BaheA.17838.a

Histidine kinase, putative

CENTER ID: BaheA.17838.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.17838.a.A1.GE34615 full length 1 485
BaheA.17838.a.A2.GE34610 sensor histidine kinase domain 259 485
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.17838.a.A2.PW35220 sensor histidine kinase domain 259 485

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1524
RefSeq: YP_034089.1
UniProt: A0A0H3M6M7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIFRFADGN SMTTKTNLLP HIPSSDAIIS RWRWLAIIVS LVMALLASAF ILLLSNAVDR EVAQLNTSSE LREQADLVLI SLIDMAVADR SYAKNHKAED KARFEHAARN LDDILEYIEL IVYAHPQWYE WFTSFKKEVE ARKAFMNDHM RALDALPDQS SQPSSSSEIP KIQVARLAQL MTSFINGDDV VRQKQREGIA LMRAALTLAA IVSVVSTFMS AYFVINRFHR DVRSLKMYQL LLHSENIALE ARVKERTQEL ERARNHAEKE RQRVEMLLQD VSHRIGNSLG TVSSLLGLQL NRSDNEEVRS VLGAARDRIQ TISTAHRRLR LSDDMETTLL AEFLSSVVHD VELAMPLELR ENITIHTNFK ECRLSSRDAT TLGIILGELL TNSLKHAFPD KRSGDIYISF GPQKNGQLML IVEDNGVGMK NQTQEKKTGL GRVVVEQLCM QFGEKPLFET SALGGTKIVI PLPKLTPNAS KEAYA
NT Sequence
atgacgattt tcaggttcgc cgatgggaat tctatgacaa caaagacgaa tttgctccct catataccct cttctgatgc aataatatcg cgttggcgtt ggttagcgat tattgtttct cttgttatgg ctctgttggc ttcggccttt attttgttac tctcaaacgc cgttgatcgc gaagttgcac agttgaatac aagttcagaa ttgcgtgaac aagctgattt ggtgcttatt agtttgatcg atatggccgt agctgaccgc agttatgcaa aaaaccacaa ggcagaagat aaagcacgct ttgaacatgc agctcgtaat cttgatgata ttctcgagta tattgaactt attgtttacg cacatccgca gtggtatgaa tggtttactt cttttaaaaa agaggtcgaa gcacgcaaag cctttatgaa tgaccatatg cgcgctcttg atgcgcttcc tgatcaatct tctcaaccct cttcttcttc tgaaatccca aaaattcaag tggcgcgttt agcgcaattg atgacaagct ttattaatgg cgatgatgtg gtgcggcaaa aacagcgcga gggaatcgca ctgatgcggg ctgctttaac attggctgct attgtttctg tcgtgagtac tttcatgtcg gcctattttg tcattaatcg ttttcaccgt gatgtacgct ctttaaaaat gtatcaattg ctgcttcata gtgaaaatat agcccttgaa gctcgggtaa aagaacgcac acaagaattg gaaagagcac gcaatcacgc cgagaaagaa cgacaacgtg ttgaaatgtt gttgcaagat gtgagccatc gtattggcaa ctctcttggt acggtctctt ctttgctagg gctccaatta aatcgcagtg acaatgaaga ggtgcgttct gttcttggtg cagcacgtga ccgtatccag acaatctcca cagcgcatcg tcgtttgcgt ttgagtgatg atatggaaac aactttattg gcagagtttc tcagttcggt agtgcatgat gttgaattgg caatgcctct tgaattgcgg gaaaatatca ccattcacac gaactttaaa gagtgccgat tgtcttcaag agatgcgacg acattgggca ttattcttgg tgagttgttg acaaactcct taaaacatgc ctttcctgat aaacgctctg gtgatattta catctcattt ggtcctcaga aaaatgggca actgatgctg attgtggaag ataatggtgt gggcatgaag aatcaaacgc aagagaaaaa aacaggtctt ggtcgggtgg tcgttgaaca gctttgtatg cagtttggtg aaaaaccgct ttttgaaact tctgctttgg gaggaacaaa aattgtaatt cctttgccca aattaacccc caatgcctca aaagaagcct atgcttga
Details for BaheA.17838.a.A1.GE34615
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTIFRFADG NSMTTKTNLL PHIPSSDAII SRWRWLAIIV SLVMALLASA FILLLSNAVD REVAQLNTSS ELREQADLVL ISLIDMAVAD RSYAKNHKAE DKARFEHAAR NLDDILEYIE LIVYAHPQWY EWFTSFKKEV EARKAFMNDH MRALDALPDQ SSQPSSSSEI PKIQVARLAQ LMTSFINGDD VVRQKQREGI ALMRAALTLA AIVSVVSTFM SAYFVINRFH RDVRSLKMYQ LLLHSENIAL EARVKERTQE LERARNHAEX ERQRVEMLLQ DVSHRIGNSL GTVSSXLGLQ LNRSDNEEVR SVLGAARDRI XXXSTAHRRL RLSDDMETTL LAEFLSSVVH DVELAMPLEL RENITIHTNF KECRLSSRDA TTLGIILGEL LTNSLKHAFP DKRSGDIYIS FGPQKNGQLM LIVEDNGVGM KNQTQEKKTG LGRVVVEQLC MQFGEKPLFE TSALGGTKIV IPLPKLTPNA SKEA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacga ttttcaggtt cgccgatggg aattctatga caacaaagac gaatttgctc cctcatatac cctcttctga tgcaataata tcgcgttggc gttggttagc gattattgtt tctcttgtta tggctctgtt ggcttcggcc tttattttgt tactctcaaa cgccgttgat cgcgaagttg cacagttgaa tacaagttca gaattgcgtg aacaagctga tttggtgctt attagtttga tcgatatggc cgtagctgac cgcagttatg caaaaaacca caaggcagaa gataaagcac gctttgaaca tgcagctcgt aatcttgatg atattctcga gtatattgaa cttattgttt acgcacatcc gcagtggtat gaatggttta cttcttttaa aaaagaggtc gaagcacgca aagcctttat gaatgaccat atgcgcgctc ttgatgcgct tcctgatcaa tcttctcaac cctcttcttc ttctgaaatc ccaaaaattc aagtggcgcg tttagcgcaa ttgatgacaa gctttattaa tggcgatgat gtggtgcggc aaaaacagcg cgagggaatc gcactgatgc gggctgcttt aacattggct gctattgttt ctgtcgtgag tactttcatg tcggcctatt ttgtcattaa tcgttttcac cgtgatgtac gctctttaaa aatgtatcaa ttgctgcttc atagtgaaaa tatagccctt gaagctcggg taaaagaacg cacacaagaa ttggaaagag cacgcaatca cgccgagaan gaacgacaac gtgttgaaat gttgttgcaa gatgtgagcc atcgtattgg caactctctt ggtacggtct cttctntgct agggctccaa ttaaatcgca gtgacaatga agaggtgcgt tctgttcttg gtgcagcacg tgaccgtatc cannnnnnct ccacagcgca tcgtcgtttg cgtttgagtg atgatatgga aacaacttta ttggcagagt ttctcagttc ggtagtgcat gatgttgaat tggcaatgcc tcttgaattg cgggaaaata tcaccattca cacgaacttt aaagagtgcc gattgtcttc aagagatgcg acgacattgg gcattattct tggtgagttg ttgacaaact ccttaaaaca tgcctttcct gataaacgct ctggtgatat ttacatctca tttggtcctc agaaaaatgg gcaactgatg ctgattgtgg aagataatgg tgtgggcatg aagaatcaaa cgcaagagaa aaaaacaggt cttggtcggg tggtcgttga acagctttgt atgcagtttg gtgaaaaacc gctttttgaa acttctgctt tgggaggaac aaaaattgta attcctttgc ccaaattaac ccccaatgcc tcaaaagaag cct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTIFRFADG NSMTTKTNLL PHIPSSDAII SRWRWLAIIV SLVMALLASA FILLLSNAVD REVAQLNTSS ELREQADLVL ISLIDMAVAD RSYAKNHKAE DKARFEHAAR NLDDILEYIE LIVYAHPQWY EWFTSFKKEV EARKAFMNDH MRALDALPDQ SSQPSSSSEI PKIQVARLAQ LMTSFINGDD VVRQKQREGI ALMRAALTLA AIVSVVSTFM SAYFVINRFH RDVRSLKMYQ LLLHSENIAL EARVKERTQE LERARNHAEK ERQRVEMLLQ DVSHRIGNSL GTVSSLLGLQ LNRSDNEEVR SVLGAARDRI QTISTAHRRL RLSDDMETTL LAEFLSSVVH DVELAMPLEL RENITIHTNF KECRLSSRDA TTLGIILGEL LTNSLKHAFP DKRSGDIYIS FGPQKNGQLM LIVEDNGVGM KNQTQEKKTG LGRVVVEQLC MQFGEKPLFE TSALGGTKIV IPLPKLTPNA SKEAYA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgattttc aggttcgccg atgggaattc tatgacaaca aagacgaatt tgctccctca tataccctct tctgatgcaa taatatcgcg ttggcgttgg ttagcgatta ttgtttctct tgttatggct ctgttggctt cggcctttat tttgttactc tcaaacgccg ttgatcgcga agttgcacag ttgaatacaa gttcagaatt gcgtgaacaa gctgatttgg tgcttattag tttgatcgat atggccgtag ctgaccgcag ttatgcaaaa aaccacaagg cagaagataa agcacgcttt gaacatgcag ctcgtaatct tgatgatatt ctcgagtata ttgaacttat tgtttacgca catccgcagt ggtatgaatg gtttacttct tttaaaaaag aggtcgaagc acgcaaagcc tttatgaatg accatatgcg cgctcttgat gcgcttcctg atcaatcttc tcaaccctct tcttcttctg aaatcccaaa aattcaagtg gcgcgtttag cgcaattgat gacaagcttt attaatggcg atgatgtggt gcggcaaaaa cagcgcgagg gaatcgcact gatgcgggct gctttaacat tggctgctat tgtttctgtc gtgagtactt tcatgtcggc ctattttgtc attaatcgtt ttcaccgtga tgtacgctct ttaaaaatgt atcaattgct gcttcatagt gaaaatatag cccttgaagc tcgggtaaaa gaacgcacac aagaattgga aagagcacgc aatcacgccg agaaagaacg acaacgtgtt gaaatgttgt tgcaagatgt gagccatcgt attggcaact ctcttggtac ggtctcttct ttgctagggc tccaattaaa tcgcagtgac aatgaagagg tgcgttctgt tcttggtgca gcacgtgacc gtatccagac aatctccaca gcgcatcgtc gtttgcgttt gagtgatgat atggaaacaa ctttattggc agagtttctc agttcggtag tgcatgatgt tgaattggca atgcctcttg aattgcggga aaatatcacc attcacacga actttaaaga gtgccgattg tcttcaagag atgcgacgac attgggcatt attcttggtg agttgttgac aaactcctta aaacatgcct ttcctgataa acgctctggt gatatttaca tctcatttgg tcctcagaaa aatgggcaac tgatgctgat tgtggaagat aatggtgtgg gcatgaagaa tcaaacgcaa gagaaaaaaa caggtcttgg tcgggtggtc gttgaacagc tttgtatgca gtttggtgaa aaaccgcttt ttgaaacttc tgctttggga ggaacaaaaa ttgtaattcc tttgcccaaa ttaaccccca atgcctcaaa agaagcctat gcttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BaheA.17838.a.A2.GE34610
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMELERARNH AEKERQRVEM LLQDVSHRIG NSLGTVSSLL GLQLNRSDNE EVRSVLGAAR DRIQTISTAH RRLRLSDDME TTLLAEFLSS VVHDVELAMP LELRENITIH TNFKECRLSS RDATTLGIIL GELLTNSLKH AFPDKRSGDI YISFGPQKNG QLMLIVEDNG VGMKNQTQEK KTGLGRVVVE QLCMQFGEKP LFETSALGGT KIVIPLPKLT PNASKEA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaat tggaaagagc acgcaatcac gccgagaaag aacgacaacg tgttgaaatg ttgttgcaag atgtgagcca tcgtattggc aactctcttg gtacggtctc ttctttgcta gggctccaat taaatcgcag tgacaatgaa gaggtgcgtt ctgttcttgg tgcagcacgt gaccgtatcc agacaatctc cacagcgcat cgtcgtttgc gtttgagtga tgatatggaa acaactttat tggcagagtt tctcagttcg gtagtgcatg atgttgaatt ggcaatgcct cttgaattgc gggaaaatat caccattcac acgaacttta aagagtgccg attgtcttca agagatgcga cgacattggg cattattctt ggtgagttgt tgacaaactc cttaaaacat gcctttcctg ataaacgctc tggtgatatt tacatctcat ttggtcctca gaaaaatggg caactgatgc tgattgtgga agataatggt gtgggcatga agaatcaaac gcaagagaaa aaaacaggtc ttggtcgggt ggtcgttgaa cagctttgta tgcagtttgg tgaaaaaccg ctttttgaaa cttctgcttt gggaggaaca aaaattgtaa ttcctttgcc caaattaacc cccaatgcct caaaagaagc ct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMELERARNH AEKERQRVEM LLQDVSHRIG NSLGTVSSLL GLQLNRSDNE EVRSVLGAAR DRIQTISTAH RRLRLSDDME TTLLAEFLSS VVHDVELAMP LELRENITIH TNFKECRLSS RDATTLGIIL GELLTNSLKH AFPDKRSGDI YISFGPQKNG QLMLIVEDNG VGMKNQTQEK KTGLGRVVVE QLCMQFGEKP LFETSALGGT KIVIPLPKLT PNASKEAYA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaattggaa agagcacgca atcacgccga gaaagaacga caacgtgttg aaatgttgtt gcaagatgtg agccatcgta ttggcaactc tcttggtacg gtctcttctt tgctagggct ccaattaaat cgcagtgaca atgaagaggt gcgttctgtt cttggtgcag cacgtgaccg tatccagaca atctccacag cgcatcgtcg tttgcgtttg agtgatgata tggaaacaac tttattggca gagtttctca gttcggtagt gcatgatgtt gaattggcaa tgcctcttga attgcgggaa aatatcacca ttcacacgaa ctttaaagag tgccgattgt cttcaagaga tgcgacgaca ttgggcatta ttcttggtga gttgttgaca aactccttaa aacatgcctt tcctgataaa cgctctggtg atatttacat ctcatttggt cctcagaaaa atgggcaact gatgctgatt gtggaagata atggtgtggg catgaagaat caaacgcaag agaaaaaaac aggtcttggt cgggtggtcg ttgaacagct ttgtatgcag tttggtgaaa aaccgctttt tgaaacttct gctttgggag gaacaaaaat tgtaattcct ttgcccaaat taacccccaa tgcctcaaaa gaagcctatg cttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BaheA.17838.a.A2.PW35220
PURIFICATION DATe: 3/16/2012
CONCENTRATION: 20.69mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 26
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMELERARNH AEKERQRVEM LLQDVSHRIG NSLGTVSSLL GLQLNRSDNE EVRSVLGAAR DRIQTISTAH RRLRLSDDME TTLLAEFLSS VVHDVELAMP LELRENITIH TNFKECRLSS RDATTLGIIL GELLTNSLKH AFPDKRSGDI YISFGPQKNG QLMLIVEDNG VGMKNQTQEK KTGLGRVVVE QLCMQFGEKP LFETSALGGT KIVIPLPKLT PNASKEA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaat tggaaagagc acgcaatcac gccgagaaag aacgacaacg tgttgaaatg ttgttgcaag atgtgagcca tcgtattggc aactctcttg gtacggtctc ttctttgcta gggctccaat taaatcgcag tgacaatgaa gaggtgcgtt ctgttcttgg tgcagcacgt gaccgtatcc agacaatctc cacagcgcat cgtcgtttgc gtttgagtga tgatatggaa acaactttat tggcagagtt tctcagttcg gtagtgcatg atgttgaatt ggcaatgcct cttgaattgc gggaaaatat caccattcac acgaacttta aagagtgccg attgtcttca agagatgcga cgacattggg cattattctt ggtgagttgt tgacaaactc cttaaaacat gcctttcctg ataaacgctc tggtgatatt tacatctcat ttggtcctca gaaaaatggg caactgatgc tgattgtgga agataatggt gtgggcatga agaatcaaac gcaagagaaa aaaacaggtc ttggtcgggt ggtcgttgaa cagctttgta tgcagtttgg tgaaaaaccg ctttttgaaa cttctgcttt gggaggaaca aaaattgtaa ttcctttgcc caaattaacc cccaatgcct caaaagaagc ct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMELERARNH AEKERQRVEM LLQDVSHRIG NSLGTVSSLL GLQLNRSDNE EVRSVLGAAR DRIQTISTAH RRLRLSDDME TTLLAEFLSS VVHDVELAMP LELRENITIH TNFKECRLSS RDATTLGIIL GELLTNSLKH AFPDKRSGDI YISFGPQKNG QLMLIVEDNG VGMKNQTQEK KTGLGRVVVE QLCMQFGEKP LFETSALGGT KIVIPLPKLT PNASKEAYA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAATTGGAA AGAGCACGCA ATCACGCCGA GAAAGAACGA CAACGTGTTG AAATGTTGTT GCAAGATGTG AGCCATCGTA TTGGCAACTC TCTTGGTACG GTCTCTTCTT TGCTAGGGCT CCAATTAAAT CGCAGTGACA ATGAAGAGGT GCGTTCTGTT CTTGGTGCAG CACGTGACCG TATCCAGACA ATCTCCACAG CGCATCGTCG TTTGCGTTTG AGTGATGATA TGGAAACAAC TTTATTGGCA GAGTTTCTCA GTTCGGTAGT GCATGATGTT GAATTGGCAA TGCCTCTTGA ATTGCGGGAA AATATCACCA TTCACACGAA CTTTAAAGAG TGCCGATTGT CTTCAAGAGA TGCGACGACA TTGGGCATTA TTCTTGGTGA GTTGTTGACA AACTCCTTAA AACATGCCTT TCCTGATAAA CGCTCTGGTG ATATTTACAT CTCATTTGGT CCTCAGAAAA ATGGGCAACT GATGCTGATT GTGGAAGATA ATGGTGTGGG CATGAAGAAT CAAACGCAAG AGAAAAAAAC AGGTCTTGGT CGGGTGGTCG TTGAACAGCT TTGTATGCAG TTTGGTGAAA AACCGCTTTT TGAAACTTCT GCTTTGGGAG GAACAAAAAT TGTAATTCCT TTGCCCAAAT TAACCCCCAA TGCCTCAAAA GAAGCCTATG CTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA