BamaA.10201.a

Methionyl-tRNA synthetase (methionine tRNA ligase) (MetRS)

CENTER ID: BamaA.10201.a
ORGANISM: Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamaA.10201.a.B1.GE43477 full length 1 723
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEDKPSREAL EAEIAALKEA QKEVQDKMLA LKKAKVTGSE LDDVLAQLKD GKAKIAEQEK VLKALYPEAV ESKKGKSKKE KTGNAPAASA KNKKKEVPKK KVELYQMPEG GLPDVGDQTG KEPVLPQKGK RNVLITSALP YVNNVPHLGN IIGCVLSADV FARYCRLRGY NTIYICGTDE YGTATEIKAM QEGLSEMEIC NKYHAMHKQA YEWFGCQFDK FGRTTTESQT RIAQDIFTKI HARNYLVEKT LDQLWCAHCQ RYLSDRYVEG TCPRPDCRYE DARGDQCDKC GRCYDAMELV NPRCKFCSNT PEIKREEHLF LDLPKLEDPL SKWVDKSSKE GIWSPNAINI TTKTWLAGGL QPRCITRDLK WGTPVPLEKY KNKVFYVWFD APIGYLSITA EYTEQWELWW KNPEEYDVKL YQFMGKDNVP FHTVIFPASL MATGDNWTLL DSVCTTEYLN YEGEQFSKSR GVGIFGDHVQ KTGIPPSVWR YYLLSNRPES SDSSFSWSDL VAKNNNELLA NLGNFVNRCL KFTADRLDKT VSASFDPERD LLPTEDKEQF LDKVNEHLRQ YVEALEATKL KDGLFQAMQI SKLGNQYLQD QKPWVLLSEN RKRCEVVCAV AVNVVYLLAR LLEPYIPHFS EMVLHQLALP HGLIPDRFAF EVPAGHVIRT PLPIFKELTE GEVAEIRSKI DVNPVFLERL KQKQAQGSSS SSAATAGSSK TAE
NT Sequence
atggaagaca agccaagcag agaagcgctg gaagcggaga ttgctgcctt gaaggaggcg cagaaggagg tgcaggacaa gatgttggcc ctcaagaaag ccaaggtcac tggctcggaa ctggatgatg ttttggctca acttaaggac ggcaaagcta agattgctga gcaggaaaaa gtgctcaagg cactctatcc ggaagctgtt gagtcaaaga agggcaaatc aaagaaagaa aagaccggca atgcccctgc cgctagtgcc aaaaacaaga aaaaagaagt acccaagaag aaggtggagc tttatcaaat gcctgaagga ggccttcccg atgttggaga ccaaactggc aaagaaccag tgctccctca aaagggtaaa cgcaacgttc tcatcacaag cgctcttccc tacgtcaata acgtgcctca tttgggcaac atcatcggct gcgttttgag tgctgatgtc tttgctcggt actgccgttt gaggggctat aacaccatct acatttgtgg cacggatgag tacggcactg ccactgaaat caaggccatg caagagggat taagcgagat ggagatctgt aacaagtatc acgccatgca caaacaagcg tacgagtggt ttggctgcca attcgataag tttggacgta ctaccaccga aagtcaaact cggatcgcgc aagatatctt caccaagatt catgcaagga actaccttgt ggagaaaacg ctagatcaac tgtggtgcgc ccattgccaa cgttatctat cggatcgtta cgtggaaggc acatgtccaa gacctgattg tcgttatgag gacgcaagag gagatcaatg tgacaaatgt ggccgttgct atgatgccat ggagttggtt aacccgcgct gcaagttttg ctcgaatacg cctgaaataa agcgagaaga gcacctgttc ctggacttgc ccaaactcga agatcctctt tccaagtggg tcgacaaatc aagtaaggaa ggtatctggt ccccgaatgc catcaacatc acgaccaaga cctggttggc tggtggcttg caacctcgtt gtatcacaag agatcttaag tggggtactc ctgtaccatt ggaaaagtac aagaacaaag tcttttacgt atggttcgac gcgcccattg gatacctttc catcactgct gaatacaccg agcaatggga gttgtggtgg aagaatccgg aagagtatga cgtcaagctc taccaattca tgggcaagga caacgtgccc ttccacacgg taatcttccc ggccagtctc atggccaccg gcgataactg gaccctcctc gatagcgttt gcaccaccga gtacctcaac tacgagggag agcaattctc caagagcaga ggagtgggca tctttggaga ccacgtccaa aagactggca tccctccctc tgtctggcga tactacctgc tctccaacag acccgagtcc tccgacagct ccttctcctg gtcggatctg gtggccaaga acaacaacga gctgctggcc aacctgggca actttgtgaa ccgctgcctc aagttcaccg ccgaccgctt ggacaagacc gtctctgcct ccttcgatcc agagagggac ctcctcccta ctgaggacaa ggagcaattc ctggacaagg tgaacgagca tttgagacag tacgtggagg ccctggaagc caccaagctc aaggatggcc tctttcaggc catgcaaatc tccaagctgg gcaaccaata cctccaggac caaaagccat gggtccttct ctccgaaaac cgaaagcgtt gcgaggtggt ctgcgccgtc gctgtgaacg tggtgtacct cctggccagg ttgttggagc cctacatacc gcatttctcc gagatggtgc tgcaccagct ggccctgccg cacggcctga tccccgaccg tttcgcgttc gaagtgcccg ccggccatgt catcaggacg cccttgccca ttttcaagga gctgacggag ggggaggtgg ccgagatccg cagcaagatt gacgttaacc cggtcttcct ggagaggctg aagcagaagc aagcgcaagg ctcctcctcc tcctctgctg ctaccgctgg ttcctcgaag acggccgaat ga
Details for BamaA.10201.a.B1.GE43477
HARVESTED ON: 11/19/2019
SEQUENCED ON: 2/5/2020
EXPECTED MW: 83kDa
OBSERVED MW: 83kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHXHXHMX XXXXRXXLEA EIAALKEAQK XVXDXXXXLX KXXVXXSXLD DVLXQXXXGK XXIXEXEXXL XXLYPEXXEX XKGKSKKEXT GNAPAXXXXX XXXXVXXXXV XLYXXPXGXL XGXXXXTDXE PXXPQKGXXX XLITXXLXYV XNVPHLGNII GCVLSADVFA RYCRXRGYNT IYICGTDEYG TATEIKAMQX GXXEMEICNX YHAMHKQAYE WFGCQFDKFG RTTTESQTRI AQDIFTXIHA RNYLVEKTLD QLWCAHCQRY LSDRYVEGTC PRPDCRYEDA RGDQCDKCGR CYDAMELVNP RCKFCSNTPE IKREEHLFLD LPKLEDPLSK WVDKSSKEGI WSPNAINITT KTWLAGGLQP RCITRDLKWG TPVPLEKYKN KVFYVWFDAP IGYLSITAEY TEQWELWWKN PEEYDVKLYQ FMGKDNVPFH TVIFPASLMA TGDNWTLLDS VCTTEYLNYE GEQFSKSRGV GIFGDHVQKT GIPPSVWRYY LLSNRPESSD SXFSWSDLVA KNNNELLANL GNFVNRCLKF TADRLDKTVS ASFDPERDLL PTEDKEQFLD KVNEHLRQYV EALEATKLKD GLFQAMQISK LGNQYLQDQK PWVLLSENRK RCEVVCAVAV NVVYLLARLL EPYIPHFSEM VLHQLALPHG LIPDRFAFEV PAGHVIRTPL PIFKELTEGE VAEIRSKIDV NPVFLERLKQ KQAQGSSSSS AATAGSSKTA E
Validated NT Sequence
mtggctcacc accascacma ycatatgkaa kacamgycaa rcagasaanc gctggaagcg gagattgctg ccttgaagga ggcgcagaag gasgtgmagg acragawgkt grccctcwag aaakscwwsg tcastgkstc sgamytggat gatgttttgg stcaamttaw ggamggmaaa kctamgattg ytgagswgga aawrktgctc awrgswctct atccggaakc tgktgagwca waraarggsa aatcaaagaa agaaawgacc ggcaatgccc ckgccgmtas tgmcmwrmas mmsamaamak aagtacscaa kaasamkgtg sagctttayy aaakgccyga wggargcctt cysggkgktg rasaccwrac tgayamagaa ccmsygckcc ctcaaaaggg tawwykcawc wkcctsatca cmarckstct tmcctacgty nataacgtgc ctcatttggg caacatcatc ggctgcgttt tgagtgctga tgtctttgct cggtactgcc gttngagggg ctataacacc atctacattt gtggcacgga tgagtacggc actgccactg aaatcaaggc catgcaagan ggatwaakcg agatggagat ctgtaacmag tatcacgcca tgcacaaaca agcgtacgag tggtttggct gccaattcga taagtttgga cgtactacca ccgaaagcca aactcggatt gcgcaagata tcttcaccra gattcatgca aggaactacc ttgtggagaa aacgctagat caactgtggt gcgcccattg ccaacgttat ctgtcagatc gttacgtgga aggtacatgc ccaagacctg attgtcgtta tgaggacgca agaggagatc aatgtgacaa atgtggccgt tgctatgatg ccatggagtt ggttaacccg cgctgcaagt tttgctcgaa tacgcctgaa ataaagcgag aagagcatct gttcctggat ttgcccaaac tcgaagatcc tctttccaag tgggtcgaca aatcaagtaa ggaaggtatc tggtccccga atgccatcaa catcacgacc aagacctggt tggctggtgg cttgcaacct cgttgtatca caagagatct taagtggggt actcctgtac cattggaaaa gtacaagaac aaagtctttt acgtatggtt cgacgcgccc attggatacc tttccatcac tgctgaatac accgagcaat gggagttgtg gtggaagaat ccggaagagt atgacgtcaa gctctaccaa ttcatgggca aggacaacgt gcccttccac acggtaatct tcccggccag tctcatggcc accggtgaca actggaccct cctcgatagc gtttgcacca ccgagtacct caactacgag ggagagcaat tctccaagag cagaggagtg ggcatctttg gagaccacgt ccaaaagact ggcatccctc cctctgtctg gcgatactac ctgctctcca acagacccga gtcntccgac agctnnttct cctggtcgga tctggtggcc aagaacaaca acgagctgct ggccaacctg ggcaactttg tgaaccgctg cctcaagttc accgccgacc gcttggacaa gaccgtctct gcctccttcg atccagagag ggacctcctc cctactgagg acaaggagca attcctggac aaggtgaacg agcatttgag acagtacgtg gaggccctgg aagccaccaa gctcaaggat ggcctctttc aggccatgca aatctccaag ctgggcaacc aatacctcca ggaccaaaag ccatgggtcc ttctctccga aaaccgaaag cgttgcgagg tggtctgcgc cgtcgctgtg aacgtggtgt acctcctggc caggttgttg gagccctaca taccgcattt ctccgagatg gtgctgcacc agctggccct gccgcacggc ctgatccccg accgtttcgc gttcgaagtg cccgccggcc atgtcatcag gacgcccttg cccattttca aggagctgac ggagggggag gtggccgaga tccgcagcaa gattgacgtt aacccggtct tcctggagag gctgaagcag aagcaagcgc aaggctcctc ctcctcctct gctgctaccg ctggttcctc gaagacggcc gaataw
Expected Protein Sequence
MAHHHHHHME DKPSREALEA EIAALKEAQK EVQDKMLALK KAKVTGSELD DVLAQLKDGK AKIAEQEKVL KALYPEAVES KKGKSKKEKT GNAPAASAKN KKKEVPKKKV ELYQMPEGGL PDVGDQTGKE PVLPQKGKRN VLITSALPYV NNVPHLGNII GCVLSADVFA RYCRLRGYNT IYICGTDEYG TATEIKAMQE GLSEMEICNK YHAMHKQAYE WFGCQFDKFG RTTTESQTRI AQDIFTKIHA RNYLVEKTLD QLWCAHCQRY LSDRYVEGTC PRPDCRYEDA RGDQCDKCGR CYDAMELVNP RCKFCSNTPE IKREEHLFLD LPKLEDPLSK WVDKSSKEGI WSPNAINITT KTWLAGGLQP RCITRDLKWG TPVPLEKYKN KVFYVWFDAP IGYLSITAEY TEQWELWWKN PEEYDVKLYQ FMGKDNVPFH TVIFPASLMA TGDNWTLLDS VCTTEYLNYE GEQFSKSRGV GIFGDHVQKT GIPPSVWRYY LLSNRPESSD SSFSWSDLVA KNNNELLANL GNFVNRCLKF TADRLDKTVS ASFDPERDLL PTEDKEQFLD KVNEHLRQYV EALEATKLKD GLFQAMQISK LGNQYLQDQK PWVLLSENRK RCEVVCAVAV NVVYLLARLL EPYIPHFSEM VLHQLALPHG LIPDRFAFEV PAGHVIRTPL PIFKELTEGE VAEIRSKIDV NPVFLERLKQ KQAQGSSSSS AATAGSSKTA E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag acaagccaag cagagaagcg ctggaagcgg agattgctgc cttgaaggag gcgcagaagg aggtgcagga caagatgttg gccctcaaga aagccaaggt cactggctcg gaactggatg atgttttggc tcaacttaag gacggcaaag ctaagattgc tgagcaggaa aaagtgctca aggcactcta tccggaagct gttgagtcaa agaagggcaa atcaaagaaa gaaaagaccg gcaatgcccc tgccgctagt gccaaaaaca agaaaaaaga agtacccaag aagaaggtgg agctttatca aatgcctgaa ggaggccttc ccgatgttgg agaccaaact ggcaaagaac cagtgctccc tcaaaagggt aaacgcaacg ttctcatcac aagcgctctt ccctacgtca ataacgtgcc tcatttgggc aacatcatcg gctgcgtttt gagtgctgat gtctttgctc ggtactgccg tttgaggggc tataacacca tctacatttg tggcacggat gagtacggca ctgccactga aatcaaggcc atgcaagagg gattaagcga gatggagatc tgtaacaagt atcacgccat gcacaaacaa gcgtacgagt ggtttggctg ccaattcgat aagtttggac gtactaccac cgaaagtcaa actcggatcg cgcaagatat cttcaccaag attcatgcaa ggaactacct tgtggagaaa acgctagatc aactgtggtg cgcccattgc caacgttatc tatcggatcg ttacgtggaa ggcacatgtc caagacctga ttgtcgttat gaggacgcaa gaggagatca atgtgacaaa tgtggccgtt gctatgatgc catggagttg gttaacccgc gctgcaagtt ttgctcgaat acgcctgaaa taaagcgaga agagcacctg ttcctggact tgcccaaact cgaagatcct ctttccaagt gggtcgacaa atcaagtaag gaaggtatct ggtccccgaa tgccatcaac atcacgacca agacctggtt ggctggtggc ttgcaacctc gttgtatcac aagagatctt aagtggggta ctcctgtacc attggaaaag tacaagaaca aagtctttta cgtatggttc gacgcgccca ttggatacct ttccatcact gctgaataca ccgagcaatg ggagttgtgg tggaagaatc cggaagagta tgacgtcaag ctctaccaat tcatgggcaa ggacaacgtg cccttccaca cggtaatctt cccggccagt ctcatggcca ccggcgataa ctggaccctc ctcgatagcg tttgcaccac cgagtacctc aactacgagg gagagcaatt ctccaagagc agaggagtgg gcatctttgg agaccacgtc caaaagactg gcatccctcc ctctgtctgg cgatactacc tgctctccaa cagacccgag tcctccgaca gctccttctc ctggtcggat ctggtggcca agaacaacaa cgagctgctg gccaacctgg gcaactttgt gaaccgctgc ctcaagttca ccgccgaccg cttggacaag accgtctctg cctccttcga tccagagagg gacctcctcc ctactgagga caaggagcaa ttcctggaca aggtgaacga gcatttgaga cagtacgtgg aggccctgga agccaccaag ctcaaggatg gcctctttca ggccatgcaa atctccaagc tgggcaacca atacctccag gaccaaaagc catgggtcct tctctccgaa aaccgaaagc gttgcgaggt ggtctgcgcc gtcgctgtga acgtggtgta cctcctggcc aggttgttgg agccctacat accgcatttc tccgagatgg tgctgcacca gctggccctg ccgcacggcc tgatccccga ccgtttcgcg ttcgaagtgc ccgccggcca tgtcatcagg acgcccttgc ccattttcaa ggagctgacg gagggggagg tggccgagat ccgcagcaag attgacgtta acccggtctt cctggagagg ctgaagcaga agcaagcgca aggctcctcc tcctcctctg ctgctaccgc tggttcctcg aagacggccg aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat