BamaA.19525.a

3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) (HMGR)

CENTER ID: BamaA.19525.a
ORGANISM: Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamaA.19525.a.B2.GE43480 substrate-binding and catalytic domains 179 600
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamaA.19525.a.B2.PW38792 substrate-binding and catalytic domains 179 600

External Resources

RESOURCE REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGQELTPLY QWGFSLRTPK FLVPLLNNNL FAFGKEAVSS GLPLPDDQDE ALAHHHPDND GKLPHQLPPP ILQKRKKKKA LPASASASPP CSSSLSSSSS NSTDNNIFPT EQLSSSSSSS QVFVSSSSSA TTTLSLSQPS PSSSPSCSSS SPQQTTTTTT ITASSSTSSS SSSSSSPSQP LSERSDEDIL QMLKEGRIAA HQLEHRLQDL PRAVHLRRLL LASQLESQQR AEVSLSSLPT ADMDYAAVQG RCCENVIGYV CVPVGVAGPL LLNGQQVYLP MATTEGCLVA STHRGCKAIT ASGGATAVVV GDGMTRGPCL KLPSAAAAAE LKEWLERAEN RAQVETAFNS TSRFARLIDI KTAIAGRNLY LRFKATTGDA MGMNMISKGT NEALEVILAQ FPEAHLVSLS GNYCTDKKPS AINWVEGRGK SVVCEAVIKG EIVETVLKTT VEAMVQVNID KNLVGSAMAG SVGGFNAHAA NIVTAMFIAT GQDPAQNVES SNCITLIEPT NGGKDLYMSV TMPSLEVGTV GGGTHLGAQA ACLDILNVKG ASQEKPGDNA RQLARVICGG VLAGELSLMA ALAANHLTRS HMQLNRGNKL
NT Sequence
atgtctggac aagaacttac tccgttgtac caatggggtt ttagtttgcg aacgccaaag tttttggtgc cgctgttgaa caacaacctc tttgctttcg gcaaggaggc cgtcagcagc gggcttcctc tgcccgacga ccaggacgag gctttggccc accaccaccc tgacaacgac ggcaaattac cacatcagct gcctccgccc atcctacaaa agagaaagaa gaaaaaggct cttcctgctt ctgcttctgc ttctcctcct tgttcttcct ctctttcttc ttcttcttcc aactccacag acaacaacat ttttcctact gagcaactct cctcttcttc ttcgtcttcc caagtttttg tgtcttcttc gtcttctgca acaacaacac tctccttatc acaaccttct ccttcttctt caccttcttg ttcttcttcc tcacctcaac aaaccacaac cactacgacg ataactgctt cctcttctac ctcttcttcc tcctcttctt cctcttctcc ttcacaaccc ctttcggaac gttccgatga agacattttg caaatgttga aggagggccg catagctgcc caccagctcg aacaccgact gcaagacctc cctcgtgctg ttcacctccg tcggcttctt cttgcaagcc aattagagag ccaacaacgc gcagaggtaa gcctatcctc cttaccaacc gcagatatgg actatgcggc cgtgcaaggc cgctgctgcg agaacgtgat aggctacgtg tgcgttcctg tgggcgtggc ggggcctctc ctcctcaacg ggcaacaggt gtacctgccg atggccacca cggaggggtg cctggtggct tccacgcacc gaggctgtaa ggccataacc gcgtcgggag gagccactgc agttgtggtt ggggatggaa tgacgcgtgg gccttgcttg aaactgccgt cggcggcggc ggctgctgag ctaaaggagt ggcttgaaag ggcggagaat agagctcaag tggagactgc ctttaattca accagtcggt ttgctcgtct aattgatata aaaacagcaa tagctggaag gaacctatac cttaggttca aggccacaac aggggacgcc atgggcatga atatgatttc caaaggcacc aacgaggccc tcgaggtgat cctcgcccag ttccccgagg cccatcttgt cagcctctct ggcaactact gcaccgacaa gaagccctcc gccatcaact gggttgaggg cagaggcaag tcggttgtct gtgaggctgt tatcaaggga gagattgtgg agacggtact caagaccacg gtggaggcca tggtccaagt caacatagat aaaaacctgg tgggctcagc tatggcgggt tctgttgggg gtttcaacgc gcatgccgct aatattgtga cggctatgtt catagcgaca gggcaggatc ctgcccagaa cgtggagagc tcgaattgca tcactcttat tgaaccaacg aatggaggca aggatttata tatgtcagtg acaatgcctt cattggaggt gggcacagtg ggaggaggca ctcatttggg agcacaggca gcgtgcctcg acatactcaa cgtgaaggga gcatcgcaag aaaaacctgg tgataacgca aggcagctgg cacgagtcat ctgcggtggt gtgcttgctg gggaactttc gttgatggca gctcttgcag cgaatcattt gacacgaagt cacatgcagc tcaatcgagg aaacaagttg tga
Details for BamaA.19525.a.B2.GE43480
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 46kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ PLSERSDEDI LQMLKEGRIA AHQLEHQLQD LPRAVHLRRL LLASQLESQQ RAEVSLSSLP TADMDYAAVQ GRCCENVIGY VRVPVGVAGP LLLNGQQVYL PMATTEGCLV ASTHRGCKAI TASGGATAVV VGDGMTRGPC LKLPSAAAAA ELKEWLERAE NRAQVEAAFN STSRFARLID IKTAIAGRNL YLRFKATTGD AMGMNMISKG TNEALEVILA QFPEAHLVSL SGNYCTDKKP SAINWVEGRG KSVVCEAVIK GEIVETVLKT TVEAMVQVNI DKNLVGSAMA GSVGGFNAHA ANIVTAMFIA TGQDPAQNVE SSNCITLIEP TNGGKDLYMS VTMPSLEVGT VGGGTHLGAQ AACLDILNVK GASQEKPGDN ARQLARVICG GVLAGELSLM AALAANHLTR SHMQLNRGNK L
Validated NT Sequence
tgcatgtgac ttcgtgtcaa atgattcgct gcaagagctg ccatcaacga aagttcccca gcaagcacac caccgcagat gactcgtgcc agctgtcttg cgttatcacc aggtttttct tgcgatgctc ccttcacgtt gagtatgtcg aggcacgctg cctgtgctcc caaatgagtg cctcctccca ctgtgcccac ctccaatgaa ggcattgtca ctgacatata taaatccttg cctccattcg ttggttcaat aagagtgatg caattcgagc tctccacgtt ctgagcagga tcctgccctg ttgcaatgaa catagccgtc acaatattgg cggcatgcgc gttgaaaccc ccaacagaac ccgccatagc tgagcccacc aggtttttat ctatgttgac ttggaccatg gcctccaccg tggtcttgag caccgtctcc acaatctctc ccttgataac agcctcacag acaaccgact tgcctctgcc ctcaacccag ttgatggcgg agggcttctt gtcggtgcag tagttgccag agaggctgac aagatgggcc tcggggaact gggcgaggat cacctcgagg gcctcgttgg tgcctttgga aatcatattc atgcccatgg cgtcccctgt tgtggccttg aacctaaggt ataggtttct tccagctatt gctgttttta tatcaattag acgagcaaac cgactggttg aattaaaggc agcctccact tgagctctat tctccgccct ttcaagccac tccttcagct cagcagcagc cgcagccgac ggcagtttca agcaaggccc acgcgtcatt ccatccccaa ccacaactgc agtggctcct cccgacgcgg ttatggcctt acagcctcgg tgcgtggaag ccaccaggca cccctccgtg gtggccatcg gcaggtacac ctgttgcccg ttgagaagga gaggccccgc cacgcccaca ggaacgcgca cgtagcctat cacgttctcg cagcagcggc cttgcacggc cgcatagtcc atatctgcgg ttggtaagga ggataggctt acctctgcgc gttgttggct ctctaattgg cttgcaagaa gaagccgacg gaggtgaaca gcacgaggga ggtcttgcag ttggtgttct agttggtggg cagctatgcg gccctccttc aacatttgca aaatgtcttc atcggaacgt tccgaaaggg gttgcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHQP LSERSDEDIL QMLKEGRIAA HQLEHRLQDL PRAVHLRRLL LASQLESQQR AEVSLSSLPT ADMDYAAVQG RCCENVIGYV CVPVGVAGPL LLNGQQVYLP MATTEGCLVA STHRGCKAIT ASGGATAVVV GDGMTRGPCL KLPSAAAAAE LKEWLERAEN RAQVETAFNS TSRFARLIDI KTAIAGRNLY LRFKATTGDA MGMNMISKGT NEALEVILAQ FPEAHLVSLS GNYCTDKKPS AINWVEGRGK SVVCEAVIKG EIVETVLKTT VEAMVQVNID KNLVGSAMAG SVGGFNAHAA NIVTAMFIAT GQDPAQNVES SNCITLIEPT NGGKDLYMSV TMPSLEVGTV GGGTHLGAQA ACLDILNVKG ASQEKPGDNA RQLARVICGG VLAGELSLMA ALAANHLTRS HMQLNRGNKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaacccc tttcggaacg ttccgatgaa gacattttgc aaatgttgaa ggagggccgc atagctgccc accagctcga acaccgactg caagacctcc ctcgtgctgt tcacctccgt cggcttcttc ttgcaagcca attagagagc caacaacgcg cagaggtaag cctatcctcc ttaccaaccg cagatatgga ctatgcggcc gtgcaaggcc gctgctgcga gaacgtgata ggctacgtgt gcgttcctgt gggcgtggcg gggcctctcc tcctcaacgg gcaacaggtg tacctgccga tggccaccac ggaggggtgc ctggtggctt ccacgcaccg aggctgtaag gccataaccg cgtcgggagg agccactgca gttgtggttg gggatggaat gacgcgtggg ccttgcttga aactgccgtc ggcggcggcg gctgctgagc taaaggagtg gcttgaaagg gcggagaata gagctcaagt ggagactgcc tttaattcaa ccagtcggtt tgctcgtcta attgatataa aaacagcaat agctggaagg aacctatacc ttaggttcaa ggccacaaca ggggacgcca tgggcatgaa tatgatttcc aaaggcacca acgaggccct cgaggtgatc ctcgcccagt tccccgaggc ccatcttgtc agcctctctg gcaactactg caccgacaag aagccctccg ccatcaactg ggttgagggc agaggcaagt cggttgtctg tgaggctgtt atcaagggag agattgtgga gacggtactc aagaccacgg tggaggccat ggtccaagtc aacatagata aaaacctggt gggctcagct atggcgggtt ctgttggggg tttcaacgcg catgccgcta atattgtgac ggctatgttc atagcgacag ggcaggatcc tgcccagaac gtggagagct cgaattgcat cactcttatt gaaccaacga atggaggcaa ggatttatat atgtcagtga caatgccttc attggaggtg ggcacagtgg gaggaggcac tcatttggga gcacaggcag cgtgcctcga catactcaac gtgaagggag catcgcaaga aaaacctggt gataacgcaa ggcagctggc acgagtcatc tgcggtggtg tgcttgctgg ggaactttcg ttgatggcag ctcttgcagc gaatcatttg acacgaagtc acatgcagct caatcgagga aacaagttgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BamaA.19525.a.B2.PW38792
PURIFICATION DATe: 1/27/2020
CONCENTRATION: 37mg/ml
OBSERVED MW: 46kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ PLSERSDEDI LQMLKEGRIA AHQLEHQLQD LPRAVHLRRL LLASQLESQQ RAEVSLSSLP TADMDYAAVQ GRCCENVIGY VRVPVGVAGP LLLNGQQVYL PMATTEGCLV ASTHRGCKAI TASGGATAVV VGDGMTRGPC LKLPSAAAAA ELKEWLERAE NRAQVEAAFN STSRFARLID IKTAIAGRNL YLRFKATTGD AMGMNMISKG TNEALEVILA QFPEAHLVSL SGNYCTDKKP SAINWVEGRG KSVVCEAVIK GEIVETVLKT TVEAMVQVNI DKNLVGSAMA GSVGGFNAHA ANIVTAMFIA TGQDPAQNVE SSNCITLIEP TNGGKDLYMS VTMPSLEVGT VGGGTHLGAQ AACLDILNVK GASQEKPGDN ARQLARVICG GVLAGELSLM AALAANHLTR SHMQLNRGNK L
Validated NT Sequence
tgcatgtgac ttcgtgtcaa atgattcgct gcaagagctg ccatcaacga aagttcccca gcaagcacac caccgcagat gactcgtgcc agctgtcttg cgttatcacc aggtttttct tgcgatgctc ccttcacgtt gagtatgtcg aggcacgctg cctgtgctcc caaatgagtg cctcctccca ctgtgcccac ctccaatgaa ggcattgtca ctgacatata taaatccttg cctccattcg ttggttcaat aagagtgatg caattcgagc tctccacgtt ctgagcagga tcctgccctg ttgcaatgaa catagccgtc acaatattgg cggcatgcgc gttgaaaccc ccaacagaac ccgccatagc tgagcccacc aggtttttat ctatgttgac ttggaccatg gcctccaccg tggtcttgag caccgtctcc acaatctctc ccttgataac agcctcacag acaaccgact tgcctctgcc ctcaacccag ttgatggcgg agggcttctt gtcggtgcag tagttgccag agaggctgac aagatgggcc tcggggaact gggcgaggat cacctcgagg gcctcgttgg tgcctttgga aatcatattc atgcccatgg cgtcccctgt tgtggccttg aacctaaggt ataggtttct tccagctatt gctgttttta tatcaattag acgagcaaac cgactggttg aattaaaggc agcctccact tgagctctat tctccgccct ttcaagccac tccttcagct cagcagcagc cgcagccgac ggcagtttca agcaaggccc acgcgtcatt ccatccccaa ccacaactgc agtggctcct cccgacgcgg ttatggcctt acagcctcgg tgcgtggaag ccaccaggca cccctccgtg gtggccatcg gcaggtacac ctgttgcccg ttgagaagga gaggccccgc cacgcccaca ggaacgcgca cgtagcctat cacgttctcg cagcagcggc cttgcacggc cgcatagtcc atatctgcgg ttggtaagga ggataggctt acctctgcgc gttgttggct ctctaattgg cttgcaagaa gaagccgacg gaggtgaaca gcacgaggga ggtcttgcag ttggtgttct agttggtggg cagctatgcg gccctccttc aacatttgca aaatgtcttc atcggaacgt tccgaaaggg gttgcatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHQP LSERSDEDIL QMLKEGRIAA HQLEHRLQDL PRAVHLRRLL LASQLESQQR AEVSLSSLPT ADMDYAAVQG RCCENVIGYV CVPVGVAGPL LLNGQQVYLP MATTEGCLVA STHRGCKAIT ASGGATAVVV GDGMTRGPCL KLPSAAAAAE LKEWLERAEN RAQVETAFNS TSRFARLIDI KTAIAGRNLY LRFKATTGDA MGMNMISKGT NEALEVILAQ FPEAHLVSLS GNYCTDKKPS AINWVEGRGK SVVCEAVIKG EIVETVLKTT VEAMVQVNID KNLVGSAMAG SVGGFNAHAA NIVTAMFIAT GQDPAQNVES SNCITLIEPT NGGKDLYMSV TMPSLEVGTV GGGTHLGAQA ACLDILNVKG ASQEKPGDNA RQLARVICGG VLAGELSLMA ALAANHLTRS HMQLNRGNKL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCAACCCC TTTCGGAACG TTCCGATGAA GACATTTTGC AAATGTTGAA GGAGGGCCGC ATAGCTGCCC ACCAGCTCGA ACACCGACTG CAAGACCTCC CTCGTGCTGT TCACCTCCGT CGGCTTCTTC TTGCAAGCCA ATTAGAGAGC CAACAACGCG CAGAGGTAAG CCTATCCTCC TTACCAACCG CAGATATGGA CTATGCGGCC GTGCAAGGCC GCTGCTGCGA GAACGTGATA GGCTACGTGT GCGTTCCTGT GGGCGTGGCG GGGCCTCTCC TCCTCAACGG GCAACAGGTG TACCTGCCGA TGGCCACCAC GGAGGGGTGC CTGGTGGCTT CCACGCACCG AGGCTGTAAG GCCATAACCG CGTCGGGAGG AGCCACTGCA GTTGTGGTTG GGGATGGAAT GACGCGTGGG CCTTGCTTGA AACTGCCGTC GGCGGCGGCG GCTGCTGAGC TAAAGGAGTG GCTTGAAAGG GCGGAGAATA GAGCTCAAGT GGAGACTGCC TTTAATTCAA CCAGTCGGTT TGCTCGTCTA ATTGATATAA AAACAGCAAT AGCTGGAAGG AACCTATACC TTAGGTTCAA GGCCACAACA GGGGACGCCA TGGGCATGAA TATGATTTCC AAAGGCACCA ACGAGGCCCT CGAGGTGATC CTCGCCCAGT TCCCCGAGGC CCATCTTGTC AGCCTCTCTG GCAACTACTG CACCGACAAG AAGCCCTCCG CCATCAACTG GGTTGAGGGC AGAGGCAAGT CGGTTGTCTG TGAGGCTGTT ATCAAGGGAG AGATTGTGGA GACGGTACTC AAGACCACGG TGGAGGCCAT GGTCCAAGTC AACATAGATA AAAACCTGGT GGGCTCAGCT ATGGCGGGTT CTGTTGGGGG TTTCAACGCG CATGCCGCTA ATATTGTGAC GGCTATGTTC ATAGCGACAG GGCAGGATCC TGCCCAGAAC GTGGAGAGCT CGAATTGCAT CACTCTTATT GAACCAACGA ATGGAGGCAA GGATTTATAT ATGTCAGTGA CAATGCCTTC ATTGGAGGTG GGCACAGTGG GAGGAGGCAC TCATTTGGGA GCACAGGCAG CGTGCCTCGA CATACTCAAC GTGAAGGGAG CATCGCAAGA AAAACCTGGT GATAACGCAA GGCAGCTGGC ACGAGTCATC TGCGGTGGTG TGCTTGCTGG GGAACTTTCG TTGATGGCAG CTCTTGCAGC GAATCATTTG ACACGAAGTC ACATGCAGCT CAATCGAGGA AACAAGTTGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT