SSGCID: Target Page

BamaA.20377.a

Xylose isomerase (xylA)

CENTER ID:	BamaA.20377.a
ORGANISM:	Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BamaA.20377.a.B1.GE43486	full length		1	445

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BamaA.20377.a.B1.PW38790	full length		1	445

External Resources

RESOURCE	REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEGSSSVEHF PEIKKIEYEG PKSKNPLAFR FYNPEQVVMG KTMKEWLRFS VCYWHTWRGG GMDMFGPATM KRSWDDGSNS LETALRIVDV HFEFLEKLGV EYFCFHDRDI APEGKTLEET NKNLDVVVAK IKQKLQESNG KFKVLWGTAN LFSAVRYMNG AATNPDAHVF AYAAAQVKKA LEISKELGAE CYVFWGGREG YFTLLNTDMR KDLDHYARFL QMAVDYKKEI GFTGQFLIEP KPREPTKHQY DYDCAAVMAF LKQYGLEREF KLNIEANHCT LAGHTFEHEL QYASAYGLLG SVDANTGDPL LGWDTDQFNT DIKSCTLAME VILRQNGLNP GGLNFDCKLR RESTDLEDLF IGHISGMDTF ARGLLAAAAI IEEGTLPALR RERYASWEEE GGIGRKIEDG TTDFKELEAW VLKAGEPKQV SGKQEKYEVL LNTYM

NT Sequence

atggaaggta gcagcagcgt tgaacacttt ccagagatca agaaaattga gtatgaaggc ccaaagtcga agaatccttt ggcctttcgc ttctacaatc ctgagcaggt ggtgatgggc aagaccatga aggagtggct gcgcttttca gtgtgttact ggcacacctg gcgtggcggc ggcatggaca tgtttggccc tgccaccatg aagcgctctt gggatgacgg ttcaaactcg ttggaaacgg cgctcaggat cgtggacgtc cactttgagt tcttggagaa gctcggcgtg gagtactttt gcttccacga ccgcgacatt gctcccgagg gcaagaccct ggaggagacc aacaagaact tggacgtggt ggtggccaag atcaagcaga agctacagga gtccaacggc aagttcaagg tcctttgggg cactgccaac ctgttctctg ctgtcaggta catgaacggt gctgccacta acccggacgc gcatgtcttt gcttatgcgg ctgctcaggt gaagaaagct ctggagatct ccaaagagtt gggtgccgag tgctatgtct tctggggtgg aagggagggt tattttacgc ttctcaacac tgacatgaga aaggatctgg accactacgc ccgattcctc cagatggctg tggattacaa gaaggagatt ggctttactg gccaattcct gattgagccc aagccccgtg aacccaccaa acatcagtat gactatgact gcgctgccgt catggccttc ctcaagcagt acggactaga gagggagttc aagctcaaca ttgaagccaa ccactgcacc ctcgctggtc acacctttga gcacgaactc caatatgcct cggcctatgg cctgcttgga agcgttgacg ccaacacggg tgacccattg ctgggctggg acactgatca gttcaacacc gatatcaaga gctgcacttt ggccatggag gtcatcctcc gccagaacgg cctcaacccg ggcggtctca actttgactg caagctgcgc agggagtcca ccgacctgga ggacctcttc attggccaca taagcggtat ggatacattt gctcgtgggc tgctggcggc tgccgccatc atcgaggaag gcacactccc cgccctccgc cgcgagcgct acgcctcctg ggaggaggaa ggcggcattg ggcgtaagat cgaggacggc accaccgact ttaaggagct ggaggcctgg gtgctgaaag cgggagagcc caagcaagtc tctggcaagc aggagaagta tgaggtcttg ctcaacactt atatgtaa

Details for BamaA.20377.a.B1.GE43486

HARVESTED ON:	11/19/2019
SEQUENCED ON:	12/3/2019
EXPECTED MW:	52kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME GSSSVEHFPE IKKIEYEGPK SKNPLAFRFY NPEQVVMGKT MKEWLRFSVC YWHTWRGGGM DMFGPATMKR SWDDGSNSLE TALRIVDVHF EFLEKLGVEY FCFHDRDIAP EGKTLEETNK NLDVVVAKIK QKLQESNGKF KVLWGTANLF SAVRYMNGAA TNPDAHVFAY AAAQVKKALE ISKELGAECY VFWGGREGYF TLLNTDMRKD LDHYARFLQM AVDYKKEIGF TGQFLIEPKP REPTKHQYDY DCAAVMAFLK QYGLEREFKL NIEANHCTLA GHTFEHELQY ASAYGPLGSV DANTGDPLLG WDTDQFNTDI KSCTLAMEVI LRQNGLNPGG LNFDCKLRRE STDLEDLFIG HISGMDTFAR GLLAAAAIIE EGTLPALRRE RYASWEEEGG IGRKIEDGTT DFKELEAWVL KAGEPKQVSG KQEKYEVLLN TYM

Validated NT Sequence

atacttctcc tgcttgccag agacttgctt gggctctccc gctttcagca cccaggcctc cagctcctta aagtcggtgg tgccgtcctc gatcttacgc ccaatgccgc cttcctcctc ccaggaggcg tagcgctcgc ggcggagggc ggggagtgtg ccttcctcga tgatggcggc agccgccagc agcccacgag caaatgtatc cataccgctt atgtggccaa tgaagaggtc ctccaggtcg gtggactccc tgcgcagctt gcagtcaaag ttgagaccgc ccgggttgag gccgttctgg cggaggatga cctccatggc caaagtgcag ctcttgatat cggtgttgaa ctgatcagtg tcccagccca gcaatgggtc acccgtgttg gcgtcaacgc ttccaagcgg gccataggcc gaggcatatt ggagttcgtg ctcaaaggtg tgaccagcga gggtgcagtg gttggcttca atgttgagct tgaactccct ctctagtccg tactgcttga ggaaggccat gacggcagcg cagtcatagt catactgatg tttggtgggt tcacggggct tgggctcaat caggaattgg ccagtaaagc caatctcctt cttgtaatcc acagccatct ggaggaatcg ggcgtagtgg tccagatcct ttctcatgtc agtgttgaga agcgtaaaat aaccctccct tccaccccag aagacatagc actcggcacc caactctttg gagatctcca gagctttctt cacctgagca gccgcataag caaagacatg cgcgtccggg ttagtggcag caccgttcat gtacctgaca gcagagaaca ggttggcagt gccccaaagg accttgaact tgccgttgga ctcctgtagc ttctgcttga tcttggccac caccacgtcc aagttcttgt tggtctcctc cagggtcttg ccctcgggag caatgtcgcg gtcgtggaag caaaagtact ccacgccgag cttctccaag aactcaaagt ggacgtccac gatcctgagc gccgtttcca acgagtttga accgtcatcc caagagcgct tcatggtggc agggccaaac atgtccatgc cgccgccacg ccaggtgtgc cagtaacaca ctgaaaagcg cagccactcc ttcatggtct tgcccatcac cacctgctca ggattgtaga agcgaaaggc caaaggattc ttcgactttg ggccttcata ctcaattttc ttgatctctg gaaagtgttc aacgctgctg ctaccttcca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHME GSSSVEHFPE IKKIEYEGPK SKNPLAFRFY NPEQVVMGKT MKEWLRFSVC YWHTWRGGGM DMFGPATMKR SWDDGSNSLE TALRIVDVHF EFLEKLGVEY FCFHDRDIAP EGKTLEETNK NLDVVVAKIK QKLQESNGKF KVLWGTANLF SAVRYMNGAA TNPDAHVFAY AAAQVKKALE ISKELGAECY VFWGGREGYF TLLNTDMRKD LDHYARFLQM AVDYKKEIGF TGQFLIEPKP REPTKHQYDY DCAAVMAFLK QYGLEREFKL NIEANHCTLA GHTFEHELQY ASAYGLLGSV DANTGDPLLG WDTDQFNTDI KSCTLAMEVI LRQNGLNPGG LNFDCKLRRE STDLEDLFIG HISGMDTFAR GLLAAAAIIE EGTLPALRRE RYASWEEEGG IGRKIEDGTT DFKELEAWVL KAGEPKQVSG KQEKYEVLLN TYM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag gtagcagcag cgttgaacac tttccagaga tcaagaaaat tgagtatgaa ggcccaaagt cgaagaatcc tttggccttt cgcttctaca atcctgagca ggtggtgatg ggcaagacca tgaaggagtg gctgcgcttt tcagtgtgtt actggcacac ctggcgtggc ggcggcatgg acatgtttgg ccctgccacc atgaagcgct cttgggatga cggttcaaac tcgttggaaa cggcgctcag gatcgtggac gtccactttg agttcttgga gaagctcggc gtggagtact tttgcttcca cgaccgcgac attgctcccg agggcaagac cctggaggag accaacaaga acttggacgt ggtggtggcc aagatcaagc agaagctaca ggagtccaac ggcaagttca aggtcctttg gggcactgcc aacctgttct ctgctgtcag gtacatgaac ggtgctgcca ctaacccgga cgcgcatgtc tttgcttatg cggctgctca ggtgaagaaa gctctggaga tctccaaaga gttgggtgcc gagtgctatg tcttctgggg tggaagggag ggttatttta cgcttctcaa cactgacatg agaaaggatc tggaccacta cgcccgattc ctccagatgg ctgtggatta caagaaggag attggcttta ctggccaatt cctgattgag cccaagcccc gtgaacccac caaacatcag tatgactatg actgcgctgc cgtcatggcc ttcctcaagc agtacggact agagagggag ttcaagctca acattgaagc caaccactgc accctcgctg gtcacacctt tgagcacgaa ctccaatatg cctcggccta tggcctgctt ggaagcgttg acgccaacac gggtgaccca ttgctgggct gggacactga tcagttcaac accgatatca agagctgcac tttggccatg gaggtcatcc tccgccagaa cggcctcaac ccgggcggtc tcaactttga ctgcaagctg cgcagggagt ccaccgacct ggaggacctc ttcattggcc acataagcgg tatggataca tttgctcgtg ggctgctggc ggctgccgcc atcatcgagg aaggcacact ccccgccctc cgccgcgagc gctacgcctc ctgggaggag gaaggcggca ttgggcgtaa gatcgaggac ggcaccaccg actttaagga gctggaggcc tgggtgctga aagcgggaga gcccaagcaa gtctctggca agcaggagaa gtatgaggtc ttgctcaaca cttatatgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for BamaA.20377.a.B1.PW38790

PURIFICATION DATe:	1/22/2020
CONCENTRATION:	60mg/ml
OBSERVED MW:	51kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHME GSSSVEHFPE IKKIEYEGPK SKNPLAFRFY NPEQVVMGKT MKEWLRFSVC YWHTWRGGGM DMFGPATMKR SWDDGSNSLE TALRIVDVHF EFLEKLGVEY FCFHDRDIAP EGKTLEETNK NLDVVVAKIK QKLQESNGKF KVLWGTANLF SAVRYMNGAA TNPDAHVFAY AAAQVKKALE ISKELGAECY VFWGGREGYF TLLNTDMRKD LDHYARFLQM AVDYKKEIGF TGQFLIEPKP REPTKHQYDY DCAAVMAFLK QYGLEREFKL NIEANHCTLA GHTFEHELQY ASAYGLLGSV DANTGDPLLG WDTDQFNTDI KSCTLAMEVI LRQNGLNPGG LNFDCKLRRE STDLEDLFIG HISGMDTFAR GLLAAAAIIE EGTLPALRRE RYASWEEEGG IGRKIEDGTT DFKELEAWVL KAGEPKQVSG KQEKYEVLLN TYM

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAG GTAGCAGCAG CGTTGAACAC TTTCCAGAGA TCAAGAAAAT TGAGTATGAA GGCCCAAAGT CGAAGAATCC TTTGGCCTTT CGCTTCTACA ATCCTGAGCA GGTGGTGATG GGCAAGACCA TGAAGGAGTG GCTGCGCTTT TCAGTGTGTT ACTGGCACAC CTGGCGTGGC GGCGGCATGG ACATGTTTGG CCCTGCCACC ATGAAGCGCT CTTGGGATGA CGGTTCAAAC TCGTTGGAAA CGGCGCTCAG GATCGTGGAC GTCCACTTTG AGTTCTTGGA GAAGCTCGGC GTGGAGTACT TTTGCTTCCA CGACCGCGAC ATTGCTCCCG AGGGCAAGAC CCTGGAGGAG ACCAACAAGA ACTTGGACGT GGTGGTGGCC AAGATCAAGC AGAAGCTACA GGAGTCCAAC GGCAAGTTCA AGGTCCTTTG GGGCACTGCC AACCTGTTCT CTGCTGTCAG GTACATGAAC GGTGCTGCCA CTAACCCGGA CGCGCATGTC TTTGCTTATG CGGCTGCTCA GGTGAAGAAA GCTCTGGAGA TCTCCAAAGA GTTGGGTGCC GAGTGCTATG TCTTCTGGGG TGGAAGGGAG GGTTATTTTA CGCTTCTCAA CACTGACATG AGAAAGGATC TGGACCACTA CGCCCGATTC CTCCAGATGG CTGTGGATTA CAAGAAGGAG ATTGGCTTTA CTGGCCAATT CCTGATTGAG CCCAAGCCCC GTGAACCCAC CAAACATCAG TATGACTATG ACTGCGCTGC CGTCATGGCC TTCCTCAAGC AGTACGGACT AGAGAGGGAG TTCAAGCTCA ACATTGAAGC CAACCACTGC ACCCTCGCTG GTCACACCTT TGAGCACGAA CTCCAATATG CCTCGGCCTA TGGCCTGCTT GGAAGCGTTG ACGCCAACAC GGGTGACCCA TTGCTGGGCT GGGACACTGA TCAGTTCAAC ACCGATATCA AGAGCTGCAC TTTGGCCATG GAGGTCATCC TCCGCCAGAA CGGCCTCAAC CCGGGCGGTC TCAACTTTGA CTGCAAGCTG CGCAGGGAGT CCACCGACCT GGAGGACCTC TTCATTGGCC ACATAAGCGG TATGGATACA TTTGCTCGTG GGCTGCTGGC GGCTGCCGCC ATCATCGAGG AAGGCACACT CCCCGCCCTC CGCCGCGAGC GCTACGCCTC CTGGGAGGAG GAAGGCGGCA TTGGGCGTAA GATCGAGGAC GGCACCACCG ACTTTAAGGA GCTGGAGGCC TGGGTGCTGA AAGCGGGAGA GCCCAAGCAA GTCTCTGGCA AGCAGGAGAA GTATGAGGTC TTGCTCAACA CTTATATGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT