SSGCID: Target Page

BaheA.18411.a

Type IV secretion system protein virB10 (VirB type IV secretion machinery)

CENTER ID:	BaheA.18411.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.18411.a.B2.GE34963	soluble domain		52	396

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.18411.a.B2.PS01496	soluble domain		52	396

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1454
RefSeq:	YP_034059.1
UniProt:	Q6G2B2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNDPMDENNL LNDRDMIKDG HGKKQRPNTS KAAALVILFG VCLYLAYSTL FTEKQQPVEV QKEGIIKQTE LFRPAPPKPV SLEPTIEKNN VLLPKVELPT PPKKTTNSDD SLLEAAQRAP VLAYANTQKG QGSTEKNKDI SANQPEAKPD ETAQRFNHLL KPTTLEGIRA AKLGNRNYII AMGASIPCIL ETAISSDQQG FASCIVSRDI LSDNGRVVLL DKGTQIVGEY RAGLKKGQKR LFVLWNRAKT PNGIIITLAS PATDALGRSG MDGDIDNHWL ERIGSALLVS IVKDATNYVK GRLPKDQDKN NSETISSGQN IANIAVENYA NIPPTLSKNQ GEMVNVFVAR DLDFSNVYKL KVIENKKQIV NRALSRNFYK NSAVICNEPK LAHIER

NT Sequence

ATGAATGATC CAATGGATGA AAACAACCTG CTAAATGATC GCGATATGAT AAAAGACGGT CACGGAAAAA AGCAACGTCC CAATACAAGT AAAGCGGCTG CTCTTGTTAT TCTTTTTGGT GTTTGTCTTT ATTTAGCGTA TTCAACGCTT TTCACAGAGA AACAACAACC GGTTGAAGTT CAAAAAGAAG GAATTATTAA GCAAACGGAA CTTTTCCGTC CAGCCCCACC CAAACCTGTA TCCCTGGAGC CAACGATAGA AAAAAATAAC GTTCTGTTGC CTAAGGTTGA ACTACCAACT CCCCCCAAAA AAACAACCAA TTCTGATGAT TCACTCTTAG AAGCAGCACA ACGTGCTCCT GTCTTAGCCT ATGCCAACAC ACAAAAAGGC CAAGGGAGCA CAGAAAAAAA CAAAGACATA AGCGCGAACC AACCTGAAGC CAAGCCTGAT GAAACAGCGC AACGCTTTAA TCATCTTCTC AAACCAACAA CCCTTGAAGG CATTCGTGCT GCAAAACTTG GCAATCGAAA TTACATCATC GCAATGGGCG CTTCCATTCC GTGTATCTTA GAAACCGCGA TCAGTAGTGA CCAACAAGGA TTTGCCAGCT GTATTGTCTC ACGAGATATC TTGTCTGATA ATGGCCGCGT TGTTCTTCTT GATAAAGGCA CCCAAATCGT TGGCGAGTAT CGTGCTGGAT TAAAAAAAGG ACAAAAGCGT CTCTTTGTTC TATGGAATAG AGCCAAAACA CCAAATGGTA TCATTATCAC CTTAGCTTCA CCGGCAACAG ATGCCTTAGG ACGTTCTGGT ATGGATGGAG ATATTGATAA CCATTGGTTA GAGAGAATTG GCTCTGCGCT TCTTGTATCG ATTGTCAAAG ATGCAACAAA CTATGTGAAA GGCCGCTTGC CAAAAGACCA AGATAAGAAC AATTCTGAAA CAATTTCTTC AGGACAAAAC ATTGCAAATA TTGCCGTAGA AAATTACGCC AATATTCCCC CTACCCTGTC CAAGAACCAA GGGGAAATGG TCAATGTTTT TGTGGCTCGC GATTTAGATT TTTCCAATGT TTATAAATTG AAAGTGATCG AAAACAAAAA ACAGATTGTC AATCGGGCTC TTTCGAGAAA CTTTTATAAA AATTCTGCGG TAATTTGCAA TGAACCAAAA CTTGCACACA TTGAGCGATG A

Details for BaheA.18411.a.B2.GE34963

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	58

Validated AA Sequence

MGASIPCILE TAISSDQQGF ASCIVSRDIL SDNGRVVLLD KGTQIVGEYR AGLKKGQKRL FVLWNRAKTP NGIIITLASP ATDALGRSGM DGDIDNHWLE RIGSALLVSI VKDATNYVKG RLPKDQDKNN SETISSGQNI ANIAVENYAN IPPTLSKNQG EMVNVFVARD LDFSNVYKLK VIENKKQIVN RALSRNFYKN S

Validated NT Sequence

cagaattttt ataaaagttt ctcgaaagag cccgattgac aatctgtttt ttgttttcga tcactttcaa tttataaaca ttggaaaaat ctaaatcgcg agccacaaaa acattgacca tttccccttg gttcttggac agggtagggg gaatattggc gtaattttct acggcaatat ttgcaatgtt ttgtcctgaa gaaattgttt cagaattgtt cttatcttgg tcttttggca agcggccttt cacatagttt gttgcatctt tgacaatcga tacaagaagc gcagagccaa ttctctctaa ccaatggtta tcaatatctc catccatacc agaacgtcct aaggcatctg ttgccggtga agctaaggtg ataatgatac catttggtgt tttggctcta ttccatagaa caaagagacg cttttgtcct ttttttaatc cagcacgata ctcgccaacg atttgggtgc ctttatcaag aagaacaacg cggccattat cagacaagat atctcgtgag acaatacagc tggcaaatcc ttgttggtca ctactgatcg cggtttctaa gatacacgga atggaagcgc ccattgcgat gatgtaattt cgattgccaa gttttgcagc acgaatgcct tcaagggttg ttggtttgag aagatgatta aagcgttgcg ctgtttcatc aggcttggct tcaggttggt tcgcgcttat gtctttgttt ttttctgtgc tcccttggcc tttttgtgtg ttggcatagg ctaagacagg agcacgttgt gctgcttcta agagtgaatc atcagaattg gttgtttttt tggggggagt tggtagttca accttaggca acagaacgtt atttttttct atcgttggct ccagggatac aggtttgggt ggggctggac ggaaaagttc cgtttgctta ataattcctt ctttttgaac ttcaaccggt tgttgttt

Expected Protein Sequence

MAHHHHHHMT EKQQPVEVQK EGIIKQTELF RPAPPKPVSL EPTIEKNNVL LPKVELPTPP KKTTNSDDSL LEAAQRAPVL AYANTQKGQG STEKNKDISA NQPEAKPDET AQRFNHLLKP TTLEGIRAAK LGNRNYIIAM GASIPCILET AISSDQQGFA SCIVSRDILS DNGRVVLLDK GTQIVGEYRA GLKKGQKRLF VLWNRAKTPN GIIITLASPA TDALGRSGMD GDIDNHWLER IGSALLVSIV KDATNYVKGR LPKDQDKNNS ETISSGQNIA NIAVENYANI PPTLSKNQGE MVNVFVARDL DFSNVYKLKV IENKKQIVNR ALSRNFYKNS AVILQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacag agaaacaaca accggttgaa gttcaaaaag aaggaattat taagcaaacg gaactttttc gtccagcccc acccaaacct gtatccctgg agccaacgat agaaaaaaat aacgttctgt tgcctaaggt tgaactacca actcccccca aaaaaacaac caattctgat gattcactct tagaagcagc acaacgtgct cctgtcttag cctatgccaa cacacaaaaa ggccaaggga gcacagaaaa aaacaaagac ataagcgcga accaacctga agccaagcct gatgaaacag cgcaacgctt taatcatctt ctcaaaccaa caacccttga aggcattcgt gctgcaaaac ttggcaatcg aaattacatc atcgcaatgg gcgcttccat tccgtgtatc ttagaaaccg cgatcagtag tgaccaacaa ggatttgcca gctgtattgt ctcacgagat atcttgtctg ataatggccg cgttgttctt cttgataaag gcacccaaat cgttggcgag tatcgtgctg gattaaaaaa aggacaaaag cgtctctttg ttctatggaa tagagccaaa acaccaaatg gtatcattat caccttagct tcaccggcaa cagatgcctt aggacgttct ggtatggatg gagatattga taaccattgg ttagagagaa ttggctctgc gcttcttgta tcgattgtca aagatgcaac aaactatgtg aaaggccgct tgccaaaaga ccaagataag aacaattctg aaacaatttc ttcaggacaa aacattgcaa atattgccgt agaaaattac gccaatattc cccctaccct gtccaagaac caaggggaaa tggtcaatgt ttttgtggct cgcgatttag atttttccaa tgtttataaa ttgaaagtga tcgaaaacaa aaaacagatt gtcaatcggg ctctttcgag aaacttttat aaaaattctg cggtaatttt gcaatgatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BaheA.18411.a.B2.PS01496

PURIFICATION DATe:	5/21/2012
CONCENTRATION:	22.45mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	58

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAG AGAAACAACA ACCGGTTGAA GTTCAAAAAG AAGGAATTAT TAAGCAAACG GAACTTTTTC GTCCAGCCCC ACCCAAACCT GTATCCCTGG AGCCAACGAT AGAAAAAAAT AACGTTCTGT TGCCTAAGGT TGAACTACCA ACTCCCCCCA AAAAAACAAC CAATTCTGAT GATTCACTCT TAGAAGCAGC ACAACGTGCT CCTGTCTTAG CCTATGCCAA CACACAAAAA GGCCAAGGGA GCACAGAAAA AAACAAAGAC ATAAGCGCGA ACCAACCTGA AGCCAAGCCT GATGAAACAG CGCAACGCTT TAATCATCTT CTCAAACCAA CAACCCTTGA AGGCATTCGT GCTGCAAAAC TTGGCAATCG AAATTACATC ATCGCAATGG GCGCTTCCAT TCCGTGTATC TTAGAAACCG CGATCAGTAG TGACCAACAA GGATTTGCCA GCTGTATTGT CTCACGAGAT ATCTTGTCTG ATAATGGCCG CGTTGTTCTT CTTGATAAAG GCACCCAAAT CGTTGGCGAG TATCGTGCTG GATTAAAAAA AGGACAAAAG CGTCTCTTTG TTCTATGGAA TAGAGCCAAA ACACCAAATG GTATCATTAT CACCTTAGCT TCACCGGCAA CAGATGCCTT AGGACGTTCT GGTATGGATG GAGATATTGA TAACCATTGG TTAGAGAGAA TTGGCTCTGC GCTTCTTGTA TCGATTGTCA AAGATGCAAC AAACTATGTG AAAGGCCGCT TGCCAAAAGA CCAAGATAAG AACAATTCTG AAACAATTTC TTCAGGACAA AACATTGCAA ATATTGCCGT AGAAAATTAC GCCAATATTC CCCCTACCCT GTCCAAGAAC CAAGGGGAAA TGGTCAATGT TTTTGTGGCT CGCGATTTAG ATTTTTCCAA TGTTTATAAA TTGAAAGTGA TCGAAAACAA AAAACAGATT GTCAATCGGG CTCTTTCGAG AAACTTTTAT AAAAATTCTG CGGTAATTTT GCAATGATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT