BamaA.01098.a

Histone Deacetylase 1 (HDAC1)

CENTER ID: BamaA.01098.a
ORGANISM: Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BamaA.01098.a.B1.GE43474 full length 1 462
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKQRVCYFY DSDVGNYYYG TGHPMKPHRI RMTHNLLLNY GLYKKMQIYR PRKATKVEMT SFHSEDYVKF LRLITPDNMN DHAKQLQRFN VGEDCPVFDG MYQFCQISAG GSIGGAIKLN HGEADIAINW AGGLHHAKKS EASGFCYIND IVLGILELLK YHSRVLYIDI DIHHGDGVEE AFYTTDRVMT VSFHKFGEYF PGTGDIRDIG AGKGKNYSIN FPLKDGIDDQ SYNDIFKPII QKVMDWYQPG AVVLQCGADS LSGDRLGCFN LSLKGHGECV EFVQSFGVPL LVLGGGGYTI RNVARCWSYE TSLLLKSDIS DELPFNDYLE YYGPDFRLHI TPSNMENLND PKYLEKMKIR LIDNLRNIQG APGVAMTEVP PDTYFESDDE DDENPDERNS ELDRDRRIAH EAELSDSDDE DDRKDFDTHY EDEELGSSTR SSRYSRRGGG VSIMGPVTIE TL
NT Sequence
atgagcaagc agcgcgtctg ttacttttat gatagcgatg tgggcaacta ctactacggc acggggcatc ccatgaagcc gcaccgcata aggatgaccc acaacttgtt gcttaactat ggcctctaca agaaaatgca gatctaccga ccacgcaagg cgacaaaagt ggagatgaca agttttcact ctgaagatta tgtaaagttt ttgcgcctca tcactccgga caacatgaac gatcacgcca agcaattgca aagatttaac gtaggagagg attgtccggt ctttgatggc atgtaccagt tttgtcagat ctcagccggc ggctccatag gcggtgccat aaagctgaac cacggagagg ctgacatagc catcaactgg gctggtggtc tccaccacgc caaaaagtcg gaggcctctg gcttttgcta catcaacgac attgtgctgg gaatcttgga gctgctcaag taccactccc gtgtgttgta catagacatt gatatccatc atggagacgg cgtggaggag gccttctaca cgactgaccg cgtcatgacg gtctccttcc acaaattcgg ggaatatttc ccaggcactg gcgacattag agacatagga gctggtaaag gcaagaacta ctctatcaac ttcccattga aagacggaat agacgatcag agctacaacg acatattcaa gccgatcatt cagaaggtga tggactggta ccaacctggc gctgtggtcc tccaatgcgg cgctgactcc ctgtctggtg ataggcttgg ctgcttcaac ctatctctca agggccacgg cgaatgcgtt gagtttgtac agagctttgg agtacctttg ttggtgcttg gtggtggtgg ttacactatc cgcaacgttg ctcgctgttg gtcctacgaa acctctttgc tgctgaagtc tgacatctcc gatgagcttc cgtttaacga ttatttggaa tactacggac ccgactttcg tcttcacatc acaccttcca acatggagaa cctcaacgac cccaagtatc tggaaaagat gaagattcgc ctcatcgaca acctgcgaaa catacaagga gcacctggcg tggccatgac ggaggtacct cctgatacct attttgagtc agacgatgag gatgacgaga acccagacga aagaaatagt gaactggacc gtgaccgaag aatagcacat gaggcagagc tctccgactc tgatgatgag gacgaccgta aagactttga cactcactac gaggatgagg agctgggctc ttccacgcga tccagcagat actctcgccg gggtggtggc gtttccatca tgggacccgt caccatcgag actttgtag
Details for BamaA.01098.a.B1.GE43474
HARVESTED ON: 11/19/2019
SEQUENCED ON: 2/5/2020
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS KQRVCYFYDS DVGNYYYGTG HPMKPHRIRM THNLLLNYGL YKKMQIYRPR KATKVEMTSF HSEDYVKFLR LITPDNMNDH AKQLQRFNVG EDCPVFDGMY QFCQISAGGS IGGAIKLNHG EADIAINWAG GLHHAKKSEA SGFCYINDIV LGILELLKYH SRVLYIDIDI HHGDGVEEAF YTTDRVMTVS FPQIREYFPG TGDIRDIGAG KGKNYSINFP LKDGIDDQSY NDIFKPIIQK VMDWYQPGAV VLQCGADSLS GDRLGCFNLS LKGHGECVEF VQSFGVPLLV LGGGGYTIRN VARCWSYETS LLLKSDISDE LPFNDYLEYY GPDFRLHITP SNMENLNDPK YLEKMKIRLI DNLRNIQGAP GVAMTEVPPD TYFESDDEDD ENPDERNSEL DRDRRIAHEA ELSDSDDEDD RKDFDTHYED EELGSSTRSS RYSRRGGGVS IMGPVTIETL
Validated NT Sequence
cccatgatgg aaacgccacc accccggcga gagtatctgc tggatcgcgt ggaagagccc agctcctcat cctcgtagtg agtgtcaaag tctttacggt cgtcctcatc atcagagtcg gagagctctg cctcatgtgc tattcttcgg tcacggtcca gttcactatt tctttcgtct gggttctcgt catcctcatc gtctgactca aaataggtat caggaggtac ctccgtcatg gccacgccag gtgctccttg tatgtttcgc aggttgtcga tgaggcgaat cttcatcttt tccagatact tggggtcgtt gaggttctcc atgttggaag gtgtgatgtg aagacgaaag tcgggtccgt agtattccaa ataatcgtta aatggaagct catcggagat gtcagacttc agcagcaaag aggtttcgta ggaccaacag cgagcaacgt tgcggatagt gtaaccacca ccaccaagca ccaacaaagg tactccaaag ctctgtacaa actcaacgca ttcgccgtgg cccttgagag ataggttgaa gcagccaagc ctatcaccag acagggagtc agcgccgcat tggagganca cagcgccagg ttggtaccag tccatcacct tctgaatgat cggcttgaat atgtcgttgt agctctgatc gtctattccg tctttcaatg ggaagttgat agagtagttc ttgcctttac cagctcctat gtctctaatg tcgccagtgc ctgggaaaat attccccgaa tttgtggaaa ggagaccgtc atgacgcggt cagtcgtgta gaagncctcc tccacgccgt ctccatgatg gatatcaatg tctatgtaca acacacggga gtggtacttg agcagctcca agattcccag cacaatgtcg ttgatgtagc aaaagccaga ggcctccgac tttttggcgt ggtggagacc accagcccag ttgatggcnn nntcagcctc tccgtggttc agctttatgg caccgcctat ggagccgccg gctgagatct gacaaaactg gtacanncca tcaaagaccg gacaatcctc tcctacgtta aatctttgca attgcttggc nnnntnnttc atgttgtccg gagtgatgag gcgcaaaaac tttacataat cttcagagtg aaaacttgtc atctccactt ttgtcgcctt gcgtggtcgg tagatctgca ttttcttgta gaggccatag ttaagcaaca agttgtgggt catccttatg cggtgcggct tcatgggatg ccccgtgccg tagtagtagt tgcccacatc gctatcataa aagtaacaga cgcgctgctt gctcatatgg tggtggtggt ggtgagcca
Expected Protein Sequence
MAHHHHHHMS KQRVCYFYDS DVGNYYYGTG HPMKPHRIRM THNLLLNYGL YKKMQIYRPR KATKVEMTSF HSEDYVKFLR LITPDNMNDH AKQLQRFNVG EDCPVFDGMY QFCQISAGGS IGGAIKLNHG EADIAINWAG GLHHAKKSEA SGFCYINDIV LGILELLKYH SRVLYIDIDI HHGDGVEEAF YTTDRVMTVS FHKFGEYFPG TGDIRDIGAG KGKNYSINFP LKDGIDDQSY NDIFKPIIQK VMDWYQPGAV VLQCGADSLS GDRLGCFNLS LKGHGECVEF VQSFGVPLLV LGGGGYTIRN VARCWSYETS LLLKSDISDE LPFNDYLEYY GPDFRLHITP SNMENLNDPK YLEKMKIRLI DNLRNIQGAP GVAMTEVPPD TYFESDDEDD ENPDERNSEL DRDRRIAHEA ELSDSDDEDD RKDFDTHYED EELGSSTRSS RYSRRGGGVS IMGPVTIETL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca agcagcgcgt ctgttacttt tatgatagcg atgtgggcaa ctactactac ggcacggggc atcccatgaa gccgcaccgc ataaggatga cccacaactt gttgcttaac tatggcctct acaagaaaat gcagatctac cgaccacgca aggcgacaaa agtggagatg acaagttttc actctgaaga ttatgtaaag tttttgcgcc tcatcactcc ggacaacatg aacgatcacg ccaagcaatt gcaaagattt aacgtaggag aggattgtcc ggtctttgat ggcatgtacc agttttgtca gatctcagcc ggcggctcca taggcggtgc cataaagctg aaccacggag aggctgacat agccatcaac tgggctggtg gtctccacca cgccaaaaag tcggaggcct ctggcttttg ctacatcaac gacattgtgc tgggaatctt ggagctgctc aagtaccact cccgtgtgtt gtacatagac attgatatcc atcatggaga cggcgtggag gaggccttct acacgactga ccgcgtcatg acggtctcct tccacaaatt cggggaatat ttcccaggca ctggcgacat tagagacata ggagctggta aaggcaagaa ctactctatc aacttcccat tgaaagacgg aatagacgat cagagctaca acgacatatt caagccgatc attcagaagg tgatggactg gtaccaacct ggcgctgtgg tcctccaatg cggcgctgac tccctgtctg gtgataggct tggctgcttc aacctatctc tcaagggcca cggcgaatgc gttgagtttg tacagagctt tggagtacct ttgttggtgc ttggtggtgg tggttacact atccgcaacg ttgctcgctg ttggtcctac gaaacctctt tgctgctgaa gtctgacatc tccgatgagc ttccgtttaa cgattatttg gaatactacg gacccgactt tcgtcttcac atcacacctt ccaacatgga gaacctcaac gaccccaagt atctggaaaa gatgaagatt cgcctcatcg acaacctgcg aaacatacaa ggagcacctg gcgtggccat gacggaggta cctcctgata cctattttga gtcagacgat gaggatgacg agaacccaga cgaaagaaat agtgaactgg accgtgaccg aagaatagca catgaggcag agctctccga ctctgatgat gaggacgacc gtaaagactt tgacactcac tacgaggatg aggagctggg ctcttccacg cgatccagca gatactctcg ccggggtggt ggcgtttcca tcatgggacc cgtcaccatc gagactttgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat