BamaA.17129.a

Heat shock protein 90 (HSP90)

CENTER ID: BamaA.17129.a
ORGANISM: Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamaA.17129.a.B1.GE43478 full length 1 709
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEEVETFAF QAEINQLLSL IINTFYSNKE IFLRELISNS SDALDKIRYQ SLTDKAQLES NPEFYIKIVP DKANNALNII DSGIGMTKAD LVNNLGTIAK SGTKAFMEAL QAGADVSMIG QFGVGFYSSY LVAEKVVVTT KHNDDEQLVW ESAAGGSFTI RKDTSGEELK RGTKVTLYLK DDQQEYLEER RLKDLVKKHS QFIQYPIQLY VEKEVEKEVS DDEAEEKKEE EEKKEGEEPK IEEVTEEEDK KEGEKKKKKV KETTHEWELL NKTKPIWTRN PKDVTPEEYK TFYKSLTNDW EEHLAVKHFS VEGQLEFKSI IYVPKRAPFD LFETRKKLNN IKLYVRRVFI MDNCEELIPE WLNFIKGIVD SEDLPLNISR EMLQQNKILR VIRKNIVKKC IELFNEIAEN KEDFDKFYES FGKNIKYGIH EDSQNRTKLA ELLRFHSTTS GDNTTSFKDY ITNMKEGQKD IYFISGESRK AVENSPFLEG LKKRGYEVLF FTDPIDEYMV QQLKEIDGHK LVNVTKEGLK LDETEDEKKK AEEEKKANEN LLKVIKEILG DKVEKVVLSS RLVNSPCVLV TGEFGWSANM ERIMKAQALR DNSMSTYMVS KKTLEINPSH SIVSSLRQKA DADKSDKTVK DLVWLLFETA LLSSGFSLDD PSSFASRIHR MIKLGLSIDE DEEPAMEADL PPLEEEEGGD EGSKMEEVD
NT Sequence
atgtccgaag aagtagagac ctttgctttc caggccgaga tcaaccagct gttgagcttg atcatcaaca ccttctacag caacaaggaa attttcttgc gtgagctcat ctccaactcc tctgacgcct tggacaagat ccgctaccag agcctcactg acaaggccca actcgagtcc aaccccgagt tctacatcaa gatcgtcccc gacaaggcca acaatgccct caacatcatc gactctggta ttggcatgac caaggccgac ctcgtgaaca acctcggtac catcgccaag tccggcacca aggccttcat ggaggctctc caggctggtg ctgacgtcag catgattggt cagttcggtg tgggcttcta ctcttcctac ctcgtggctg agaaagttgt ggttaccacc aagcacaacg atgacgagca actcgtttgg gagtctgccg ctggcggttc tttcaccatc cgcaaggata cctctggtga ggaattgaag agaggcacca aggtcaccct ttacctcaag gacgaccaac aggagtattt ggaggagcgc cgcctgaagg acctcgtcaa gaagcactct cagttcatcc agtaccccat ccaactctat gttgagaagg aggtcgaaaa ggaggtctct gacgacgaag cggaagagaa gaaggaggag gaagagaaga aggaaggcga ggagccgaag atcgaggaag tgaccgagga ggaggataag aaggaaggcg agaagaagaa aaagaaggtc aaggagacca cccacgaatg ggagctgctc aacaagacca agcctatctg gacccgtaat cccaaggacg ttacccccga ggagtacaag actttctaca agagcttgac caacgactgg gaggagcact tggctgtcaa gcatttctcc gtggagggtc agctggagtt caagtccatc atctacgtgc ccaagcgcgc tcccttcgac ctcttcgaga ctcgcaagaa gctcaacaac atcaagctct acgtccgccg tgtgttcatc atggacaact gcgaggagct catccccgag tggctcaact tcatcaaggg tatcgtcgac tccgaggact tgcccctcaa catctctcgt gagatgcttc aacagaacaa gatcctccgc gtgatccgca agaacatcgt caagaagtgc attgagttgt tcaacgagat tgctgagaac aaggaagact ttgacaagtt ctacgagtcc tttggcaaga acatcaagta cggcatccac gaggacagcc agaacaggac caagttggcc gagttgctcc gcttccactc caccacctct ggcgacaaca ccacttcctt caaagactac atcaccaaca tgaaggaggg ccagaaggat atctacttca tcagcggtga gagcaggaag gccgttgaga actctccctt cttggagggc ctcaagaagc gcggctacga ggtgctcttc ttcactgacc ccatcgacga gtacatggtg cagcaactca aggagattga cggccacaag cttgtcaacg tcaccaagga gggcctcaag ctcgacgaaa ccgaagacga gaagaagaag gccgaagagg agaagaaggc taacgagaac ctgctcaagg tcatcaagga gatcctcggc gacaaggtcg agaaggttgt gctctcttcc cgcctcgtca actccccttg tgtcctcgtc accggtgagt ttggctggtc cgccaacatg gagaggatca tgaaggccca ggccctccgc gacaacagca tgtccaccta catggtttcc aagaagaccc tcgagatcaa cccctctcac tcgatcgttt cttccctcag gcagaaggcc gacgctgaca agagcgataa gaccgtcaag gaccttgtct ggttgctctt cgagactgcc ctcttgtcct ctggcttctc cctggacgac ccatccagct ttgcttcccg catccaccgc atgatcaagc ttggtctcag catcgatgag gacgaggaac ccgccatgga ggctgacctg ccccccttgg aggaggaaga aggtggcgat gagggctcca agatggagga ggttgactaa
Details for BamaA.17129.a.B1.GE43478
HARVESTED ON: 11/19/2019
SEQUENCED ON: 2/5/2020
EXPECTED MW: 83kDa
OBSERVED MW: 83kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 100
Validated AA Sequence
XXHHHHXHMS XXVETXAFXA EINQXLSLII XTFYXXXEIF LRELISXSSD XLDXIXXQXL XDKXXXXSXP EFYIXIXPXK AXXALXXIXS GIXXTXADXX NXLXTIXXSG TXAFMXALQA GADVSMIGQF GVGFYSSYLV AEKVVVTTKH NDDEQLVWES AAGGSFTIRK DTSGEELKRG TKVTLYLKDD QQEYLEERRL KDLVKKHSQF IQYPIQLYVE KEVEKEVSDD EAEEKKEEEE KKEGEEPKIE EVTEEEDKKE GEKKKKKVKE TTHEWELLNK TKPIWTRNPK DVTPEEYKTF YKSLTNDWEE HLAVKHFSVE GQLEFKSIIY VPKRAPFDLF ETRKKLNNIK LYVRRVFIMD NCEELIPEWL NFIKGIVDSE DLPLNISREM LQQNKILRVI RKNIVKKCIE LFNEIAENKE DXDKFYESFG KNIKYGIHED SQNRTKLAEL LRFHSTTSGD NTTSFKDYIT NMKEGQKDIY FISGESRKAV ENSPFLEGLK KRGYEVLFFT DPIDEYMVQQ LKEIDGHKLV NVTKEGLKLD ETEDEKKKAE EEKKANENLL KVIKEILGDK VEKVVLSSRL VNSPCVLVTG EFGWSANMER IMKAQALRDN SMSTYMVSKK TLEINPSHSI VSSLRQKADA DKSDKTVKDL VWLLFETALL SSGFSLDDPS SFASRIHRMI KLGLSIDEDE EPAMEADLPP LEEEEGGDEG SKMEEVD
Validated NT Sequence
rtggstcacc accaccacma ccatatgtcc kaasaagtag agaccttkgc tttccakgcc gagatcaacc agstgttgag cttgatcatc wwcacsttct acrgcracrr ggaaattttc ttgcgtgagc tcatctcsmw ctcctckgac sccttggacw rratcmgcta scaragmctc wctgacaagg yccwrstsra gtccwacccc gagttctaca tcawgatckt ccccrasaag gccaamaakg ccctcwrcwt catckrctct ggtattgscw tgacsawkgc cgacckcgkg aacmamctcr gwacsatcgy smwgtcyggc acyraggcct tcatggakgc tctccaggct ggtgctgacg tcagcatgat tggtcagttc ggtgtgggct tctactcttc ctacctcgtg gctgagaaag ttgtggttac caccaagcac aacgatgacg agcaactcgt ttgggagtct gccgctggcg gttctttcac catccgcaag gatacctctg gtgaggaatt gaagagaggc accaaggtca ccctttacct taaggacgac caacaggagt atttggagga gcgccgcctg aaggacctcg tcaagaagca ctctcagttc atccagtacc ccatccaact ctatgttgag aaggaggtcg aaaaggaggt ctctgacgac gaagcggaag agaagaagga ggaggaagag aagaaggaag gcgaggagcc gaagatcgag gaagtgaccg aggaggagga caagaaggaa ggcgagaaga agaagaagaa ggtcaaggag accacccacg aatgggagct gctcaacaag accaagccta tctggacccg taatcccaag gacgttaccc ccgaggagta caagactttc tacaagagct tgaccaacga ctgggaggag cacttggctg tcaagcattt ctccgtggag ggtcagctgg agttcaagtc catcatctac gtgcccaagc gcgctccctt cgacctcttc gagactcgca agaagctcaa caacatcaag ctctacgtcc gccgtgtgtt catcatggac aactgcgagg agctcatccc cgagtggctc aacttcatca agggtatcgt cgactccgag gacttgcccc tcaacatctc tcgtgagatg cttcaacaga acaagatcct ccgcgtgatc cgcaagaaca tcgtcaagaa gtgcattgag ttgttcaacg agattgctga gaacaagg-a agacttngac aagttctacg agtcctttgg caagaacatc aagtacggca tccacgagga cagccagaac aggaccaagt tggccgagtt gctccgcttc cactccacca cctctggcga caacaccact tccttcaaag actacatcac caacatgaag gagggccaga aggatatcta cttcatcagc ggtgagagca ggaaggccgt tgagaactct cccttcttgg agggcctcaa gaagcgcggc tacgaggtgc tcttcttcac tgaccccatc gacgagtaca tggtgcagca actcaaggag attgacggcc acaagcttgt caacgtcacc aaggagggcc tcaagctcga cgaaaccgaa gacgagaaga agaaggccga agaggagaag aaggctaacg agaacctgct caaggtcatc aaggagatcc tcggcgacaa ggtcgagaag gttgtgctct cttcccgcct cgtcaactcc ccttgtgtcc tcgtcaccgg tgagtttggc tggtccgcca acatggagag gatcatgaag gcccaggccc tccgcgacaa cagcatgtcc acctacatgg tttccaagaa gaccctcgag atcaacccct ctcactcgat cgtttcttcc ctcaggcaga aggccgacgc tgacaagagc gataagaccg tcaaggacct tgtctggttg ctcttcgaga ctgccctctt gtcctctggc ttctccctgg acgacccatc cagctttgct tcccgcatcc accgcatgat caagcttggt ctcagcatcg atgaggacga ggaacccgcc atggaggctg acctgccccc cttggaggag gaagaaggtg gcgatgaggg ctccaagatg gaggaggttg actaa
Expected Protein Sequence
MAHHHHHHMS EEVETFAFQA EINQLLSLII NTFYSNKEIF LRELISNSSD ALDKIRYQSL TDKAQLESNP EFYIKIVPDK ANNALNIIDS GIGMTKADLV NNLGTIAKSG TKAFMEALQA GADVSMIGQF GVGFYSSYLV AEKVVVTTKH NDDEQLVWES AAGGSFTIRK DTSGEELKRG TKVTLYLKDD QQEYLEERRL KDLVKKHSQF IQYPIQLYVE KEVEKEVSDD EAEEKKEEEE KKEGEEPKIE EVTEEEDKKE GEKKKKKVKE TTHEWELLNK TKPIWTRNPK DVTPEEYKTF YKSLTNDWEE HLAVKHFSVE GQLEFKSIIY VPKRAPFDLF ETRKKLNNIK LYVRRVFIMD NCEELIPEWL NFIKGIVDSE DLPLNISREM LQQNKILRVI RKNIVKKCIE LFNEIAENKE DFDKFYESFG KNIKYGIHED SQNRTKLAEL LRFHSTTSGD NTTSFKDYIT NMKEGQKDIY FISGESRKAV ENSPFLEGLK KRGYEVLFFT DPIDEYMVQQ LKEIDGHKLV NVTKEGLKLD ETEDEKKKAE EEKKANENLL KVIKEILGDK VEKVVLSSRL VNSPCVLVTG EFGWSANMER IMKAQALRDN SMSTYMVSKK TLEINPSHSI VSSLRQKADA DKSDKTVKDL VWLLFETALL SSGFSLDDPS SFASRIHRMI KLGLSIDEDE EPAMEADLPP LEEEEGGDEG SKMEEVD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg aagaagtaga gacctttgct ttccaggccg agatcaacca gctgttgagc ttgatcatca acaccttcta cagcaacaag gaaattttct tgcgtgagct catctccaac tcctctgacg ccttggacaa gatccgctac cagagcctca ctgacaaggc ccaactcgag tccaaccccg agttctacat caagatcgtc cccgacaagg ccaacaatgc cctcaacatc atcgactctg gtattggcat gaccaaggcc gacctcgtga acaacctcgg taccatcgcc aagtccggca ccaaggcctt catggaggct ctccaggctg gtgctgacgt cagcatgatt ggtcagttcg gtgtgggctt ctactcttcc tacctcgtgg ctgagaaagt tgtggttacc accaagcaca acgatgacga gcaactcgtt tgggagtctg ccgctggcgg ttctttcacc atccgcaagg atacctctgg tgaggaattg aagagaggca ccaaggtcac cctttacctc aaggacgacc aacaggagta tttggaggag cgccgcctga aggacctcgt caagaagcac tctcagttca tccagtaccc catccaactc tatgttgaga aggaggtcga aaaggaggtc tctgacgacg aagcggaaga gaagaaggag gaggaagaga agaaggaagg cgaggagccg aagatcgagg aagtgaccga ggaggaggat aagaaggaag gcgagaagaa gaaaaagaag gtcaaggaga ccacccacga atgggagctg ctcaacaaga ccaagcctat ctggacccgt aatcccaagg acgttacccc cgaggagtac aagactttct acaagagctt gaccaacgac tgggaggagc acttggctgt caagcatttc tccgtggagg gtcagctgga gttcaagtcc atcatctacg tgcccaagcg cgctcccttc gacctcttcg agactcgcaa gaagctcaac aacatcaagc tctacgtccg ccgtgtgttc atcatggaca actgcgagga gctcatcccc gagtggctca acttcatcaa gggtatcgtc gactccgagg acttgcccct caacatctct cgtgagatgc ttcaacagaa caagatcctc cgcgtgatcc gcaagaacat cgtcaagaag tgcattgagt tgttcaacga gattgctgag aacaaggaag actttgacaa gttctacgag tcctttggca agaacatcaa gtacggcatc cacgaggaca gccagaacag gaccaagttg gccgagttgc tccgcttcca ctccaccacc tctggcgaca acaccacttc cttcaaagac tacatcacca acatgaagga gggccagaag gatatctact tcatcagcgg tgagagcagg aaggccgttg agaactctcc cttcttggag ggcctcaaga agcgcggcta cgaggtgctc ttcttcactg accccatcga cgagtacatg gtgcagcaac tcaaggagat tgacggccac aagcttgtca acgtcaccaa ggagggcctc aagctcgacg aaaccgaaga cgagaagaag aaggccgaag aggagaagaa ggctaacgag aacctgctca aggtcatcaa ggagatcctc ggcgacaagg tcgagaaggt tgtgctctct tcccgcctcg tcaactcccc ttgtgtcctc gtcaccggtg agtttggctg gtccgccaac atggagagga tcatgaaggc ccaggccctc cgcgacaaca gcatgtccac ctacatggtt tccaagaaga ccctcgagat caacccctct cactcgatcg tttcttccct caggcagaag gccgacgctg acaagagcga taagaccgtc aaggaccttg tctggttgct cttcgagact gccctcttgt cctctggctt ctccctggac gacccatcca gctttgcttc ccgcatccac cgcatgatca agcttggtct cagcatcgat gaggacgagg aacccgccat ggaggctgac ctgcccccct tggaggagga agaaggtggc gatgagggct ccaagatgga ggaggttgac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat