BaheA.18405.a

TrwJ1 protein (Trw type IV secretion machinery)

CENTER ID: BaheA.18405.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.18405.a.B2.GE35050 mature protein 23 273
BaheA.18405.a.HZ2.GE41708 mature protein 23 273
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: B0RK73

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKMVFTITI SAILATQNLA LAFPFGSSWG FGNSSGINRL GNTWNYRDFT TFGGLDISKL QHATKMEEEL RKAFLEYEKK QEIQKAIKEL PQFKNLEITK DILEKIYKSI TGKRNLGIVV EDSTSFFLKN PQYIYDPDKS SGMSSSVRSV LQKEQISNSI SEARKAIERR QQYATVVDKA VSLQTFQEAE NRFKQIIKVL ASVEKTKDLK DIAELQAHLK KKLAMIQNES AKLQMVAHLR NAEQGLINQQ KHKRNMKILN SKNTAMPPIR SIR
NT Sequence
atgaaaaaaa tggtttttac aataacgata agtgcaattc ttgcaacgca aaacctagct cttgcatttc cttttggaag ttcttggggt tttggaaatt cttcaggtat aaaccgtcta ggaaatactt ggaattatag agatttcact acctttggag gattggatat ttctaaatta caacatgcaa caaaaatgga agaggaatta cgaaaggcat ttttagagta cgaaaagaag caagagatac aaaaagcaat aaaagaattg ccgcaattca aaaaccttga aataaccaaa gatatacttg aaaaaatata taagtctata acaggaaagc gaaatttggg cattgtagta gaagattcca ccagtttttt tctcaaaaat ccacagtata tctacgatcc ggataaaagt tctggtatgt cttcatcagt cagaagcgtt ctgcaaaaag aacaaatttc taattctatc agtgaagcac gtaaagctat tgaaagacgt caacaatacg caacggttgt cgataaagct gtaagtttgc aaacttttca agaagcagaa aatcgtttta aacaaattat aaaagtatta gccagcgtcg aaaaaacaaa agatctgaaa gatattgccg aattacaagc gcatcttaaa aagaaattag ctatgatcca aaacgaaagt gcaaaattgc aaatggtcgc gcatctacgc aacgcagaac aaggacttat caaccaacaa aaacacaaac gcaatatgaa aattcttaat agtaaaaata cagcaatgcc acctatacgg tctatacggt ag
Details for BaheA.18405.a.B2.GE35050
HARVESTED ON: 4/10/2012
SEQUENCED ON: 10/23/2013
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 83
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHMF PFGSSWISGN SFGITRLGDT WDYRDFTTLG GFNLPKLRHA EKMEEELQKL FIEHEKKQKQ KDLKITNDIL EKIYKSITGK RDLGIAALDS TSFFLKNPEY IYDPDKNSGM SSSVKSILQK EQISDIREAR KSIEGREQYA TVVDKAVSFQ TFQEAENRFK QIAKMLADVE KTKDLKDIAE LQAHIKSKLA MIQNESAKLQ MVAHLRNAEQ GLINQQKHKR NMKILDSKNT AMPPIRSIR
Validated NT Sequence
atggctcacc accaccacca ccatatgttt ccttttggaa gttcttggat tagtggaaat tcttttggta taacccgttt gggagatact tgggattata gagatttcac tacccttgga gggtttaatc ttcctaaatt gcgacatgca gaaaaaatgg aagaggaatt acaaaagcta tttatagaac acgaaaagaa gcagaagcaa aaggacttaa agataaccaa cgatatactt gaaaaaatat ataagtctat aacaggaaaa cgagatttgg gcattgcagc actagattct accagttttt ttctcaaaaa cccagagtat atctacgatc cggataaaaa ttctggtatg tcttcatcag tcaaaagcat tctacaaaag gaacaaattt ctgatatccg tgaagctcga aaatctattg aaggacgcga acaatacgca acggttgtcg ataaagccgt aagttttcaa acttttcaag aagcagaaaa tcgttttaaa caaattgcaa aaatgttagc cgacgtcgaa aaaacaaaag atctgaaaga tattgccgaa ttacaagcgc atattaaaag taaattagct atgatccaaa acgaaagtgc aaaattgcaa atggttgcgc atctacgcaa cgcagaacaa ggacttatca accaacaaaa acacaaacgt aatatgaaaa ttcttgatag taaaaataca gcaatgccac ctatacggtc tatacggtaa gtgagtaaga taggatccgg
Expected Protein Sequence
MAHHHHHHMF PFGSSWGFGN SSGINRLGNT WNYRDFTTFG GLDISKLQHA TKMEEELRKA FLEYEKKQEI QKAIKELPQF KNLEITKDIL EKIYKSITGK RNLGIVVEDS TSFFLKNPQY IYDPDKSSGM SSSVRSVLQK EQISNSISEA RKAIERRQQY ATVVDKAVSL QTFQEAENRF KQIIKVLASV EKTKDLKDIA ELQAHLKKKL AMIQNESAKL QMVAHLRNAE QGLINQQKHK RNMKILNSKN TAMPPIRSIR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttc cttttggaag ttcttggggt tttggaaatt cttcaggtat aaaccgtcta ggaaatactt ggaattatag agatttcact acctttggag gattggatat ttctaaatta caacatgcaa caaaaatgga agaggaatta cgaaaggcat ttttagagta cgaaaagaag caagagatac aaaaagcaat aaaagaattg ccgcaattca aaaaccttga aataaccaaa gatatacttg aaaaaatata taagtctata acaggaaagc gaaatttggg cattgtagta gaagattcca ccagtttttt tctcaaaaat ccacagtata tctacgatcc ggataaaagt tctggtatgt cttcatcagt cagaagcgtt ctgcaaaaag aacaaatttc taattctatc agtgaagcac gtaaagctat tgaaagacgt caacaatacg caacggttgt cgataaagct gtaagtttgc aaacttttca agaagcagaa aatcgtttta aacaaattat aaaagtatta gccagcgtcg aaaaaacaaa agatctgaaa gatattgccg aattacaagc gcatcttaaa aagaaattag ctatgatcca aaacgaaagt gcaaaattgc aaatggtcgc gcatctacgc aacgcagaac aaggacttat caaccaacaa aaacacaaac gcaatatgaa aattcttaat agtaaaaata cagcaatgcc acctatacgg tctatacggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaheA.18405.a.HZ2.GE41708
HARVESTED ON: 6/19/2017
SEQUENCED ON: 6/22/2017
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 88
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMF PFGSSWISGN SFGITRLGDT WDYRDFTTLG GFNLPKLRHA EKMEEELQKL FIEHEKKQKQ KDLKITNDIL EKIYKSITGK RDLGIAALDS TSFFLKNPEY IYDPDKNSGM SSSVKSILQK EQISDIREAR KSIEGREQYA TVVDKAVSFQ TFQEAENRFK QIAKMLADVE KTKDLKDIAE LQAHIKSKLA MIQNESAKLQ MVAHLRNAEQ GLINQQKHKR NMKILDSKNT AMPPIRSIR
Validated NT Sequence
ttaccgtata gaccgtatag gtggcattgc tgtattttta ctatcaagaa ttttcatatt acgtttgtgt ttttgttggt tgataagtcc ttgttctgcg ttgcgtagat gcgcaaccat ttgcaatttt gcactttcgt tttggatcat agctaattta cttttaatat gcgcttgtaa ttcggcaata tctttcagat cttttgtttt ttcgacgtcg gctaacattt ttgcaatttg tttaaaacga ttttctgctt cttgaaaagt ttgaaaactt acggctttat cgacaaccgt tgcgtattgt tcgcgtcctt caatagattt tcgagcttca cggatatcag aaatttgttc cttttgtaga atgcttttga ctgatgaaga cataccagaa tttttatccg gatcgtagat atactctggg tttttgagaa aaaaactggt agaatctagt gctgcaatgc ccaaatctcg ttttcctgtt atagacttat atattttttc aagtatatcg ttggttatct ttaagtcctt ttgcttctgc ttcttttcgt gttctataaa tagcttttgt aattcctctt ccattttttc tgcatgtcgc aatttaggaa gattaaaccc tccaagggta gtgaaatctc tataatccca agtatctccc aaacgggtta taccaaaaga atttccacta atccaagaac ttccaaaagg aaacatcgaa ccaggaccct gggtctgagc ttccagggta ccaccaatct gttctctgtg agcctcaata atatcgttat cctccatgtc caaatcttca ggggtctgat cagcttgaat tctaataccg tcgtacaaga atcttaagga gtccatttcc ttaccctgtc ttttagcgaa cgcttccatc agccttctta aaggagtggt ctttttgatc ttgaagaaga tctctgaaga tccatcggac acctttaaat tgatgtgagt ctcaggcttg acttctggct tgacctctgg cttagcttct tgattgactt ctgagtccga cttcatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSFP FGSSWGFGNS SGINRLGNTW NYRDFTTFGG LDISKLQHAT KMEEELRKAF LEYEKKQEIQ KAIKELPQFK NLEITKDILE KIYKSITGKR NLGIVVEDST SFFLKNPQYI YDPDKSSGMS SSVRSVLQKE QISNSISEAR KAIERRQQYA TVVDKAVSLQ TFQEAENRFK QIIKVLASVE KTKDLKDIAE LQAHLKKKLA MIQNESAKLQ MVAHLRNAEQ GLINQQKHKR NMKILNSKNT AMPPIRSIR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgt ttccttttgg aagttcttgg ggttttggaa attcttcagg tataaaccgt ctaggaaata cttggaatta tagagatttc actacctttg gaggattgga tatttctaaa ttacaacatg caacaaaaat ggaagaggaa ttacgaaagg catttttaga gtacgaaaag aagcaagaga tacaaaaagc aataaaagaa ttgccgcaat tcaaaaacct tgaaataacc aaagatatac ttgaaaaaat atataagtct ataacaggaa agcgaaattt gggcattgta gtagaagatt ccaccagttt ttttctcaaa aatccacagt atatctacga tccggataaa agttctggta tgtcttcatc agtcagaagc gttctgcaaa aagaacaaat ttctaattct atcagtgaag cacgtaaagc tattgaaaga cgtcaacaat acgcaacggt tgtcgataaa gctgtaagtt tgcaaacttt tcaagaagca gaaaatcgtt ttaaacaaat tataaaagta ttagccagcg tcgaaaaaac aaaagatctg aaagatattg ccgaattaca agcgcatctt aaaaagaaat tagctatgat ccaaaacgaa agtgcaaaat tgcaaatggt cgcgcatcta cgcaacgcag aacaaggact tatcaaccaa caaaaacaca aacgcaatat gaaaattctt aatagtaaaa atacagcaat gccacctata cggtctatac ggtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa