SSGCID: Target Page

BamaA.01191.a

Bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS)

CENTER ID:	BamaA.01191.a
ORGANISM:	Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BamaA.01191.a.B1.GE43475	full length		1	484

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAGKGKPFSL VVAATKNWGI GLEGKLPWSL PKEMAHFKAL TLTTSDAAKK NAVIMGRKTF ESIPPKFRPL PNRFNIVLSR TLEKKSFPEG VFIVPSFNSA LELLDCDLSE QVESAFVIGG ANVFEEAMQH KRCRSIYLTR IHSPEYQADT FLTPIDENVF VLDTSYEKYG IPQEENGAHY EFLRYVNVHS EEKNAEPHEE QQYLDLVRDI IENGNTREDR TGVGTKSKFG CQMRFSLRND QFPLLTTKRV FWRGVAEELL WFISGSTNAN TLSDKNVNIW KPNGSKEFLA KRGLGHREEG DLGPVYGFQW RHWGAEYTDM HADYKGKGLD QLAACIDLIK RDPTSRRIVM SAWNPSDLDL MALPPCHMFC QFYVANDELS CQLYQRSADM GLGIPFNIAS YALLTRLVAQ VCGLKSGELV HTIGDAHVYL NHIDGLKEQL QRDPLPFPKL HIKQRGQTID DFTFDDLELE GYQCHKTISL PFAV

NT Sequence

atggcaggca aggggaagcc gttcagcctt gtggtggccg ctaccaagaa ttggggtatc gggttggagg gaaagctgcc gtggtcgctg cccaaggaga tggcccattt caaggcgctc acgctgacca ccagcgacgc agccaagaag aatgccgtca tcatggggcg aaagacgttc gagtccatcc cacccaagtt caggcctttg cccaatcgct ttaacatcgt gctcagtcgc acccttgaaa agaagagctt tcccgaagga gttttcattg tcccaagttt caattctgct ttggagcttc tcgattgtga ccttagcgag caggtagagt ctgcgtttgt gattggggga gccaacgtat tcgaggaggc catgcaacat aaacgctgtc gcagcattta tctcacacgc attcactctc ctgaatacca agcggacact ttccttactc ccattgatga aaacgtgttc gtgctggata cctcctatga aaagtacggc attccacagg aagaaaatgg tgcacactat gaattcttgc gctatgttaa tgtacattct gaagagaaaa atgcagaacc tcacgaagag caacaatatc ttgacttagt gagggacatc attgagaacg gaaatacacg agaggacagg actggcgtcg gtaccaagtc caagtttggt tgtcagatgc ggttcagcct tcgcaacgat cagttccctt tgctgaccac aaagagagtc ttttggagag gcgttgcaga ggaacttctc tggtttatca gcggctctac caacgccaac accctttctg acaaaaacgt gaacatttgg aagccaaatg gttcgaagga gttcttggcc aagcgtggtt tgggccatag ggaggaaggc gacctcggtc ctgtctatgg ttttcaatgg cgtcactggg gtgccgaata cacagacatg catgctgact ataaaggcaa aggcttggac cagctggctg cttgcattga tctcatcaaa cgtgatccca caagccgacg catcgtcatg tcggcctgga atccctccga cctggacctc atggcccttc ctccctgcca catgttttgc caattctacg tcgccaacga tgaactttct tgtcagttat accaacgctc agcagatatg ggactgggca ttccgttcaa catagcgagc tatgctttgt tgacaaggtt ggtggcccag gtatgcggcc taaagagtgg agagttggtg cacaccatcg gcgatgctca tgtctacctc aaccacatcg atggcctcaa agaacaactc cagagggatc ctttgccgtt ccccaagctt cacatcaaac aacgtggtca aactattgat gattttacat ttgatgattt ggagctggaa ggctatcagt gccataagac catcagtctt ccgtttgctg tgtaa

Details for BamaA.01191.a.B1.GE43475

HARVESTED ON:	data unavailable
SEQUENCED ON:	2/5/2020
EXPECTED MW:	56kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA GKGKPFSLVV AATKNWGIGL EGKLPWSLPK EMAHFKALTL TTSDAAKKNA VIMGRKTFES IPPKFRPLPN RFNIVLSRTL EKKSFPEGVF IVPSFNSALE LLDCDLSEQV ESAFVIGGAN VFEEAMQHKR CRSIYLTRIH SPEYQADTFL TPIDENVFVL DTSYEKYGIP QEENGAHYEF LRYVNVHSEE KNAEPHEEQQ YLDLVRDIIE NGNTREDRTG VGTKSKFGCQ MRFSLRNDQF PLLTTKRVFW RGVAEELLWF ISGSTNANTL SDKNVNIWKP NGSKEFLAKR GLGHREEGDL GPVYGFQWRH WGAEYTDMHA DYKGKGLDQL AACIDLIKRD PTSRRIVMSA WNPSDLDLMA LPPCHMFCQF YVANDELSCQ LYQRSADMGL GIPFNIASYA LLTRLVAQVC GLKSGELVHT IGDAHVYLNH IDGLKEQLQR DPLPFPKLHI KQRGQTIDDF TFDDLELEGY QCHKTISLPF AV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag gcaaggggaa gccgttcagc cttgtggtgg ccgctaccaa gaattggggt atcgggttgg agggaaagct gccgtggtcg ctgcccaagg agatggccca tttcaaggcg ctcacgctga ccaccagcga cgcagccaag aagaatgccg tcatcatggg gcgaaagacg ttcgagtcca tcccacccaa gttcaggcct ttgcccaatc gctttaacat cgtgctcagt cgcacccttg aaaagaagag ctttcccgaa ggagttttca ttgtcccaag tttcaattct gctttggagc ttctcgattg tgaccttagc gagcaggtag agtctgcgtt tgtgattggg ggagccaacg tattcgagga ggccatgcaa cataaacgct gtcgcagcat ttatctcaca cgcattcact ctcctgaata ccaagcggac actttcctta ctcccattga tgaaaacgtg ttcgtgctgg atacctccta tgaaaagtac ggcattccac aggaagaaaa tggtgcacac tatgaattct tgcgctatgt taatgtacat tctgaagaga aaaatgcaga acctcacgaa gagcaacaat atcttgactt agtgagggac atcattgaga acggaaatac acgagaggac aggactggcg tcggtaccaa gtccaagttt ggttgtcaga tgcggttcag ccttcgcaac gatcagttcc ctttgctgac cacaaagaga gtcttttgga gaggcgttgc agaggaactt ctctggttta tcagcggctc taccaacgcc aacacccttt ctgacaaaaa cgtgaacatt tggaagccaa atggttcgaa ggagttcttg gccaagcgtg gtttgggcca tagggaggaa ggcgacctcg gtcctgtcta tggttttcaa tggcgtcact ggggtgccga atacacagac atgcatgctg actataaagg caaaggcttg gaccagctgg ctgcttgcat tgatctcatc aaacgtgatc ccacaagccg acgcatcgtc atgtcggcct ggaatccctc cgacctggac ctcatggccc ttcctccctg ccacatgttt tgccaattct acgtcgccaa cgatgaactt tcttgtcagt tataccaacg ctcagcagat atgggactgg gcattccgtt caacatagcg agctatgctt tgttgacaag gttggtggcc caggtatgcg gcctaaagag tggagagttg gtgcacacca tcggcgatgc tcatgtctac ctcaaccaca tcgatggcct caaagaacaa ctccagaggg atcctttgcc gttccccaag cttcacatca aacaacgtgg tcaaactatt gatgatttta catttgatga tttggagctg gaaggctatc agtgccataa gaccatcagt cttccgtttg ctgtgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat