BaheA.18407.a

TrwD-like protein (Trw type IV secretion machinery)

CENTER ID: BaheA.18407.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.18407.a.B2.GE34962 soluble domain 76 396
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1829
UniProt: Q4L2V1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMFGNKKGDE INKGAEQNYA EQKHEKSYTE YIEGAYGSSE LASERRPIIP GARTLLIVGL IVIVAVPIAL TWKALKMRNT VNVEEEKPQQ TVQQIIPSYT PRVIEEPKVI EEPEKPTQTE ELTQSIPPAL AQLLQTTMPP HLMQDSEELA RKRMLSSGLN NGGSSESSSE HPKGNTSGNE RNNGVLFDQL QPVRLGQSHA AQLHNRDLLI TQGTQIDCTL ETKIVTSQPG MTTCHLTRDV YSTSGRVVLL DRGSKVVGFY QSGIQQGQTR VFVQWSRIET PSGVIINLDS PGTGPLGEAG IGGWIDTHFW QRFSGAIMVS IIGNLGEWVK GKINYGNKEK SSSQNAPQQS PELMVNEILQ SSINIPPTLY KNQGERVNIF IARDLDFSDV YSLVTR
NT Sequence
atgatgtttg gcaataagaa aggagatgaa atcaacaaag gtgcagaaca aaactacgcc gagcaaaaac atgaaaaaag ctataccgaa tatattgaag gtgcgtatgg tagctccgaa ttagcttcgg aacgccgtcc aataatcccc ggtgcacgta cattgttaat agtaggactc atcgtcatag tagcagtacc gattgctttg acttggaagg ctttaaaaat gcgcaatact gtaaatgttg aagaagaaaa gcctcaacaa acggtacaac aaattatacc gagctatacc cctcgtgtca tagaagaacc gaaagtaata gaagaaccag aaaaaccaac acaaacagaa gagctaacgc aatccattcc accagcctta gcacagctgc ttcaaacaac gatgccacca catttgatgc aggattctga ggaattggca cgcaagcgta tgttaagttc cggtcttaac aacggtggta gtagtgaaag ctcttcagag catccaaaag gcaacacatc cggtaatgaa agaaataatg gcgtattatt cgatcagctc cagcctgtac gattaggtca atcccatgcg gctcaactcc ataaccgtga tctattaatt acgcagggaa cacagataga ttgcacgctg gaaacaaaga tcgttacatc gcagccagga atgacaacat gtcatttaac acgggatgtt tattctacga gtggtcgcgt tgttttgcta gaccgtggtt ccaaagtcgt tggtttctat caaagcggca tacaacaagg acaaacacgt gttttcgtgc aatggtcgcg tattgaaacc ccttctggtg ttatcatcaa tcttgactct cccggtacag gcccccttgg tgaagctggt atcggtggtt ggattgatac acatttttgg caacgattta gtggtgcaat catggtgagc atcatcggta atttgggaga atgggtaaaa ggcaaaatta attacggaaa taaggaaaaa tcatcatctc aaaatgcacc acaacagagt cctgaattaa tggttaatga aattcttcaa agctccatca acattccgcc aacactttac aaaaaccaag gcgagcgagt aaatattttt attgctcgtg atctggattt cagcgatgtc tacagtctcg ttacacgtta a
Details for BaheA.18407.a.B2.GE34962
HARVESTED ON: data unavailable
SEQUENCED ON: 3/22/2012
EXPECTED MW: 36kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 86
Validated AA Sequence
MAHHHHHHMK MRNTVNVEEE KPQQTVQQII PSYTPRVIEE PKVIEEPEKP TQTEELTQSI PPALAQLLQT TMPPHLMQDS EELARKRMLS SGLNNGGSSE SSSEHPKGNT SGNERNNGVL FDQLQPVRLG QSHAAQLHNR DLLITQGTQI DCTLETKIVT SQPGMTTCHL TRDVYSTSGR VVLLDRGSKV VGFYXSGIQQ GQTRVFVQXS RIETPSGVII NLDSPGTGPL GEXGIXGWID THFWQRFSGA IMVSIIGNLG EXXKXKINYG NXEKSSSQXH XNRS
Validated NT Sequence
tttgtttact ttaagaagga gatataccat ggctcaccac caccaccacc atatgaaaat gcgcaatact gtaaatgttg aagaagaaaa gcctcaacaa acggtacaac aaattatacc gagctatacc cctcgtgtca tagaagaacc gaaagtaata gaagaaccag aaaaaccaac acaaacagaa gagctaacgc aatccattcc accagcctta gcacagctgc ttcaaacaac gatgccacca catttgatgc aggattctga ggaattggca cgcaagcgta tgttaagttc cggtcttaac aacggtggta gtagtgaaag ctcttcagag catccaaaag gcaacacatc cggtaatgaa agaaataatg gcgtattatt cgatcagctc cagcctgtac gattaggtca atcccatgcg gctcaactcc ataaccgtga tctattaatt acgcagggaa cacagataga ttgcacgctg gaaacaaaga tcgttacatc gcagccagga atgacaacat gtcatttaac acgggatgtt tattctacga gtggtcgcgt tgttttgcta gaccgtggtt ccaaagtcgt tggtttctat canagcggca tacaacaagg acaaacacgt gttttcgtgc aatgntcgcg tattgaaacc ccttctggtg ttatcatcaa tcttgactct cccggnacag gcccccttgg tgaanctggn atcngtggnt ggattgatac acatttttgg caacgattta gtggtgcaat catggtgagc atcatcggta atttgggaga atgnnnnaaa ngcaaaatta attacggaaa taangaaaaa tcatcatctc aantgcaccn caacagatcc tgaattaatg gntaatgaaa ttcttcanan ctccntcanc attccgccaa cactttacaa aaccanngnn agcnagtaan tantttttan tgntcgtgan ctggntttcn ncnangtctn nngnctcnnn cncgtnnant gannanannn nncgnnnnnn nnncnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaaaaan nnnnnnnaaa naaaannna
Expected Protein Sequence
MAHHHHHHMK MRNTVNVEEE KPQQTVQQII PSYTPRVIEE PKVIEEPEKP TQTEELTQSI PPALAQLLQT TMPPHLMQDS EELARKRMLS SGLNNGGSSE SSSEHPKGNT SGNERNNGVL FDQLQPVRLG QSHAAQLHNR DLLITQGTQI DCTLETKIVT SQPGMTTCHL TRDVYSTSGR VVLLDRGSKV VGFYQSGIQQ GQTRVFVQWS RIETPSGVII NLDSPGTGPL GEAGIGGWID THFWQRFSGA IMVSIIGNLG EWVKGKINYG NKEKSSSQNA PQQSPELMVN EILQSSINIP PTLYKNQGER VNIFIARDLD FSDVYSLVTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tgcgcaatac tgtaaatgtt gaagaagaaa agcctcaaca aacggtacaa caaattatac cgagctatac ccctcgtgtc atagaagaac cgaaagtaat agaagaacca gaaaaaccaa cacaaacaga agagctaacg caatccattc caccagcctt agcacagctg cttcaaacaa cgatgccacc acatttgatg caggattctg aggaattggc acgcaagcgt atgttaagtt ccggtcttaa caacggtggt agtagtgaaa gctcttcaga gcatccaaaa ggcaacacat ccggtaatga aagaaataat ggcgtattat tcgatcagct ccagcctgta cgattaggtc aatcccatgc ggctcaactc cataaccgtg atctattaat tacgcaggga acacagatag attgcacgct ggaaacaaag atcgttacat cgcagccagg aatgacaaca tgtcatttaa cacgggatgt ttattctacg agtggtcgcg ttgttttgct agaccgtggt tccaaagtcg ttggtttcta tcaaagcggc atacaacaag gacaaacacg tgttttcgtg caatggtcgc gtattgaaac cccttctggt gttatcatca atcttgactc tcccggtaca ggcccccttg gtgaagctgg tatcggtggt tggattgata cacatttttg gcaacgattt agtggtgcaa tcatggtgag catcatcggt aatttgggag aatgggtaaa aggcaaaatt aattacggaa ataaggaaaa atcatcatct caaaatgcac cacaacagag tcctgaatta atggttaatg aaattcttca aagctccatc aacattccgc caacacttta caaaaaccaa ggcgagcgag taaatatttt tattgctcgt gatctggatt tcagcgatgt ctacagtctc gttacacgtt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat