SSGCID: Target Page

BamaA.20378.a

Trifunctional purine biosynthetic protein adenosine-3 (GART) (Glycinamide Ribonucleotide Formyltransferase) (GARTFase)

CENTER ID:	BamaA.20378.a
ORGANISM:	Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BamaA.20378.a.B3.GE43488	AIRS domain		499	847

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MESGAAAATR RLRFVVVGSG GREHAIVWKL AQSPRVEKIW VLPGNGGTSE GFFSLPKTAA GHSEAPPPAS VVENVSVPAS NESIVAFAKE HAVDVVVVGP EAPLAEGLAD ALKEGGVACF GPTQAAAQIE ASKAFAKDFM RKYNIPTAEF ATFDNEAEAL AYIDSVGHGV VVKASGLAAG KGVIIPQNKE EAKLAVKQIL TDKCFGDAGQ QVVIEELLQG PEFSLLAFCD GETAIVMPPA QDHKRAYDGD QGPNTGGMGA FAPTPFVSAG LKEQVEETVI KAAVKGMKAE GIPFVGVLFA GLMLTKSGPK VLEYNCRFGD PETQVVLPLL CTDLASIIMA CVTSSLDKTF GPNGQEKVEW LSNQTAVTVV AASGGYPGKY STGKPITGIP NSSSSASSAV VFHAGTKLME DKLVTSGGRV LSVTAVANTL EEALDDAYRG IAAIHFEGMF YRADIGAKAL EAAATAPTRI GILGSTRGTD MQAIIDAIEQ GKLNAEVKMV VSNVEDAYIL ERARLHNLPH TFLPSKGKKR ETFDKQVVQI LDEAGVELVL LIGFMRILSP VFVRHYPRGA IINVHPSLLP DFAGGMDLNV HEEVIKAAVP ETGCTVHVVT EEVDEGPILV QKRCSVDAAD TPETLKAKVQ QLEGQALIEA IQLFQQTVKQ QSKKRQKERQ TRLVEQRQRL ASSKQTTYRD AGVDIDAGDA LVEAIKPFCK ATAREGTTAS LGGFGGLFDL AKTGYKDPIL VSGTDGVGTK IKLAQQCNIH GTVGIDLVAM CVNDLLVQGA DPLFFLDYYA TSKLSVPVAA EVIKGIAEGC LRAGCALIGG ETAEMPGMYH PDEYDLAGFA VGAVEREQLL PRMEDIREGD VVIGVTSSGI HSNGFSMVRH ILQKNSVDVF AKPPFKSDAE RLCDVLLAPT KIYIKSVLPL MNSNLIKAAA HITGGGLPDN IPRVLSDQLA VQLDMSSWEV PPVFRWLQRL GQVSIDEMLR TFNMGIGMVL IVDAERKEEV LSRLRQNEKQ PEEAVVLGSV VPREKEPSPQ VLFVNKSE

NT Sequence

atggagagcg gagctgctgc tgctactcga cgccttaggt ttgtggtggt gggctccgga ggcagggagc acgccatagt gtggaagctc gctcagagcc cacgggtgga gaagatctgg gtccttcccg gaaacggtgg cacctccgag ggcttcttct cgctgccaaa aacagcagca ggccacagcg aggcgcctcc ccctgcctcc gtggtggaga acgtcagtgt gccagcctcc aacgaaagca tcgtggcctt tgccaaggag cacgctgtgg acgtggttgt ggtcggccct gaggctcctc tcgctgaagg cttagctgat gctttgaaag aaggaggagt ggcatgcttc ggaccaactc aagccgctgc tcagatagag gccagcaagg ccttcgccaa ggactttatg cgcaagtaca acatcccaac ggctgagttt gccacttttg acaacgaagc ggaggcctta gcctacatcg actccgttgg gcatggtgtc gtggtgaagg cctctggtct ggctgccggc aagggcgtca tcatccccca gaacaaggaa gaagctaagt tggccgtcaa gcagatactc actgataagt gctttggcga tgctggccaa caggttgtca ttgaagagtt gcttcaagga ccagagtttt ccttgttggc cttctgcgat ggagaaactg cgattgtgat gcctccagct caggaccata aacgagctta cgatggagac caaggaccca acacaggagg aatgggtgca tttgctccta ctccattcgt ctctgctggt ctcaaagagc aagttgaaga aacagtgatc aaagccgctg taaagggaat gaaggcagag ggcattccct ttgtgggcgt ccttttcgcg gggcttatgc ttactaaatc tggccctaaa gtacttgagt acaactgtcg ctttggcgat cccgaaacgc aggtggtact tccgctgctc tgcaccgatc tagcttccat cataatggca tgcgttacct cctctctgga caaaaccttt ggaccgaacg gccaagaaaa ggttgaatgg ctttccaatc aaacagctgt cacagtggta gcagcctctg ggggctatcc aggcaaatac tccactggca aaccaattac tggcattccc aattcttctt cttcagcttc gtcagcagtt gtttttcatg caggcacaaa actcatggaa gataagttgg taacaagcgg aggtagagtc ttgtccgtta ccgcagttgc caacacgttg gaagaggcat tggacgacgc ctatcgcggt attgcagcca ttcatttcga aggcatgttc tacagagctg acattggagc caaagccttg gaggcagcag ctacagctcc aacaagaatc ggcattttgg gatctactag aggaacggac atgcaagcca ttatagacgc catcgagcag ggtaagctca acgccgaggt caagatggtc gtcagcaacg tggaggatgc ctacattctc gaacgtgcaa ggcttcacaa cctgccgcat acgttccttc cctccaaagg aaagaagagg gaaacctttg acaaacaagt cgtgcaaata ttggacgaag ctggggtgga gctcgtgctc ttgatagggt tcatgagaat cctctcacct gttttcgttc gacattatcc acgtggagcc attattaatg tccatccttc tctcttgccc gactttgctg gaggaatgga cttgaacgta catgaagaag tgatcaaagc tgctgttcca gagacaggtt gcacggttca cgtagtaacc gaagaagtcg atgaaggccc catcctcgtt caaaaaagat gcagcgttga tgccgcagat acgccagaaa cgctcaaagc aaaagtgcaa caattggaag gacaagcctt aatcgaagcc attcagctgt ttcaacaaac ggtaaaacaa cagagcaaaa aacgacagaa agagagacaa acaagacttg tagagcagcg acagagatta gcttcttcaa aacaaaccac gtacagagat gctggagttg atattgatgc cggagatgct ttggtcgaag ccatcaaacc gttttgtaaa gcaaccgctc gtgaaggcac tactgcttcc ttaggtggat ttggaggtct attcgactta gccaaaactg gttacaaaga cccaatcctc gtgagcggga cagatggcgt cggcaccaag atcaagctcg ctcaacagtg caacatccac ggcactgtgg gtattgacct ggtggcaatg tgcgtgaacg acctgttggt gcagggcgcc gaccccttgt ttttcctcga ctactatgcc acctccaagt tgagtgttcc cgttgctgct gaggtcatca agggcatcgc tgagggatgc cttcgcgcgg gatgcgcctt gatcggtggt gaaacggccg aaatgcccgg catgtaccat cctgatgagt acgacctggc aggttttgcg gtgggagcag tagagagaga gcagctcctg cctaggatgg aagacataag agaaggagac gtggtgatag gagtgacctc ttccggcatt cactccaacg gcttttcgat ggttcgtcac atactgcaaa agaactcggt cgacgtgttt gccaaaccgc catttaagag cgatgctgaa cgtctctgcg atgttttgtt ggctcctacc aaaatttaca tcaagagcgt actgccattg atgaacagca acttgatcaa agcggcagct cacataacag gaggaggtct tccggacaac atcccccgcg tgctctccga ccagctggcc gttcaactgg acatgtcctc atgggaagtg cctcccgtgt ttcgttggtt gcaacgtttg gggcaggtct ccatcgatga gatgttaaga accttcaaca tgggaatcgg aatggtcctc atcgttgatg ccgagaggaa ggaggaggtg ctatccaggt tgcggcagaa cgagaagcag ccggaagagg ccgttgtgct gggaagcgtt gtgcctcgcg aaaaggaacc aagccctcaa gttttgttcg tgaacaaatc cgagtga

Details for BamaA.20378.a.B3.GE43488

HARVESTED ON:	11/19/2019
SEQUENCED ON:	12/3/2019
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV VSNVEDAYIL ERARLHNLPH TFLPSKGKKR ETFDKQVVQI LDEAGVELVL LIGFMRILSP VFVRHYPRGA IINVHPSLLP DFAGGMDLNV HEEVIKAAVP ETGCTVHVVT EEVDEGPILV QKRCSVDAAD TPETLKAKVQ QLEGQALIEA IQLFQQTVKQ QSKKRQKERQ TRLVEQRQRL ASSKQTTYRD AGVDIDAGDA LVEAIKPFCK ATAREGTTAS LGGFGGLFDL AKTGYKDPIL VSGTDGVGTK IKLAQQCNIH GTVGIDLVAM CVNDLLVQGA DPLFFLDYYA TSKLSVPVAA EVIKGIAEGC LRAGCALIGG ETAEMPGMYH PDEYDLAGFA VGAVERE

Validated NT Sequence

tctactgctc ccaccgcaaa acctgccagg tcgtactcat caggatggta catgccgggc atttcggccg tttcaccacc gatcaaggcg catcccgcgc gaaggcatcc ctcagcgatg cccttgatga cctcagcagc aacgggaaca ctcaacttgg aggtggcata gtagtcgagg aaaaacaagg ggtcggcgcc ctgcaccaac aggtcgttca cgcacattgc caccaggtca atacccacag tgccgtggat gttgcactgt tgagcgagct tgatcttggt gccgacgcca tctgtcccgc tcacgaggat tgggtctttg taaccagttt tggctaagtc gaatagacct ccaaatccac ctaaggaagc agtagtgcct tcacgagcgg ttgctttaca aaacggtttg atggcttcga ccaaagcatc tccggcatca atatcaactc cagcatctct gtacgtggtt tgttttgaag aagctaatct ctgtcgctgc tctacaagtc ttgtttgtct ctctttctgt cgttttttgc tctgttgttt taccgtttgt tgaaacagct gaatggcttc gattaaggct tgtccttcca attgttgcac ttttgctttg agcgtttctg gcgtatctgc ggcatcaacg ctgcatcttt tttgaacgag gatggggcct tcatcgactt cttcggttac tacgtgaacc gtgcaacctg tctctggaac agcagctttg atcacttctt catgtacgtt caagtccatt cctccagcaa agtcgggcaa gagagaagga tggacattaa taatggctcc acgtggataa tgtcgaacga aaacaggtga gaggattctc atgaacccta tcaagagcac gagctccacc ccagcttcgt ccaatatttg cacgacttgt ttgtcaaagg tttccctctt ctttcctttg gagggaagga acgtatgcgg caggttgtga agccttgcac gttcgagaat gtaggcatcc tccacgttgc tgacgaccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtcg tcagcaacgt ggaggatgcc tacattctcg aacgtgcaag gcttcacaac ctgccgcata cgttccttcc ctccaaagga aagaagaggg aaacctttga caaacaagtc gtgcaaatat tggacgaagc tggggtggag ctcgtgctct tgatagggtt catgagaatc ctctcacctg ttttcgttcg acattatcca cgtggagcca ttattaatgt ccatccttct ctcttgcccg actttgctgg aggaatggac ttgaacgtac atgaagaagt gatcaaagct gctgttccag agacaggttg cacggttcac gtagtaaccg aagaagtcga tgaaggcccc atcctcgttc aaaaaagatg cagcgttgat gccgcagata cgccagaaac gctcaaagca aaagtgcaac aattggaagg acaagcctta atcgaagcca ttcagctgtt tcaacaaacg gtaaaacaac agagcaaaaa acgacagaaa gagagacaaa caagacttgt agagcagcga cagagattag cttcttcaaa acaaaccacg tacagagatg ctggagttga tattgatgcc ggagatgctt tggtcgaagc catcaaaccg ttttgtaaag caaccgctcg tgaaggcact actgcttcct taggtggatt tggaggtcta ttcgacttag ccaaaactgg ttacaaagac ccaatcctcg tgagcgggac agatggcgtc ggcaccaaga tcaagctcgc tcaacagtgc aacatccacg gcactgtggg tattgacctg gtggcaatgt gcgtgaacga cctgttggtg cagggcgccg accccttgtt tttcctcgac tactatgcca cctccaagtt gagtgttccc gttgctgctg aggtcatcaa gggcatcgct gagggatgcc ttcgcgcggg atgcgccttg atcggtggtg aaacggccga aatgcccggc atgtaccatc ctgatgagta cgacctggca ggttttgcgg tgggagcagt agagagagag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat