIGYKK MMNKAYRIEF DVNSLVRKSP YNQECKGGYP FLVGKDIKDF GIKALNPVFS DRFYVKDYNY VGGCYECTNE ALMMHEIYNF GPIVAAINAG PDFISHKKGI FEGETEIERK RFCDSPDTGL GGWEFTNHAV AIIGWGESEN DGKYWVIKND WGGDWGVNGY AKVRRGINLN GIESQAIYFD PDFESGGGNE ILTKNSRSTV HRT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCACA TGAAAATCGA AGAAGGTAAA CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT AATTCGAGCT CGAACAACAA CAACAACAAC AATAACAACA ACCTCGGGAT CGAGGGAAGG ATTTCAGAAG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGGACCT GCCTATCCAC GCCCTGACCA ACGAGCTGGT GGGCAATTGG AAGGTGTACC TGACCAATAC CCACTCTGAA AAGTTCCTGA ACTGCGGCGG AAGCTCTCCA AACAACAACG CCGCCAACCT GCACCACAGC CTGAACGACT ACAAGAGATT TCTGCTGGAT AAGTATGGCA AGCTGACAGA GTACGACGTG AACTTCGCCG TGGAACGGAG CGTGGACCAC AGCCTGGTGT TCCCCAGAAA CAAGTGGAAG CTGCTGAATA TCCTGGACCA GAAGCACAAC ATCATCGGCC ACTGGACCAT GGTGTACGAC GTGGGCCTGA ACATCAGAAT GTGCAAGATC GAGGCTTTCG GCTACCTGAG ATACACCAAG GGCAACAAGG ACGAGTGCGT GGATAGCGGC AACGGCAAGA ATAGAGCCAA GGACGAGTTC GACTGCTACA AGACAGACCC TACAAAGATC CACATCGGCT GGGCCAGAAG AGATAACGGA GCCTTCGAAT GCTTCTACGG CGAGAAAATT GGAAATGTGG AAGGCATGGG CGGTTCTACC GTGCTGTTCA AGAACATGAG CATGGACGAG CGGTTCAAGG TGTCCGGCGA CAACGTGGCT ATCAACAGCG AGGCCTCCCA TAAGTACCAC CTGTTTGGCA ATCAAGTGTA CGCATGCGGC AGACCTTACC CTATGTTCAA GACCGAGAGC CTTCCTAAGA ACTGGAAATG GTCCGATCTG GAACTGGACT CTAGCGAAAC CGGCAACCAG GGCGCCTGCG GATCTTGTTA CGCCCTGGCC AGCATCTGGG CTATCGAAAA GCGGTTTGAG ATCGGCTATA AGAAAATGAT GAACAAAGCC TACAGAATCG AGTTTGACGT GAATAGCCTG GTCAGAAAGA GCCCCTACAA CCAGGAGTGT AAAGGCGGAT ACCCCTTCCT GGTGGGCAAA GATATCAAGG ATTTTGGAAT CAAGGCCCTG AATCCTGTGT TCAGCGATAG ATTTTACGTG AAGGACTACA ACTACGTTGG CGGTTGTTAC GAGTGCACCA ACGAGGCCCT GATGATGCAC GAGATCTACA ACTTCGGACC TATCGTGGCC GCTATCAACG CCGGCCCTGA TTTCATCAGC CACAAGAAGG GCATCTTCGA GGGCGAAACA GAAATCGAGA GAAAGCGGTT CTGCGACAGC CCTGACACCG GCTTGGGCGG ATGGGAGTTC ACAAACCACG CCGTGGCCAT TATCGGATGG GGCGAGTCTG AAAACGACGG CAAGTACTGG GTGATCAAAA ATGACTGGGG AGGAGATTGG GGCGTGAACG GCTACGCTAA GGTGCGGAGA GGCATCAACC TGAATGGCAT CGAGAGCCAG GCCATCTACT TCGACCCCGA CTTTGAAAGC GGCGGGGGAA ATGAGATCCT GACCAAGAAC AGTAGAAGCA CAGTGCACAG AACCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA