BamaA.19955.b
DNA topoisomerase II
CENTER ID:
BamaA.19955.b
ORGANISM:
Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
unclassified
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
IIB
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
BamaA.19955.b.B2.GE43483
ATPase and transducer domains
info
111
525
BamaA.19955.b.B2.GE43483
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BamaA.19955.b.B2.PW38788
ATPase and transducer domains
info
111
525
PW38788
Structures
7L6S
DEPOSITED: 12/23/2020
DETERMINATION: XRay
CLONE: BamaA.19955.b.B2.GE43483
PROTEIN: BamaA.19955.b.B2.PW38788
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALRAAQCCR PGPHPIANAR SSTAHTQKPT ALSVLLRATH PRPPGRPPHR CHSSLLSRKA LGSPSHSKTN VSAFRFSSCR KHSPPTKAPS FFPQRRSFAT KRKMNGPSTA SSAADRAAKT PEELYQKKTP IEHVLLRPDT YLGSVLRTTE PGMWVYDPDS RRMVERECTY VPALYKIFDE ILVNAADNKQ RDPNTSTIEV NIDADTNTIS VFNDGRGIPV HVHKTEGMYL PEMLFGHLLT SSNYDDSEAK VTGGRNGYGA KLTNIYSKEF TVETVDCERG LRFQQTWRDN MSVREEPLIT PLSPEEKAHG DYTKITFRPD LSRLDSMHSL RDGDIIGVMS RRAFDVAACN EGLDVYLNGE KLPSGFKGYV QLYHNPDVVE DAFVFEQVND RWQIVVGPSL DGQFTQQSFV NSIHTRRGGT HVTYILDQLT KHIVASIRRD HPDLTKIVQP ALVRNHLSLF INALIENPSF DSQTKETLTT QPSRFGSKCK LSDEFLERVV QRTRIVEEVT RWAELKQKDA LNRASPSAMK AGTRIHGIPK LDDANNAGGP NAQQCTLILT EGDSAKSLAV AGLSVLGRDN YGVYPLKGKL LNVKCASHAQ LLQNKEIQEI RTILGLDLDR KYDESEAAEH GGNPFHGLRY GKVMLMTDQD HDGSHIKGLF ISFLHHFWPA LLKQNFVEEF VTPIVKATRS IGGGKKETQV FYTIPEYNQW RKEMAEQIAG TEEDSASTTD SKRSKKGSKD SSSSGKWKIK YYKGLGTNTT EEGKAYFKDL EKHRIAFRWD GEEDGQQLDM AFKKEKVQAR KDWLMTYATA HQDADDVFVD HSQKNISFSE FVNKELILFS HADNIRAIPS VIDGLKPGQR KVLYACFKRK LTNEIKVAQL AGYVSEHTAY HHGEASLHGT IINMAQDFPG SNNLPLLHPS GQFGTRLLGG KDAASPRYIF TRLSSFARAL YPEEDDPLLQ YVEEDGERVQ PLYYVPVIPT VLINGTVGLG TGWSTTIPPH NPLDIIQNIR SKLDGKEMQP MEPWFHGWNG IMKRYGSNKF ITKGRVEKLS NNVLRISELP AQTWTYTYKE ALEAMLKPRT KGARPIIKSF KEFHTENTVD FHVYMTREQM LTAERVGFDR QLSLHSSLSL SNMHLFDGNG RIRHYDSTLD IIEEFVPIRY RFYQRRKEFL EERLQKELIL LENQAKFLEL VISGQLKVVN EKKSSLLQRL KQLGLAKIYS AASSTASRQQ KEEGENEKEK EEESEGEGDE RGYDYLLRGS LWNLTQEKIE QSRAKVERRR EELRVLQGKR PEDLWLQDLD RLEALVRSFY SSS
NT Sequence
atggcgctgc gagcagctca gtgctgtagg cctggacctc atcccattgc aaacgcgcgt agtagtactg cccacacaca aaaaccgacc gcgctttccg tcctcttgcg tgccacacat cctcgtccac ctggacgccc accgcatcgc tgccactctt ctcttctctc ccgcaaagcg ctgggctcac cttcgcacag caagacaaac gtttctgctt tccgcttttc ttcttgtcgc aaacactctc ctcccaccaa agccccttct ttctttccgc aacgacgttc tttcgccacc aagaggaaga tgaacgggcc ttccactgcc tcctctgctg ctgatcgggc tgccaaaacg ccggaagagc tctaccagaa aaagacgccg attgagcatg tcctgctccg ccctgacacc tacttggggt cggtgctacg aacgaccgag ccgggcatgt gggtctacga cccggacagc aggaggatgg tggagaggga gtgcacctac gtgccggcgc tctacaagat ctttgatgag atactcgtca atgctgctga caacaagcaa cgggatccga acacatcaac cattgaagta aacatcgatg cagacaccaa cactatttcc gtcttcaatg atggccgtgg cattcccgta catgtgcata aaacggaagg aatgtatttg cccgagatgt tgtttggaca tctactaacc agctccaatt acgatgactc ggaggccaaa gttactggcg gtcgcaatgg ctatggagca aagctcacca acatttactc caaggagttt acagtggaaa cagtggactg tgaacgaggc ctcaggttcc aacaaacatg gcgtgacaac atgagcgtgc gagaagagcc ccttatcact ccgctctccc ccgaagagaa agcccacgga gactacacca agatcacctt ccgaccggat ttgtctcgct tagactcaat gcattctctg cgcgatggag atatcattgg agtgatgtcc agaagagctt tcgatgtggc tgcttgcaac gagggactgg atgtctattt gaatggagag aaattgccaa gcgggtttaa gggttacgtt caactgtacc acaatcctga tgtagttgaa gatgctttcg tgttcgaaca ggtcaatgat aggtggcaaa tcgtagtagg tccctctctt gacggccaat tcacacaaca aagctttgtc aacagcatcc atacgcgtcg tggtggcact cacgttacct acatcctcga ccaactgacc aagcacattg tggcctccat ccgtcgtgac catccagacc tcaccaaaat tgttcagcct gctcttgttc gcaaccatct ttcactcttc atcaacgctc tcattgagaa tccctccttt gactctcaaa ccaaagaaac attgaccact caacccagtc gtttcggctc caaatgcaag ttatccgatg aatttctgga gagagtagtt caacgcactc gcatcgtgga ggaggtaacc cgttgggcgg agctcaagca aaaggacgct ctcaatcgag caagtccctc tgcaatgaag gcaggcacca ggattcatgg tattccaaag ttggacgatg ccaacaatgc gggaggaccg aacgcgcagc aatgcaccct cattttgacg gagggcgact cggcaaagag tttggcagta gctgggcttt ctgttcttgg aagagacaat tatggagttt accctctcaa gggaaaacta ttgaatgtta aatgtgcaag ccacgcgcaa ctgttgcaaa acaaagagat tcaggaaatt cgaactattc tcggccttga tctggaccgc aagtatgacg aaagcgaggc tgcagaacac ggtggtaatc cttttcatgg tcttcggtat gggaaagtca tgctcatgac ggatcaggac catgatggct cgcacatcaa gggtctcttt ataagcttct tgcatcactt ttggccggct ttgttgaaac aaaactttgt ggaggagttt gtgacgccta tcgtgaaagc gacaagaagc attggtggtg gaaagaaaga aactcaagtg ttttacacta tcccagaata taaccagtgg aggaaagaaa tggctgaaca aatagcagga acagaagaag attctgcatc cacaacagat tccaaacggt ctaaaaaagg aagcaaagat tcctcgtctt ccggcaaatg gaagatcaaa tactacaaag gtttaggtac taacaccact gaagaaggta aagcctactt taaagatttg gaaaagcacc gcattgcctt tcgttgggat ggagaagagg acggacaaca attggacatg gccttcaaga aagaaaaagt gcaagctaga aaagactggt taatgactta tgccaccgct caccaggatg cagacgacgt gtttgtggat cactctcaaa agaatatctc cttttcggaa tttgtgaata aggagctgat tctgtttagc catgcggata acattcgcgc tattccgagc gttattgatg gcctcaaacc tggacaacgt aaagtgcttt atgcctgctt taaacgaaag cttaccaacg agatcaaagt ggcccaattg gcgggttatg tgtcagagca cacagcttat catcacgggg aggcctcctt acatggcacc atcatcaaca tggcacaaga tttccctggc tccaacaact tgcccctgct tcatcctagt ggtcagtttg gtacaagact tttgggagga aaggatgcag ccagtccacg ttacatcttt actcgtttgt cctccttcgc acgagctctc tatcctgaag aggacgaccc tttgttgcaa tacgtagagg aggacggtga acgcgtgcaa cccctctatt acgtgccggt cattcctaca gtgctcatca atggcactgt tggccttggc accggttgga gcactactat tccgcctcac aacccgctcg acatcattca aaacattcga agcaaattgg atggaaagga aatgcagcca atggaaccct ggttccacgg atggaatggc attatgaaac gttatggttc caacaagttt atcacaaaag gaagagttga aaagttaagc aacaatgttt tgcggatttc agaattacca gcgcaaacat ggacttatac ctacaaagaa gcgctagagg cgatgttgaa accgagaacc aaaggcgctc gacccattat caagtccttc aaagagtttc acaccgaaaa caccgttgat tttcacgttt atatgactag agagcaaatg ttaacggccg aacgagtagg cttcgaccgg caactctccc tccacagctc cctctccctt tccaacatgc acctctttga tgggaacggc aggatccgcc actacgactc cacgctagac ataatcgagg agttcgttcc gattcgctat cgtttctacc agcggagaaa agagtttttg gaggaacgtt tacaaaagga gctgatcctt ttggagaatc aggccaagtt tttggagtta gtcatctctg ggcagctcaa agtggtcaac gaaaagaaga gcagcttatt gcaacggctg aaacaactgg ggctggccaa gatttattct gcagcgagtt ccactgcgag ccgacaacag aaagaagagg gagagaacga gaaagagaaa gaagaagaaa gtgaaggaga gggagacgaa agaggctacg attatttgtt gcggggaagt ctttggaatt tgacgcagga aaagatcgag cagagcaggg ccaaagtgga gaggcggcgg gaggagttgc gggtgctaca ggggaagagg ccggaggacc tttggctaca ggacctggat cgactggaag cgctcgttcg ctctttctat tcttcttctt aa
Details for BamaA.19955.b.B2.GE43483
HARVESTED ON:
11/19/2019
SEQUENCED ON:
12/3/2019
EXPECTED MW:
48kDa
OBSERVED MW:
48kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
98
Details for BamaA.19955.b.B2.PW38788
PURIFICATION DATe:
1/18/2020
CONCENTRATION:
18mg/ml
OBSERVED MW:
48kDa
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:
BL 21 (DE3) Rosetta
VIAL COUNT (approx.):
6
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
98