BamaA.19955.b
DNA topoisomerase II
| CENTER ID: | BamaA.19955.b |
| ORGANISM: | Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BamaA.19955.b.B2.GE43483 | ATPase and transducer domains | 111 | 525 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BamaA.19955.b.B2.PW38788 | ATPase and transducer domains | 111 | 525 |
Structures
7L6S
DEPOSITED: 12/23/2020
DETERMINATION: XRay
CLONE: BamaA.19955.b.B2.GE43483
PROTEIN: BamaA.19955.b.B2.PW38788
External Resources
| RESOURCE | REFERENCE ID |
|---|
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALRAAQCCR PGPHPIANAR SSTAHTQKPT ALSVLLRATH PRPPGRPPHR CHSSLLSRKA LGSPSHSKTN VSAFRFSSCR KHSPPTKAPS FFPQRRSFAT KRKMNGPSTA SSAADRAAKT PEELYQKKTP IEHVLLRPDT YLGSVLRTTE PGMWVYDPDS RRMVERECTY VPALYKIFDE ILVNAADNKQ RDPNTSTIEV NIDADTNTIS VFNDGRGIPV HVHKTEGMYL PEMLFGHLLT SSNYDDSEAK VTGGRNGYGA KLTNIYSKEF TVETVDCERG LRFQQTWRDN MSVREEPLIT PLSPEEKAHG DYTKITFRPD LSRLDSMHSL RDGDIIGVMS RRAFDVAACN EGLDVYLNGE KLPSGFKGYV QLYHNPDVVE DAFVFEQVND RWQIVVGPSL DGQFTQQSFV NSIHTRRGGT HVTYILDQLT KHIVASIRRD HPDLTKIVQP ALVRNHLSLF INALIENPSF DSQTKETLTT QPSRFGSKCK LSDEFLERVV QRTRIVEEVT RWAELKQKDA LNRASPSAMK AGTRIHGIPK LDDANNAGGP NAQQCTLILT EGDSAKSLAV AGLSVLGRDN YGVYPLKGKL LNVKCASHAQ LLQNKEIQEI RTILGLDLDR KYDESEAAEH GGNPFHGLRY GKVMLMTDQD HDGSHIKGLF ISFLHHFWPA LLKQNFVEEF VTPIVKATRS IGGGKKETQV FYTIPEYNQW RKEMAEQIAG TEEDSASTTD SKRSKKGSKD SSSSGKWKIK YYKGLGTNTT EEGKAYFKDL EKHRIAFRWD GEEDGQQLDM AFKKEKVQAR KDWLMTYATA HQDADDVFVD HSQKNISFSE FVNKELILFS HADNIRAIPS VIDGLKPGQR KVLYACFKRK LTNEIKVAQL AGYVSEHTAY HHGEASLHGT IINMAQDFPG SNNLPLLHPS GQFGTRLLGG KDAASPRYIF TRLSSFARAL YPEEDDPLLQ YVEEDGERVQ PLYYVPVIPT VLINGTVGLG TGWSTTIPPH NPLDIIQNIR SKLDGKEMQP MEPWFHGWNG IMKRYGSNKF ITKGRVEKLS NNVLRISELP AQTWTYTYKE ALEAMLKPRT KGARPIIKSF KEFHTENTVD FHVYMTREQM LTAERVGFDR QLSLHSSLSL SNMHLFDGNG RIRHYDSTLD IIEEFVPIRY RFYQRRKEFL EERLQKELIL LENQAKFLEL VISGQLKVVN EKKSSLLQRL KQLGLAKIYS AASSTASRQQ KEEGENEKEK EEESEGEGDE RGYDYLLRGS LWNLTQEKIE QSRAKVERRR EELRVLQGKR PEDLWLQDLD RLEALVRSFY SSS
NT Sequence
atggcgctgc gagcagctca gtgctgtagg cctggacctc atcccattgc aaacgcgcgt agtagtactg cccacacaca aaaaccgacc gcgctttccg tcctcttgcg tgccacacat cctcgtccac ctggacgccc accgcatcgc tgccactctt ctcttctctc ccgcaaagcg ctgggctcac cttcgcacag caagacaaac gtttctgctt tccgcttttc ttcttgtcgc aaacactctc ctcccaccaa agccccttct ttctttccgc aacgacgttc tttcgccacc aagaggaaga tgaacgggcc ttccactgcc tcctctgctg ctgatcgggc tgccaaaacg ccggaagagc tctaccagaa aaagacgccg attgagcatg tcctgctccg ccctgacacc tacttggggt cggtgctacg aacgaccgag ccgggcatgt gggtctacga cccggacagc aggaggatgg tggagaggga gtgcacctac gtgccggcgc tctacaagat ctttgatgag atactcgtca atgctgctga caacaagcaa cgggatccga acacatcaac cattgaagta aacatcgatg cagacaccaa cactatttcc gtcttcaatg atggccgtgg cattcccgta catgtgcata aaacggaagg aatgtatttg cccgagatgt tgtttggaca tctactaacc agctccaatt acgatgactc ggaggccaaa gttactggcg gtcgcaatgg ctatggagca aagctcacca acatttactc caaggagttt acagtggaaa cagtggactg tgaacgaggc ctcaggttcc aacaaacatg gcgtgacaac atgagcgtgc gagaagagcc ccttatcact ccgctctccc ccgaagagaa agcccacgga gactacacca agatcacctt ccgaccggat ttgtctcgct tagactcaat gcattctctg cgcgatggag atatcattgg agtgatgtcc agaagagctt tcgatgtggc tgcttgcaac gagggactgg atgtctattt gaatggagag aaattgccaa gcgggtttaa gggttacgtt caactgtacc acaatcctga tgtagttgaa gatgctttcg tgttcgaaca ggtcaatgat aggtggcaaa tcgtagtagg tccctctctt gacggccaat tcacacaaca aagctttgtc aacagcatcc atacgcgtcg tggtggcact cacgttacct acatcctcga ccaactgacc aagcacattg tggcctccat ccgtcgtgac catccagacc tcaccaaaat tgttcagcct gctcttgttc gcaaccatct ttcactcttc atcaacgctc tcattgagaa tccctccttt gactctcaaa ccaaagaaac attgaccact caacccagtc gtttcggctc caaatgcaag ttatccgatg aatttctgga gagagtagtt caacgcactc gcatcgtgga ggaggtaacc cgttgggcgg agctcaagca aaaggacgct ctcaatcgag caagtccctc tgcaatgaag gcaggcacca ggattcatgg tattccaaag ttggacgatg ccaacaatgc gggaggaccg aacgcgcagc aatgcaccct cattttgacg gagggcgact cggcaaagag tttggcagta gctgggcttt ctgttcttgg aagagacaat tatggagttt accctctcaa gggaaaacta ttgaatgtta aatgtgcaag ccacgcgcaa ctgttgcaaa acaaagagat tcaggaaatt cgaactattc tcggccttga tctggaccgc aagtatgacg aaagcgaggc tgcagaacac ggtggtaatc cttttcatgg tcttcggtat gggaaagtca tgctcatgac ggatcaggac catgatggct cgcacatcaa gggtctcttt ataagcttct tgcatcactt ttggccggct ttgttgaaac aaaactttgt ggaggagttt gtgacgccta tcgtgaaagc gacaagaagc attggtggtg gaaagaaaga aactcaagtg ttttacacta tcccagaata taaccagtgg aggaaagaaa tggctgaaca aatagcagga acagaagaag attctgcatc cacaacagat tccaaacggt ctaaaaaagg aagcaaagat tcctcgtctt ccggcaaatg gaagatcaaa tactacaaag gtttaggtac taacaccact gaagaaggta aagcctactt taaagatttg gaaaagcacc gcattgcctt tcgttgggat ggagaagagg acggacaaca attggacatg gccttcaaga aagaaaaagt gcaagctaga aaagactggt taatgactta tgccaccgct caccaggatg cagacgacgt gtttgtggat cactctcaaa agaatatctc cttttcggaa tttgtgaata aggagctgat tctgtttagc catgcggata acattcgcgc tattccgagc gttattgatg gcctcaaacc tggacaacgt aaagtgcttt atgcctgctt taaacgaaag cttaccaacg agatcaaagt ggcccaattg gcgggttatg tgtcagagca cacagcttat catcacgggg aggcctcctt acatggcacc atcatcaaca tggcacaaga tttccctggc tccaacaact tgcccctgct tcatcctagt ggtcagtttg gtacaagact tttgggagga aaggatgcag ccagtccacg ttacatcttt actcgtttgt cctccttcgc acgagctctc tatcctgaag aggacgaccc tttgttgcaa tacgtagagg aggacggtga acgcgtgcaa cccctctatt acgtgccggt cattcctaca gtgctcatca atggcactgt tggccttggc accggttgga gcactactat tccgcctcac aacccgctcg acatcattca aaacattcga agcaaattgg atggaaagga aatgcagcca atggaaccct ggttccacgg atggaatggc attatgaaac gttatggttc caacaagttt atcacaaaag gaagagttga aaagttaagc aacaatgttt tgcggatttc agaattacca gcgcaaacat ggacttatac ctacaaagaa gcgctagagg cgatgttgaa accgagaacc aaaggcgctc gacccattat caagtccttc aaagagtttc acaccgaaaa caccgttgat tttcacgttt atatgactag agagcaaatg ttaacggccg aacgagtagg cttcgaccgg caactctccc tccacagctc cctctccctt tccaacatgc acctctttga tgggaacggc aggatccgcc actacgactc cacgctagac ataatcgagg agttcgttcc gattcgctat cgtttctacc agcggagaaa agagtttttg gaggaacgtt tacaaaagga gctgatcctt ttggagaatc aggccaagtt tttggagtta gtcatctctg ggcagctcaa agtggtcaac gaaaagaaga gcagcttatt gcaacggctg aaacaactgg ggctggccaa gatttattct gcagcgagtt ccactgcgag ccgacaacag aaagaagagg gagagaacga gaaagagaaa gaagaagaaa gtgaaggaga gggagacgaa agaggctacg attatttgtt gcggggaagt ctttggaatt tgacgcagga aaagatcgag cagagcaggg ccaaagtgga gaggcggcgg gaggagttgc gggtgctaca ggggaagagg ccggaggacc tttggctaca ggacctggat cgactggaag cgctcgttcg ctctttctat tcttcttctt aa
Details for BamaA.19955.b.B2.GE43483
| HARVESTED ON: | 11/19/2019 |
| SEQUENCED ON: | 12/3/2019 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMS SAADRAAKTP EELYQKKTPI EHVLLRPDTY LGSVLRTTEP GMWVYDPDSR RMVERECTYV PALYKIFDEI LVNAADNKQR DPNTSTIEVN IDADTNTISV FNDGRGIPVH VHKTEGMYLP EMLFGHLLTS SNYDDSEAKV TGGRNGYGAK LTNIYSKEFT VETVDCERGL RFQQTWRDNM SVREEPLITP LSPEEKAHGD YTKITFRPDL SRLDSMHSLR DGDIIGVMSR RAFDVAACNE GLDVYLNGEK LPSGFKGYVQ LYHNPDVVED AFVFEQVNDR WQIVVGPSLD GQFTQQSFVN SIHTRRGGTH VTYILDQLTK HIVASIRRDH PDLTKIVQPA LVRNHLSLFI NALIENPSFD SQTKETLTTQ PSRFGSKCKL SDEFLERVVQ RTRIVEEVTR WAELKQKD
Validated NT Sequence
gcgtcctttt gcttgagctc cgcccaacgg gttacctcct ccacgatgcg agtgcgttga actactctct ccagaaattc atcggataac ttgcatttgg agccgaaacg actgggttga gtggtcaatg tttctttggt ttgagagtca aaggagggat tctcaatgag agcgttgatg aagagtgaaa gatggttgcg aacaagagca ggctgaacaa ttttggtgag gtctggatgg tcacgacgga tggaggccac aatgtgcttg gtcagttggt cgaggatgta ggtaacgtga gtgccaccac gacgcgtatg gatgctgttg acaaagcttt gttgtgtgaa ttggccgtca agagagggac ctactacgat ttgccaccta tcattgacct gttcgaacac gaaagcatct tcaactacat caggattgtg gtacagttga acgtaaccct taaacccgct tggcaatttc tctccattca aatagacatc cagtccctcg ttgcaagcag ccacatcgaa agctcttctg gacatcactc caatgatatc tccatcgcgc agagaatgca ttgagtctaa gcgagacaaa tccggtcgga aggtgatctt ggtgtagtct ccgtgggctt tctcttcggg ggagagcgga gtgataaggg gctcttctcg cacgctcatg ttgtcacgcc atgtttgttg gaacctgagg cctcgttcac agtccactgt ttccactgta aactccttgg agtaaatgtt ggtgagcttt gctccatagc cattgcgacc gccagtaact ttggcctccg agtcatcgta attggagctg gttagtagat gtccaaacaa catctcgggc aaatacattc cttccgtttt atgcacatgt acgggaatgc cacggccatc attgaagacg gaaatagtgt tggtgtctgc atctatgttt acttcaatgg ttgacgtgtt cggatcccgt tgcttgttgt cagcagcatt gacgagtatc tcatcaaaga tcttgtagag cgccggcacg taggtgcact ccctctccac catcctcctg ctgtccgggt cgtagaccca catgcccggc tcggtcgttc gtagcaccga ccccaagtag gtgtcagggc ggagcaggac atgctcaatc ggcgtctttt tctggtagag ctcttccggc gttttggcag cccgatcagc agcagaggac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHSS AADRAAKTPE ELYQKKTPIE HVLLRPDTYL GSVLRTTEPG MWVYDPDSRR MVERECTYVP ALYKIFDEIL VNAADNKQRD PNTSTIEVNI DADTNTISVF NDGRGIPVHV HKTEGMYLPE MLFGHLLTSS NYDDSEAKVT GGRNGYGAKL TNIYSKEFTV ETVDCERGLR FQQTWRDNMS VREEPLITPL SPEEKAHGDY TKITFRPDLS RLDSMHSLRD GDIIGVMSRR AFDVAACNEG LDVYLNGEKL PSGFKGYVQL YHNPDVVEDA FVFEQVNDRW QIVVGPSLDG QFTQQSFVNS IHTRRGGTHV TYILDQLTKH IVASIRRDHP DLTKIVQPAL VRNHLSLFIN ALIENPSFDS QTKETLTTQP SRFGSKCKLS DEFLERVVQR TRIVEEVTRW AELKQKDALN RAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcctctg ctgctgatcg ggctgccaaa acgccggaag agctctacca gaaaaagacg ccgattgagc atgtcctgct ccgccctgac acctacttgg ggtcggtgct acgaacgacc gagccgggca tgtgggtcta cgacccggac agcaggagga tggtggagag ggagtgcacc tacgtgccgg cgctctacaa gatctttgat gagatactcg tcaatgctgc tgacaacaag caacgggatc cgaacacatc aaccattgaa gtaaacatcg atgcagacac caacactatt tccgtcttca atgatggccg tggcattccc gtacatgtgc ataaaacgga aggaatgtat ttgcccgaga tgttgtttgg acatctacta accagctcca attacgatga ctcggaggcc aaagttactg gcggtcgcaa tggctatgga gcaaagctca ccaacattta ctccaaggag tttacagtgg aaacagtgga ctgtgaacga ggcctcaggt tccaacaaac atggcgtgac aacatgagcg tgcgagaaga gccccttatc actccgctct cccccgaaga gaaagcccac ggagactaca ccaagatcac cttccgaccg gatttgtctc gcttagactc aatgcattct ctgcgcgatg gagatatcat tggagtgatg tccagaagag ctttcgatgt ggctgcttgc aacgagggac tggatgtcta tttgaatgga gagaaattgc caagcgggtt taagggttac gttcaactgt accacaatcc tgatgtagtt gaagatgctt tcgtgttcga acaggtcaat gataggtggc aaatcgtagt aggtccctct cttgacggcc aattcacaca acaaagcttt gtcaacagca tccatacgcg tcgtggtggc actcacgtta cctacatcct cgaccaactg accaagcaca ttgtggcctc catccgtcgt gaccatccag acctcaccaa aattgttcag cctgctcttg ttcgcaacca tctttcactc ttcatcaacg ctctcattga gaatccctcc tttgactctc aaaccaaaga aacattgacc actcaaccca gtcgtttcgg ctccaaatgc aagttatccg atgaatttct ggagagagta gttcaacgca ctcgcatcgt ggaggaggta acccgttggg cggagctcaa gcaaaaggac gctctcaatc gagcaagttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BamaA.19955.b.B2.PW38788
| PURIFICATION DATe: | 1/18/2020 |
| CONCENTRATION: | 18mg/ml |
| OBSERVED MW: | 48kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS SAADRAAKTP EELYQKKTPI EHVLLRPDTY LGSVLRTTEP GMWVYDPDSR RMVERECTYV PALYKIFDEI LVNAADNKQR DPNTSTIEVN IDADTNTISV FNDGRGIPVH VHKTEGMYLP EMLFGHLLTS SNYDDSEAKV TGGRNGYGAK LTNIYSKEFT VETVDCERGL RFQQTWRDNM SVREEPLITP LSPEEKAHGD YTKITFRPDL SRLDSMHSLR DGDIIGVMSR RAFDVAACNE GLDVYLNGEK LPSGFKGYVQ LYHNPDVVED AFVFEQVNDR WQIVVGPSLD GQFTQQSFVN SIHTRRGGTH VTYILDQLTK HIVASIRRDH PDLTKIVQPA LVRNHLSLFI NALIENPSFD SQTKETLTTQ PSRFGSKCKL SDEFLERVVQ RTRIVEEVTR WAELKQKD
Validated NT Sequence
gcgtcctttt gcttgagctc cgcccaacgg gttacctcct ccacgatgcg agtgcgttga actactctct ccagaaattc atcggataac ttgcatttgg agccgaaacg actgggttga gtggtcaatg tttctttggt ttgagagtca aaggagggat tctcaatgag agcgttgatg aagagtgaaa gatggttgcg aacaagagca ggctgaacaa ttttggtgag gtctggatgg tcacgacgga tggaggccac aatgtgcttg gtcagttggt cgaggatgta ggtaacgtga gtgccaccac gacgcgtatg gatgctgttg acaaagcttt gttgtgtgaa ttggccgtca agagagggac ctactacgat ttgccaccta tcattgacct gttcgaacac gaaagcatct tcaactacat caggattgtg gtacagttga acgtaaccct taaacccgct tggcaatttc tctccattca aatagacatc cagtccctcg ttgcaagcag ccacatcgaa agctcttctg gacatcactc caatgatatc tccatcgcgc agagaatgca ttgagtctaa gcgagacaaa tccggtcgga aggtgatctt ggtgtagtct ccgtgggctt tctcttcggg ggagagcgga gtgataaggg gctcttctcg cacgctcatg ttgtcacgcc atgtttgttg gaacctgagg cctcgttcac agtccactgt ttccactgta aactccttgg agtaaatgtt ggtgagcttt gctccatagc cattgcgacc gccagtaact ttggcctccg agtcatcgta attggagctg gttagtagat gtccaaacaa catctcgggc aaatacattc cttccgtttt atgcacatgt acgggaatgc cacggccatc attgaagacg gaaatagtgt tggtgtctgc atctatgttt acttcaatgg ttgacgtgtt cggatcccgt tgcttgttgt cagcagcatt gacgagtatc tcatcaaaga tcttgtagag cgccggcacg taggtgcact ccctctccac catcctcctg ctgtccgggt cgtagaccca catgcccggc tcggtcgttc gtagcaccga ccccaagtag gtgtcagggc ggagcaggac atgctcaatc ggcgtctttt tctggtagag ctcttccggc gttttggcag cccgatcagc agcagaggac atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHSS AADRAAKTPE ELYQKKTPIE HVLLRPDTYL GSVLRTTEPG MWVYDPDSRR MVERECTYVP ALYKIFDEIL VNAADNKQRD PNTSTIEVNI DADTNTISVF NDGRGIPVHV HKTEGMYLPE MLFGHLLTSS NYDDSEAKVT GGRNGYGAKL TNIYSKEFTV ETVDCERGLR FQQTWRDNMS VREEPLITPL SPEEKAHGDY TKITFRPDLS RLDSMHSLRD GDIIGVMSRR AFDVAACNEG LDVYLNGEKL PSGFKGYVQL YHNPDVVEDA FVFEQVNDRW QIVVGPSLDG QFTQQSFVNS IHTRRGGTHV TYILDQLTKH IVASIRRDHP DLTKIVQPAL VRNHLSLFIN ALIENPSFDS QTKETLTTQP SRFGSKCKLS DEFLERVVQR TRIVEEVTRW AELKQKDALN RAS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCCTCTG CTGCTGATCG GGCTGCCAAA ACGCCGGAAG AGCTCTACCA GAAAAAGACG CCGATTGAGC ATGTCCTGCT CCGCCCTGAC ACCTACTTGG GGTCGGTGCT ACGAACGACC GAGCCGGGCA TGTGGGTCTA CGACCCGGAC AGCAGGAGGA TGGTGGAGAG GGAGTGCACC TACGTGCCGG CGCTCTACAA GATCTTTGAT GAGATACTCG TCAATGCTGC TGACAACAAG CAACGGGATC CGAACACATC AACCATTGAA GTAAACATCG ATGCAGACAC CAACACTATT TCCGTCTTCA ATGATGGCCG TGGCATTCCC GTACATGTGC ATAAAACGGA AGGAATGTAT TTGCCCGAGA TGTTGTTTGG ACATCTACTA ACCAGCTCCA ATTACGATGA CTCGGAGGCC AAAGTTACTG GCGGTCGCAA TGGCTATGGA GCAAAGCTCA CCAACATTTA CTCCAAGGAG TTTACAGTGG AAACAGTGGA CTGTGAACGA GGCCTCAGGT TCCAACAAAC ATGGCGTGAC AACATGAGCG TGCGAGAAGA GCCCCTTATC ACTCCGCTCT CCCCCGAAGA GAAAGCCCAC GGAGACTACA CCAAGATCAC CTTCCGACCG GATTTGTCTC GCTTAGACTC AATGCATTCT CTGCGCGATG GAGATATCAT TGGAGTGATG TCCAGAAGAG CTTTCGATGT GGCTGCTTGC AACGAGGGAC TGGATGTCTA TTTGAATGGA GAGAAATTGC CAAGCGGGTT TAAGGGTTAC GTTCAACTGT ACCACAATCC TGATGTAGTT GAAGATGCTT TCGTGTTCGA ACAGGTCAAT GATAGGTGGC AAATCGTAGT AGGTCCCTCT CTTGACGGCC AATTCACACA ACAAAGCTTT GTCAACAGCA TCCATACGCG TCGTGGTGGC ACTCACGTTA CCTACATCCT CGACCAACTG ACCAAGCACA TTGTGGCCTC CATCCGTCGT GACCATCCAG ACCTCACCAA AATTGTTCAG CCTGCTCTTG TTCGCAACCA TCTTTCACTC TTCATCAACG CTCTCATTGA GAATCCCTCC TTTGACTCTC AAACCAAAGA AACATTGACC ACTCAACCCA GTCGTTTCGG CTCCAAATGC AAGTTATCCG ATGAATTTCT GGAGAGAGTA GTTCAACGCA CTCGCATCGT GGAGGAGGTA ACCCGTTGGG CGGAGCTCAA GCAAAAGGAC GCTCTCAATC GAGCAAGTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT