BamaA.20376.a

Exportin-1 (CRM1, XPO1)

CENTER ID: BamaA.20376.a
ORGANISM: Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamaA.20376.a.B1.GE43485 full length 1 1068
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MERILNFDEP LDVKLLDQVV YTFYTGSGPQ QAEAERVLAQ LQEHPQAWSR VDTILEYSES QSTKFLALSI LEKVILYRWK SLPRAQCDGI RNYIVNLVIK LSSDAATMQK ERFYLKKLNM ILVQIIKQEW PRNWETFIPE LVGASQKNES LCENNMEILK LLSEEVFDFS GGQLTQDKIK ELKTSFNKEF SLIYQLCEYI LNTSQSPSLI EATLKTLLRF LNWIPVGYIF QTKMVENLIF KFFPIAPFQN ITLQCLTEVV GIKMGEQNEG IFTQLFTGFM QQLHNILPPS TNLAEVYENG TEEQQQFIQN LAIFFGVFFK NHLKVLESEG THMLLLEAHN YLIEISQVTD SEVWKICLEY WTTLASSLYQ EFPAAPSQVL MLNSHPSQSN SPRRSFYAPI MSRLRKVIIQ RMPKPEEVLI VEDENGEIVR EVMKDSDAII LYKSMRETLI YLTHLDYEDT QSIMLEKLNN QVNGQEWSWN NLNTLCWAIG SISGAQSEED EKRFLVTVIK ELLGLCEMKR GKDNKAVIAS NIMYIVGQYP RFLRAHWKFL KTVVNKLFEF MHETHPGVQD MACDTFLKIA QKCRRKFVVC QIGETAPFVE EILTDLPVII SDLEPQQIQT FYEAVGFMIH SHTDPKARKE LVIKLMALPN STWTRIMEEA QRDVSYLKQT QTCKSITTIL KTNVRAAKSL GHDYIYQLGT IFMDMLNVYK VYSTEISNTV ASNGPNATKT SIVRSMRAVK KETLTLQETF IEKSEDPQIV MQNFIPPLLE AVLIDYKNNI PDARDPEVLS LMTVIINKFK QGLNDQIPPI LAAVFECTLQ MITQNFEDYP VCRVNFFNLI RAINTHCFPA FFQIPPETFK LIIDSIVWAF KHTMRNIAET GLNILLELLK NIAASGEEVA QGFYKAYFIN LVQDIFYVLT DAFHKSSFSL QATILARMFH LVESGQVRVP LWDTTTNQDP AMNNSVFLRN HMTHLLLTAF PHLSKETVHQ FVMGLFERNT DISAFKLLLR DFLVQLKEFS EQDNSELFLE EKEQQLAEAQ SEQLKRDMAI PGMVAPHDAR VQDNSQME
NT Sequence
atggaacgca tactcaactt tgatgagcca ctcgatgtga agttactcga tcaagtggtt tataccttct acacgggctc tgggccacag caagcagagg ccgaaagggt acttgcacag ttgcaagagc atccacaagc atggtcgagg gtggacacca tcctcgaata ctctgagagt caaagcacaa agtttctcgc tctctccatt ctggagaagg tcatcctcta tcgatggaaa agcctgccga gggcgcagtg tgatggcatt cgtaactaca ttgtcaatct tgtgatcaag ctgtcctctg acgctgccac catgcagaag gagcggttct acttgaagaa gttgaatatg atcctcgtgc agattatcaa acaagagtgg ccgcgcaatt gggaaacctt cattcccgaa ctcgttggag ctagtcaaaa gaacgaatcg ttgtgcgaga acaacatgga gatactaaag ctgctcagcg aggaagtgtt tgacttctca ggtggtcaac tgactcaaga caaaataaag gagttgaaga cttcgttcaa caaggagttt tctctcatct accagctttg tgaatacata ctgaacacct cacagagccc ctcccttatt gaggccacct tgaaaacctt gctgcggttt ctcaactgga tccctgtagg ctatattttc caaacaaaga tggttgaaaa tctaatattt aagttctttc caattgcgcc tttccaaaat attactttgc aatgtctgac cgaagttgtt ggcattaaaa tgggagagca gaatgaaggc atttttactc agctgtttac agggtttatg cagcaacttc acaatatatt gccaccttct acgaatctcg ctgaggttta cgagaatggc accgaggagc aacagcagtt tattcagaac cttgccatct tctttggcgt ctttttcaag aatcacctca aggtattgga gtcggaaggc actcacatgc tcctgttgga agctcacaac taccttattg aaatatctca agttaccgat agcgaggtgt ggaagatctg tctcgagtat tggaccacac tggcaagttc actctaccaa gagtttcctg ccgctccttc acaggttctc atgttgaaca gccatccctc tcaatccaac tcgcccagac gctcattcta cgctcctatt atgtctcgac tacgtaaagt tatcattcaa cgcatgccca agcctgagga agtcttgatt gtagaagatg aaaacggaga gatcgttagg gaggtgatga aggatagtga cgccatcatc ttgtacaaga gcatgagaga gacgttgatc tatttgacgc atcttgatta tgaagacact caaagcatca tgctcgaaaa gctgaacaat caggtgaatg gtcaagaatg gtcctggaac aacctcaaca ctctctgctg ggccattgga tccattagcg gagctcaatc tgaagaggac gagaaacgat ttttggttac tgttattaag gagcttttgg gcttgtgcga aatgaaacga ggcaaagata ataaggcagt catcgcaagc aacattatgt atatagttgg gcaatatcct cgcttccttc gcgctcattg gaagttttta aagacggtag ttaacaagct tttcgagttt atgcacgaga ctcaccctgg tgtacaagac atggcttgtg atactttcct caagattgcc caaaagtgta ggcggaaatt cgtggtgtgt caaattggag aaacggcacc ttttgtagag gagattttga ccgatttgcc cgtcattatt tccgacctcg aaccacaaca gatacaaact ttttacgaag cagttggttt tatgattcac tcccacactg accccaaagc acggaaagag ctcgtcatta agttgatggc attacccaat tcaacgtgga cacgaattat ggaagaagca caacgggatg tctcttactt gaagcaaacg caaacatgta agagcatcac cacaatactc aagacaaacg tccgcgcagc taagtcactt ggccatgact acatttacca attgggaact atattcatgg acatgctcaa tgtgtacaaa gtttacagca cggaaatctc caacactgtt gcgtccaatg gtcccaatgc tacaaaaacg tctattgtcc gaagtatgag agcggtaaag aaagaaacat tgactttgca agagaccttt atagagaaat ccgaagatcc tcaaatcgtt atgcaaaact tcattccccc attgcttgag gcggtgctca ttgattacaa gaacaacatt ccagacgcac gcgacccaga agtgttgtct ttaatgactg ttatcataaa caaattcaag caaggattaa acgaccagat acctcctatc ttggctgctg tgtttgagtg cacgctgcaa atgattacac aaaactttga ggactacccc gtctgccgag tgaacttttt caacctcatc cgcgccatca atacccactg cttccctgcc ttcttccaaa ttccaccaga aaccttcaag ctcatcatcg acagcattgt gtgggccttc aagcacacca tgcgaaacat tgctgagacg ggactcaaca tcctgttaga gctgttgaag aacattgcgg cctctgggga ggaggtggcc caaggattct acaaggccta ttttatcaat ctcgtgcagg acatcttcta cgtcttgacc gacgctttcc acaaatcaag tttttctttg caagctacaa tactcgctcg aatgttccac ttggtagaaa gtggtcaagt gcgagtgcct ctgtgggaca caacgaccaa ccaggatcct gccatgaaca actccgtttt cttgcgaaac catatgaccc atctattgct caccgctttc cctcatcttt ccaaagaaac cgtgcatcaa ttcgtgatgg gcctctttga gcggaacaca gacatctccg cgttcaagtt gctgcttcga gactttttgg tgcagctcaa ggaattttcg gagcaggaca attcggagct gttcctagag gagaaggagc agcagttggc cgaggcgcag agcgagcagc tcaagcgaga catggccatt cctggcatgg ttgccccgca cgatgctcga gtgcaagata actcacagat ggaatag
Details for BamaA.20376.a.B1.GE43485
HARVESTED ON: 11/19/2019
SEQUENCED ON: 2/5/2020
EXPECTED MW: 124kDa
OBSERVED MW: 124kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 84
Validated AA Sequence
XAHHHHHHME RILNFDEPLD VKLLDQVVYT FYTGSGPQQA EAERVLAQLQ EHPQAWSRVD TILEYSESQS TKFLALSILE KVILYRWKSL PRAQCDGIRN YIVNLVIKLS SDAATMQKER FYLKKLNMIL VQIIKQEWPR NWETFIPELV GASQKNESLC ENNMEILKLL SEEVFDFSGG QLTQDKIKEL KTSFNKEFSL IYQLCEYILN TSQSPSLIEA TLKTLLRFLN WIPVGYIFQT KMVENLIFKF FPIAPFQNIT LQCLTEVVGI KMGEQNEGIF TQLFTGFMQQ LHNILPPSTN LAEVYENGTE EQQQFIQNLA IFFGVFFKNH LKVLESEGTH MLLLEAHNYL IEISQVTDSE VWKICLEYWT TLASSLYQEF PAAPSQVLML NSHPSQSNSP RRSFYAPIMS RLRKVIIQRM PKPEEVLIVE DENGEIVREV MKDSDAIILY KSMRETLIYL THLDYEDTQS IMLEKLNNQV NGQEWSWNNL NTLCWAIGSI SGAQSEEDEK RFLVTVIKEL LGLCEMKRGK DNKAVIASNI MYIVGQYPRF LRAHWKFLKT VVNKLFEFMH ETHPGXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXSMRAVKKE TLTLQETFIE KSEDPQIVMQ NFIPPLLEAV LIDYKNNIPD ARDPEVLSLM TVIINKFKQG LNDQIPPILA AVFECTLQMI TQNFEDYPVC RVNFFNLIRA INTHCFPAFF QIPPETFKLI IDSIVWAFKH TMRNIAETGL NILLELLKNI AASGEEVAQG FYKAYFINLV QDIFYVLTDA FHKSSFSLQA TILARMFHLV ESGQVRVPLW DTTTNQDPAM NNSVFLRNHM THLLLTAFPH LSKETVHQFV MGLFERNTDI SAFKLLLRDF LVQLKEFSEQ DNSELFLEEK EQQLAEAQSE QLKRDMAIPG MVAPHDARVQ DNSQME
Validated NT Sequence
atggctcacc accaccacca ccatatggaa cgcatactca actttgatga gccactcgat gtgaagttac tcgatcaagt ggtttatacc ttctacacgg gctctgggcc acagcaagca gaggccgaaa gggtacttgc acagttgcaa gagcatccac aagcatggtc gagggtggac accatcctcg aatactctga gagtcaaagc acaaagtttc tcgctctctc cattctggag aaggtcatcc tctatcgatg gaaaagcctg ccgagggcgc agtgtgatgg cattcgtaac tacattgtca atcttgtgat caagctgtcc tctgacgctg ccaccatgca gaaggagcgg ttctacttga agaagttgaa tatgatcctc gtgcagatta tcaaacaaga gtggcctcgc aactgggaaa ccttcattcc cgaactcgtt ggagctagtc aaaagaacga atcgttgtgc gagaacaaca tggagatact aaagctgctc agcgaggaag tgtttgactt ctcaggtggt caactgactc aagacaaaat aaaggagttg aagacttcgt tcaacaagga gttttctctc atctaccagc tttgtgaata catactgaac acctcacaga gcccctccct tattgaggcc accttgaaaa ccttgctgcg gtttctcaac tggatccctg taggctatat tttccaaaca aagatggttg aaaatctaat atttaagttc tttccaattg cgcctttcca aaatattact ttgcaatgtc tgaccgaagt tgttggcatt aaaatgggag agcagaatga aggcattttt actcagctgt ttacagggtt tatgcagcaa cttcacaata tattgccacc ttctacgaat ctcgctgagg tttacgagaa tggcaccgag gagcaacagc agtttattca gaaccttgcc atcttctttg gcgtcttttt caagaatcac ctcaaggtat tggagtcgga aggcactcac atgctcctgt tggaagctca caactacctt attgaaatat ctcaagttac cgatagcgag gtgtggaaga tctgtctcga gtattggacc acactggcaa gttcactcta ccaagagttt cctgccgctc cttcacaggt tctcatgttg aacagccatc cctctcaatc caactcgccc agacgctcat tctacgctcc tattatgtct cgactacgta aagttatcat tcaacgcatg cccaagcctg aggaagtctt gattgtagaa gatgaaaacg gagagatcgt tagggaggtg atgaaggata gtgacgccat catcttgtac aagagcatga gagagacgtt gatctatttg acgcatcttg attatgaaga cactcaaagc atcatgctcg aaaagctgaa caatcaggtg aatggtcaag aatggtcctg gaacaacctc aacactctct gctgggccat tggatccatt agcggagctc aatctgaaga ggacgagaaa cgatttttgg ttactgttat taaggagctt ttgggcttgt gcgaaatgaa acgaggcaaa gataataagg cagtcatcgc aagcaacatt atgtatatag ttgggcaata tcctcgcttc cttcgcgctc attggaagtt tttaaagacg gtagttaaca agcttttcga gtttatgcac gagactcacc ctggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnraagta tgagagcggt aaagaaagaa acattgactt tgcaagagac ctttatagag aaatccgaag atcctcaaat cgttatgcaa aacttcattc ccccattgct tgaggcggtg ctcattgatt acaagaacaa cattccagac gcacgcgacc cagaagtgtt gtctttaatg actgttatca taaacaaatt caagcaagga ttaaacgacc agatacctcc tatcttggct gctgtgtttg agtgcacgct gcaaatgatt acacaaaact ttgaggacta ccccgtctgc cgagtgaact ttttcaacct catccgcgcc atcaataccc actgcttccc tgccttcttc caaattccac cagaaacctt caagctcatc atcgacagca ttgtgtgggc cttcaagcac accatgcgaa acattgctga gacgggactc aacatcctgt tagagctgtt gaagaacatt gcggcctctg gggaggaggt ggcccaagga ttctacaagg cctattttat caaccttgtg caggacatct tctacgtctt gaccgacgct ttccacaaat caagtttttc tttgcaagct acaatactcg ctcgaatgtt ccacttggta gaaagtggtc aagtgcgagt gcctctgtgg gacacaacga ccaaccagga tcccgccatg aacaactctg ttttcttgcg aaaccatatg acccatctat tgctcaccgc ttttcctcat ctttccaaag aaactgtgca tcaattcgtg atgggcctct ttgagcggaa cacagacatc tccgcgttca agttgctgct tcgagacttt ttggtgcagc tcaaggaatt ttcggagcag gacaattcgg agctgttcct agaggagaag gagcagcagt tggccgaggc gcagagcgag cagctcaagc gagacatggc cattcctggc atggttgccc cgcacgatgc tcgagtgcaa gataactcac agatggaata a
Expected Protein Sequence
MAHHHHHHME RILNFDEPLD VKLLDQVVYT FYTGSGPQQA EAERVLAQLQ EHPQAWSRVD TILEYSESQS TKFLALSILE KVILYRWKSL PRAQCDGIRN YIVNLVIKLS SDAATMQKER FYLKKLNMIL VQIIKQEWPR NWETFIPELV GASQKNESLC ENNMEILKLL SEEVFDFSGG QLTQDKIKEL KTSFNKEFSL IYQLCEYILN TSQSPSLIEA TLKTLLRFLN WIPVGYIFQT KMVENLIFKF FPIAPFQNIT LQCLTEVVGI KMGEQNEGIF TQLFTGFMQQ LHNILPPSTN LAEVYENGTE EQQQFIQNLA IFFGVFFKNH LKVLESEGTH MLLLEAHNYL IEISQVTDSE VWKICLEYWT TLASSLYQEF PAAPSQVLML NSHPSQSNSP RRSFYAPIMS RLRKVIIQRM PKPEEVLIVE DENGEIVREV MKDSDAIILY KSMRETLIYL THLDYEDTQS IMLEKLNNQV NGQEWSWNNL NTLCWAIGSI SGAQSEEDEK RFLVTVIKEL LGLCEMKRGK DNKAVIASNI MYIVGQYPRF LRAHWKFLKT VVNKLFEFMH ETHPGVQDMA CDTFLKIAQK CRRKFVVCQI GETAPFVEEI LTDLPVIISD LEPQQIQTFY EAVGFMIHSH TDPKARKELV IKLMALPNST WTRIMEEAQR DVSYLKQTQT CKSITTILKT NVRAAKSLGH DYIYQLGTIF MDMLNVYKVY STEISNTVAS NGPNATKTSI VRSMRAVKKE TLTLQETFIE KSEDPQIVMQ NFIPPLLEAV LIDYKNNIPD ARDPEVLSLM TVIINKFKQG LNDQIPPILA AVFECTLQMI TQNFEDYPVC RVNFFNLIRA INTHCFPAFF QIPPETFKLI IDSIVWAFKH TMRNIAETGL NILLELLKNI AASGEEVAQG FYKAYFINLV QDIFYVLTDA FHKSSFSLQA TILARMFHLV ESGQVRVPLW DTTTNQDPAM NNSVFLRNHM THLLLTAFPH LSKETVHQFV MGLFERNTDI SAFKLLLRDF LVQLKEFSEQ DNSELFLEEK EQQLAEAQSE QLKRDMAIPG MVAPHDARVQ DNSQME
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac gcatactcaa ctttgatgag ccactcgatg tgaagttact cgatcaagtg gtttatacct tctacacggg ctctgggcca cagcaagcag aggccgaaag ggtacttgca cagttgcaag agcatccaca agcatggtcg agggtggaca ccatcctcga atactctgag agtcaaagca caaagtttct cgctctctcc attctggaga aggtcatcct ctatcgatgg aaaagcctgc cgagggcgca gtgtgatggc attcgtaact acattgtcaa tcttgtgatc aagctgtcct ctgacgctgc caccatgcag aaggagcggt tctacttgaa gaagttgaat atgatcctcg tgcagattat caaacaagag tggccgcgca attgggaaac cttcattccc gaactcgttg gagctagtca aaagaacgaa tcgttgtgcg agaacaacat ggagatacta aagctgctca gcgaggaagt gtttgacttc tcaggtggtc aactgactca agacaaaata aaggagttga agacttcgtt caacaaggag ttttctctca tctaccagct ttgtgaatac atactgaaca cctcacagag cccctccctt attgaggcca ccttgaaaac cttgctgcgg tttctcaact ggatccctgt aggctatatt ttccaaacaa agatggttga aaatctaata tttaagttct ttccaattgc gcctttccaa aatattactt tgcaatgtct gaccgaagtt gttggcatta aaatgggaga gcagaatgaa ggcattttta ctcagctgtt tacagggttt atgcagcaac ttcacaatat attgccacct tctacgaatc tcgctgaggt ttacgagaat ggcaccgagg agcaacagca gtttattcag aaccttgcca tcttctttgg cgtctttttc aagaatcacc tcaaggtatt ggagtcggaa ggcactcaca tgctcctgtt ggaagctcac aactacctta ttgaaatatc tcaagttacc gatagcgagg tgtggaagat ctgtctcgag tattggacca cactggcaag ttcactctac caagagtttc ctgccgctcc ttcacaggtt ctcatgttga acagccatcc ctctcaatcc aactcgccca gacgctcatt ctacgctcct attatgtctc gactacgtaa agttatcatt caacgcatgc ccaagcctga ggaagtcttg attgtagaag atgaaaacgg agagatcgtt agggaggtga tgaaggatag tgacgccatc atcttgtaca agagcatgag agagacgttg atctatttga cgcatcttga ttatgaagac actcaaagca tcatgctcga aaagctgaac aatcaggtga atggtcaaga atggtcctgg aacaacctca acactctctg ctgggccatt ggatccatta gcggagctca atctgaagag gacgagaaac gatttttggt tactgttatt aaggagcttt tgggcttgtg cgaaatgaaa cgaggcaaag ataataaggc agtcatcgca agcaacatta tgtatatagt tgggcaatat cctcgcttcc ttcgcgctca ttggaagttt ttaaagacgg tagttaacaa gcttttcgag tttatgcacg agactcaccc tggtgtacaa gacatggctt gtgatacttt cctcaagatt gcccaaaagt gtaggcggaa attcgtggtg tgtcaaattg gagaaacggc accttttgta gaggagattt tgaccgattt gcccgtcatt atttccgacc tcgaaccaca acagatacaa actttttacg aagcagttgg ttttatgatt cactcccaca ctgaccccaa agcacggaaa gagctcgtca ttaagttgat ggcattaccc aattcaacgt ggacacgaat tatggaagaa gcacaacggg atgtctctta cttgaagcaa acgcaaacat gtaagagcat caccacaata ctcaagacaa acgtccgcgc agctaagtca cttggccatg actacattta ccaattggga actatattca tggacatgct caatgtgtac aaagtttaca gcacggaaat ctccaacact gttgcgtcca atggtcccaa tgctacaaaa acgtctattg tccgaagtat gagagcggta aagaaagaaa cattgacttt gcaagagacc tttatagaga aatccgaaga tcctcaaatc gttatgcaaa acttcattcc cccattgctt gaggcggtgc tcattgatta caagaacaac attccagacg cacgcgaccc agaagtgttg tctttaatga ctgttatcat aaacaaattc aagcaaggat taaacgacca gatacctcct atcttggctg ctgtgtttga gtgcacgctg caaatgatta cacaaaactt tgaggactac cccgtctgcc gagtgaactt tttcaacctc atccgcgcca tcaataccca ctgcttccct gccttcttcc aaattccacc agaaaccttc aagctcatca tcgacagcat tgtgtgggcc ttcaagcaca ccatgcgaaa cattgctgag acgggactca acatcctgtt agagctgttg aagaacattg cggcctctgg ggaggaggtg gcccaaggat tctacaaggc ctattttatc aatctcgtgc aggacatctt ctacgtcttg accgacgctt tccacaaatc aagtttttct ttgcaagcta caatactcgc tcgaatgttc cacttggtag aaagtggtca agtgcgagtg cctctgtggg acacaacgac caaccaggat cctgccatga acaactccgt tttcttgcga aaccatatga cccatctatt gctcaccgct ttccctcatc tttccaaaga aaccgtgcat caattcgtga tgggcctctt tgagcggaac acagacatct ccgcgttcaa gttgctgctt cgagactttt tggtgcagct caaggaattt tcggagcagg acaattcgga gctgttccta gaggagaagg agcagcagtt ggccgaggcg cagagcgagc agctcaagcg agacatggcc attcctggca tggttgcccc gcacgatgct cgagtgcaag ataactcaca gatggaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at