BaheA.18408.b

TrwK protein (Trw type IV secretion machinery)

CENTER ID: BaheA.18408.b
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.18408.b.B1.GE34967 full length 1 823
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: B0RK98

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAVESSKRL ASETPVSLFI PYSHHITDTI ISTKNAEYLS IWKIDGRSHQ SASEEDIFQW VRELNNTLRG IASANLSLWT HIVRRRVYEY PDSTFDKMFC YQLDEKYRQS FTGYNLMVND LYLTVIFQPI ADKVMSFFSQ HERETLDQKK MRQESSIKAL NDINRTLGQS FKRYGGELLG TYEKNGHAYS SALEFLAMLV NGEYSPMPIC RDRFADYMSI NRPFFSKWGA LGELRTTSGI RRFGMMEIKE YDTTTKPGQF NVLLESDFEF VLTQSFSVLS RHAAKDYLQR HQRNLIDARD VATSQIEQID EALNQLISGH FVMGEHHCTL TIYGDTVQQV RDYMAKANAA LLEVAVLPKP VDLAIEAGYW AQLPANWKWR PRPAPITSLN FLSFSSFHNF MSGKPTGNPW GPAVTILKTI SKTPLYFNFH ASKLEEDATD KRLLGNTAII GQSGSGKTVL LGFLLAQAQK FKPTTVVFDK DRGMEIAIRA MGGRYLTFKP GRQSGFNPFQ LPPTQDNLTF LKQFVKKLAE ASGEITHRDE EEINQAVTSI MSSNIDPSLR RLSFLLQFLP NPRLSDYNSH PSVHTRLIKW CEGGDYGWLF DNPTDALDLS THQIYGFDIT EFLDNPDTRT PVMMYLLYRT EGMISGQRFM YIFDEFWKPL QDPYFEDLAK NKQKTIRKQN GIFVFATQEP TDALESNIAK TLIQQCATYI FLANPKANYE DYTEGFKLTD AEFELIKGIG EFSRQFLIKQ GDQSALAELN LDKFHMTIDG KTVEQNFSDE LLVLSGTPDN AELVENIIAE VGDDPALWLP VFLQHVKTNR RTT
NT Sequence
atgacggctg ttgaaagcag caaacgcctc gcgtcagaaa caccagtaag tctattcata ccctattcac atcatattac agatacgatt atttctacca aaaacgcaga gtatttatcg atctggaaaa ttgatgggcg ttcgcatcaa agcgcttcag aagaggatat ttttcagtgg gttagagagc taaataatac cttgcgtggt attgcatcag ccaatttatc gctgtggaca catatcgtgc gccgccgtgt ttatgaatat cccgattcaa catttgataa aatgttttgc taccaactcg atgaaaaata ccggcaaagt tttactggtt ataatttgat ggtcaatgat ctgtatctga ccgttatttt tcagccgata gctgataaag ttatgtcatt tttttctcag catgaacgtg aaacgcttga tcaaaaaaag atgcgacaag aatcatctat caaagctctc aatgacatca accgtacctt aggtcagtcg tttaaacgtt acggtgggga gcttcttggt acgtatgaaa aaaatggtca tgcttattct tctgcccttg agtttctggc aatgcttgtc aatggtgagt actcgcctat gccgatctgt cgcgatcgtt ttgctgacta tatgtctatt aatcggcctt tcttctcaaa atggggcgca ctcggtgaac tgcggacaac cagtggaata cgccgatttg gaatgatgga aatcaaagaa tatgacacaa caactaaacc aggacaattt aatgttctgt tggaaagtga ttttgaattt gtgttgacac agagtttttc tgtactctcg cgccacgctg ccaaagatta tttgcagcgg catcagcgta atcttattga tgctcgtgat gtagcaacaa gtcaaattga acaaattgac gaagcattaa atcagctgat aagtgggcat tttgtcatgg gtgaacacca ttgtacactg accatctacg gtgatacagt tcaacaagtt cgtgactata tggcaaaagc gaatgctgca ctgttagaag ttgcagtgtt accaaaacct gtagatttgg ccatagaggc tggctattgg gcgcaacttc ccgcaaattg gaaatggcga ccacgtcccg caccaattac gtcactgaat tttttatcct tctcatcttt tcacaatttt atgtcaggca aaccgaccgg gaatccatgg ggaccggcag tgacaattct taaaacaata agtaaaaccc cgctttactt taattttcat gcttccaaac ttgaagagga tgcgacagat aagcggttac ttggtaatac cgcaatcatt gggcaatctg gttctggtaa aaccgttttg cttgggtttt tgttagcgca agcgcaaaaa ttcaaaccga caactgttgt ttttgataaa gatcgtggca tggaaattgc tattcgagca atgggagggc ggtatttgac atttaagcct ggtaggcaaa gtggtttcaa tccgttccaa cttccaccga cacaagacaa tctcactttt cttaaacaat ttgttaaaaa gttggcagaa gcgagtggtg aaattacaca ccgcgacgaa gaagagatta accaagctgt cacgtccatt atgagtagca acattgatcc atcgctgcgt cgattgtctt ttttgctgca attcttacca aatccgcgtc tgagtgatta caattcacat ccatctgttc acacccgttt gataaagtgg tgcgaaggtg gtgattatgg atggttattc gataacccca ctgacgcact tgatctttca acacatcaaa tttacggttt tgatattact gaatttttgg ataatccaga cactcgtact cccgtgatga tgtatttgct ctatcgcacg gaaggaatga tcagtggaca aaggtttatg tatatctttg acgagttttg gaagcctttg caagacccgt attttgaaga cttagcaaaa aataaacaaa aaactatccg taaacaaaat ggtatttttg tttttgcaac acaagagcct actgatgcgc tagagagtaa tattgccaaa acgctgattc agcaatgtgc aacttacatt tttctagcaa atccgaaagc aaattatgaa gactatacag aaggcttcaa actaacagat gcagaatttg aactaatcaa agggattggc gaatttagtc gtcaattcct tatcaagcaa ggagatcaat ctgctcttgc agaacttaat ctcgacaagt ttcatatgac aattgatgga aaaactgtcg aacaaaactt tagcgatgaa cttttagtgt tatcaggtac acctgacaat gcagagttgg ttgaaaatat tatcgctgaa gtgggggatg atccggcact atggttaccg gtttttttac aacatgtaaa aactaatagg aggacaacat ga
Details for BaheA.18408.b.B1.GE34967
HARVESTED ON: 3/19/2012
SEQUENCED ON: 3/22/2012
EXPECTED MW: 95kDa
OBSERVED MW: 95kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 77
PERCENT COVERAGE: 47
Validated AA Sequence
MAHHHHHHMT AVESSKRLAS ETPVSLFIPY SHHITDTIIS TKNAEYLSIW KIDGRSHQSA SEEDIFQWAR ELNNTLRGIA SANLSLWTHI VRRRVYEYPD STFDKMFCYQ LDEKYRQSFT GYNLMVNDLY LTVVFQPIAD KVMSFFSQHE RETLDQKKMR QESCIKALND INRTLGQSFK RYGGELLGTY EKNGHAYSSA LEFLAMLVNG EYSPMPICRD RFADYMSINR PFFSKWGALG ELRTTSGIRR FGMMEIKEYD TTTKPGQFNV LLESDFEFVL TQSFSVLSRX AAKXYLQRHQ RNLIDARDVA XVKXXKXTXI XSAXXXXFXX GXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
ttttnnnnnn tttaagaagg aganatacca tggctcacca ccaccaccac catatgacgg ctgttgaaag cagcaaacgc ctcgcgtcag aaacaccagt aagccttttc ataccctatt cacatcatat tacagacacg attatctcta ccaaaaacgc agaatattta tcgatctgga agattgatgg tcgttcgcat caaagcgctt cagaagagga tatttttcag tgggctagag agctaaataa taccttacgt ggtattgcgt cggctaattt atctctgtgg acacatatcg tgcgccgccg tgtttatgaa tatcccgatt caacatttga taaaatgttt tgctaccaac tcgatgaaaa ataccggcaa agttttactg gttataattt gatggtcaat gatctgtatc tgaccgttgt tttccagccg atagctgata aagttatgtc atttttttct cagcatgaac gtgaaacgct tgatcaaaaa aagatgcgac aagaatcatg tatcaaagct ctcaatgaca ttaaccgtac cttaggtcag tcgtttaaac gttacggtgg agaacttctt ggtacatatg aaaaaaatgg tcatgcttat tcttctgccc ttgaatttct ggcaatgctt gtcaatggtg agtactcccc tatgccgatc tgtcgcgatc gttttgctga ctatatgtct attaatcggc ctttcttctc aaaatggggc gcactcggtg aactgcggac aaccagtgga atacgccgat ttggaatgat ggaaatcaaa gaatatgaca caacaactaa accaggacaa tttaatgttc tgttggaaag tgattttgaa tttgtgttga cacagagttt ttctgtactc tcgcgcnncg ctgccaaana ntatttgcag cggcatcagc gtaatcttat tgatgctcgt gatgtagcan nagtcaaant gancaaantg acnannattn antcagctga nnanngggna tttnnncang ggnnannnnn tnntanantg ncntnnnnnn ngannnnnnn nnnnnnnnnn nnnnnnnnnt nnnannnnnn gnnnnnnnnn nnnnnnntnn nnnntnnnan ncnnnnnnnn nnngnnnnnn nntnnnnnnn nnnncnnnnn nnnngngnnn nnnnannnnn nnnnnnnnnn nnnnncnnnn nnnnnnnnnn nnnntnnttn nt
Expected Protein Sequence
MAHHHHHHMT AVESSKRLAS ETPVSLFIPY SHHITDTIIS TKNAEYLSIW KIDGRSHQSA SEEDIFQWVR ELNNTLRGIA SANLSLWTHI VRRRVYEYPD STFDKMFCYQ LDEKYRQSFT GYNLMVNDLY LTVIFQPIAD KVMSFFSQHE RETLDQKKMR QESSIKALND INRTLGQSFK RYGGELLGTY EKNGHAYSSA LEFLAMLVNG EYSPMPICRD RFADYMSINR PFFSKWGALG ELRTTSGIRR FGMMEIKEYD TTTKPGQFNV LLESDFEFVL TQSFSVLSRH AAKDYLQRHQ RNLIDARDVA TSQIEQIDEA LNQLISGHFV MGEHHCTLTI YGDTVQQVRD YMAKANAALL EVAVLPKPVD LAIEAGYWAQ LPANWKWRPR PAPITSLNFL SFSSFHNFMS GKPTGNPWGP AVTILKTISK TPLYFNFHAS KLEEDATDKR LLGNTAIIGQ SGSGKTVLLG FLLAQAQKFK PTTVVFDKDR GMEIAIRAMG GRYLTFKPGR QSGFNPFQLP PTQDNLTFLK QFVKKLAEAS GEITHRDEEE INQAVTSIMS SNIDPSLRRL SFLLQFLPNP RLSDYNSHPS VHTRLIKWCE GGDYGWLFDN PTDALDLSTH QIYGFDITEF LDNPDTRTPV MMYLLYRTEG MISGQRFMYI FDEFWKPLQD PYFEDLAKNK QKTIRKQNGI FVFATQEPTD ALESNIAKTL IQQCATYIFL ANPKANYEDY TEGFKLTDAE FELIKGIGEF SRQFLIKQGD QSALAELNLD KFHMTIDGKT VEQNFSDELL VLSGTPDNAE LVENIIAEVG DDPALWLPVF LQHVKTNRRT T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg ctgttgaaag cagcaaacgc ctcgcgtcag aaacaccagt aagtctattc ataccctatt cacatcatat tacagatacg attatttcta ccaaaaacgc agagtattta tcgatctgga aaattgatgg gcgttcgcat caaagcgctt cagaagagga tatttttcag tgggttagag agctaaataa taccttgcgt ggtattgcat cagccaattt atcgctgtgg acacatatcg tgcgccgccg tgtttatgaa tatcccgatt caacatttga taaaatgttt tgctaccaac tcgatgaaaa ataccggcaa agttttactg gttataattt gatggtcaat gatctgtatc tgaccgttat ttttcagccg atagctgata aagttatgtc atttttttct cagcatgaac gtgaaacgct tgatcaaaaa aagatgcgac aagaatcatc tatcaaagct ctcaatgaca tcaaccgtac cttaggtcag tcgtttaaac gttacggtgg ggagcttctt ggtacgtatg aaaaaaatgg tcatgcttat tcttctgccc ttgagtttct ggcaatgctt gtcaatggtg agtactcgcc tatgccgatc tgtcgcgatc gttttgctga ctatatgtct attaatcggc ctttcttctc aaaatggggc gcactcggtg aactgcggac aaccagtgga atacgccgat ttggaatgat ggaaatcaaa gaatatgaca caacaactaa accaggacaa tttaatgttc tgttggaaag tgattttgaa tttgtgttga cacagagttt ttctgtactc tcgcgccacg ctgccaaaga ttatttgcag cggcatcagc gtaatcttat tgatgctcgt gatgtagcaa caagtcaaat tgaacaaatt gacgaagcat taaatcagct gataagtggg cattttgtca tgggtgaaca ccattgtaca ctgaccatct acggtgatac agttcaacaa gttcgtgact atatggcaaa agcgaatgct gcactgttag aagttgcagt gttaccaaaa cctgtagatt tggccataga ggctggctat tgggcgcaac ttcccgcaaa ttggaaatgg cgaccacgtc ccgcaccaat tacgtcactg aattttttat ccttctcatc ttttcacaat tttatgtcag gcaaaccgac cgggaatcca tggggaccgg cagtgacaat tcttaaaaca ataagtaaaa ccccgcttta ctttaatttt catgcttcca aacttgaaga ggatgcgaca gataagcggt tacttggtaa taccgcaatc attgggcaat ctggttctgg taaaaccgtt ttgcttgggt ttttgttagc gcaagcgcaa aaattcaaac cgacaactgt tgtttttgat aaagatcgtg gcatggaaat tgctattcga gcaatgggag ggcggtattt gacatttaag cctggtaggc aaagtggttt caatccgttc caacttccac cgacacaaga caatctcact tttcttaaac aatttgttaa aaagttggca gaagcgagtg gtgaaattac acaccgcgac gaagaagaga ttaaccaagc tgtcacgtcc attatgagta gcaacattga tccatcgctg cgtcgattgt cttttttgct gcaattctta ccaaatccgc gtctgagtga ttacaattca catccatctg ttcacacccg tttgataaag tggtgcgaag gtggtgatta tggatggtta ttcgataacc ccactgacgc acttgatctt tcaacacatc aaatttacgg ttttgatatt actgaatttt tggataatcc agacactcgt actcccgtga tgatgtattt gctctatcgc acggaaggaa tgatcagtgg acaaaggttt atgtatatct ttgacgagtt ttggaagcct ttgcaagacc cgtattttga agacttagca aaaaataaac aaaaaactat ccgtaaacaa aatggtattt ttgtttttgc aacacaagag cctactgatg cgctagagag taatattgcc aaaacgctga ttcagcaatg tgcaacttac atttttctag caaatccgaa agcaaattat gaagactata cagaaggctt caaactaaca gatgcagaat ttgaactaat caaagggatt ggcgaattta gtcgtcaatt ccttatcaag caaggagatc aatctgctct tgcagaactt aatctcgaca agtttcatat gacaattgat ggaaaaactg tcgaacaaaa ctttagcgat gaacttttag tgttatcagg tacacctgac aatgcagagt tggttgaaaa tattatcgct gaagtggggg atgatccggc actatggtta ccggtttttt tacaacatgt aaaaactaat aggaggacaa catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat