SSGCID: Target Page

BaheA.18408.c

TrwK protein (Trw type IV secretion machinery)

CENTER ID:	BaheA.18408.c
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.18408.c.B1.GE35056	full length		1	823

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1820
RefSeq:	YP_034262.1
UniProt:	Q6G1T5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMAVESSKRL ASETPVSLFI PYSHHITDTI ISTKNAEYLS IWKIDGRSHQ SASEEDIFQW ARELNNTLRG IASANLSLWT HIVRRRVYEY PDSTFDKMFC YQLDEKYRQS FTGYNLMVND LYLTVVFQPI ADKVMSFFSQ HERETLDQKK MRQESCIKAL NDINRTLGQS FKRYGGELLG TYEKNGHAYS SALEFLAMLV NGEYSPMPIC RDRFADYMSI NRPFFSKWGA LGELRTTSGI RRFGMMEIKE YDTTTKPGQF NVLLESDFEF VLTQSFSVLS RHAAKDYLQR HQRNLIDARD VATSQIEQID EALNQLISGH FVMGEHHCTL TIYGDTVQQV RDYMAKANAA LLEVAVLPKP VDLAIEAGYW AQLPANWKWR PRPAPITSLN FLSFSSFHNF MSGKPTGNPW GPAVTILKTI SKTPLYFNFH ASKLEEDATD KRLLGNTAII GQSGSGKTVL LGFLLAQAQK FKPTTVVFDK DRGMEIAIRA MGGRYLTFKP GRQSGFNPFQ LPPTQDNLTF LKQFVKKLAE ASSEITHRDE EEINQAVMSI MSSNINPSLR RLSFLLQFLP NPHLNDYNSH PSVHTRLIKW CEGGDYGWLF DNPTDALDLS THQIYGFDIT EFLDNPETRT PVMMYLLYRT EGMISGQRFM YIFDEFWKPL QDPYFEDLAK NKQKTIRKQN GIFVFATQEP TDALESNIAK TLIQQCATYI FLANPKANYE DYTEGFKLTD AEFELIKGIG EFSRQFLIKQ GDQSALAELN LGKFHVTIDG KTVEQNFSDE LLVLSGTPDN AELVENIIAE VGDDPALWLP VFLQHIKTNR RTT

NT Sequence

atgatggctg ttgaaagcag caaacgcctc gcgtcagaaa caccagtaag ccttttcata ccctattcac atcatattac agacacgatt atctctacca aaaacgcaga atatttatcg atctggaaga ttgatggtcg ttcgcatcaa agcgcttcag aagaggatat ttttcagtgg gctagagagc taaataatac cttacgtggt attgcgtcgg ctaatttatc tctgtggaca catatcgtgc gccgccgtgt ttatgaatat cccgattcaa catttgataa aatgttttgc taccaactcg atgaaaaata ccggcaaagt tttactggtt ataatttgat ggtcaatgat ctgtatctga ccgttgtttt ccagccgata gctgataaag ttatgtcatt tttttctcag catgaacgtg aaacgcttga tcaaaaaaag atgcgacaag aatcatgtat caaagctctc aatgacatta accgtacctt aggtcagtcg tttaaacgtt acggtggaga acttcttggt acatatgaaa aaaatggtca tgcttattct tctgcccttg aatttctggc aatgcttgtc aatggtgagt actcccctat gccgatctgt cgcgatcgtt ttgctgacta tatgtctatt aatcggcctt tcttctcaaa atggggcgca ctcggtgaac tgcggacaac cagtggaata cgccgatttg gaatgatgga aatcaaagaa tatgacacaa caactaaacc aggacaattt aatgttctgt tggaaagtga ttttgaattt gtgttgacac agagtttttc tgtactctcg cgccacgctg ccaaagatta tttgcagcgg catcagcgta atcttattga tgctcgtgat gtagcaacaa gtcaaattga acaaattgac gaagcattaa atcagctgat aagtgggcat tttgtcatgg gtgaacacca ttgtacactg accatctacg gtgatacagt tcaacaagtt cgtgactata tggcaaaagc gaatgctgca ctgttagaag ttgcagtgtt accaaaacct gtagatttgg ccatagaggc tggttattgg gcgcaacttc ccgcaaattg gaaatggcga ccacgtcccg caccaattac gtcactgaat tttttatcct tctcatcttt tcacaatttt atgtcaggca aaccgaccgg gaatccatgg ggaccggcag tgacaattct taaaacaata agtaaaaccc cgctttactt taattttcat gcttccaaac ttgaagagga tgcgacagat aagcggttac ttggtaatac cgcaatcatt gggcaatctg gttctggtaa aaccgttttg cttgggtttt tgttagcgca agcgcaaaaa ttcaaaccga caactgttgt ttttgataaa gatcgtggca tggaaattgc cattcgagca atgggagggc ggtatttgac atttaagcct ggtaggcaaa gtggtttcaa tccgttccaa cttccaccga cacaagacaa tctcactttt cttaaacaat ttgttaaaaa gttggcagaa gcgagtagtg aaatcacaca ccgcgatgaa gaagagatta accaagctgt catgtccatt atgagtagca acattaaccc atcgttgcgt cgattgtctt ttttgctgca attcttacca aatccgcatt tgaatgatta taattcacat ccatctgttc acacccgctt gataaaatgg tgcgaaggtg gtgattatgg atggttattt gataatccca ctgatgcact tgacctctca acacatcaaa tttacggctt cgatatcact gaatttttgg ataacccaga gactcgtact cccgtgatga tgtatttgct ctatcgcacg gaaggaatga tcagtggaca aaggtttatg tatatctttg acgagttttg gaagccattg caagatccgt attttgaaga tttagcaaaa aataaacaaa aaactattcg taaacaaaat ggtatttttg tttttgcaac acaagagcct actgatgcgc tagagagtaa tattgccaaa acgttgattc agcaatgcgc aacttacatt tttttagcca atccgaaagc aaattatgaa gactatacag aaggtttcaa actaacagat gcagagtttg aactaatcaa aggaattggc gaatttagtc gtcaattcct tatcaagcag ggtgatcaat ctgcccttgc agaacttaat ctcggcaaat tccatgtgac aattgatgga aaaactgtcg aacaaaactt tagcgatgaa cttttggtgt tatcaggtac acctgacaat gcagagttgg ttgaaaatat tatcgctgaa gtgggggatg atccggcact atggttaccg gtttttttac aacatataaa aactaacagg aggacaacat ga

Details for BaheA.18408.c.B1.GE35056

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	95kDa
OBSERVED MW:	95kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	85
PERCENT COVERAGE:	46

Validated AA Sequence

Validated NT Sequence

ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatgatg gctgttgaaa gcagcaaacg cctcgcgtca gaaacaccag taagcctttt cataccctat tcacatcata ttacagacac gattatctct accaaaaacg cagaatattt atcgatctgg aagattgatg gtcgttcgca tcaaagcgct tcagaagagg atatttttca gtgggctaga gagctaaata ataccttacg tggtattgcg tcggctaatt tatctctgtg gacacatatc gtgcgccgcc gtgtttatga atatcccgat tcaacatttg ataaaatgtt ttgctaccaa ctcgatgaaa aataccggca aagttttact ggttataatt tgatggtcaa tgatctgtat ctgaccgttg ttttccagcc gatagctgat aaagttatgt catttttttc tcagcatgaa cgtgaaacgc ttgatcaaaa aaagatgcga caagaatcat gtatcaaagc tctcaatgac attaaccgta ccttaggtca gtcgtttaaa cgttacggtg gagaacttct tggtacatat gaaaaaaatg gtcatgctta ttcttctgcc cttgaatttc tggcaatgct tgtcaatggt gagtactccc ctatgccgat ctgtcgcgat cgttttgctg actatatgtc tattaatcgg cctttcttct caaaatgggg cgcactcggt gaactgcgga caaccagtgg aatacgccga tttggaatga tggaaatcaa agaatatgac acaacaacta aaccaggaca atttaatgtt ctgttggaaa gtgattttga atttgtgttg acacagagtt tttctgtact ctcgcgccac gctgccaaag attatttgca gcggcatcag cgtaatctta ttgatgctcg tgatgtagca acnagtcaaa ttgaacaaat tgacgaanca ttnaatcagc tganantnnn attttgncat ggnnnannnc atngnacnct gannntctan gnnanncnnt ncnnnnnncn ngactnnnaa ncnangctgn annnnnnaan tnncnnnnnn nannnnnann ntnnnnnnnn nnnnnntgnn nnnnnnnncn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntt

Expected Protein Sequence

MAHHHHHHMM AVESSKRLAS ETPVSLFIPY SHHITDTIIS TKNAEYLSIW KIDGRSHQSA SEEDIFQWAR ELNNTLRGIA SANLSLWTHI VRRRVYEYPD STFDKMFCYQ LDEKYRQSFT GYNLMVNDLY LTVVFQPIAD KVMSFFSQHE RETLDQKKMR QESCIKALND INRTLGQSFK RYGGELLGTY EKNGHAYSSA LEFLAMLVNG EYSPMPICRD RFADYMSINR PFFSKWGALG ELRTTSGIRR FGMMEIKEYD TTTKPGQFNV LLESDFEFVL TQSFSVLSRH AAKDYLQRHQ RNLIDARDVA TSQIEQIDEA LNQLISGHFV MGEHHCTLTI YGDTVQQVRD YMAKANAALL EVAVLPKPVD LAIEAGYWAQ LPANWKWRPR PAPITSLNFL SFSSFHNFMS GKPTGNPWGP AVTILKTISK TPLYFNFHAS KLEEDATDKR LLGNTAIIGQ SGSGKTVLLG FLLAQAQKFK PTTVVFDKDR GMEIAIRAMG GRYLTFKPGR QSGFNPFQLP PTQDNLTFLK QFVKKLAEAS SEITHRDEEE INQAVMSIMS SNINPSLRRL SFLLQFLPNP HLNDYNSHPS VHTRLIKWCE GGDYGWLFDN PTDALDLSTH QIYGFDITEF LDNPETRTPV MMYLLYRTEG MISGQRFMYI FDEFWKPLQD PYFEDLAKNK QKTIRKQNGI FVFATQEPTD ALESNIAKTL IQQCATYIFL ANPKANYEDY TEGFKLTDAE FELIKGIGEF SRQFLIKQGD QSALAELNLG KFHVTIDGKT VEQNFSDELL VLSGTPDNAE LVENIIAEVG DDPALWLPVF LQHIKTNRRT T

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgg ctgttgaaag cagcaaacgc ctcgcgtcag aaacaccagt aagccttttc ataccctatt cacatcatat tacagacacg attatctcta ccaaaaacgc agaatattta tcgatctgga agattgatgg tcgttcgcat caaagcgctt cagaagagga tatttttcag tgggctagag agctaaataa taccttacgt ggtattgcgt cggctaattt atctctgtgg acacatatcg tgcgccgccg tgtttatgaa tatcccgatt caacatttga taaaatgttt tgctaccaac tcgatgaaaa ataccggcaa agttttactg gttataattt gatggtcaat gatctgtatc tgaccgttgt tttccagccg atagctgata aagttatgtc atttttttct cagcatgaac gtgaaacgct tgatcaaaaa aagatgcgac aagaatcatg tatcaaagct ctcaatgaca ttaaccgtac cttaggtcag tcgtttaaac gttacggtgg agaacttctt ggtacatatg aaaaaaatgg tcatgcttat tcttctgccc ttgaatttct ggcaatgctt gtcaatggtg agtactcccc tatgccgatc tgtcgcgatc gttttgctga ctatatgtct attaatcggc ctttcttctc aaaatggggc gcactcggtg aactgcggac aaccagtgga atacgccgat ttggaatgat ggaaatcaaa gaatatgaca caacaactaa accaggacaa tttaatgttc tgttggaaag tgattttgaa tttgtgttga cacagagttt ttctgtactc tcgcgccacg ctgccaaaga ttatttgcag cggcatcagc gtaatcttat tgatgctcgt gatgtagcaa caagtcaaat tgaacaaatt gacgaagcat taaatcagct gataagtggg cattttgtca tgggtgaaca ccattgtaca ctgaccatct acggtgatac agttcaacaa gttcgtgact atatggcaaa agcgaatgct gcactgttag aagttgcagt gttaccaaaa cctgtagatt tggccataga ggctggttat tgggcgcaac ttcccgcaaa ttggaaatgg cgaccacgtc ccgcaccaat tacgtcactg aattttttat ccttctcatc ttttcacaat tttatgtcag gcaaaccgac cgggaatcca tggggaccgg cagtgacaat tcttaaaaca ataagtaaaa ccccgcttta ctttaatttt catgcttcca aacttgaaga ggatgcgaca gataagcggt tacttggtaa taccgcaatc attgggcaat ctggttctgg taaaaccgtt ttgcttgggt ttttgttagc gcaagcgcaa aaattcaaac cgacaactgt tgtttttgat aaagatcgtg gcatggaaat tgccattcga gcaatgggag ggcggtattt gacatttaag cctggtaggc aaagtggttt caatccgttc caacttccac cgacacaaga caatctcact tttcttaaac aatttgttaa aaagttggca gaagcgagta gtgaaatcac acaccgcgat gaagaagaga ttaaccaagc tgtcatgtcc attatgagta gcaacattaa cccatcgttg cgtcgattgt cttttttgct gcaattctta ccaaatccgc atttgaatga ttataattca catccatctg ttcacacccg cttgataaaa tggtgcgaag gtggtgatta tggatggtta tttgataatc ccactgatgc acttgacctc tcaacacatc aaatttacgg cttcgatatc actgaatttt tggataaccc agagactcgt actcccgtga tgatgtattt gctctatcgc acggaaggaa tgatcagtgg acaaaggttt atgtatatct ttgacgagtt ttggaagcca ttgcaagatc cgtattttga agatttagca aaaaataaac aaaaaactat tcgtaaacaa aatggtattt ttgtttttgc aacacaagag cctactgatg cgctagagag taatattgcc aaaacgttga ttcagcaatg cgcaacttac atttttttag ccaatccgaa agcaaattat gaagactata cagaaggttt caaactaaca gatgcagagt ttgaactaat caaaggaatt ggcgaattta gtcgtcaatt ccttatcaag cagggtgatc aatctgccct tgcagaactt aatctcggca aattccatgt gacaattgat ggaaaaactg tcgaacaaaa ctttagcgat gaacttttgg tgttatcagg tacacctgac aatgcagagt tggttgaaaa tattatcgct gaagtggggg atgatccggc actatggtta ccggtttttt tacaacatat aaaaactaac aggaggacaa catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat