BamaA.19900.a
Glucokinase
| CENTER ID: | BamaA.19900.a |
| ORGANISM: | Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BamaA.19900.a.B2.GE42938 | N-term truncation | 20 | 377 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6VZZ
DEPOSITED: 2/28/2020
DETERMINATION: XRay
CLONE: BamaA.19900.a.MP3.GE43310
PROTEIN: BamaA.19900.a.MP3.PS38592
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_10691 |
Publications by SSGCID
Characterization of glucokinases from pathogenic free-living amoebae
Abendroth JA, Phan IQ, Milanes JE, Suryadi J, Khalifa MM, Golden JE, Morris JC, Rozema S, Rice CA, Zigweid R, Fenwick MK, Monaghan NP, Harding EM, Morris CS, Wilson S
Antimicrob. Agents Chemother. - 2022
PMID: 35604214; PMCID: PMC9211422
PDB:
6VZZ
External Resources
| RESOURCE | REFERENCE ID |
|---|
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MELSQEVTET LKQWRVAAEG LKYFVGVDIG GTNTRLALAR AEDHDTFLQV LKVKADDTRK LIAFFDSVSD SIKDLLGVEA SGACLAGAGR ISPDGEVLDV TNFHGDATHH RLKRSELPSF LFPPTKTHFI NDLEGTCYGV SSMNASGALD QCFKPLWGPA SSDKVTLQPA HYLVLAVGTG LGIATLLSLR RSPVRGGFQV MPMEFGHVAI SPVGPANVAY AEETRLLEYI SEKLYNKEHA IEYEDIVSGR GLAITYKWVL DEANVQDESL RSLDAGKIAK NATNSEACPY AHKAMMLHFK FLMRIAKNLC IGLQVRGMLL AGDNQVNNNL FLEQNLESLR AEFFDHPKKA WIEDIDLFTQ TKPVNLNLHG ALYVARG
NT Sequence
atggaactga gccaagaagt tacagagact ttgaaacagt ggcgggtcgc tgctgagggc ctcaaatatt tcgtgggagt tgacattggt ggaaccaaca ctcgtcttgc tctggctcgt gcagaggacc acgacacctt cctccaagtc ctcaaagtga aggctgacga cacgaggaag ctcatcgctt tctttgatag cgtaagtgac agtatcaaag atctattggg agtggaggcc tctggagctt gcttggccgg tgctggtagg atctctcctg acggtgaagt gctggacgtg acaaacttcc acggagatgc cacgcaccac aggctgaagc ggtcggagct gccaagcttc ctgttcccac ccaccaaaac gcactttatc aatgacttgg agggaacatg ttatggcgta tcatctatga acgccagtgg tgctttggat caatgcttta aaccattatg gggacctgct tcttctgata aggtcaccct gcagcctgct cactatctgg tgttagctgt aggtactggg ttgggcatag ccactctcct ttcgctaagg agaagtcctg tcagaggtgg gtttcaagta atgccaatgg agtttggtca tgtcgccatt tctccagttg gccctgctaa tgtcgcctac gctgaggaaa cacggttgtt ggagtatatc agcgaaaaac tgtacaacaa agagcatgca attgagtacg aggatattgt gagtggaaga ggccttgcca taacgtacaa gtgggtactc gatgaagcta atgtacagga tgaatctctg agaagcttgg atgctggaaa gatcgccaag aatgctacaa acagtgaggc ctgcccgtat gcccataagg ccatgatgct tcacttcaag ttcctgatgc gtattgccaa gaacctctgc attggtttgc aagtgcgagg tatgctgctt gctggagaca atcaggtgaa caacaactta ttcttggagc aaaacttgga gtccctgcgg gcagaattct tcgaccatcc aaagaaagct tggatagagg acattgacct gttcacccaa accaaacccg tcaatctgaa cctacatgga gccttgtatg ttgcgcgagg atag
Details for BamaA.19900.a.B2.GE42938
| HARVESTED ON: | 3/4/2019 |
| SEQUENCED ON: | 2/5/2020 |
| EXPECTED MW: | 40kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG LKYFVGVDIG GTNTRLALAR AEDHDTFLQV LKVKADDTRK LIAFFDSVSD SIRDLLGVEA SGACLAGAGR ISPDGEVLDV TNFHGDATHR RLKRSELPSF LFPPTKTHFI NDLEGTCYGV SSMNASGALD QCFKPLWGPA SSDKVTLQPD HYLVLAVGTG LGIATLLSLR RSPVRGGFQV MPMEFGHVAI SPVGPANAAY AEETRLLEYI SEKLYNKEHA IEYEDIVSGR GLAITYKWVL DEANVQDESL RSLDAGKIAK NATNSEACPY AHKAMMLHFK FLMRIAKNLC IGLQVRGMLL AGDNQVNNNL FLEQNLESLR AEFFDHPKKA WIEDIDLFTQ TKPVNLNLHG ALYVARG
Validated NT Sequence
atggctcacc accaccacca ccatatgggc ctcaaatatt tcgtgggagt tgacattggt ggaaccaaca ctcggcttgc tctggctcgc gcagaggacc acgacacctt cctccaagtc ctcaaagtga aggctgacga cacgaggaag ctcatcgctt tctttgatag cgtaagtgac agtatcagag atctattggg ggtggaggcc tctggagctt gcttggccgg tgctggtagg atctctcctg acggtgaagt gctggacgtg acaaacttcc acggagatgc cacacaccgc aggctgaagc ggtcggagct g-ccaagctt cctgttccca ccc-accaaa acgcacttta tcaatgactt ggagggaacc tgttatggcg tatcatctat gaacgccagt ggtgctttgg atcaatgctt taaaccatta tgggggcctg cttcttctga taaggtcacc ctgcagcctg atcactatct ggtgttggct gtaggtactg ggttgggtat agccactctc ctttcgctaa ggagaagtcc tgtcagaggt gggtttcaag taatgccaat ggagtttggt catgtcgcca tttctccagt tggccctgct aatgccgcct acgctgagga aacacggttg ttggagtata tcagcgaaaa actgtataac aaagagcatg caattgagta cgaggacatt gtgagtggaa gaggccttgc gataacatac aagtgggtac tcgatgaagc taacgtacaa gatgaatctc tgagaagctt ggatgctgga aagatcgcca agaatgctac aaacagtgag gcttgcccgt atgcccataa ggcaatgatg cttcacttca agttcctgat gcgtattgcc aagaacctct gcattggttt gcaagtgcga ggtatgctgc ttgctggaga caatcaggtg aacaacaact tattcttgga gcaaaacttg gagtccctgc gggcagaatt cttcgaccat ccaaagaaag cttggattga ggacattgac ctgttcaccc aaaccaaacc cgtcaatctg aacctacatg gagccttgta tgttgcgcga ggataa
Expected Protein Sequence
MAHHHHHHGL KYFVGVDIGG TNTRLALARA EDHDTFLQVL KVKADDTRKL IAFFDSVSDS IKDLLGVEAS GACLAGAGRI SPDGEVLDVT NFHGDATHHR LKRSELPSFL FPPTKTHFIN DLEGTCYGVS SMNASGALDQ CFKPLWGPAS SDKVTLQPAH YLVLAVGTGL GIATLLSLRR SPVRGGFQVM PMEFGHVAIS PVGPANVAYA EETRLLEYIS EKLYNKEHAI EYEDIVSGRG LAITYKWVLD EANVQDESLR SLDAGKIAKN ATNSEACPYA HKAMMLHFKF LMRIAKNLCI GLQVRGMLLA GDNQVNNNLF LEQNLESLRA EFFDHPKKAW IEDIDLFTQT KPVNLNLHGA LYVARG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcctca aatatttcgt gggagttgac attggtggaa ccaacactcg tcttgctctg gctcgtgcag aggaccacga caccttcctc caagtcctca aagtgaaggc tgacgacacg aggaagctca tcgctttctt tgatagcgta agtgacagta tcaaagatct attgggagtg gaggcctctg gagcttgctt ggccggtgct ggtaggatct ctcctgacgg tgaagtgctg gacgtgacaa acttccacgg agatgccacg caccacaggc tgaagcggtc ggagctgcca agcttcctgt tcccacccac caaaacgcac tttatcaatg acttggaggg aacatgttat ggcgtatcat ctatgaacgc cagtggtgct ttggatcaat gctttaaacc attatgggga cctgcttctt ctgataaggt caccctgcag cctgctcact atctggtgtt agctgtaggt actgggttgg gcatagccac tctcctttcg ctaaggagaa gtcctgtcag aggtgggttt caagtaatgc caatggagtt tggtcatgtc gccatttctc cagttggccc tgctaatgtc gcctacgctg aggaaacacg gttgttggag tatatcagcg aaaaactgta caacaaagag catgcaattg agtacgagga tattgtgagt ggaagaggcc ttgccataac gtacaagtgg gtactcgatg aagctaatgt acaggatgaa tctctgagaa gcttggatgc tggaaagatc gccaagaatg ctacaaacag tgaggcctgc ccgtatgccc ataaggccat gatgcttcac ttcaagttcc tgatgcgtat tgccaagaac ctctgcattg gtttgcaagt gcgaggtatg ctgcttgctg gagacaatca ggtgaacaac aacttattct tggagcaaaa cttggagtcc ctgcgggcag aattcttcga ccatccaaag aaagcttgga tagaggacat tgacctgttc acccaaacca aacccgtcaa tctgaaccta catggagcct tgtatgttgc gcgaggatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at