BaheA.17837.a

Filamentous hemagglutinin, adhesin-like protein

CENTER ID: BaheA.17837.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.17837.a.A2.GE34612 pectin lyase domain 30 330
BaheA.17837.a.A6.GE33152 Pfam-B_665 1740 2362
BaheA.17837.a.A7.GE33153 Pfam-B_9140 2372 2651
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.774
RefSeq: YP_033538.1
UniProt: A0A0H3LY71

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKKVLFWGL GFSCLLAPSA LQAQISVDAK ANSAHRPDIV AAPNGVPSID IVTPNSSGLS HNKYDDFNIG HVGVIWNNHA QEVGQSQLGG IMPGNPHLRF SGSAKVILNE VTSGKRSALN GPGEVFGKQA DVIIANPNGI SCDGCGFINT PHATLTTGVP EIDVSGFLKG FVVKGGEITF GARGANFFSG KGVVDVVDIV SRTVHFEGPV AGREIGVAAG TGNFDYASRQ MKALTDITGK PEYAIDGSAL GALQADQIKL VATEKGVGVR MRHDMAANAG QLHLSADGKI SLQNAFGHRG VVLKSKSQSV LAKHIASKKH IEIAAKKDVT LETIGADGHF IAEAQEGLLT IAGQATSGGN MQLSSREAIK VSGLGAGADI AFATGGDLTI GGTILSGGNL KAHAGGDIRA HLLAGGVDMA ATGAAGKLVL GSHGGVDLQS VGGIIDAESI YGAGEITLVS HNGVSVSQFL QSHDNVAIHT QPDAGVHFGQ LIAYGRADID GGAVDFSSLM TGGDAVLKVR NLEAGTLMTG GDFVQSSVFG KLILHEKGSL SITAQRGIKV GHIISGENIA LFAGNDIYYD QVIGYGSTTL TSGSGGISVE NVLSAMGDVR LTANTLDLSK NCSHIYTPQT LYLKGDHIDV SGSELTYGGL DFQSTNALDI QHARLQAVTD KGGSGDIVFR APGIMTDKET SVLAARDFTI KTGKLHNSGQ LAAGQNLAFS VTGDATNSKT GLVYVKGNGA LKVDGALLND FGAIVAEGDL SFTNLGGTGK SLSLVNKAGL IQAGGNLNIQ TKTLQNEADS IPEMKEKKEY SDLSFQRPEG ADQLSDGMLY QDGPNLWGKG HEHQSHTGPF KGHVKIFLDT PLWNSKEGTY GTATLKDGSV YKAFTWEFEP VYDQKSVKRY RWDNWSHMTE ETVTQAFSHK PAVQGMIQST GDLIIHADNI ENNYSIMRAG GNADIYVSVL TNVGATAYKN TYLGCKVHTD AYCYGYKADG SRDVSLDIAN GTFRQTGSEV LDTVSGLVQA GGTLNLVVNT LNNTAAEGSI TGDAHFEAKA VGGNPLEALS GLTGAGALFT PKVDLNNSGE PSEGLPLPKP QSGGVGGTLP QQNFLYETRA EFLDVGKFYG SAYFLNRIGY NPDREIFFLG DAYFEKQLVE KQMRDLVGQG LGKGSFIPGS DAIEQVKTLL DVGADYVKAN NLVFGEALSE EQLASLEAPM VIYVRQRIKG MDVYAPVLYI PEKDSASFVS AGALIMGDDV NITSQNTSTS TVTNSGRIAA NHQLHVHGGD ILSQGGHFAA GSDAVLLAEN NIHLDAGRTM VDGVETVLNT NALSAGGNAT VLAKKDMTAS GVKISTGGDL AMATEQGNLT IGAIKTTDHD AQGNASMHQQ SEVNSGGSTT LSSGKDLNIL GSEVQTKDNL FLQAKDTVSL DATRNSANSY RGDHTSHVAV HNGSYLSSGK ETTVLSGKDI RVFASDIDAK GNVALGAQGN ITIDTKSDEM EYHLQGTNLK VDMQASRAVG SSINAGGDFT AIAGQDGTPH DLTITGSAVT ADGKVELKAS NAILITNAEN SLQYEMSYHK KGGVFSSSKS QHNKIDAAQV VGSLISGGKG VALDSANNTE IVASIFTAGK PGEKTPEEQA KADMTIHSGG NILIKGAQEK YNQQQQSSTS SVLHEKSSDN SEAHTTTVSS ILGATGNIVL DAQGDATLTA SHMIANEDIN VAGQSVTIDG MTDHHSSHSE THETGFGVGS GKGFVSIYGS ESKTENEESF EHQGSSLNGK NITITAKKED VNVVGSDFSA GEDIKLSAVH NVNVLPGHKS HSASTQEERS GFGFQFEKNR SGASVGVGVA SAKDTGDQWE KSNTPSNFKA GRDVQINAGN DVNLQATNVS ANRDVNIDAG NNITLETSYD ISKAEEKHEK SFAGVTGSVN VGILGTVQDI RDAVKRAKNK DNKHHIINGL LGGLKGYDLY NKGKGLYNGM QGGATKDDLL NMADVSTSIT VDFKAEKAEA FSHTFTAVTD SIEAGRAINI HANKGSIHGI GADLIAGTNS LNNDEQSGNI NLEAGQDITF ESAQNRQNTQ NHSQNISVNV GVGYGYGSGG SGWRGNASFG KGKGSSEQVH QKNSHIIGTG TVHSNSGGNT TLAGGVVSAN RVEMGVGGDF TITSHSDTGQ SSSKQNSVSV GFNGGKKDDA ATTNLALNKD KTSSDYHSVV EHSGIKAGDG GFEINVKDTT TLTGGIIDSS ASADKNKLTT GTITTSDIAN SAKAQASSQG LSLATGGPMS QGKYGLAKNI AKNVLDHAKA KDSQEGQTKA AISNGTIILT DEVEQKALTG QDVEQVIASL NRDTANAHKG VQQLDVTKLE QIVHENREMA TQLLEEGFKV SNEAYKTMFI KEHPIAVVER DEQGHIIYKT DENGVPIRDA RGQKIPKSHS LTAEEKEHLQ AGSDGKVHVS FNGIFIPPEE AAVYAEQHAV NKNEPLYFVV FPQADTALSE LLVAGYQKFL ENNFWGLTNS TQEAKDLMSR YGNTGLELYA HSRGSMTLGN MLYSFQQQGV HGIANKNTTI NLYGPAFNVL VASDLLGYVS DGKQTTVGFD GHRYDFVSRW IGGNSYTYKT ILADSNAWKE WWRVVTNPIS SHTCLGDASD ACKKRYGSSH REQFPLSKTR SKK
NT Sequence
atgctcaaga aggttttatt ttgggggctt ggtttttctt gtttgttagc accttcagcg ttacaggcgc aaatttctgt tgatgcgaag gcgaattctg cccatcgtcc agatattgtg gcagccccca atggggttcc ttccattgat attgttaccc ccaacagcag cggtttatcg cacaataaat atgatgattt taatattggc catgtaggtg tgatttggaa caatcatgcg caagaagtgg gacaatcgca attggggggc attatgcctg gcaatccaca tttgcgtttt tctggttcag cgaaggttat tttaaatgaa gtaacaagcg gaaaacgcag tgcgttgaat ggcccaggag aagtttttgg aaaacaagcc gatgtgatta tagccaaccc caatggcata agctgtgatg gttgtgggtt tatcaatacg ccccatgcga cattaaccac cggtgttcca gaaattgatg tttctggttt tttgaaaggt tttgtggtga aaggtgggga gatcactttt ggtgcaaggg gtgcgaattt tttttcaggc aaaggggtgg ttgatgttgt cgatattgtt tcacgcactg tgcattttga aggtcctgtt gcgggcaggg aaattggagt tgcggctggt acgggaaatt ttgattatgc ttctcgccaa atgaaggcac ttaccgatat tactggtaag ccggaatatg cgatagatgg ttctgctttg ggggctctcc aggcggatca gattaaactt gttgcgacag aaaaaggtgt tggggttcgc atgcgccatg atatggcggc caatgcgggg cagttacatc tttctgcgga tggaaaaatc tcactacaga atgcctttgg tcataggggt gttgttctga aatcaaaaag ccagagtgtg ttggcaaaac acattgcatc caaaaagcat attgagattg cggcaaaaaa agatgtgacg ttagaaacca ttggggcaga tggtcatttt atagcagagg cacaagaagg gcttttaacc attgccgggc aagcgacctc tggaggcaat atgcagctgt cttcgcgcga agccatcaaa gtctctgggc taggggcagg ggctgatata gcgtttgcaa cgggcggtga tctgaccatt ggtggcacta ttttgtctgg gggcaattta aaagcacatg ctggtggtga tatcagggcg catcttttag ccggtggggt tgatatggca gcaacggggg ctgctgggaa gcttgttttg ggcagccatg ggggtgttga tctccaaagt gtgggaggca ttattgatgc ggaaagcatt tatggagcgg gagagatcac cctggtttct cataacgggg tttcggtttc tcaatttctt caatctcatg ataatgttgc tattcatacc caaccggatg ctggagttca ttttggacaa ctgattgctt atggaagggc agacattgat ggtggagcgg ttgatttttc ttctctgatg acgggtggag atgctgtttt aaaggtcagg aaccttgagg ctggcacact gatgacaggg ggagattttg tccaatcgag tgtttttggc aaacttattt tgcatgagaa gggctcgctt tcgattacgg cacaaagggg gataaaggtt ggacatatta tcagtggtga aaatattgcc ctttttgctg gcaacgacat ttattatgat caggttatcg gctatggcag cacaacactg acatcgggat ctggagggat tagtgttgag aatgtgctgt ctgccatggg ggatgtgagg ttgacagcaa acactctcga tttaagcaag aattgttctc atatttacac gcctcaaacg ctgtatttaa agggggatca tattgatgtt tcggggagcg aattgactta tggcggttta gactttcaaa gcaccaatgc ccttgatatt cagcatgcac ggctgcaagc agtgacagat aaggggggga gtggagatat tgtttttaga gctccaggga ttatgacgga taaggaaaca tcggttttag cggcacgaga ttttaccatc aaaacaggga agcttcacaa tagtggtcaa ttggcagcgg ggcagaattt agccttcagt gtgacaggtg atgcgaccaa cagcaaaacg ggtttagtct atgtgaaggg caatggtgcg ctcaaagttg atggggcttt gttgaatgat tttggtgcca ttgtggcaga gggtgattta tctttcacca atttgggggg cacaggaaag agcctttctc ttgtcaacaa agcaggtttg attcaggcag gtggaaactt aaacatccaa accaaaacat tgcaaaatga agccgatagc ataccggaaa tgaaggaaaa aaaggaatat agcgatcttt catttcaaag accagagggg gctgatcaac tcagtgatgg gatgttatat caagatgggc ccaatctttg gggaaagggg cacgaacatc aaagtcatac gggacctttt aaagggcacg taaaaatttt cttagatact ccactttgga atagcaaaga aggaacgtat ggtacggcaa ctttaaaaga tggatcagtc tataaggcat ttacttggga atttgagcct gtctatgatc aaaagtctgt aaaacggtat cgatgggata actggtcgca tatgacagag gagactgtca cgcaggcgtt ttcgcataaa ccggcggttc aggggatgat tcagtctacg ggtgatctta ttattcacgc tgataacatt gagaataact atagcatcat gagagctggg ggcaatgcgg atatctatgt gagtgtgctg accaatgtag gggcaacagc ctataaaaat acttatcttg gttgtaaggt ccatacggat gcttattgtt atggctataa agccgatggt agccgtgatg tttctttaga tatagccaat ggcacgtttc gtcagactgg ctcagaagtt ctcgatacgg tttctggtct tgttcaagcg ggtggcactt taaatctagt ggtcaatacc ctcaacaata cggcagccga agggtccatt acgggagatg cgcattttga agccaaagcg gttggaggta atccgctgga ggcgctgagt ggtttgaccg gggctggtgc tctctttaca ccaaaagtgg atctcaataa ttcaggggaa ccttctgagg ggcttccttt gccaaagccc caatcgggcg gtgttggggg cacgctacct caacagaatt tcctttatga aacgcgggcg gaattccttg atgttggtaa attttatgga tcagcctatt tcttgaacag aattggctac aatcctgata gggagatttt tttcttaggg gatgcttatt ttgaaaagca attggtagaa aaacaaatgc gtgatcttgt gggtcaaggt ttaggcaaag gctcctttat tccaggaagt gatgccattg aacaagtcaa aaccttgcta gatgtagggg cagactatgt aaaagccaac aatcttgtct ttggagaagc tttaagtgaa gaacaattgg caagtttaga agcgcccatg gtgatttatg tgcgtcaaag aataaagggc atggatgttt atgctcccgt tctttacatc cctgaaaaag atagcgcctc ctttgtttct gctggggctt tgatcatggg cgatgatgtc aatatcacca gccaaaatac aagcacttca acggttacca attcagggcg tattgcagca aatcaccagt tgcatgtgca tggcggggat attctcagtc aaggagggca ttttgctgcg gggagtgatg ctgttttgct tgcggagaac aatattcatt tggatgcagg gcgcacaatg gttgacggtg tggaaactgt tttaaacacc aatgcgcttt ctgctggggg caatgcgaca gttcttgcta agaaagatat gacagcctct ggggtaaaga tcagcacagg gggtgatctt gccatggcca cagagcaggg caatttgaca atcggtgcca taaaaactac cgaccatgat gcgcagggta atgcatccat gcatcaacaa tcagaagtta attctggagg ctcaacaacg ctttcctctg gaaaggatct gaatattctg ggctctgaag ttcaaacgaa ggataatctt ttcttacaag caaaagacac tgtttcgctt gatgccacgc gcaacagtgc caatagctat cggggggatc acacctccca tgttgctgtg cataatggct cttatttaag ttctggaaaa gagaccactg ttctctctgg aaaagatatc cgtgtttttg cgtccgatat cgatgcgaag ggcaatgttg ctcttggtgc tcaagggaat atcacgatag ataccaaatc tgatgagatg gagtatcatc ttcagggtac aaatcttaaa gtggatatgc aagcctcgcg agccgttggc tcttcgatca atgctggggg tgattttact gctattgcag gccaagatgg cacaccacat gatctcacca tcacgggcag tgctgttacg gctgatggca aggtagagct gaaagccagc aatgctattc tcatcaccaa tgcggaaaat agcttacagt atgagatgtc gtatcataaa aagggtgggg tgttcagtag cagcaaatcc cagcataaca agattgatgc ggctcaagtt gtgggctcac tcatatctgg gggcaaaggt gttgctcttg actcagcaaa caacacggag attgtagcct cgatattcac agccggtaag ccaggggaga aaacgccaga agagcaggcc aaagctgata tgaccattca ctctggtgga aatattctca tcaaaggggc gcaagaaaaa tacaaccagc aacaacaatc gtcgacaagc agtgttttgc atgagaaatc ttccgataat tctgaagcgc acactacaac ggtttcctct atccttggag cgacgggcaa tattgtgtta gacgcacaag gggatgccac cctcacggct tctcatatga ttgccaatga agatattaac gtggcaggac aaagcgtgac gattgatggc atgacagatc accatagcag ccattcagaa acccatgaaa cgggttttgg tgtgggatca ggcaagggtt ttgtttcgat ctacgggagt gaatcaaaga cagagaatga agaaagtttt gaacatcaag gctcttcgct caatgggaaa aatatcacca tcaccgccaa aaaagaagat gtgaatgtgg tgggttctga ctttagcgca ggggaagata ttaagctttc ggcagtccat aatgtgaatg ttttgcctgg tcataagagt catagcgcga gcacacagga agaacgctca ggttttggct ttcagtttga aaagaaccgc agtggtgcct ctgtgggtgt gggggttgcg agcgccaaag atacgggcga tcaatgggaa aagtccaata caccatccaa tttcaaagcg ggcagagatg tgcagatcaa tgccggcaat gatgtgaatt tacaagcgac aaatgtttcg gcaaatcgtg atgtcaacat tgatgctggc aataatatca cgcttgagac aagctatgat atctctaagg cagaagaaaa gcatgaaaaa tcctttgctg gtgtgacagg ttctgtcaat gttggtattc ttggtacggt acaagatata agggatgcag taaagcgagc taaaaataag gataacaaac accacattat taatggtttg cttggcggtt taaaaggtta tgatctctat aacaaaggca aaggtcttta caatgggatg cagggtggag caaccaaaga tgaccttctt aatatggcgg atgtttcaac cagcattacg gtagacttta aagctgaaaa ggcagaggca ttttctcaca ccttcaccgc agtaacagat agcatagaag cagggcgtgc cattaacata catgccaaca aaggcagcat ccatggtatt ggtgctgatc ttatcgctgg cacaaactct ctgaacaatg atgaacagag cggaaatatt aatttggaag caggacaaga tatcaccttt gaaagtgcgc aaaataggca aaacacgcaa aaccattcgc aaaatatttc tgtaaatgtt ggtgttggct acggttatgg gagtggcggt tcaggctgga ggggcaatgc ttcttttgga aaaggaaaag gctccagtga acaggtacac cagaagaaca gccatattat aggcacgggc actgttcata gcaacagtgg gggaaacacc acactagcgg ggggtgtggt ttctgcaaac cgtgtagaga tgggtgttgg tggtgatttc accattacaa gccacagtga tacgggacaa agttccagca agcaaaactc tgtttctgtt ggctttaatg gtggaaaaaa ggatgatgcg gcgacaacca atcttgcctt gaacaaggat aaaacctcta gcgattatca cagtgttgtg gaacactccg gcatcaaagc gggtgatggc ggttttgaga tcaacgttaa agatacaaca accctgaccg gggggattat cgacagcagt gcatcagccg acaaaaacaa actgaccacc ggaaccatca ccacgagtga tattgccaac agtgctaaag cgcaagccag cagccagggt ttaagccttg ctacgggtgg tcccatgtct cagggcaaat acggtcttgc gaaaaacatc gccaaaaatg tcttagatca tgcaaaagcc aaagattcac aggaagggca aaccaaagct gctatcagca atggcaccat catcctgaca gatgaggttg agcaaaaggc attgacagga caagatgtgg aacaagtaat cgcctccctt aaccgcgata ctgccaacgc ccataagggg gtacaacagc ttgatgttac caagctggaa caaattgtcc atgaaaaccg tgaaatggcg acacaattgc tggaagaagg gtttaaagtt agcaatgaag cttacaaaac catgtttatc aaagaacatc ccatcgccgt ggtggagcgt gatgaacagg gacatatcat ctataagaca gatgaaaatg gggtacctat aagagacgct cgtggacaaa aaattccgaa atctcattct ttaacggctg aggagaagga acatttgcaa gctggttctg atggcaaggt gcatgtgtcg ttcaatggta tctttatacc accagaggaa gctgctgttt atgccgagca acatgctgtt aataaaaatg aaccgcttta ttttgtggtg tttccacaag ccgatacggc tctctcagaa cttctggtag cggggtatca gaagtttctg gaaaataact tttggggttt gaccaattca acacaagaag caaaagatct gatgtctcgc tatggtaata cagggttaga actttatgcc catagtcgcg gtagcatgac attggggaat atgctgtatt cttttcaaca acaaggtgtg cacgggatag ctaacaaaaa cacgactatc aatctctatg gaccagcctt taatgtttta gttgcatccg atctgttagg ctatgtgagt gacggcaaac aaaccactgt tggttttgat gggcatagat atgattttgt cagccgatgg attggcggta atagctatac ttacaagaca attcttgcag acagtaatgc ttggaaagaa tggtggagag tggttacaaa tcccataagt tcccatacct gtcttggaga tgcgagtgac gcgtgtaaaa agcgctatgg ctcatctcat cgtgaacaat ttcctttaag taaaacaagg agtaaaaaat ga
Details for BaheA.17837.a.A2.GE34612
HARVESTED ON: 1/31/2012
SEQUENCED ON: 2/3/2012
EXPECTED MW: 33kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 96
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKANSAHRP DIVAAPNGVP SIDIVTPNSS GLSHNKYDDF NIGHVGVIWN NHAQEVGQSQ LGGIMPGNPH LRFSGSAKVI LNEVTSGKRS ALNGPGEVFG KQADVIIANP NGISCDGCGF INTPHATLTT GVPEIDVSGF LKGFVVKGGE ITFGARGANF FSGKGVVDVV DIVSRTVHFE GPVAGREIGV AAGTGNFDYA SRXMKALTDI TGKPEYAIDG SALGALXADQ IKLVATEKGV GVRMRHDMAA NAGQLHLSAD GKISLQNAFG HRGVXLKSKS XXXXAXXLXX K
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaagg cgaattctgc ccatcgtcca gatattgtgg cagcccccaa tggggttcct tccattgata ttgttacccc caacagcagc ggtttatcgc acaataaata tgatgatttt aatattggcc atgtaggtgt gatttggaac aatcatgcgc aagaagtggg acagtcgcaa ttggggggca ttatgcctgg caatccacat ttgcgttttt ctggttcagc gaaggttatt ttaaatgaag taacaagcgg aaaacgcagt gcgttgaatg gcccaggaga agtttttgga aaacaagccg atgtgattat agccaacccc aatggcataa gctgtgatgg ttgtgggttt atcaatacgc cccatgcgac attaaccacc ggtgttccag aaattgatgt ttctggtttt ttgaaaggtt ttgtggtgaa aggtggggag atcacttttg gtgcaagggg tgcgaatttt ttttcaggca aaggggtggt tgatgttgtc gatattgttt cacgcactgt gcattttgaa ggtcctgttg cgggcaggga aattggagtt gcggctggta cgggaaattt tgattatgct tctcgccnaa tgaaggcact taccgatatt actggtaagc cggaatatgc gatagatggt tctgctttgg gggctctccn ggcggatcag attaaacttg ttgcgacaga aaaaggtgtt ggggttcgca tgcgccatga tatggcggcc aatgcggggc agttacatct ttctgcggat ggaaaaatct cactacagaa tgcctttggt cataggggtg ttgntctgaa atcaaaaagc cngantgngn tggcaaannc attgcnnnca aaagca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKANSAHRP DIVAAPNGVP SIDIVTPNSS GLSHNKYDDF NIGHVGVIWN NHAQEVGQSQ LGGIMPGNPH LRFSGSAKVI LNEVTSGKRS ALNGPGEVFG KQADVIIANP NGISCDGCGF INTPHATLTT GVPEIDVSGF LKGFVVKGGE ITFGARGANF FSGKGVVDVV DIVSRTVHFE GPVAGREIGV AAGTGNFDYA SRQMKALTDI TGKPEYAIDG SALGALQADQ IKLVATEKGV GVRMRHDMAA NAGQLHLSAD GKISLQNAFG HRGVVLKSKS QSVLAKHIAS KKHIEIAAKK DVT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaggcgaat tctgcccatc gtccagatat tgtggcagcc cccaatgggg ttccttccat tgatattgtt acccccaaca gcagcggttt atcgcacaat aaatatgatg attttaatat tggccatgta ggtgtgattt ggaacaatca tgcgcaagaa gtgggacaat cgcaattggg gggcattatg cctggcaatc cacatttgcg tttttctggt tcagcgaagg ttattttaaa tgaagtaaca agcggaaaac gcagtgcgtt gaatggccca ggagaagttt ttggaaaaca agccgatgtg attatagcca accccaatgg cataagctgt gatggttgtg ggtttatcaa tacgccccat gcgacattaa ccaccggtgt tccagaaatt gatgtttctg gttttttgaa aggttttgtg gtgaaaggtg gggagatcac ttttggtgca aggggtgcga attttttttc aggcaaaggg gtggttgatg ttgtcgatat tgtttcacgc actgtgcatt ttgaaggtcc tgttgcgggc agggaaattg gagttgcggc tggtacggga aattttgatt atgcttctcg ccaaatgaag gcacttaccg atattactgg taagccggaa tatgcgatag atggttctgc tttgggggct ctccaggcgg atcagattaa acttgttgcg acagaaaaag gtgttggggt tcgcatgcgc catgatatgg cggccaatgc ggggcagtta catctttctg cggatggaaa aatctcacta cagaatgcct ttggtcatag gggtgttgtt ctgaaatcaa aaagccagag tgtgttggca aaacacattg catccaaaaa gcatattgag attgcggcaa aaaaagatgt gacgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BaheA.17837.a.A6.GE33152
HARVESTED ON: 8/19/2011
SEQUENCED ON: 10/23/2013
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 45
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGKGFVSI YGSESKTENE ESFEHQGSSL NGKNITITAK KEDVNVVGSD FSAGEDIKLS AVHNVNVLPG HKSHSASTQE ERSGFGFQFE KNRSGASVGV GVASAKDTGD QWEKSNTPSN FKAGRDVQIN AGNDVNLQAT NVSANRDVNI DAGNNITLET SYDISKAEEK HEKSFAGVTG SVNVGILGTV QDIRDAVKRA KNKDNKHHII NGLLGGLKGY DLYNKGKGLY NGMXGGATKD DLLNMAXVST SITVDFKAEK XXAFSHTXTA VTDSI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcag gcaagggttt tgtttcgatc tacgggagtg aatcaaagac agagaatgaa gaaagttttg aacatcaagg ctcttcgctc aatgggaaaa atatcaccat caccgccaaa aaagaagatg tgaatgtggt gggttctgac tttagcgcag gggaagatat taagctttcg gcagtccata atgtgaatgt tttgcctggt cataagagtc atagcgcgag cacacaggaa gaacgctcag gttttggctt tcagtttgaa aagaaccgca gtggtgcctc tgtgggtgtg ggggttgcga gcgccaaaga tacgggcgat caatgggaaa agtccaatac accatccaat ttcaaagcgg gcagagatgt gcagatcaat gccggcaatg atgtgaattt acaagcgaca aatgtttcgg caaatcgtga tgtcaacatt gatgctggca ataatatcac gcttgagaca agctatgata tctctaaggc agaagaaaag catgaaaaat cctttgctgg tgtgacaggt tctgtcaatg ttggtattct tggtacggta caagatataa gggatgcagt aaagcgagct aaaaataagg ataacaaaca ccacattatt aatggtttgc ttggcggttt aaaaggttat gatctctata acaaaggcaa aggtctttac aatgggatgc anggtggagc aaccaaagat gaccttctta atatggcgga ngtttcaacc agcattacgg tagactttaa agctgaaaag gnagangcat tttctcacac cntcaccgca gtaacagata gcatana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGKGFVSI YGSESKTENE ESFEHQGSSL NGKNITITAK KEDVNVVGSD FSAGEDIKLS AVHNVNVLPG HKSHSASTQE ERSGFGFQFE KNRSGASVGV GVASAKDTGD QWEKSNTPSN FKAGRDVQIN AGNDVNLQAT NVSANRDVNI DAGNNITLET SYDISKAEEK HEKSFAGVTG SVNVGILGTV QDIRDAVKRA KNKDNKHHII NGLLGGLKGY DLYNKGKGLY NGMQGGATKD DLLNMADVST SITVDFKAEK AEAFSHTFTA VTDSIEAGRA INIHANKGSI HGIGADLIAG TNSLNNDEQS GNINLEAGQD ITFESAQNRQ NTQNHSQNIS VNVGVGYGYG SGGSGWRGNA SFGKGKGSSE QVHQKNSHII GTGTVHSNSG GNTTLAGGVV SANRVEMGVG GDFTITSHSD TGQSSSKQNS VSVGFNGGKK DDAATTNLAL NKDKTSSDYH SVVEHSGIKA GDGGFEINVK DTTTLTGGII DSSASADKNK LTTGTITTSD IANSAKAQAS SQGLSLATGG PMSQGKYGLA KNIAKNVLDH AKAKDSQEGQ TKAAISNGTI ILTDEVEQKA LTGQDVEQVI ASLNRDTANA HKGVQQLDVT KLEQIVHENR EMATQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcaggcaag ggttttgttt cgatctacgg gagtgaatca aagacagaga atgaagaaag ttttgaacat caaggctctt cgctcaatgg gaaaaatatc accatcaccg ccaaaaaaga agatgtgaat gtggtgggtt ctgactttag cgcaggggaa gatattaagc tttcggcagt ccataatgtg aatgttttgc ctggtcataa gagtcatagc gcgagcacac aggaagaacg ctcaggtttt ggctttcagt ttgaaaagaa ccgcagtggt gcctctgtgg gtgtgggggt tgcgagcgcc aaagatacgg gcgatcaatg ggaaaagtcc aatacaccat ccaatttcaa agcgggcaga gatgtgcaga tcaatgccgg caatgatgtg aatttacaag cgacaaatgt ttcggcaaat cgtgatgtca acattgatgc tggcaataat atcacgcttg agacaagcta tgatatctct aaggcagaag aaaagcatga aaaatccttt gctggtgtga caggttctgt caatgttggt attcttggta cggtacaaga tataagggat gcagtaaagc gagctaaaaa taaggataac aaacaccaca ttattaatgg tttgcttggc ggtttaaaag gttatgatct ctataacaaa ggcaaaggtc tttacaatgg gatgcagggt ggagcaacca aagatgacct tcttaatatg gcggatgttt caaccagcat tacggtagac tttaaagctg aaaaggcaga ggcattttct cacaccttca ccgcagtaac agatagcata gaagcagggc gtgccattaa catacatgcc aacaaaggca gcatccatgg tattggtgct gatcttatcg ctggcacaaa ctctctgaac aatgatgaac agagcggaaa tattaatttg gaagcaggac aagatatcac ctttgaaagt gcgcaaaata ggcaaaacac gcaaaaccat tcgcaaaata tttctgtaaa tgttggtgtt ggctacggtt atgggagtgg cggttcaggc tggaggggca atgcttcttt tggaaaagga aaaggctcca gtgaacaggt acaccagaag aacagccata ttataggcac gggcactgtt catagcaaca gtgggggaaa caccacacta gcggggggtg tggtttctgc aaaccgtgta gagatgggtg ttggtggtga tttcaccatt acaagccaca gtgatacggg acaaagttcc agcaagcaaa actctgtttc tgttggcttt aatggtggaa aaaaggatga tgcggcgaca accaatcttg ccttgaacaa ggataaaacc tctagcgatt atcacagtgt tgtggaacac tccggcatca aagcgggtga tggcggtttt gagatcaacg ttaaagatac aacaaccctg accgggggga ttatcgacag cagtgcatca gccgacaaaa acaaactgac caccggaacc atcaccacga gtgatattgc caacagtgct aaagcgcaag ccagcagcca gggtttaagc cttgctacgg gtggtcccat gtctcagggc aaatacggtc ttgcgaaaaa catcgccaaa aatgtcttag atcatgcaaa agccaaagat tcacaggaag ggcaaaccaa agctgctatc agcaatggca ccatcatcct gacagatgag gttgagcaaa aggcattgac aggacaagat gtggaacaag taatcgcctc ccttaaccgc gatactgcca acgcccataa gggggtacaa cagcttgatg ttaccaagct ggaacaaatt gtccatgaaa accgtgaaat ggcgacacaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BaheA.17837.a.A7.GE33153
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 105
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNEAYKTMF IKEHPIAVVE RDEQGHIIYK TDENGVPIRD ARGQKIPKSH SLTAEEKEHL QAGSDGKVHV SFNGIFIPPE EAAVYAEQHA VNKNEPLYFV VFPQADTALS ELLVAGYQKF LENNFWGLTN STQEAKDLMS RYGNTGLELY AHSRGSMTLG NMLYSFQQQG VHGIANKNTT INLYGPAFNV LVASDLLGYV SDGXQTTVGF DGHRYDFVSR WIGGNSYTYX TILADSNAWK EWWRVVTNPI SSHTCLGDAS DACKKRYGSS HREXFPXSKX XVNKQHEQVL QPSFSRIXC
Validated NT Sequence
ttgtttaact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaatgaa gcttacaaaa ccatgtttat caaagaacat cccatcgccg tggtggagcg tgatgaacag ggacatatca tctataagac agatgaaaat ggggtaccta taagagacgc tcgtggacaa aaaattccga aatctcattc tttaacggct gaggagaagg aacatttgca agctggttct gatggcaagg tgcatgtgtc gttcaatggt atctttatac caccagagga agctgctgtt tatgccgagc aacatgctgt taataaaaat gaaccgcttt attttgtggt gtttccacaa gccgatacgg ctctctcaga acttctggta gcggggtatc agaagtttct ggaaaataac ttttggggtt tgaccaattc aacacaagaa gcaaaagatc tgatgtctcg ctatggtaat acagggttag aactttatgc ccatagtcgc ggtagcatga cattggggaa tatgctgtat tcttttcaac aacaaggtgt gcacgggata gctaacaaaa acacgactat caatctctat ggaccagcct ttaatgtttt agttgcatcc gatctgttag gctatgtgag tgacggcnaa caaaccactg ttggttttga tgggcataga tatgattttg tcagccgatg gattggcggt aatagctata cttacnagac aattcttgca gacagtaatg cttggaaaga atggtggaga gtggttacaa atcccataag ttcccatacc tgtcttggag atgcgagtga cgcgtgtaaa aagcgctatg gctcatctca tcgtgaacna tttcctttna gtaaannnnn agttaataaa cagcacgaac aagttctgca gccnagcttc tcnaggatcc nntgctaaca aancccgaaa nnaanctgan ttggctgctg cnncnctnnn nnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNEAYKTMF IKEHPIAVVE RDEQGHIIYK TDENGVPIRD ARGQKIPKSH SLTAEEKEHL QAGSDGKVHV SFNGIFIPPE EAAVYAEQHA VNKNEPLYFV VFPQADTALS ELLVAGYQKF LENNFWGLTN STQEAKDLMS RYGNTGLELY AHSRGSMTLG NMLYSFQQQG VHGIANKNTT INLYGPAFNV LVASDLLGYV SDGKQTTVGF DGHRYDFVSR WIGGNSYTYK TILADSNAWK EWWRVVTNPI SSHTCLGDAS DACKKRYGSS HREQFPLSKT RS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatgaagct tacaaaacca tgtttatcaa agaacatccc atcgccgtgg tggagcgtga tgaacaggga catatcatct ataagacaga tgaaaatggg gtacctataa gagacgctcg tggacaaaaa attccgaaat ctcattcttt aacggctgag gagaaggaac atttgcaagc tggttctgat ggcaaggtgc atgtgtcgtt caatggtatc tttataccac cagaggaagc tgctgtttat gccgagcaac atgctgttaa taaaaatgaa ccgctttatt ttgtggtgtt tccacaagcc gatacggctc tctcagaact tctggtagcg gggtatcaga agtttctgga aaataacttt tggggtttga ccaattcaac acaagaagca aaagatctga tgtctcgcta tggtaataca gggttagaac tttatgccca tagtcgcggt agcatgacat tggggaatat gctgtattct tttcaacaac aaggtgtgca cgggatagct aacaaaaaca cgactatcaa tctctatgga ccagccttta atgttttagt tgcatccgat ctgttaggct atgtgagtga cggcaaacaa accactgttg gttttgatgg gcatagatat gattttgtca gccgatggat tggcggtaat agctatactt acaagacaat tcttgcagac agtaatgctt ggaaagaatg gtggagagtg gttacaaatc ccataagttc ccatacctgt cttggagatg cgagtgacgc gtgtaaaaag cgctatggct catctcatcg tgaacaattt cctttaagta aaacaaggag ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa