SSGCID: Target Page

BaheA.18412.a

Type IV secretion system protein virB4 (VirB type IV secretion machinery)

CENTER ID:	BaheA.18412.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.18412.a.B1.GE34966	full length		1	784

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1448
RefSeq:	YP_034053.1
UniProt:	Q9R2W4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSMMKRESLP EDYIPYIRHI NQHVIALNSR CLMTVMVVEG VNFDTADIDQ LNSLHNQLNT LLKNIADERV ALYSHIIRRR ETIYPESQFF SSFAATLDEK YKKKMVSQEL YRNDLFVSLL WNPASDKTEQ LASFFQRLAK AKKTQSEPDQ EAIRKIEELS QDLIEGLESY GARLLSVYAH GGILFSEQSE FLHQLVGGRR ERIPLTFGTI ASTIYSDRVI FGKETIEIRH ESNERFAGMF GWKEYPSKTR PGMTDGLLTA PFEFILTQSF VFKSKAAASV IMGRKQNQMI NAADRASSQI EALDEALDDL ESNRFVLGEH HLSLAVFANH PKALAEYLSK ARAHLTNGGA VIAREDLGLE AAWWAQLPGN FSYRARSGAI TSRNFAALSP FHSFPIGKLE GNVWGTAVAL LKTQAGSPYY FNFHYGDLGN TFVCGPSGSG KTVIVNFLLA QLQKHNPTMV FFDKDQGAEI FVRAGGGKYK PLKNGQPTGI APLKGMEYTE KNKVFLRNWV LKLVTAEGQT VTEEERQDIA KAIDALGNLP HAQRSLSALQ LFFDNTSKEG IAIRLQRWLK GNDLGWVFDN DQDDLNLDSQ FIGYDMTDFL DNEEIRRPLM MYLFNRILDL IDGRRIIIVI DEFWKALEDD SFKAFAQDRL KTIRKQNGMM LFATQSPKDA LNSTIAHTII EQCPTQIFFP NQKANYKDYV EDFKLTEREF ELIQSELSRE SRRFLIKQGQ SSVVAELNLR GMNDEIAVLS GTTKNIELVN QIISEYGADP DIWLPIFHQR RENQ

NT Sequence

atgtcaatga tgaaacggga atctttgcct gaagactata ttccctacat acgccacatc aaccaacatg ttattgcatt aaactcccgc tgcttaatga ctgtgatggt tgttgaaggt gtaaattttg acactgcaga tatcgaccag ttaaattcct tacacaacca gttaaacaca cttttgaaaa atatcgccga tgaacgggtt gctttatatt ctcacatcat ccgtcggcgt gaaacgattt atccagagag tcagtttttt tcctcttttg cagcaacatt agatgaaaaa tacaaaaaga aaatggtttc acaagagttg tatagaaatg atctctttgt ttcactgctt tggaatccgg catcagataa gactgaacag cttgcttcat tttttcagcg cttagcaaaa gcgaagaaaa cacaatctga acccgatcag gaagccattc gtaaaattga agagttaagc caagatctta tagaaggatt ggaaagctat ggagcgcggc ttttgtcagt ctatgcacat ggaggcattt tattttccga acaaagtgaa tttctccacc agttagtggg aggaaggcgt gaacgcattc ctctcacatt tggcactatt gcctcaacga tttactcaga ccgtgtcatt tttggcaaag aaacgattga aattcgccat gaaagcaatg aacgctttgc tggcatgttt ggctggaaag aatatccctc taaaacccga ccagggatga ctgatggttt actcacagca ccgtttgaat ttatcttaac acaatccttt gtctttaaga gtaaagcagc ggctagtgtc attatgggtc gcaaacaaaa tcaaatgata aatgcggccg atcgtgccag ctcacaaatt gaagcacttg atgaagcact tgatgattta gaatcaaatc gttttgtttt gggcgaacat catctttctc tagccgtttt tgcgaaccac ccaaaggcct tggctgaata tctctcaaaa gcacgtgctc acttaaccaa tggaggagcc gttatcgcga gagaagattt aggattggaa gcagcatggt gggcgcaact tcctggcaat ttcagttatc gtgcgcgatc aggagccatt accagcagaa attttgcagc tttatctccc tttcattcct ttcccattgg taaactggaa ggcaatgtat ggggaactgc tgtggcatta ctaaaaacac aagcgggatc tccttattat tttaattttc attatgggga cttgggcaat acctttgttt gcggtccctc aggatctgga aaaacggtga ttgttaattt tcttcttgca caactccaaa aacacaaccc gacaatggtc tttttcgaca aagatcaggg tgcagaaatt tttgtccgtg ctggcggagg aaaatataag cctttgaaaa atggacaacc tacgggcatt gcccccttaa agggcatgga atacacagaa aaaaacaaag tttttcttcg taattgggtc ttaaagctgg tgacagctga aggacaaacg gtgacagaag aagaacgaca agatattgcc aaagccattg atgccttggg aaatctcccc catgcacaac gttctcttag tgccctccaa ttgttttttg ataacacatc aaaagaaggc attgccatac gcttacaacg ctggctcaaa ggcaatgact taggctgggt ttttgacaac gatcaagacg atctcaattt agactctcaa ttcattggtt atgacatgac cgatttctta gacaatgaag aaattcggcg tcccttgatg atgtacctgt ttaaccgaat tctcgatctt attgatgggc gacgtattat tattgtcatt gatgaattct ggaaagcgct tgaagatgat tccttcaaag cgtttgcgca agatcgcctt aaaacgatcc gtaaacaaaa tggtatgatg ctctttgcaa cacaaagccc aaaagatgct ttaaattcaa caattgcaca caccattatt gagcaatgcc ccacccaaat attttttcca aatcaaaaag caaattataa agattatgtt gaagatttca aactgactga gcgagaattt gagctgatac aatcagaatt aagtcgagaa tcccgtcgtt ttctcatcaa acagggacaa agttccgttg ttgcagaact taacttacgt ggaatgaatg atgagatcgc cgtcttaagc ggtacaacaa agaatattga actggtgaac caaattatca gcgaatatgg agcagaccca gacatatggc tgcctatatt tcatcaaagg agagaaaatc aatga

Details for BaheA.18412.a.B1.GE34966

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	90kDa
OBSERVED MW:	90kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	37

Validated AA Sequence

Validated NT Sequence

ttttnnnnnn nttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa tgatgaaacg ggaatctttg cctgaagact atattcccta catacgccac atcaaccaac atgttattgc attaaactcc cgctgcttaa tgactgtgat ggttgttgaa ggtgtaaatt ttgacactgc agatatcgac cagttaaatt ccttacacaa ccagttaaac acacttttga aaaatatcgc cgatgaacgg gttgctttat attctcacat catccgtcgg cgtgaaacga tttatccaga gagtcagttt ttttcctctt ttgcagcaac attagatgaa aaatacaaaa agaaaatggt ttcacaagag ttgtatagaa atgatctctt tgtttcactg ctttggaatc cggcatcaga taagactgaa cagcttgctt cattttttca gcgcttagca aaagcgaaga aaacacaatc tgaacccgat caggaagcca ttcgtaaaat tgaagagtta agccaagatc ttatagaagg attggaaagc tatggagcgc ggcttttgtc agtctatgca catggaggca ttttattttc cgaacaaagt gaatttctcc accagttagt gggaggaagg cgtgaacgca ttcctctcac atttggcact attgcctcaa cgatttactc agaccgtgtc atttttggca aagaaacgat tgaaattcgc catgaaagca atgaacgctt tgctggcatg tttggctgga aagaatatcc ctctaaaacc cgaccaggga tgactgatgg tttactcaca gcaccgtttg aatttatctt aacacaatcc tttgtcttta agagtaaagc agcggctagt gtcattatgg gtcgcnncaa aatcaaatga taaatgcggc cgatcgtgcc agctcacnaa ntgaagcact tgatgaagca cttgannatt taatcnaatc nttnntttng gnnaacatca nnnntnnnnt anncnntttn annnncnnnn ggncnnggnn nnannannnn nnnnnnannn nnnnngnctt nnnnnnnnnn ngnnnnnnnn tnnnncnnnn nnnnnnnnnn nnannnnnnn nnnngnnngn nnnnnnnntn nnnnnnnnnn nnnnnncnnn nnnnnnnnnn nnnnntnnnn nnnnnnnnnn gnnnngng

Expected Protein Sequence

MAHHHHHHMS MMKRESLPED YIPYIRHINQ HVIALNSRCL MTVMVVEGVN FDTADIDQLN SLHNQLNTLL KNIADERVAL YSHIIRRRET IYPESQFFSS FAATLDEKYK KKMVSQELYR NDLFVSLLWN PASDKTEQLA SFFQRLAKAK KTQSEPDQEA IRKIEELSQD LIEGLESYGA RLLSVYAHGG ILFSEQSEFL HQLVGGRRER IPLTFGTIAS TIYSDRVIFG KETIEIRHES NERFAGMFGW KEYPSKTRPG MTDGLLTAPF EFILTQSFVF KSKAAASVIM GRKQNQMINA ADRASSQIEA LDEALDDLES NRFVLGEHHL SLAVFANHPK ALAEYLSKAR AHLTNGGAVI AREDLGLEAA WWAQLPGNFS YRARSGAITS RNFAALSPFH SFPIGKLEGN VWGTAVALLK TQAGSPYYFN FHYGDLGNTF VCGPSGSGKT VIVNFLLAQL QKHNPTMVFF DKDQGAEIFV RAGGGKYKPL KNGQPTGIAP LKGMEYTEKN KVFLRNWVLK LVTAEGQTVT EEERQDIAKA IDALGNLPHA QRSLSALQLF FDNTSKEGIA IRLQRWLKGN DLGWVFDNDQ DDLNLDSQFI GYDMTDFLDN EEIRRPLMMY LFNRILDLID GRRIIIVIDE FWKALEDDSF KAFAQDRLKT IRKQNGMMLF ATQSPKDALN STIAHTIIEQ CPTQIFFPNQ KANYKDYVED FKLTEREFEL IQSELSRESR RFLIKQGQSS VVAELNLRGM NDEIAVLSGT TKNIELVNQI ISEYGADPDI WLPIFHQRRE NQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa tgatgaaacg ggaatctttg cctgaagact atattcccta catacgccac atcaaccaac atgttattgc attaaactcc cgctgcttaa tgactgtgat ggttgttgaa ggtgtaaatt ttgacactgc agatatcgac cagttaaatt ccttacacaa ccagttaaac acacttttga aaaatatcgc cgatgaacgg gttgctttat attctcacat catccgtcgg cgtgaaacga tttatccaga gagtcagttt ttttcctctt ttgcagcaac attagatgaa aaatacaaaa agaaaatggt ttcacaagag ttgtatagaa atgatctctt tgtttcactg ctttggaatc cggcatcaga taagactgaa cagcttgctt cattttttca gcgcttagca aaagcgaaga aaacacaatc tgaacccgat caggaagcca ttcgtaaaat tgaagagtta agccaagatc ttatagaagg attggaaagc tatggagcgc ggcttttgtc agtctatgca catggaggca ttttattttc cgaacaaagt gaatttctcc accagttagt gggaggaagg cgtgaacgca ttcctctcac atttggcact attgcctcaa cgatttactc agaccgtgtc atttttggca aagaaacgat tgaaattcgc catgaaagca atgaacgctt tgctggcatg tttggctgga aagaatatcc ctctaaaacc cgaccaggga tgactgatgg tttactcaca gcaccgtttg aatttatctt aacacaatcc tttgtcttta agagtaaagc agcggctagt gtcattatgg gtcgcaaaca aaatcaaatg ataaatgcgg ccgatcgtgc cagctcacaa attgaagcac ttgatgaagc acttgatgat ttagaatcaa atcgttttgt tttgggcgaa catcatcttt ctctagccgt ttttgcgaac cacccaaagg ccttggctga atatctctca aaagcacgtg ctcacttaac caatggagga gccgttatcg cgagagaaga tttaggattg gaagcagcat ggtgggcgca acttcctggc aatttcagtt atcgtgcgcg atcaggagcc attaccagca gaaattttgc agctttatct ccctttcatt cctttcccat tggtaaactg gaaggcaatg tatggggaac tgctgtggca ttactaaaaa cacaagcggg atctccttat tattttaatt ttcattatgg ggacttgggc aatacctttg tttgcggtcc ctcaggatct ggaaaaacgg tgattgttaa ttttcttctt gcacaactcc aaaaacacaa cccgacaatg gtctttttcg acaaagatca gggtgcagaa atttttgtcc gtgctggcgg aggaaaatat aagcctttga aaaatggaca acctacgggc attgccccct taaagggcat ggaatacaca gaaaaaaaca aagtttttct tcgtaattgg gtcttaaagc tggtgacagc tgaaggacaa acggtgacag aagaagaacg acaagatatt gccaaagcca ttgatgcctt gggaaatctc ccccatgcac aacgttctct tagtgccctc caattgtttt ttgataacac atcaaaagaa ggcattgcca tacgcttaca acgctggctc aaaggcaatg acttaggctg ggtttttgac aacgatcaag acgatctcaa tttagactct caattcattg gttatgacat gaccgatttc ttagacaatg aagaaattcg gcgtcccttg atgatgtacc tgtttaaccg aattctcgat cttattgatg ggcgacgtat tattattgtc attgatgaat tctggaaagc gcttgaagat gattccttca aagcgtttgc gcaagatcgc cttaaaacga tccgtaaaca aaatggtatg atgctctttg caacacaaag cccaaaagat gctttaaatt caacaattgc acacaccatt attgagcaat gccccaccca aatatttttt ccaaatcaaa aagcaaatta taaagattat gttgaagatt tcaaactgac tgagcgagaa tttgagctga tacaatcaga attaagtcga gaatcccgtc gttttctcat caaacaggga caaagttccg ttgttgcaga acttaactta cgtggaatga atgatgagat cgccgtctta agcggtacaa caaagaatat tgaactggtg aaccaaatta tcagcgaata tggagcagac ccagacatat ggctgcctat atttcatcaa aggagagaaa atcaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat