BamaA.18982.a

Sarcoplasmic-endoplasmic reticulum calcium ATPase (ATP2A)

CENTER ID: BamaA.18982.a
ORGANISM: Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamaA.18982.a.B2.GE43479 haloacid dehydrogenase (HAD) domain (cytoplasmic) 364 743
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKEHSIYVR VNSKEATDIN HEDHDSNKSG SGRSSWSLDT AWRLGMEEVV EFMQSDPQAG LTEREAQLRL QQLGPNSLAA KEQESLVIKY LEQFKEPLIL LLLCSCLVSV LIGELADAFG IFIAVCTVNT VGFIQEYRSE KSVEALQNLV AHRTHVLRDG QPREVLAESL VPGDLVVLNV GDRVPADLRL IETINLELDE SILTGETEPS VKCTEQLSSS SSSSSSSSSV PEGGILEGRP RRDTEEFQLA DRSNMAYMGT ITVNGKGKGL VVATSTATEL GRIWEAITSM GEKRTPLQLK MDQLGKQLSL AAFVIVGVIF ILGAIQGKPL LQMFTIGVSL AVAAIPEGLP IVVTVTLALG VTRMANRHAI VRKLPAVEAL GATTAICSDK TGTLTKNEMT VRRLFTTEFI SVSGEGYHHQ FWQQPQGEFF REGCDQPFDP AQDPHLMQLI QVGAICNNAH LREDGVLGQP TEVALLVLAR KAGIEIDLRT LFPRQSEDPF THASKRMSVL CDFPPDARKM TTFHVSSSFS SSSSSQPIYF MKGALPVVLD HCTTISNAQG NIIDLTPQKQ EMLLAAEKEM GENALRVVAV AYGTSSRSMN LVGLCGIMDP PRNGVLEAIR EVQQTGVHVA MVTGDSRETA IAIARELSFF HRDSLALSSA DLQRLSEEEL ASQIERVAVF YRMSPEDKMK IVKAYKNRGH IVAVTGDGVN DAPALKIADI GIAMGKGTDV CREAAEMILV DDNFATIVAA IEEGKSIYNN IKNFLRFQLT TSIATLSIIA SSTLFGLPLP MNPIQILWIN IIMDGPPAQS LGVEPLDKDA MKQPPRDTKQ PVLSKPLLFS TLACALLMLF GTLGIYQSYL ADPSYDIGEG DEEAAAQRTL KASTMAFNTF VFFQMFNAVN CRSDHKSAFQ IKFMNNKFFL LAIGGSLLLQ LAAIYVPILQ MLFETTSLSM HELLQCLMVS SSVFGLDEAR KYFLKAQQEG AVRFNV
NT Sequence
atgaacaagg aacacagtat ctacgtgaga gtcaacagca aagaagccac tgacatcaat catgaggatc acgactccaa caaaagtggc agtggccgtt cctcgtggag cctggacaca gcctggcggt taggcatgga ggaagtggtg gagttcatgc agagcgaccc acaggcgggt ctaacggaac gggaggccca gctccgcctc caacagttgg gtcccaacag cctggcggcc aaggagcagg agtcccttgt catcaagtac ctggagcagt tcaaggagcc gctcattctg ctgctgctct gctcctgcct cgtttcggtg ctgatcgggg agctggcaga cgcctttggc atcttcattg ctgtctgcac agtcaacacg gtgggcttca ttcaggagta ccggtcagag aagagcgtgg aagcgctgca gaacttggtg gcccatcgca cacacgtcct gcgggacggc cagcccaggg aagtgctggc ggagagcctt gtgcctgggg atttggtggt gctgaacgtg ggcgaccgcg tgccggcaga ccttcgtctc attgagacca taaacctgga gctggacgag tccattttga cgggagagac agaaccttct gtcaagtgca cagaacaact ttcttcttcc tcttcttcct cttcttcctc ttcttctgtg cctgagggtg gcattttgga aggacggccg aggcgagaca cggaagagtt ccaactggca gaccgctcca acatggcgta catgggcaca atcaccgtca atggcaaggg caagggcctc gtggtggcca ctagtaccgc cactgaactg ggcaggatct gggaggccat caccagcatg ggcgagaagc gcacgcccct ccagctgaaa atggatcaac tgggcaagca gctctccctt gcggccttcg tcatcgtggg agtcattttt attctgggag ccatacaggg caagcctttg ttgcaaatgt tcacgatcgg agtgagcttg gctgtggccg ccattcctga gggactgccc atcgtggtga cagtcacgtt ggccttggga gtgacccgca tggctaatcg tcacgctatc gtgcggaagc tccctgccgt ggaggcattg ggagccacca ccgccatttg cagcgacaag acgggcacgc tgaccaagaa cgagatgacg gtgcggcgcc tgttcactac ggagttcatt tcagtcagcg gggagggcta tcatcaccag ttctggcagc agccccaagg agagttcttc agggaggggt gtgaccagcc gtttgaccct gctcaggatc ctcatctcat gcaactgatt caggttggcg caatctgtaa caacgcgcac cttcgcgagg atggtgtgct gggtcaaccc actgaagtgg ctctgttggt attagctcgc aaagctggca tcgaaatcga tcttcgcacg ttatttcctc gtcagtcaga agaccccttc acccacgcca gcaaacgcat gtccgtgctt tgcgactttc ctcctgacgc tcgcaagatg accacttttc acgtttcttc ttctttctct tcgtcttcct cttcacaacc catttacttc atgaaaggag ctcttcccgt tgtgttggac cactgtacca ccattagcaa cgctcaaggc aacattattg atctcactcc acagaagcaa gagatgctcc tcgctgctga gaaggagatg ggagaaaacg cccttcgggt agtggccgtt gcctacggaa ctagctcgcg gtccatgaac ttggtaggtt tgtgtggcat catggatccg cctagaaacg gcgttttaga agccattagg gaagtacaac aaacgggcgt gcacgtagca atggtaactg gcgactcccg cgaaaccgcc atcgccatcg cccgcgagct ctccttcttc catcgcgaca gcctggccct ctcctccgcc gatttacaac gcctgagcga ggaggagctg gccagccaga tcgagcgggt ggccgtcttt tatcgcatgt cccccgagga caagatgaag atcgtcaagg cctacaagaa caggggccac atcgtggcgg tcactggaga tggcgtcaac gacgctcccg ccctgaagat cgccgacatt ggtatcgcca tgggcaaagg aacagacgtc tgccgagagg cggcggagat gatcctggtg gacgataact ttgccactat agtggccgcc atagaggagg gcaagtccat ctacaacaac atcaagaact ttttgcggtt ccagctaacc accagcattg ccactctgtc cattatcgcc tcctccaccc tgtttggtct gcctttgccc atgaacccca ttcaaatctt gtggatcaat atcattatgg atggcccacc cgctcaaagc ttgggtgtgg aacctttgga taaggatgcc atgaagcaac ctccgcgaga caccaagcaa cctgttctct ccaagccgct cctcttctct accctagcct gtgcactgct catgctcttc ggtacccttg gcatctacca atcttacctc gcggatcctt catatgacat tggagaggga gacgaggaag cagcagctca acgcactctc aaagcctcca cgatggcctt taacaccttt gtcttcttcc agatgttcaa cgcggtcaac tgtcgctccg accacaagag cgccttccaa atcaagttca tgaacaacaa gttcttcctt ctggccatcg gtggcagttt gttgctgcaa ctggcggcca tctacgtgcc catcctccag atgctcttcg agactaccag tctctccatg catgagttac tgcagtgcct tatggtctcc tcctctgtct ttgggttgga cgaggcccgc aagtacttcc tcaaagccca gcaagaaggc gctgttcgat ttaatgtctg a
Details for BamaA.18982.a.B2.GE43479
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 43kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA NRHAIVRKLP AVEALGATTA ICSDKTGTLT KNEMTVRRLF TTEFISVSGE GYHHQFWQQP QGEFFREGCD QPFDPAQDPH LMQLIQVGAI CNNAHLREDG VLGQPTEVAL LVLARKAGIE IDLRTLFPRQ SEDPFTHASK RMSVLCDFPP DARKMTTFHV SSSFSSSSSS QPIYFMKGAL PVVLDHCTTI SNAQGNIIDL TPQKQEMLLA AEKEMGENAL RVVAVAYGTS SRSMNLVGLC GIMDPPRNGV LEAIREVQQT GVHVAMVTGD SRETAIAIAR ELSFFHRDSL ALSSADLQRL SEEELASQIE RVAVFYRMSP EDKMKIVKAY KNRGHIVAVT GDGVNDAPAL KIADIGIAMG KGTDVCREAA EMILVDDN
Validated NT Sequence
ccgccgcctc tcggcagacg tctgttcctt tgcccatggc gataccaatg tcggcgatct tcagggcggg agcgtcgttg acgccatctc cagtgaccgc cacgatgtgg cccctgttct tgtaggcctt gacgatcttc atcttgtcct cgggggacat gcgataaaag acggccaccc gctcgatctg gctggccagc tcctcctcgc tcaggcgttg taaatcggcg gaggagaggg ccaggctgtc gcgatggaag aaggagagct cgcgggcgat ggcgatggcg gtttcgcggg agtcgccagt taccattgct acgtgcacgc ccgtttgttg tacttcccta atggcttcta aaacgccgtt tctaggcgga tccatgatgc cacacaaacc taccaagttc atggaccgcg agctagttcc gtaggcaacg gccactaccc gaagggcgtt ttctcccatc tccttctcag cagcgaggag catctcttgc ttctgtggag tgagatcaat aatgttgcct tgagcgttgc taatggtggt acagtggtcc aacacaacgg gaagagctcc tttcatgaag taaatgggtt gtgaagagga agacgaagag aaagaagaag aaacgtgaaa agtggtcatc ttgcgagcgt caggaggaaa gtcgcaaagc acggacatgc gtttgctggc gtgggtgaag gggtcttctg actgacgagg aaataacgtg cgaagatcga tttcgatgcc agctttgcga gctaatacca acagagccac ttcagtgggt tgacccagca caccatcctc gcgaaggtgc gcgttgttac agattgcgcc aacctgaatc agttgcatga gatgaggatc ctgagcaggg tcaaacggct ggtcacaccc ctccctgaag aactctcctt ggggctgctg ccagaactgg tgatgatagc cctccccgct gactgaaatg aactccgtag tgaacaggcg ccgcaccgtc atctcgttct tggtcagcgt gcccgtcttg tcgctgcaaa tggcggtggt ggctcccaat gcctccacgg cagggagctt ccgcacgata gcgtgacgat tagccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMA NRHAIVRKLP AVEALGATTA ICSDKTGTLT KNEMTVRRLF TTEFISVSGE GYHHQFWQQP QGEFFREGCD QPFDPAQDPH LMQLIQVGAI CNNAHLREDG VLGQPTEVAL LVLARKAGIE IDLRTLFPRQ SEDPFTHASK RMSVLCDFPP DARKMTTFHV SSSFSSSSSS QPIYFMKGAL PVVLDHCTTI SNAQGNIIDL TPQKQEMLLA AEKEMGENAL RVVAVAYGTS SRSMNLVGLC GIMDPPRNGV LEAIREVQQT GVHVAMVTGD SRETAIAIAR ELSFFHRDSL ALSSADLQRL SEEELASQIE RVAVFYRMSP EDKMKIVKAY KNRGHIVAVT GDGVNDAPAL KIADIGIAMG KGTDVCREAA EMILVDDN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta atcgtcacgc tatcgtgcgg aagctccctg ccgtggaggc attgggagcc accaccgcca tttgcagcga caagacgggc acgctgacca agaacgagat gacggtgcgg cgcctgttca ctacggagtt catttcagtc agcggggagg gctatcatca ccagttctgg cagcagcccc aaggagagtt cttcagggag gggtgtgacc agccgtttga ccctgctcag gatcctcatc tcatgcaact gattcaggtt ggcgcaatct gtaacaacgc gcaccttcgc gaggatggtg tgctgggtca acccactgaa gtggctctgt tggtattagc tcgcaaagct ggcatcgaaa tcgatcttcg cacgttattt cctcgtcagt cagaagaccc cttcacccac gccagcaaac gcatgtccgt gctttgcgac tttcctcctg acgctcgcaa gatgaccact tttcacgttt cttcttcttt ctcttcgtct tcctcttcac aacccattta cttcatgaaa ggagctcttc ccgttgtgtt ggaccactgt accaccatta gcaacgctca aggcaacatt attgatctca ctccacagaa gcaagagatg ctcctcgctg ctgagaagga gatgggagaa aacgcccttc gggtagtggc cgttgcctac ggaactagct cgcggtccat gaacttggta ggtttgtgtg gcatcatgga tccgcctaga aacggcgttt tagaagccat tagggaagta caacaaacgg gcgtgcacgt agcaatggta actggcgact cccgcgaaac cgccatcgcc atcgcccgcg agctctcctt cttccatcgc gacagcctgg ccctctcctc cgccgattta caacgcctga gcgaggagga gctggccagc cagatcgagc gggtggccgt cttttatcgc atgtcccccg aggacaagat gaagatcgtc aaggcctaca agaacagggg ccacatcgtg gcggtcactg gagatggcgt caacgacgct cccgccctga agatcgccga cattggtatc gccatgggca aaggaacaga cgtctgccga gaggcggcgg agatgatcct ggtggacgat aactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat