SSGCID: Target Page

BaheA.18407.c

TrwE protein (Trw type IV secretion machinery)

CENTER ID:	BaheA.18407.c
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.18407.c.B2.GE34960	soluble domain		75	395

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1829
RefSeq:	YP_034271.1
UniProt:	A0A0H3M4R8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFGNKKGDEI NKGAEQNYAE QKHEKSYTEY IEGAYGSSEL ASERRPIIPG ARTLLIVGLI VIVAVPIALT WKALKMRNTV NVEEEKPQQT VQQIIPSYTP RVIEEPKVIE EPEKPTQTEE LTQSIPPALA QLLQTTMPPH LMQDSEELAR KRMLSSGLNN GGSSESSSEH PKGNTSGNER NNGVLFDQLQ PVRLGQSHAA QLHNRDLLIT QGTQIDCTLE TKIVTSQPGM TTCHLTRDVY STSGRVVLLD RGSKVVGFYQ SGIQQGQTRV FVQWSRIETP SGVIINLDSP GTGPLGEAGI GGWIDTHFWQ RFSGAIMVSI IGNLGEWVKG KINYGNKEKS SSQNAPQQSP ELMVNEILQS SINIPPTLYK NQGERVNIFI ARDLDFSDVY SLVTR

NT Sequence

atgtttggca ataagaaagg agatgaaatc aacaaaggtg cagaacaaaa ctacgccgag caaaaacatg aaaaaagcta taccgaatat attgaaggtg cgtatggtag ctccgaatta gcttcggaac gccgtccaat aatccccggt gcacgtacat tgttaatagt aggactcatc gtcatagtag cagtaccgat tgctttgact tggaaggctt taaaaatgcg caatactgta aatgttgaag aagaaaagcc tcaacaaacg gtacaacaaa ttataccgag ctatacccct cgtgtcatag aagaaccgaa agtaatagaa gaaccagaaa aaccaacaca aacagaagag ctaacgcaat ccattccacc agccttagca cagctgcttc aaacaacgat gccaccacat ttgatgcagg attctgagga attggcacgc aagcgtatgt taagttccgg tcttaacaac ggtggtagta gtgaaagctc ttcagagcat ccaaaaggca acacatccgg taacgaaaga aataatggcg tattattcga tcagctccag cctgtacgat taggtcaatc ccatgcggct caactccaca accgtgatct attaattacg cagggaacac agatagattg cacgctggaa acaaagatcg ttacatcgca gccaggaatg acaacatgtc atttaacacg ggatgtttat tctacgagtg gtcgcgttgt tttgctagac cgtggttcca aagttgttgg tttctatcaa agcggcatac aacaaggaca aacacgtgtt ttcgtgcaat ggtcgcgtat tgaaacccct tctggtgtta tcatcaatct tgactctcct ggtacaggcc cccttggtga agctggtatc ggtggttgga ttgatacaca tttttggcaa cgatttagtg gtgcaattat ggtgagcatc atcggtaatt tgggagaatg ggtaaaaggc aaaattaatt acggaaataa ggaaaaatca tcatctcaaa atgcaccaca acagagtcct gaattaatgg ttaatgaaat tcttcaaagc tccatcaaca ttccgccaac actttacaaa aaccaaggcg agcgagtaaa tatttttatt gctcgtgatc tggatttcag cgatgtctac agtctcgtta cacgttaa

Details for BaheA.18407.c.B2.GE34960

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/22/2012
EXPECTED MW:	36kDa
OBSERVED MW:	0kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK MRNTVNVEEE KPQQTVQQII PSYTPRVIEE PKVIEEPEKP TQTEELTQSI PPALAQLLQT TMPPHLMQDS EELARKRMLS SGLNNGGSSE SSSEHPKGNT SGNERNNGVL FDQLQPVRLG QSHAAQLHNR DLLITQGTQI DCTLETKIVT SQPGMTTCHL TRDVYSTSGR VVLLDRGSKV VGFYQSGIQQ GQTRVFVQWS RIETPSGVII NLDSPGTGPL GEAGIGGWID THFWQRFSGA IMVSIIGNLG EWVKGKINYG NKEKSSSQNA PQQSPELMVN EILQSSINIP PTLYKNQGER VNIFIARDLD FSDVYSLVTR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tgcgcaatac tgtaaatgtt gaagaagaaa agcctcaaca aacggtacaa caaattatac cgagctatac ccctcgtgtc atagaagaac cgaaagtaat agaagaacca gaaaaaccaa cacaaacaga agagctaacg caatccattc caccagcctt agcacagctg cttcaaacaa cgatgccacc acatttgatg caggattctg aggaattggc acgcaagcgt atgttaagtt ccggtcttaa caacggtggt agtagtgaaa gctcttcaga gcatccaaaa ggcaacacat ccggtaacga aagaaataat ggcgtattat tcgatcagct ccagcctgta cgattaggtc aatcccatgc ggctcaactc cacaaccgtg atctattaat tacgcaggga acacagatag attgcacgct ggaaacaaag atcgttacat cgcagccagg aatgacaaca tgtcatttaa cacgggatgt ttattctacg agtggtcgcg ttgttttgct agaccgtggt tccaaagttg ttggtttcta tcaaagcggc atacaacaag gacaaacacg tgttttcgtg caatggtcgc gtattgaaac cccttctggt gttatcatca atcttgactc tcctggtaca ggcccccttg gtgaagctgg tatcggtggt tggattgata cacatttttg gcaacgattt agtggtgcaa ttatggtgag catcatcggt aatttgggag aatgggtaaa aggcaaaatt aattacggaa ataaggaaaa atcatcatct caaaatgcac cacaacagag tcctgaatta atggttaatg aaattcttca aagctccatc aacattccgc caacacttta caaaaaccaa ggcgagcgag taaatatttt tattgctcgt gatctggatt tcagcgatgt ctacagtctc gttacacgtt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat