SSGCID: Target Page

BamaA.00032.a

S-adenosyl-L-homocysteine hydrolase

CENTER ID:	BamaA.00032.a
ORGANISM:	Balamuthia mandrillaris CDC:V039; baboon/San Diego/1986
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BamaA.00032.a.B1.GE42936	full length		1	478

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BamaA.00032.a.B1.PW38625	full length		1	478

External Resources

RESOURCE	REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVTQTLVLK GNDGADFKVA DMTLAEFGRR EIELAEIEMP GLMATREKYG REQPLKGVRV AGSLHMTVQT AVLIETLKAL GAEVRWCSCN IYSTQDHAAA AIAATGTPVF AWKGETLEEY WDCTLSALLW SEDQGPQLIV DDGGDATLLI HRGYAAEDNP SILDDDEDNE ELAIVNKLLK RVMEKNPRLW HTIVPQIKGV SEETTTGVKR LYEMMKEGKL LFPALNVNDS VTKSKFDNIY GCRHSLVDAI MRATDVMISG KVAVVCGFGD VGKGSAESLR AQGARVVVTE IDPICALQAL MAGYDVATVE DVVQKADIFV TATGNKDIIR VEHMAKMKHN AIVCNIGHFD NEIQVNALKK VNGIKKIEIK PQVNQYVFPD GHAIILLAEG RLVNLGCATG HPSFVMSNSF TNQTLAQISL WKENYEVGVY TLPKHLDEEV ARLHLEKLGA KLTVLSDEQS KYIGVPKSGP FKPDSYRY

NT Sequence

atgtccgtca ctcaaactct ggtgttgaaa ggcaatgatg gcgctgactt caaggtagcc gacatgaccc tggctgaatt tggcaggagg gaaatcgaac tggccgagat tgagatgccc ggtttgatgg ctacccgcga gaaatacggc agggagcaac ccttgaaggg tgttcgcgtg gccggttctc ttcacatgac cgtgcaaacc gctgtgctga tcgagaccct taaggccttg ggcgctgagg tccgctggtg cagctgcaac atctacagca cccaggacca cgctgctgct gccatcgccg ccaccggcac ccccgtcttt gcctggaagg gtgagacctt ggaggagtac tgggactgca ccctcagcgc tctgctctgg tccgaagatc agggacccca gctcatcgtt gacgacggag gtgacgccac tctcctcatc caccgtggct atgccgctga ggacaaccct tccatcttgg atgacgatga agataacgaa gagctcgcca tcgtaaacaa gttgttgaag cgtgtgatgg agaagaaccc ccgcctgtgg cacaccatcg ttcctcagat taagggagtt tctgaggaga ccaccactgg tgtcaagcgt ctttacgaga tgatgaagga gggcaagctg ctcttccccg ctctgaacgt taacgacagc gtgaccaagt ccaagtttga caacatctat ggttgccgcc atagcttggt ggacgccatc atgcgtgcaa ctgacgtgat gatttccgga aaggttgccg tcgtctgcgg ctttggtgat gtgggcaaag gctccgctga gtccctgaga gctcaaggcg cccgcgttgt tgtcactgaa atcgatccca tctgtgcgtt gcaggccttg atggccggct acgacgtggc caccgtcgag gatgttgttc aaaaggctga catctttgtg accgccactg gtaacaagga catcatccga gtggagcaca tggccaagat gaagcacaac gccatcgtct gcaacattgg acactttgac aatgagattc aggtcaatgc cctcaaaaag gttaacggca tcaagaagat tgagatcaag cctcaggtta accaatatgt cttccctgac ggtcacgcca tcattcttct tgctgagggc cgtttggtta acttgggttg cgccactggc catccttctt tcgtgatgag caactctttc acgaaccaga ctcttgctca gatttctctc tggaaggaga actacgaggt gggcgtctac accctgccca agcacttgga tgaggaagtg gctcgccttc acttggaaaa gctgggcgcc aagcttaccg tcctctctga cgagcaatcc aagtacatcg gtgttcccaa gagcggtccc ttcaagcctg actcctaccg ctactaa

Details for BamaA.00032.a.B1.GE42936

HARVESTED ON:	3/4/2019
SEQUENCED ON:	2/5/2020
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHMSV TQTLVLKGND GADFKVADMT LAEFGRREIE LAEIEMPGLM ATREKYGREQ PLKGVRVAGS LHMTVQTAVL IETLKALGAE VRWCSCNIYS TQDHAAAAIA ATGTPVFAWK GETLEEYWDC TLSALLWSED QGPQLIVDDG GDATLLIHRG YAAEDNPSIL DDDEDNDELA IVNKLLKRVM EKNPRLWHTI VPQIKGVSXE TTTGVKRLYE MMKEGKLLFP ALNVNDSVTK SKFDNIYGCR HSLVDAIMRA TDVMISGKVA VVCGFGDVGK GSAESLRAQG ARVVVTEIDP ICALQALMTG YDVATVEDVV QKADIFVTAT GNKDIIRVEH MAKMKHNAIV CNIGHFDNEI QVNALKKVNG IKKIEIKPQV NQYVFPDGHA IILLAEGRLV NLGCATGHPS FVMSNSFTNQ TLAQISLWKE NYEVGVYTLP KHLDEEVARL HLEKLGAKLT VLSDEQSKYI GVPKSGPFKP DSYRY

Validated NT Sequence

atggct---c accaccacca ccatatgtcc gtcactcaaa ctctggtgtt gaaaggcaat gatggcgctg acttcaaggt agccgacatg accctggctg aatttggcag gagggaaatc gaactggccg agattgagat gcccggtttg atggctaccc gcgagaaata cggcagggag caacccttga agggtgttcg cgtagccggt tctcttcaca tgaccgtgca aaccgctgtg ctgatcgaga cccttaaggc cttgggcgct gaggtccgct ggtgcagctg caacatctac agcacccagg accacgctgc tgctgccatc gccgccaccg gcacccccgt ctttgcctgg aagggtgaaa ccttggagga gtactgggac tgcaccctca gcgctctgct ctggtccgaa gatcagggac cccagctcat cgttgacgac ggaggtgacg ccactctcct catccaccgt ggctatgccg ctgaggacaa cccttccatc ttggatgacg atgaagataa cgatgagctc gccatcgtaa acaagttgtt gaagcgtgtg atggagaaga acccccgcct gtggcacacc atcgttcctc agattaaggg agtttctgan gagactacca cgggtgtcaa gcgtctttac gagatgatga aggagggcaa gctgctcttc cccgctctga acgttaacga cagcgtgacc aagtccaagt ttgacaacat ctatggctgc cgccatagct tggtggacgc catcatgcgt gcaactgacg tgatgatttc cggaaaggtt gccgtagtct gcggctttgg tgatgtgggc aaaggctccg ctgagtccct gagagct-ca aggcgcccgc gttgttgtca ctgaaatcga tcccatctgt gcgttgcagg ccttgatgac cggctacgac gtggccaccg tcgaggatgt tgttcaaaag gctgacatct ttgtgaccgc cactggtaat aaggacatca tccgagtgga gcacatggcc aagatgaagc acaacgccat cgtctgcaac attggacact ttgacaatga gattcaggtc aatgccctca aaaaggttaa cggcatcaag aagattgaga tcaagcctca ggttaaccaa tatgtcttcc ctgacggtca cgccatcatt cttcttgctg agggccgttt ggttaacttg ggctgtgcca ctggtcatcc ttctttcgtg atgagcaact ctttcacgaa ccagactctt gctcagattt ctctctggaa ggagaactac gaggtgggcg tctacaccct gcccaagcac ttggatgagg aagtggctcg ccttcacttg gaaaagctgg gcgccaagct taccgtcctc tctgacgagc aatccaagta catcggtgtt cccaagagcg gtcccttcaa gcctgactcc taccgctact aa

Expected Protein Sequence

MAHHHHHHMS VTQTLVLKGN DGADFKVADM TLAEFGRREI ELAEIEMPGL MATREKYGRE QPLKGVRVAG SLHMTVQTAV LIETLKALGA EVRWCSCNIY STQDHAAAAI AATGTPVFAW KGETLEEYWD CTLSALLWSE DQGPQLIVDD GGDATLLIHR GYAAEDNPSI LDDDEDNEEL AIVNKLLKRV MEKNPRLWHT IVPQIKGVSE ETTTGVKRLY EMMKEGKLLF PALNVNDSVT KSKFDNIYGC RHSLVDAIMR ATDVMISGKV AVVCGFGDVG KGSAESLRAQ GARVVVTEID PICALQALMA GYDVATVEDV VQKADIFVTA TGNKDIIRVE HMAKMKHNAI VCNIGHFDNE IQVNALKKVN GIKKIEIKPQ VNQYVFPDGH AIILLAEGRL VNLGCATGHP SFVMSNSFTN QTLAQISLWK ENYEVGVYTL PKHLDEEVAR LHLEKLGAKL TVLSDEQSKY IGVPKSGPFK PDSYRY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg tcactcaaac tctggtgttg aaaggcaatg atggcgctga cttcaaggta gccgacatga ccctggctga atttggcagg agggaaatcg aactggccga gattgagatg cccggtttga tggctacccg cgagaaatac ggcagggagc aacccttgaa gggtgttcgc gtggccggtt ctcttcacat gaccgtgcaa accgctgtgc tgatcgagac ccttaaggcc ttgggcgctg aggtccgctg gtgcagctgc aacatctaca gcacccagga ccacgctgct gctgccatcg ccgccaccgg cacccccgtc tttgcctgga agggtgagac cttggaggag tactgggact gcaccctcag cgctctgctc tggtccgaag atcagggacc ccagctcatc gttgacgacg gaggtgacgc cactctcctc atccaccgtg gctatgccgc tgaggacaac ccttccatct tggatgacga tgaagataac gaagagctcg ccatcgtaaa caagttgttg aagcgtgtga tggagaagaa cccccgcctg tggcacacca tcgttcctca gattaaggga gtttctgagg agaccaccac tggtgtcaag cgtctttacg agatgatgaa ggagggcaag ctgctcttcc ccgctctgaa cgttaacgac agcgtgacca agtccaagtt tgacaacatc tatggttgcc gccatagctt ggtggacgcc atcatgcgtg caactgacgt gatgatttcc ggaaaggttg ccgtcgtctg cggctttggt gatgtgggca aaggctccgc tgagtccctg agagctcaag gcgcccgcgt tgttgtcact gaaatcgatc ccatctgtgc gttgcaggcc ttgatggccg gctacgacgt ggccaccgtc gaggatgttg ttcaaaaggc tgacatcttt gtgaccgcca ctggtaacaa ggacatcatc cgagtggagc acatggccaa gatgaagcac aacgccatcg tctgcaacat tggacacttt gacaatgaga ttcaggtcaa tgccctcaaa aaggttaacg gcatcaagaa gattgagatc aagcctcagg ttaaccaata tgtcttccct gacggtcacg ccatcattct tcttgctgag ggccgtttgg ttaacttggg ttgcgccact ggccatcctt ctttcgtgat gagcaactct ttcacgaacc agactcttgc tcagatttct ctctggaagg agaactacga ggtgggcgtc tacaccctgc ccaagcactt ggatgaggaa gtggctcgcc ttcacttgga aaagctgggc gccaagctta ccgtcctctc tgacgagcaa tccaagtaca tcggtgttcc caagagcggt cccttcaagc ctgactccta ccgctactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BamaA.00032.a.B1.PW38625

PURIFICATION DATe:	6/4/2019
CONCENTRATION:	25mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	14
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG TCACTCAAAC TCTGGTGTTG AAAGGCAATG ATGGCGCTGA CTTCAAGGTA GCCGACATGA CCCTGGCTGA ATTTGGCAGG AGGGAAATCG AACTGGCCGA GATTGAGATG CCCGGTTTGA TGGCTACCCG CGAGAAATAC GGCAGGGAGC AACCCTTGAA GGGTGTTCGC GTGGCCGGTT CTCTTCACAT GACCGTGCAA ACCGCTGTGC TGATCGAGAC CCTTAAGGCC TTGGGCGCTG AGGTCCGCTG GTGCAGCTGC AACATCTACA GCACCCAGGA CCACGCTGCT GCTGCCATCG CCGCCACCGG CACCCCCGTC TTTGCCTGGA AGGGTGAGAC CTTGGAGGAG TACTGGGACT GCACCCTCAG CGCTCTGCTC TGGTCCGAAG ATCAGGGACC CCAGCTCATC GTTGACGACG GAGGTGACGC CACTCTCCTC ATCCACCGTG GCTATGCCGC TGAGGACAAC CCTTCCATCT TGGATGACGA TGAAGATAAC GAAGAGCTCG CCATCGTAAA CAAGTTGTTG AAGCGTGTGA TGGAGAAGAA CCCCCGCCTG TGGCACACCA TCGTTCCTCA GATTAAGGGA GTTTCTGAGG AGACCACCAC TGGTGTCAAG CGTCTTTACG AGATGATGAA GGAGGGCAAG CTGCTCTTCC CCGCTCTGAA CGTTAACGAC AGCGTGACCA AGTCCAAGTT TGACAACATC TATGGTTGCC GCCATAGCTT GGTGGACGCC ATCATGCGTG CAACTGACGT GATGATTTCC GGAAAGGTTG CCGTCGTCTG CGGCTTTGGT GATGTGGGCA AAGGCTCCGC TGAGTCCCTG AGAGCTCAAG GCGCCCGCGT TGTTGTCACT GAAATCGATC CCATCTGTGC GTTGCAGGCC TTGATGGCCG GCTACGACGT GGCCACCGTC GAGGATGTTG TTCAAAAGGC TGACATCTTT GTGACCGCCA CTGGTAACAA GGACATCATC CGAGTGGAGC ACATGGCCAA GATGAAGCAC AACGCCATCG TCTGCAACAT TGGACACTTT GACAATGAGA TTCAGGTCAA TGCCCTCAAA AAGGTTAACG GCATCAAGAA GATTGAGATC AAGCCTCAGG TTAACCAATA TGTCTTCCCT GACGGTCACG CCATCATTCT TCTTGCTGAG GGCCGTTTGG TTAACTTGGG TTGCGCCACT GGCCATCCTT CTTTCGTGAT GAGCAACTCT TTCACGAACC AGACTCTTGC TCAGATTTCT CTCTGGAAGG AGAACTACGA GGTGGGCGTC TACACCCTGC CCAAGCACTT GGATGAGGAA GTGGCTCGCC TTCACTTGGA AAAGCTGGGC GCCAAGCTTA CCGTCCTCTC TGACGAGCAA TCCAAGTACA TCGGTGTTCC CAAGAGCGGT CCCTTCAAGC CTGACTCCTA CCGCTACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT