SSGCID: Target Page

BaheA.18608.a

Hemin binding protein d

CENTER ID:	BaheA.18608.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.18608.a.B1.GE37165	full length		1	274

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.521
RefSeq:	YP_033317.1
UniProt:	A0A0H3M554

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTKYLITTS IFSLISISIA QAADVIVPEQ PVPVVAVPAF SWAGFYLGGQ VGNFSSKTAL SYLGDEKTGK WTPVKKEFVP KLSGFVGGIY AGSNFDINNG FIIGIDTDVM WSGKKNTKII TWPEVPPEVS SEKKVERVSV GTNQDTSAEV TNGALSHTLK QKWFGATRVK VGFVVDRMMP YIAGGVAYTQ LQNIFEKEAD KTKKAIDISN VIHDEKKTMV GYTLGGGIDF AMTDNVIVRA EYRYSDYGKK KFADEKIEMR YKTNDFRVGV AYKF

NT Sequence

atgactacaa aatatttaat cacaacatct attttttctt taatttcaat ttctatagcg caggcagcgg atgttatagt tccagaacaa ccagtgcctg tggtggcagt tcctgctttt tcctgggcag gattttacct tggtggccaa gttggtaatt tttcaagtaa gactgctttg agttatttag gagatgagaa aacagggaaa tggactccgg ttaaaaagga atttgtaccc aaactttctg gttttgtagg tgggatttat gcaggttcca actttgatat caataatggt tttattatag gtattgatac ggatgtgatg tggtctggta aaaaaaatac gaaaattatt acttggccag aagttccgcc agaagtctcg tcagaaaaaa aagtggaaag agtttcagta ggaactaatc aagacacttc tgcagaagtg acgaatggag ctttgtctca tactttaaaa caaaaatggt ttggtgccac acgagtgaaa gttggttttg ttgttgatcg tatgatgcct tatattgcag gaggtgttgc ttatacgcag cttcaaaata tctttgaaaa ggaagctgat aaaacaaaga aagccattga tatttctaat gttattcatg atgaaaaaaa gaccatggtt ggttataccc ttggaggagg cattgatttt gcaatgactg ataatgttat tgtacgtgca gaatatcgtt attcagatta tggtaagaag aagttcgcag atgaaaaaat tgaaatgcgc tacaaaacaa atgattttcg tgttggtgta gcgtacaagt tttga

Details for BaheA.18608.a.B1.GE37165

HARVESTED ON:	8/13/2013
SEQUENCED ON:	8/15/2013
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT TKYLITTSIF SLISISIAQA ADVIVPEQPV PVVAVPAFSW AGFYLGGQVG NFSSKTALSY LGDEKTGKWT PVKKEFVPKL SGFVGGIYAG SNFDINNGFI IGIDTDVMWS GKKNTKIITW PEVPPEVSSE KKVERVSVGT NQDTSAEVTN GALSHTLKQK WFGATRVKVG FVVDRMMPYI AGGVAYTQLQ NIFEKEADKT KKAIDISNVI HDEKKTMVGY TLGGGIDFAM TDNVIVRAEY RYSDYGKKKF ADEKIEMRYK TNDFRVGVAY KF

Validated NT Sequence

attttgntnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatgact acaaaatatt taatcacaac atctattttt tctttaattt caatttctat agcgcaggca gcggatgtta tagttccaga acaaccagtg cctgtggtgg cagttcctgc tttttcctgg gcaggatttt accttggtgg ccaagttggt aatttttcaa gtaagactgc tttgagttat ttaggagatg agaaaacagg gaaatggact ccggttaaaa aggaatttgt acccaaactt tctggttttg taggtgggat ttatgcaggt tccaactttg atatcaataa tggttttatt ataggtattg atacggatgt gatgtggtct ggtaaaaaaa atacgaaaat tattacttgg ccagaagttc cgccagaagt ctcgtcagaa aaaaaagtgg aaagagtttc agtaggaact aatcaagaca cttctgcaga agtgacgaat ggagctttgt ctcatacttt aaaacaaaaa tggtttggtg ccacacgagt gaaagttggt tttgttgttg atcgtatgat gccttatatt gcaggaggtg ttgcttatac gcagcttcaa aatatctttg aaaaggaagc tgataaaaca aagaaagcca ttgatatttc taatgttatt catgatgaaa aaaagaccat ggttggttat acccttggag gaggcattga ttttgcaatg actgataatg ttattgtacg tgcagaatat cgttattcag attatggtaa gaagaagttc gcagatgaaa aaattgaaat gcgctacaaa acaaatgatt ttcgtgttgg tgtagcgtac aagttttaag tgagtaagan aggatccggn nnnnnnnnnn cccc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta caaaatattt aatcacaaca tctatttttt ctttaatttc aatttctata gcgcaggcag cggatgttat agttccagaa caaccagtgc ctgtggtggc agttcctgct ttttcctggg caggatttta ccttggtggc caagttggta atttttcaag taagactgct ttgagttatt taggagatga gaaaacaggg aaatggactc cggttaaaaa ggaatttgta cccaaacttt ctggttttgt aggtgggatt tatgcaggtt ccaactttga tatcaataat ggttttatta taggtattga tacggatgtg atgtggtctg gtaaaaaaaa tacgaaaatt attacttggc cagaagttcc gccagaagtc tcgtcagaaa aaaaagtgga aagagtttca gtaggaacta atcaagacac ttctgcagaa gtgacgaatg gagctttgtc tcatacttta aaacaaaaat ggtttggtgc cacacgagtg aaagttggtt ttgttgttga tcgtatgatg ccttatattg caggaggtgt tgcttatacg cagcttcaaa atatctttga aaaggaagct gataaaacaa agaaagccat tgatatttct aatgttattc atgatgaaaa aaagaccatg gttggttata cccttggagg aggcattgat tttgcaatga ctgataatgt tattgtacgt gcagaatatc gttattcaga ttatggtaag aagaagttcg cagatgaaaa aattgaaatg cgctacaaaa caaatgattt tcgtgttggt gtagcgtaca agttttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat