BamaA.07054.a

Lanosterol 14-alpha demethylase (CYP51A)

CENTER ID: BamaA.07054.a
ORGANISM: Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamaA.07054.a.B2.GE43476 mature protein 21 487
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVWWTVAAVT VLVLVILVLA FVKYMTYDPP GIPPVVRWKG SRYGATGALM RDPLGFLTEC RKQYGNVFTI DEILFRRVFV LGVQEMDDLY KTKDEVWDFN KGNEFFFRGI LGPETLNPGA DLAFHNERAI KIVNSLNAPK EVFFNHMKEE TKLWAEELIK RGKPFNMYEE IDILVTNINI RCFVGEEIRK SAPKEFKDLY DELEEKGLSF FNILFYWLPF GTPRRVRYIR KQLNDIIQPI VDKRKREWEE EGKQYHDYLN AYVTIPHKDG TPWTRPELLE RTLTIFFAAR VNTLRTFFWT VMAIITRPNV LERLREEIDG LGEDFTLADL KKLEYMENTM RETVRPIFGG LSASRMCMKS MEYGGYTIPK GYYITTSPLV NNMDPEVFKE PERFNPDRWS PEGDIGKDAS FARKVWGFWG TGPHRCKGEI FAKQLVKTCW TLMFRDYDFK WLDQKEEGEM PAANPLIIMP PPNNPVMCTI SRRGSSE
NT Sequence
atggtgtggt ggacggttgc ggcggtgacg gtgctggtgc tcgtcatcct ggtgttggcc tttgtaaagt acatgaccta tgacccgccg ggcatcccgc cggtggtgcg ttggaagggc agtcgctacg gcgccaccgg cgccttgatg cgtgacccgc tgggtttctt gaccgagtgt cgcaaacaat atggcaacgt ctttaccatt gatgaaatct tgtttaggcg agtgtttgtt ttgggagtgc aagagatgga cgacttgtac aaaaccaaag atgaggtttg ggacttcaac aaaggaaatg agttcttctt ccgtggcatt ttgggtcctg agacgctcaa ccccggagcc gacctcgcct tccacaacga gagagcgatc aagatcgtga actcgctcaa cgcccccaaa gaggtcttct tcaaccacat gaaggaagag accaaactgt gggccgagga actaattaaa cggggcaagc cgttcaacat gtatgaggaa atagatattc tggttaccaa tatcaatatt cgttgctttg tgggagaaga gatccgcaaa agtgctccca aagaattcaa agacctatac gatgaattgg aggagaaggg ccttagcttc tttaacatct tgttctactg gcttcccttt ggcactcctc gccgtgtgcg atacattcgt aagcagttga acgacattat tcagccaatc gtcgacaagc ggaaacgtga gtgggaggaa gaaggcaaac aatatcatga ctacctgaat gcctacgtca ccattcctca caaagatgga accccttgga ctcgaccaga attgttggaa agaactttga ccatcttctt tgcggcccga gttaacaccc ttcgaacctt cttctggacg gtcatggcta tcatcactcg tcccaatgtc ttggaacgac ttcgagagga aattgatggc ttgggggagg actttactct tgcagatctc aagaagctgg agtacatgga aaacaccatg agagagactg tgcgtcccat ctttggcggg ctctccgcct ctcgcatgtg catgaagtcg atggagtacg gcggctatac gatccccaag ggctattaca tcaccacatc gccgctggtc aacaacatgg accctgaggt ctttaaggag cccgagcgat ttaatcctga taggtggtct cctgaaggag acattggaaa ggacgcatcc tttgctcgta aggtgtgggg cttctgggga acgggtccgc atcgctgcaa gggagagatc tttgccaaac aactggtgaa gacctgctgg accctaatgt tccgagacta cgacttcaaa tggttggacc agaaggagga aggggagatg ccggccgcca acccgctgat catcatgcct cctcccaaca atcctgtcat gtgtactatt tccaggcgcg gctcttccga gtaa
Details for BamaA.07054.a.B2.GE43476
HARVESTED ON: 11/19/2019
SEQUENCED ON: 2/5/2020
EXPECTED MW: 56kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHMFV KYMTYDPPGI PPVVRWKGSR YGATGALMRD PLGFLTECRK QYGNVFTIDE ILFRRVFVLG VQEMDDLYKT KDEIWDFNKG NEFFFRGILG PETLNPGADL AFHNERAIKI VNSLNAPKEV FFNHMKEETK LWAEELIKRG KPFNMYEEID ILVTNINIRC FVGEEIRKSA PKEFKDLYDE LEEKGLSFFN ILFYWLPFGT PRRVRYIRKQ XNDIIQPVVD KXKREWDEEX KQYHDYLNAY VTIPHKDGTP WTRPELLERT LTIFFAARVN TLRTFFWTVM AIITRPNVLE RLREEIDGLG EDFTLADLKK LEYMENTMRE TVRPIFGGLS ASRMCMKSME YGGYTIPKGY YITTSPLVNN MDPEVFKEPE RFNPDRWSPE GDIGKDAAFA RKVWGFWGTG PHRCKGEIFA KQLVKTCWTL MFRDYDFKWL DQKEEGEMPA ANPLIIMPPP NNPVMCTISR RGSSE
Validated NT Sequence
atggctcacc accaccacca tatgtttgta aagtacatga cctatgaccc gccgggcatc ccgccggtgg tgcgttggaa gggcagtcgc tacggcgcca ccggcgcctt gatgcgtgac ccgctgggtt tcttgaccga gtgtcgcaaa caatatggca acgtctttac cattgatgaa atcttgttta ggcgagtgtt tgttttggga gtgcaagaga tggacgactt gtacaaaacc aaagatgaga tttgggactt caacaaagga aatgagttct tcttccgtgg cattttgggt cctgagacgc tcaaccccgg agccgacctc gccttccaca acgagagagc gatcaagatc gtgaactcgc tcaacgcccc caaagaggtc ttcttcaacc acatgaagga agagaccaaa ctgtgggccg aggaactaat taaacggggc aagccgttca acatgtatga ggaaatagat attctggtta ccaatatcaa tattcgttgc tttgtgggag aagagatccg caaaagtgct cccaaagaat tcaaagacct atacgatgaa ttggaggaga agggccttag cttctttaac atcttgttct actggcttcc ctttggcact cctcgccgtg tgcgatacat tcgtaagcag ktgaacgaca ttattcagcc ngtcgtcgac aagcsgaaac gtgagtggga cgaagaangc aaacaatatc atgactacct gaatgcntac gtcaccattc ctcacaaaga tggaacccct tggactcgac cagaattgtt ggaaagaact ttgaccatct tctttgcggc ccgagttaac acccttcgaa ccttcttctg gacggtcatg gctatcatca ctcgtcccaa tgtcttggaa cgacttcgag aggaaattga tggcttgggg gaggacttta ctcttgcaga tctcaagaag ctggagtaca tggaaaacac catgagagag actgtgcgtc ccatctttgg cgggctctcc gcctctcgca tgtgcatgaa gtcgatggag tacggcggct atacgatccc caagggctat tacatcacca catcgccgct ggtcaacaac atggaccctg aggtctttaa ggagcccgag cgatttaatc ctgataggtg gtctcctgaa ggagacattg gaaaggacgc agcctttgct cgtaaggtgt ggggcttctg gggaacgggt ccgcatcgct gcaagggaga gatctttgcc aaacaactgg tgaagacctg ctggacccta atgttccgag actacgactt caaatggttg gaccagaagg aggaagggga gatgccggcc gccaacccgc tgatcatcat gcctcctccc aacaatcctg tcatgtgtac tatttccagg cgcggctctt ccgagtaa
Expected Protein Sequence
MAHHHHHHFV KYMTYDPPGI PPVVRWKGSR YGATGALMRD PLGFLTECRK QYGNVFTIDE ILFRRVFVLG VQEMDDLYKT KDEVWDFNKG NEFFFRGILG PETLNPGADL AFHNERAIKI VNSLNAPKEV FFNHMKEETK LWAEELIKRG KPFNMYEEID ILVTNINIRC FVGEEIRKSA PKEFKDLYDE LEEKGLSFFN ILFYWLPFGT PRRVRYIRKQ LNDIIQPIVD KRKREWEEEG KQYHDYLNAY VTIPHKDGTP WTRPELLERT LTIFFAARVN TLRTFFWTVM AIITRPNVLE RLREEIDGLG EDFTLADLKK LEYMENTMRE TVRPIFGGLS ASRMCMKSME YGGYTIPKGY YITTSPLVNN MDPEVFKEPE RFNPDRWSPE GDIGKDASFA RKVWGFWGTG PHRCKGEIFA KQLVKTCWTL MFRDYDFKWL DQKEEGEMPA ANPLIIMPPP NNPVMCTISR RGSSE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattttgtaa agtacatgac ctatgacccg ccgggcatcc cgccggtggt gcgttggaag ggcagtcgct acggcgccac cggcgccttg atgcgtgacc cgctgggttt cttgaccgag tgtcgcaaac aatatggcaa cgtctttacc attgatgaaa tcttgtttag gcgagtgttt gttttgggag tgcaagagat ggacgacttg tacaaaacca aagatgaggt ttgggacttc aacaaaggaa atgagttctt cttccgtggc attttgggtc ctgagacgct caaccccgga gccgacctcg ccttccacaa cgagagagcg atcaagatcg tgaactcgct caacgccccc aaagaggtct tcttcaacca catgaaggaa gagaccaaac tgtgggccga ggaactaatt aaacggggca agccgttcaa catgtatgag gaaatagata ttctggttac caatatcaat attcgttgct ttgtgggaga agagatccgc aaaagtgctc ccaaagaatt caaagaccta tacgatgaat tggaggagaa gggccttagc ttctttaaca tcttgttcta ctggcttccc tttggcactc ctcgccgtgt gcgatacatt cgtaagcagt tgaacgacat tattcagcca atcgtcgaca agcggaaacg tgagtgggag gaagaaggca aacaatatca tgactacctg aatgcctacg tcaccattcc tcacaaagat ggaacccctt ggactcgacc agaattgttg gaaagaactt tgaccatctt ctttgcggcc cgagttaaca cccttcgaac cttcttctgg acggtcatgg ctatcatcac tcgtcccaat gtcttggaac gacttcgaga ggaaattgat ggcttggggg aggactttac tcttgcagat ctcaagaagc tggagtacat ggaaaacacc atgagagaga ctgtgcgtcc catctttggc gggctctccg cctctcgcat gtgcatgaag tcgatggagt acggcggcta tacgatcccc aagggctatt acatcaccac atcgccgctg gtcaacaaca tggaccctga ggtctttaag gagcccgagc gatttaatcc tgataggtgg tctcctgaag gagacattgg aaaggacgca tcctttgctc gtaaggtgtg gggcttctgg ggaacgggtc cgcatcgctg caagggagag atctttgcca aacaactggt gaagacctgc tggaccctaa tgttccgaga ctacgacttc aaatggttgg accagaagga ggaaggggag atgccggccg ccaacccgct gatcatcatg cctcctccca acaatcctgt catgtgtact atttccaggc gcggctcttc cgagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat