BamaA.19955.a

DNA topoisomerase II

CENTER ID: BamaA.19955.a
ORGANISM: Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamaA.19955.a.B2.GE43481 ATPase and transducer domains 93 490
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamaA.19955.a.B2.PW38787 ATPase and transducer domains 93 490

External Resources

RESOURCE REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDESDFDFS EVDDDSGDDW GGSPAPVKKA AAKPTTKAVA KTTAAAPTKR RAPAKAKATS SVSASALAKK AKTATANYDA SRPSLAPPEC PPEEDTEAWM AYQRMSPHDH ILIRPDTYVG SVERTEATMW VYDDGQGMTL RPISYVPGLY KIFDEILVNA ADNYQRDKSM SEIRVTINAE NNTIEVWNDG EGIPVEMHKK EDMYVPELIF GHLLTSSNYD DEKEKVTGGR NGYGAKLANI FSTKFSIETV DSKRKKKFYM EWTDNMRTKG KQKITTISAS KDYTKVTFTP DLEKFGMTEF DEDTVSLLTK RVYDVAGCNS KLKVKLNGKL LKISSFEKYV DLYFQGEKLP HIFEKNDRWE VCVSVSKDSE FRQVSFVNSI CTTQGGSHVK YIVDKLVKQL IPAIKKKAKV STIKPAQVKA HLWVFINCLI VNPAFNSQTK DTLTSQPKSF GSTCDLSENF IKQVVNKTAI ATYTGEFAKM KENQELQKKG GSKKTRIAGI TKLDDANEAG GRNADKCTLI LTEGDSAKTL AVAGLSVVGR NRYGVFPLKG KPLNVRDATH KQVVSNVEFT NIKQILGLKH GADYSSGTKG LRYGKLMIMA DQDHDGSHIK GLVINFLHYY WPSLLKIPGF VTEFITPIVK ATNGKQVISF FTQTEYESWK QANNNAKNWK IKYYKGLGTS TPVEGKQYFK AINRHKIDFK WVNEEDGDMI ELAFSKSRVN DRKTWLSNFK LGTCLDSNVS QIRYADFINK ELIHFSIADC ARSIPCFVDG LKPGQRKILF ACFLRNLRKE MKVAQLTGYV GSVSAYHHGE ESLAKTIVGM AQNFCGSNNI NLLVPAGSFG SRLQGGKDAA SPRYIHTLLC DITRKIFHPD DDNLLEYLDD DGFSIEPRFY VPIIPMVLIN GADGIGTGWS SFVPNYNPRD VITNIQRLIR GQEMQTMHPW YRGFEGIITE DIANKTPRYK NTGIFRRPDP ETLEIVELPI GMWTQKYKKF LEELRSGTKK HPDPVIKDFD EHHTHKKVHF VIYLLPETAK KLTTNDDIEK FFKLSDYLSI NNMHLFDEQL KIRRWDTAAE LMQDFYEIRL DYYERRKKFM ENLLERKLSK LDNQVRFILA VVEERLVVNK RKKAELLAQL EKEGYDKFSN DDELGTNAWK RRKKQAKKKE RPEDDEEDEE EEKKNKKKDK KKTENGYDYL LSMKLWNLTM EKVEELIAQR DKKKEELEEL KRTGVTTLWE RDLQALMESY EAFEQAWEEE EEEELGDGSK NNRRRRKPAA APRKRAVSAT KKAKGKDLMD DEEDDDDFTD DSWKYNENVA PNKKTTTTAA AKATTTTTRK KQGTLDGVLQ KKTTATTTTA TKKATIGRAA AAAKKTISLV DEKDEDEDDD DMEEEKKVTG MTSKKRQLAP KQAASSTVKM EIADEEEEEA EDKDEDEEFL SLADRILKKK SGGAASTKTT TTKKKASVFD SLSDLDSSDD EDSKPVVVAP KRGAAAAKKQ ATTSKTTAKT TVARPAKRKA AATNDADDGE SSSSTSTTAG RGAKKAKITD FMQSAASKRK AAPPKKEEIE EEEEEDDEEE EEEEEEEEKP QAPKAQAPPS RRLKRADSQL KLAKGPSGPV SLTRGLSISD FYTNYAGMHD QIHAAPRDSF SNPQGREYDL GHEGAFGQFS VLVGLFCPDQ LSEETFMKDA GKALGEKGFR VYVTTSEEGF AKNLPANDIG FVISTRTVSE HTPAVAEACW RHHQSGRGLA VWAENAPYFA TANEVLKKLS PGTVITGYDS GGKSLSVGDA NKRQQFGRHL LTSGITNLFE GITISYPESI GENLKILATS SDNHPCMLCA DYPDIKDHEG RIVVDCGFTK LFCHWDTAGT ARYVRNVCVW LLGLEHRLKL GMPLQGPMKL DTDGGSSSSS SSSSSATSKA E
NT Sequence
atgtcggacg agtctgattt tgatttcagt gaggtggacg acgattctgg agatgactgg ggaggaagcc ctgcgcccgt caaaaaggct gctgcaaagc caactactaa agccgttgca aagaccaccg ctgcagctcc aaccaagcga agagcgcctg ccaaggcaaa ggcaacttcc tctgtttccg cctctgctct tgccaaaaag gccaaaacgg ccaccgccaa ctacgatgcc tctcgcccca gcctcgctcc tcccgaatgc cctcctgagg aagacaccga agcatggatg gcctaccaac gcatgtcacc tcatgaccac atcctcattc gtcctgacac ctacgtgggc tctgtggaga gaacggaagc cacgatgtgg gtctatgatg atggacaggg catgaccttg cgtcccattt cctatgttcc tggtctctac aagatctttg acgagatcct ggtgaacgcg gcggacaact accaaagaga caagagcatg tccgagatac gagttaccat caacgcggag aacaacacca tcgaagtttg gaacgacggt gagggtattc ctgtggagat gcacaaaaaa gaggacatgt atgttccaga gcttattttc ggtcacctct tgacctcttc gaactacgac gacgaaaaag aaaaggtaac tggtggaaga aacggttatg gcgcgaagct ggccaacatc ttctccacca agttttccat cgagacggtg gactcaaagc gaaagaaaaa gttctacatg gaatggactg ataacatgag gacgaaagga aagcagaaaa tcactaccat ttccgcctcc aaagactaca ccaaggtcac tttcacgccc gatcttgaga agtttgggat gaccgagttt gatgaagata cggttagtct ccttaccaag cgtgtgtacg atgttgctgg ctgcaactcc aagctcaaag tcaagctcaa tggaaagctt ttgaagatca gttcatttga gaaatatgta gatttgtact ttcaaggaga gaagctccct catatctttg agaaaaacga tcgatgggag gtctgcgtga gcgtgagcaa agactccgag tttcgtcaag ttagctttgt caacagcatt tgcaccactc aaggaggcag ccacgtgaaa tacattgtgg acaagttggt aaagcaactc attccggcca tcaagaagaa agccaaggtc tctaccatta agcccgctca ggttaaggcg catttgtggg tgtttatcaa ttgcctcatt gtcaatcctg cgttcaactc ccaaacgaag gacacgttga cctcgcaacc aaagagcttt gggtcaacct gtgacctctc tgagaacttt atcaagcaag tggtaaacaa gactgcaatt gcgacataca cgggagagtt tgcgaagatg aaggaaaacc aagaactgca aaagaaaggc ggtagcaaga aaaccagaat cgctggtatc accaagctag acgatgccaa cgaggccggt ggccgaaacg ccgacaagtg cactttgatt cttactgagg gagactctgc caagactctt gctgtagctg gtctttccgt tgttggtcgc aatagatacg gagtgtttcc tttgaaagga aagcctctca acgttcgtga tgcgacgcac aagcaggttg taagcaacgt cgaatttacc aacatcaaac aaattcttgg tttgaagcat ggcgctgact acagcagcgg aacgaagggc ctgcgttatg gcaagctgat gataatggct gaccaggatc acgacggatc gcatatcaaa gggttagtca tcaactttct tcactattac tggccatcac tgttgaagat tcccggcttt gtgaccgagt tcatcacccc catcgtgaaa gcgaccaacg gcaaacaagt catttccttc ttcacccaga ctgaatacga gagctggaag caagccaaca acaacgctaa gaattggaaa atcaagtact acaagggtct aggaacgagc acgccggtgg agggaaaaca atacttcaag gccattaacc gccacaagat tgactttaaa tgggtgaatg aggaggatgg agacatgatt gagctggcct ttagcaagag ccgcgtcaat gatcgtaaga catggttgtc caactttaag ttaggaacct gtttggattc aaatgtgtct cagattcgtt atgccgattt catcaacaaa gagctgatcc acttttccat cgcggattgc gctcgttcca tcccttgctt tgtggatgga cttaagcccg gccaaaggaa aattctcttt gcctgcttcc ttagaaacct aagaaaggag atgaaggtgg cccaactgac gggttacgtt ggctctgtgt cggcctacca tcatggagag gagagcttgg ccaagacaat cgtgggtatg gcccaaaact tttgtggctc taacaacatc aaccttctcg tacctgcggg cagctttggc agtcgcttgc agggcggtaa agatgcggcc agtcctaggt acattcacac ccttctctgt gacataaccc gcaagatctt tcatccggac gacgacaacc tcctcgagta cctcgacgat gacggcttct ccatagagcc ccgcttctac gtgcctatca tccccatggt cctcatcaac ggcgctgacg gcatcggtac cggctggagc tcctttgtgc ccaactataa cccgcgcgac gtcatcacca acatccagag actcatccgt ggccaggaga tgcagaccat gcacccttgg tacagaggct ttgagggcat cattaccgaa gacatcgcca acaaaacgcc gagatacaag aacacgggca tctttagaag gccggatccg gagacgttgg agattgtaga gctgccgatc ggcatgtgga ctcaaaagta caagaagttt ttggaggagt tgcgctctgg cacgaagaaa caccctgatc ctgttattaa ggacttcgac gagcatcaca cccacaaaaa ggtccacttt gtaatctacc tcctgccaga aaccgcaaag aagctgacga ccaacgatga cattgaaaag ttcttcaaac tcagcgacta tctcagcata aataacatgc acctctttga cgagcagctc aagattcgcc gatgggacac cgcagcggag ctcatgcaag acttttacga gattcgtttg gactattacg agcgccgcaa aaagtttatg gaaaaccttc tcgaaagaaa gctcagcaaa ttggacaatc aggtacgctt cattctggct gtggtagagg agcgcctcgt cgttaacaag aggaagaaag cggagctgct ggcgcagttg gagaaggaag gctacgataa gttctccaat gatgatgaat tggggactaa tgcctggaag agacggaaga aacaagccaa gaagaaggag cgccccgagg atgatgaaga ggatgaggaa gaagaaaaga agaataaaaa gaaagataag aagaagacag agaatgggta tgattacctg ctttcgatga agttatggaa cttgacaatg gagaaggtgg aggagttaat tgctcagcga gacaaaaaga aagaggaact ggaagagctg aaacgaacgg gcgtcacaac tctttgggag cgtgacctcc aagctctcat ggaaagctac gaggcatttg agcaagcttg ggaagaggaa gaagaggaag agctgggtga tggctcaaag aataatcgca gaaggcgaaa accagccgct gctcctcgca aacgcgccgt cagtgccaca aagaaagcga aaggcaaaga cttgatggat gacgaggaag acgatgatga ctttaccgat gattcatgga agtacaatga aaacgtcgca ccaaacaaaa agacaacaac aactgcagca gcgaaagcga ccacaaccac aacaagaaag aaacaaggta cactggatgg agtgctacag aaaaagacaa cagcaacgac gacaacagct acaaagaagg caacgatagg acgtgccgcc gctgctgcaa agaagaccat ctcgttggtt gatgagaaag atgaagatga agatgatgat gatatggagg aagagaagaa agtaacaggg atgacatcga agaaacgaca attagctcca aagcaagctg catcatctac agtgaagatg gaaatagcag atgaggaaga agaagaagcg gaagacaaag atgaagatga agagtttctt tctttggcgg atcgtattct gaagaagaag tccggtgggg cagcatcaac aaagacaaca acaaccaaga aaaaggcttc tgtgtttgat agtctgagcg atttggattc tagcgatgac gaagacagca aacccgttgt cgttgctccc aaacgtggcg ctgctgctgc aaagaaacaa gccacgacat ccaagacaac cgcaaagacc acagtagcaa gacctgcgaa acgaaaggcc gctgcaacca acgatgctga tgatggcgag tcttcttcat caacatcaac aactgcggga agaggtgcaa agaaggcaaa gattactgat tttatgcaat ccgctgcaag caagaggaaa gctgctccgc ctaagaaaga agaaattgag gaagaggaag aagaagatga tgaagaggaa gaagaagaag aagaagagga agaaaagcct caggctccaa aagcccaagc tcctccttca cgaaggctga aaagagcgga tagccaactg aagctcgcaa aaggaccttc cggaccggtc tctctaactc gaggtcttag catctccgac ttttatacca actatgccgg tatgcatgac cagatccacg ctgctccaag ggacagcttc tccaacccgc aaggaagaga gtacgatctt ggccatgaag gagcctttgg acagttctca gtgcttgttg gtctcttttg tcctgaccag ctcagcgagg aaactttcat gaaggatgct ggaaaggcat tgggtgagaa gggcttccga gtttatgtga cgaccagcga agagggcttt gcaaagaact tgccggccaa tgacatcggc ttcgtcatta gcacacgcac cgtatcggaa catactcctg cggtggcgga ggcctgttgg cgccaccacc agtccggtag aggtctcgcc gtttgggcgg agaacgcccc ttactttgct accgctaatg aagtcctaaa gaagctttcg cctggcactg ttattacggg atacgattcg ggcggtaaat cactgtcagt tggagatgcg aacaagaggc aacaatttgg aaggcacttg ttgacatcgg gcattaccaa cctcttcgag ggtatcacca tcagctatcc agagtccata ggcgagaatc tgaagatcct tgccacttcc tccgacaacc acccatgcat gctctgtgcc gattatccgg acatcaaaga tcatgaaggg cgcatcgtgg tggattgtgg cttcaccaaa ctgttctgcc attgggatac agcaggcact gctcgttatg tccgcaacgt gtgtgtatgg ctgttgggat tggagcacag gctgaaactg ggtatgcctc tgcaaggccc gatgaaactt gacacagacg gaggatcatc ctcttcatca tcttcttctt cctcagcaac ttccaaagct gagtaa
Details for BamaA.19955.a.B2.GE43481
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 46kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHME EDTEAWMAYQ RMSPHDHILI RPDTYVGSVE RTEATMWVYD DGQGMTLRPI SYVPGLYKIF DEILVNAADN YQRDKSMSEI RVTIDAEKNT IEVWNDGEGI PVEMHKKEDM YVPELIFGHL LTSSNYDDEK EKVTGGRNGY GAKLANIFST KFSIETVDSK RKKKFYMEWT DNMRTKGKQK ITTISASKDY TKVTFTPDLE KFGMTEFDED TVSLLTKRVY DVAGCNSKLK VKLNGKLLKI SSFEKYVDLY FQGEKLPHIF EKNDRWEVCV SVSKDSEFRQ VSFVNSICTT QGGSHVKYIV DKLVKQLIPA IKKKAKVSTI KPAQVKAHLW VFINCLIVNP AFNSQTKDTL TSQPKSFGST CDLSENFIKQ VVNKTAIATY TGEFAKMKEN
Validated NT Sequence
ggttttcctt catcttcgca aactctcccg tgtatgtcgc aattgcagtc ttgtttacca cttgcttgat aaagttctca gagaggtcac aggttgaccc aaagctcttt ggttgcgagg tcaacgtgtc cttcgtttgg gagttgaacg caggattgac aatgaggcaa ttgataaaca cccacaagtg cgccttaacc tgagcgggct taatggtaga gaccttggct ttcttcttga tggccggaat gagttgcttt accaacttgt ccacaatgta tttcacgtgg ctgcctcctt gagtggtgca aatgctgttg acaaagctaa cttgacgaaa ctcggagtct ttgctcacgc tcacgcagac ctcccatcga tcgtttttct caaagatatg agggagcttc tctccttgaa agtacaaatc tacatatttc tcaaatgaac tgatcttcaa aagctttcca ttgagcttga ctttgagctt ggagttgcag ccagcaacat cgtacacacg cttggtaagg agactaaccg tatcttcatc aaactcggtc atcccaaact tctcaagatc gggcgtgaaa gtgaccttgg tgtagtcttt ggaggcggaa atggtagtga ttttctgctt tcctttcgtc ctcatgttat cagtccattc catgtagaac tttttctttc gctttgagtc caccgtctcg atggaaaact tggtggagaa gatgttggcc agcttcgcac cataaccgtt tcttccacca gttacctttt ctttttcgtc gtcgtagttc gaagaggtca agaggtgacc gaaaataagc tctggaacat acatgtcctc ttttttgtgc atctccacag gaataccctc accgtcgttc caaacttcga tggtgttttt ctccgcgtcg atggtaactc ttatctcgga catgctcttg tctctttggt agttgtccgc cgcgttcacc aggatctcgt caaagatctt gtagagacca ggaacatagg aaatgggacg caaggtcatg ccctgtccat catcatagac ccacatcgtg gcttccgttc tctccacaga gcccacgtag gtgtcaggac gaatgaggat gtggtcatga ggtgacatgc gttggtaggc catccatgct tcggtgtctt cctccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHEE DTEAWMAYQR MSPHDHILIR PDTYVGSVER TEATMWVYDD GQGMTLRPIS YVPGLYKIFD EILVNAADNY QRDKSMSEIR VTINAENNTI EVWNDGEGIP VEMHKKEDMY VPELIFGHLL TSSNYDDEKE KVTGGRNGYG AKLANIFSTK FSIETVDSKR KKKFYMEWTD NMRTKGKQKI TTISASKDYT KVTFTPDLEK FGMTEFDEDT VSLLTKRVYD VAGCNSKLKV KLNGKLLKIS SFEKYVDLYF QGEKLPHIFE KNDRWEVCVS VSKDSEFRQV SFVNSICTTQ GGSHVKYIVD KLVKQLIPAI KKKAKVSTIK PAQVKAHLWV FINCLIVNPA FNSQTKDTLT SQPKSFGSTC DLSENFIKQV VNKTAIATYT GEFAKMKENQ ELQKKG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaggaag acaccgaagc atggatggcc taccaacgca tgtcacctca tgaccacatc ctcattcgtc ctgacaccta cgtgggctct gtggagagaa cggaagccac gatgtgggtc tatgatgatg gacagggcat gaccttgcgt cccatttcct atgttcctgg tctctacaag atctttgacg agatcctggt gaacgcggcg gacaactacc aaagagacaa gagcatgtcc gagatacgag ttaccatcaa cgcggagaac aacaccatcg aagtttggaa cgacggtgag ggtattcctg tggagatgca caaaaaagag gacatgtatg ttccagagct tattttcggt cacctcttga cctcttcgaa ctacgacgac gaaaaagaaa aggtaactgg tggaagaaac ggttatggcg cgaagctggc caacatcttc tccaccaagt tttccatcga gacggtggac tcaaagcgaa agaaaaagtt ctacatggaa tggactgata acatgaggac gaaaggaaag cagaaaatca ctaccatttc cgcctccaaa gactacacca aggtcacttt cacgcccgat cttgagaagt ttgggatgac cgagtttgat gaagatacgg ttagtctcct taccaagcgt gtgtacgatg ttgctggctg caactccaag ctcaaagtca agctcaatgg aaagcttttg aagatcagtt catttgagaa atatgtagat ttgtactttc aaggagagaa gctccctcat atctttgaga aaaacgatcg atgggaggtc tgcgtgagcg tgagcaaaga ctccgagttt cgtcaagtta gctttgtcaa cagcatttgc accactcaag gaggcagcca cgtgaaatac attgtggaca agttggtaaa gcaactcatt ccggccatca agaagaaagc caaggtctct accattaagc ccgctcaggt taaggcgcat ttgtgggtgt ttatcaattg cctcattgtc aatcctgcgt tcaactccca aacgaaggac acgttgacct cgcaaccaaa gagctttggg tcaacctgtg acctctctga gaactttatc aagcaagtgg taaacaagac tgcaattgcg acatacacgg gagagtttgc gaagatgaag gaaaaccaag aactgcaaaa gaaaggctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BamaA.19955.a.B2.PW38787
PURIFICATION DATe: 1/17/2020
CONCENTRATION: 16.4mg/ml
OBSERVED MW: 46kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME EDTEAWMAYQ RMSPHDHILI RPDTYVGSVE RTEATMWVYD DGQGMTLRPI SYVPGLYKIF DEILVNAADN YQRDKSMSEI RVTIDAEKNT IEVWNDGEGI PVEMHKKEDM YVPELIFGHL LTSSNYDDEK EKVTGGRNGY GAKLANIFST KFSIETVDSK RKKKFYMEWT DNMRTKGKQK ITTISASKDY TKVTFTPDLE KFGMTEFDED TVSLLTKRVY DVAGCNSKLK VKLNGKLLKI SSFEKYVDLY FQGEKLPHIF EKNDRWEVCV SVSKDSEFRQ VSFVNSICTT QGGSHVKYIV DKLVKQLIPA IKKKAKVSTI KPAQVKAHLW VFINCLIVNP AFNSQTKDTL TSQPKSFGST CDLSENFIKQ VVNKTAIATY TGEFAKMKEN
Validated NT Sequence
ggttttcctt catcttcgca aactctcccg tgtatgtcgc aattgcagtc ttgtttacca cttgcttgat aaagttctca gagaggtcac aggttgaccc aaagctcttt ggttgcgagg tcaacgtgtc cttcgtttgg gagttgaacg caggattgac aatgaggcaa ttgataaaca cccacaagtg cgccttaacc tgagcgggct taatggtaga gaccttggct ttcttcttga tggccggaat gagttgcttt accaacttgt ccacaatgta tttcacgtgg ctgcctcctt gagtggtgca aatgctgttg acaaagctaa cttgacgaaa ctcggagtct ttgctcacgc tcacgcagac ctcccatcga tcgtttttct caaagatatg agggagcttc tctccttgaa agtacaaatc tacatatttc tcaaatgaac tgatcttcaa aagctttcca ttgagcttga ctttgagctt ggagttgcag ccagcaacat cgtacacacg cttggtaagg agactaaccg tatcttcatc aaactcggtc atcccaaact tctcaagatc gggcgtgaaa gtgaccttgg tgtagtcttt ggaggcggaa atggtagtga ttttctgctt tcctttcgtc ctcatgttat cagtccattc catgtagaac tttttctttc gctttgagtc caccgtctcg atggaaaact tggtggagaa gatgttggcc agcttcgcac cataaccgtt tcttccacca gttacctttt ctttttcgtc gtcgtagttc gaagaggtca agaggtgacc gaaaataagc tctggaacat acatgtcctc ttttttgtgc atctccacag gaataccctc accgtcgttc caaacttcga tggtgttttt ctccgcgtcg atggtaactc ttatctcgga catgctcttg tctctttggt agttgtccgc cgcgttcacc aggatctcgt caaagatctt gtagagacca ggaacatagg aaatgggacg caaggtcatg ccctgtccat catcatagac ccacatcgtg gcttccgttc tctccacaga gcccacgtag gtgtcaggac gaatgaggat gtggtcatga ggtgacatgc gttggtaggc catccatgct tcggtgtctt cctccatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHEE DTEAWMAYQR MSPHDHILIR PDTYVGSVER TEATMWVYDD GQGMTLRPIS YVPGLYKIFD EILVNAADNY QRDKSMSEIR VTINAENNTI EVWNDGEGIP VEMHKKEDMY VPELIFGHLL TSSNYDDEKE KVTGGRNGYG AKLANIFSTK FSIETVDSKR KKKFYMEWTD NMRTKGKQKI TTISASKDYT KVTFTPDLEK FGMTEFDEDT VSLLTKRVYD VAGCNSKLKV KLNGKLLKIS SFEKYVDLYF QGEKLPHIFE KNDRWEVCVS VSKDSEFRQV SFVNSICTTQ GGSHVKYIVD KLVKQLIPAI KKKAKVSTIK PAQVKAHLWV FINCLIVNPA FNSQTKDTLT SQPKSFGSTC DLSENFIKQV VNKTAIATYT GEFAKMKENQ ELQKKG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGAGGAAG ACACCGAAGC ATGGATGGCC TACCAACGCA TGTCACCTCA TGACCACATC CTCATTCGTC CTGACACCTA CGTGGGCTCT GTGGAGAGAA CGGAAGCCAC GATGTGGGTC TATGATGATG GACAGGGCAT GACCTTGCGT CCCATTTCCT ATGTTCCTGG TCTCTACAAG ATCTTTGACG AGATCCTGGT GAACGCGGCG GACAACTACC AAAGAGACAA GAGCATGTCC GAGATACGAG TTACCATCAA CGCGGAGAAC AACACCATCG AAGTTTGGAA CGACGGTGAG GGTATTCCTG TGGAGATGCA CAAAAAAGAG GACATGTATG TTCCAGAGCT TATTTTCGGT CACCTCTTGA CCTCTTCGAA CTACGACGAC GAAAAAGAAA AGGTAACTGG TGGAAGAAAC GGTTATGGCG CGAAGCTGGC CAACATCTTC TCCACCAAGT TTTCCATCGA GACGGTGGAC TCAAAGCGAA AGAAAAAGTT CTACATGGAA TGGACTGATA ACATGAGGAC GAAAGGAAAG CAGAAAATCA CTACCATTTC CGCCTCCAAA GACTACACCA AGGTCACTTT CACGCCCGAT CTTGAGAAGT TTGGGATGAC CGAGTTTGAT GAAGATACGG TTAGTCTCCT TACCAAGCGT GTGTACGATG TTGCTGGCTG CAACTCCAAG CTCAAAGTCA AGCTCAATGG AAAGCTTTTG AAGATCAGTT CATTTGAGAA ATATGTAGAT TTGTACTTTC AAGGAGAGAA GCTCCCTCAT ATCTTTGAGA AAAACGATCG ATGGGAGGTC TGCGTGAGCG TGAGCAAAGA CTCCGAGTTT CGTCAAGTTA GCTTTGTCAA CAGCATTTGC ACCACTCAAG GAGGCAGCCA CGTGAAATAC ATTGTGGACA AGTTGGTAAA GCAACTCATT CCGGCCATCA AGAAGAAAGC CAAGGTCTCT ACCATTAAGC CCGCTCAGGT TAAGGCGCAT TTGTGGGTGT TTATCAATTG CCTCATTGTC AATCCTGCGT TCAACTCCCA AACGAAGGAC ACGTTGACCT CGCAACCAAA GAGCTTTGGG TCAACCTGTG ACCTCTCTGA GAACTTTATC AAGCAAGTGG TAAACAAGAC TGCAATTGCG ACATACACGG GAGAGTTTGC GAAGATGAAG GAAAACCAAG AACTGCAAAA GAAAGGCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT