BaheA.17833.a

Surface protein/Bartonella adhesin

CENTER ID: BaheA.17833.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.17833.a.A2.GE33143 head domain 1 Ylhead-HIN1-neck 161 376
BaheA.17833.a.A4.GE33144 head domain 3 DALL3-neck-FGG 504 627
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.178
RefSeq: YP_033006.1
UniProt: A0A0H3LX70

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKLSVTSKR QYNLYASPIS RRLSLLMKLS LETVTVMFLL GASPVLASNL ALTGAKNLSQ NSPGVNYSKG SHGSIVLSGD DDFCGADYVL GRGGNSTVRN GIPISVEEEY ERFVKQKLMN NATSPYSQSS EQQVWTGDGL TSKGSGYMGG KSTDGDKNIL PEAYGIYSFA TGCGSSAQGN YSVAFGANAT ALTGGSQAFG VAALASGRVS VAIGVGSEAT GEAGVSLGGL SKAAGARSVA IGTRANAYGE ESIAIGGGLK QGSDNKIGSA VAQGLKAISI GSDSVGFQHY AVAIGAKSRA LLLKSVALGS YSVADVDAGV RGYDPVEDEP SKNVSFVWKS SVGAVSVGNR KEGLTRQIIG VAAGTEDTDA VNVAQLKALR GMISEKGGWN LTVNNDNNTV VSSGGALDLS SGSKNLKIAK DGKKNNVTFD VARDLTLKSI KLDGVTLNET GLFIANGPQI TASGINAGSQ KITGVAEGTD ANDAVNFGQL KKIETEVKEQ VAASGFVKQD SDTKYLTIGK DTDGDTINIA NNKSDKRTLM GIKEGDISKD SSEAITGSQL FTTNQNVKTV SDNLQTAATN IAKTFGGDAK YEDGEWTAPT FKVKTVTGEG KEEEKTYQNV ADALAGVGSS ITNVQNKVTE QVNNAITKVE GDALLWSDEA NAFVARHEKS KLEKGASKAT QENSKITYLL DGDVSKDSTD AITGKQLYSL GDKIASYLGG NAKYENGEWT APTFKVKTVK EDGKEEEQTY HNVAAAFEGV GTSFTNVKNE ITKQINHLQS DDSAVVHYDK DDKNGSINYA SVTLGKGKDS AAVTLHNVAA GNIAKDSHDA INGSQIYSLN EQLATYFGGG AGYNKEGKWT APTFTVKTVK EDGEEEEKTY QNVAEALTGV GTSFTNIKSE ITKQIANEIS NVTGDSLVKK DLDTNLITIG KEVAGTEINI ASVSKADRTL SGVKEAVKDN EAVNKGQLDK GLKHLSDSLQ SEDSAVVHYD KKTDETGGIN YTSVTLGGKD KTPVALHNVA DGSISKDSHD AINGGQIHTI GEDVAKFLGG AASFNNGAFT GPTYKLSNID AKGDVQQSEF KDIGSAFAGL DTNIKNVNNN VTNKFNELTQ NITNVTQQVK GDALLWSDEA NAFVARHEKS KLGKGASKAT QENSKITYLL DGDVSKDSTD AITGKQLYSL GDKIASYLGG NAKYEDGEWT APTFKVKTVK EDGKEEEKTY QNVAEALTGV GTSFTNVKNE ITKQINHLQS DDSAVVHYDK NKDETGGINY ASVTLGKGKD SAAVTLHNVA DGSISKDSRD AINGSQIYSL NEQLATYFGG GAKYENGQWT APIFKVKTVK EDGEEEEKTY QNVAEALTGV GTSFTNIKSE ITKQIANEIS SVTGDSLVKK DLATNLITIG KEVAGTEINI ASVSKADRTL SGVKEAVKDN EAVNKGQLDT NIKKVEDKLT EAVGKVTQQV KGDALLWSNE DNAFVADHGK DSAKTKSKIT HLLDGNIASG STDAVTGGQL YSLNEQLATY FGGGAKYENG QWTAPTFKVK TVNGEGKEEE QTYQNVAEAL TGVGASFMNV QNKITNEITN QVNNAITKVE GDSLVKQDNL GIITLGKERG GLKVDFANRD GLDRTLSGVK EAVNDNEAVN KGQLDADISK VNNNVTNKFN ELTQNITNVT QQVKGDALLW SDEANAFVAR HEKSKLEKGV SKATQENSKI TYLLDGDISK GSTDAVTGGQ LYSLNEQLAT YFGGDAKYEN GQWTAPTFKV KTVNGEGKEE EQTYHNVAAA FEGVGTSFTN IKSEITKQIN NEISNVKGDS LVKKDLATNL ITIGKEVAGT EINIASVSKA DRTLSGVKEA VKDNEAVNKG QLDTNIKKVE DKLTEAVGKV TQQVKGDALL WSNEDNAFVA DHGKDSAKTK SKITHLLDGN IASGSTDAVT GGQLYSLNEQ LATYFGGGAK YENGQWTAPT FKVKTVNGDG KEEEQTYQNV AEALTGVGTS FTNVQNKITN EITNQVNNAI TKVEGDSLVK QDNLGIITLG KERGGLKVDF ANRDGLDRTL SGVKEAVNDN EAVNKGQLDA NISKVNNNVT NKFNELTQNI TNVTQQVQGD TLLWSDEANA FVARHEKSKL EKGVSKATQE NSKITYLLDG DISKGSTDAV TGGQLYSLNE QLATYFGGGA KYENGEWTAP TFKVKTVNGE GKEEEQTYHN VAAAFEGVGT SFTNIKSEIT KQIDNEIINV KGDSLVKRDL ATNLITIGKE IEGSAINIAN KSGEARTISG VKEAVNNNEA VNKGQLDTNI KKVEDKLTEA VGKVTQQVKG DALLWSNEDN AFVADHGKDS AKTKSKITHL LDGNIASGST DAVTGGQLYS LNEQLATYFG GGAKYENGQW TAPSFKVKTV KEDGKEEEQT YQNVAEALTG VGTSFTNVKN EITKQINHLQ SDDSAVVHYD KNKDETGTIN YASVTLGKGK DSAAVTLHNV ADGSISKDSR DAINGGQIHT IGEDVAKFLG GDAAFKDGAF TGPTYKLSNI DAKGDVQQSE FKDIGSAFAG LDTNIKNVNN NVTNKFNELT QSITNVTQQV KGDSLLWSDE ANAFVARHEK SKLEKGASKA IQENSKITYL LDGNVSKGST DAVTGGQLYS MSNMLATYLG GNAKYENGEW TAPTFKVKTV NGEGKEEEQT YQNVAEALTG VGTSFTNIKS EIAKQINHLQ SDDSAVIHYD KNKDETGTIN YASVTLGKGE DSAAVALHNV AAGNIAKDSR DAINGSQLYS LNEQLLTYFG GNAGYKDGQW IAPKFQVSQF KSDGSSGEKE SYDNVAAAFE GVNKSLAGMN ERINNVVTAG QNVSSNSLNW NETEGGYDAR HNGVDSKLTH VENGDVSEKS KEAVNGSQLW NTNEKVEAVE KDVKNIEKKV QDIATVADSA VKYEKDSTGK KTNVIKLVGG SESDPVLIDN VADGDIKEGS KQAVNGGQLR DYTEKQMKIV LEDAKKYTDE RFNDVVNNGV NEAKAYTDMK FEALSYAVED VRKEARQAQL LVWRYLTYVT MIYRDL
NT Sequence
atgaaaaaat tatctgtcac atcaaagaga caatataatt tatatgcttc gcctatttct cgacgtttat ctttgttaat gaagctctca ttggaaactg taacagttat gttcttattg ggtgcatctc ctgtattggc ttcgaatctt gcgcttacag gagcaaagaa tctgagtcaa aactctccag gtgtaaatta ctctaaaggt agccatggta gtattgttct ctctggtgat gatgattttt gcggtgcgga ttatgttctt ggtcgtggag gcaattctac tgtacgtaat gggattccaa taagtgtaga agaagaatat gagagatttg tcaaacaaaa attaatgaat aatgctactt ctccttatag tcagagttca gagcaacaag tttggactgg tgatgggcta acaagcaaag gttcgggtta tatgggaggg aagtcgactg acggtgataa aaatatcttg cctgaggctt atggtatata ttcttttgca actggttgtg gttcttctgc gcaggggaat tattcagttg catttggtgc aaatgcaact gcacttactg gggggtcgca agcttttggt gttgctgcac ttgcaagtgg aagggtaagt gttgctattg gtgtagggtc agaagcgacg ggagaggctg gagtttcttt gggtggactc tcaaaggcag ctggtgctcg tagtgttgct atagggacgc gggccaacgc ttacggtgaa gaatctattg cgataggtgg tggcttaaaa cagggcagtg ataataagat cggttcagct gtagcgcagg gtctgaaagc gatttctata ggttctgatt ctgttggttt tcagcactat gcagttgcta ttggtgctaa atcccgtgct cttctcttga aaagtgttgc cttgggttct tattctgttg ctgatgttga tgctggcgtt agaggttatg atcctgtgga ggatgagcca tcgaaaaacg ttagttttgt atggaaaagc tctgtaggtg ctgttagtgt tggtaatcgt aaagaaggct taacgcgaca aattatagga gttgcagctg gtactgaaga cactgatgca gtaaatgttg cacagctaaa agcattaagg ggaatgatat cagaaaaagg aggttggaat cttactgtta ataatgacaa taatacagtt gttagttcag gtggtgcatt agatttgtca tctggaagta aaaatctcaa aattgcaaaa gatggaaaaa agaataatgt aacctttgat gtcgctaggg atctcacgtt aaagagcata aaattagacg gtgttacttt aaatgaaaca ggtttattta ttgcaaacgg accacaaatc accgcttcag gtattaatgc tggtagtcaa aaaattacag gcgtagcaga gggtactgat gcgaacgatg cagtaaactt tggacaactg aagaaaattg aaacagaagt caaagaacaa gtagcagcca gtggctttgt gaaacaggat agcgatacaa aataccttac cattggtaag gatacagatg gcgatacaat caatattgct aataacaaaa gtgataagcg tactctcatg ggcataaagg agggtgacat ctcaaaagac tcgagtgaag ctatcaccgg ttcacagcta tttactacaa atcaaaatgt gaaaactgta tcagataatc tccaaacagc agccacgaat atagctaaga cttttggagg tgatgccaaa tatgaagatg gtgaatggac tgctccgaca tttaaagtta aaactgttac cggtgaaggc aaggaagaag agaagacgta tcagaatgta gcggatgcct tggctggagt tggaagttcc atcaccaatg ttcagaataa agtgactgaa caagttaata atgcgattac taaagtggaa ggcgatgcct tattatggag cgatgaagct aatgcctttg tggcgcgtca tgaaaagagt aagttagaaa aaggcgcatc taaagcgaca caagaaaaca gcaagattac gtatctgtta gatggtgatg tttcgaaaga ttccacggat gctattacag gtaaacagct ttattcatta ggtgataaga ttgcatctta tttaggtggt aacgctaaat atgagaatgg tgaatggact gcacctacct ttaaggttaa aacagttaag gaagatggta aggaagaaga gcagacgtat cataatgtag cagcggcttt tgaaggagtt ggtacgtctt tcacgaatgt gaaaaatgaa attactaaac agattaatca tctccagtct gatgattcag cggttgttca ttatgataaa gatgataaaa acggcagtat taattatgcg agtgtaacct tgggtaaagg taaagattct gcagctgtta cccttcataa tgtcgctgca ggtaatattg ctaaggattc acatgatgca atcaatggta gccaaattta ttctctgaac gagcaacttg cgacctattt tggtggcggt gctggttata ataaggaagg caaatggact gctccaactt ttacagtcaa gacagttaag gaagatggtg aggaagaaga gaagacgtat cagaatgtag cggaagcttt gactggagtt ggtacgtctt tcaccaatat aaaaagtgag attactaaac agattgctaa tgagattagc aatgtaacag gtgatagtct tgttaagaaa gatctcgata cgaatcttat caccattggt aaagaagtag caggtactga aatcaatata gcaagcgttt ctaaagctga ccggactctt tctggtgtta aggaagcagt aaaagataat gaagctgtta acaaagggca gcttgataaa ggtttgaagc atctttctga cagtctccag tccgaagatt cagcagttgt tcattatgat aaaaagacag atgaaactgg tggcattaat tatacgagcg tgacgttggg aggtaaagat aagacccctg ttgcccttca taatgtcgct gatggtagta tttccaagga ttcacatgat gccatcaatg gcggacagat tcatacaatc ggtgaggatg ttgcaaaatt cttgggtgga gcagcaagct ttaacaacgg tgcttttacc ggcccaactt ataagttgtc gaatattgat gcaaagggtg atgtacaaca gagtgagttt aaagatatag gttcagcctt tgcgggtctt gatacgaaca tcaagaatgt caataataat gtaacgaata agtttaatga acttactcaa aacataacga atgttacgca acaggtaaaa ggcgatgcct tattatggag cgatgaagcc aatgcctttg tggcgcgtca tgaaaagagc aagttaggaa aaggtgcatc taaagcgaca caagaaaaca gcaagattac gtatctgtta gatggtgatg tttcgaaaga ttccacggat gctattacag gtaaacagct ttattcatta ggtgataaga ttgcatctta tttaggtggt aacgctaaat atgaggatgg tgaatggact gcacctacct ttaaggttaa aacagttaag gaagatggta aggaagaaga gaagacttat cagaatgtag cggaagcttt gactggagtt ggtacgtctt tcacgaatgt gaaaaatgaa attactaaac agattaatca tctccagtct gatgattcag cggttgttca ttatgataag aataaagatg aaactggtgg cattaattat gcgagtgtaa ccttgggtaa aggtaaagat tctgcagctg ttacccttca taatgttgct gatggtagta tttccaagga ttcacgtgat gccatcaatg gtagccaaat ttattctctg aacgagcaac ttgcgaccta ttttggcggc ggtgctaagt acgagaatgg ccaatggacc gctcctattt ttaaagtcaa gacagttaag gaagatggtg aggaagaaga gaagacgtat cagaatgtag cggaagcttt gactggagtt ggtacgtctt tcacaaatat aaagagtgag attactaaac agattgctaa tgagattagc agtgtaacag gtgatagtct tgttaagaaa gatctcgcca cgaatcttat caccattggt aaagaagtag caggtactga aatcaatata gcaagcgttt ctaaagctga ccggactctt tctggtgtta aggaagcagt aaaagataat gaagctgtta acaaagggca gcttgatacc aatatcaaga aagtagaaga taaattaaca gaagcagtcg gtaaagttac gcaacaggtg aaaggtgatg ctttattgtg gagcaatgaa gataacgcgt ttgttgctga tcatggtaag gatagtgcaa agacaaagag caagattaca catttattag atggaaatat tgcgtctggc tcaaccgatg ccgttaccgg tggtcaactc tattctctga acgagcaact tgcgacctat tttggcggcg gtgctaagta cgagaatggc caatggactg cacctacctt taaggttaaa acagttaacg gtgaaggcaa ggaagaagag cagacttatc agaatgtagc ggaagctttg actggagttg gtgcgtcttt catgaatgtt cagaataaaa ttactaatga aattaccaat caagttaata acgcaattac gaaagtagaa ggcgatagtc ttgtgaagca agataatctt ggtattatta cgcttggtaa agaaagaggt ggtttgaaag ttgattttgc aaatcgtgat ggtttagatc ggactctttc tggtgtaaag gaagcggtaa acgataatga agcagttaat aaaggccagc ttgatgccga tatcagtaaa gttaataata atgtaacgaa taagtttaat gaacttactc aaaacataac gaatgttacg caacaggtaa aaggcgatgc cttattatgg agcgatgaag ctaatgcctt tgtggcgcgt catgaaaaga gtaagttaga aaaaggcgta tctaaagcga cacaagaaaa tagcaagatt acgtatctgt tagatggtga tatttcgaaa ggttccacgg atgccgttac cggtggtcag ctttattctc tgaacgagca acttgcgacc tattttggtg gcgatgctaa gtacgagaat ggccaatgga ctgcacctac ctttaaggtt aaaacagtta acggtgaagg caaggaagaa gagcagacgt atcataatgt agcagcagct tttgaaggag ttggtacgtc tttcaccaat ataaaaagtg agattactaa acagattaat aatgagatta gcaatgtaaa aggtgatagt cttgttaaga aagatctcgc cacgaatctt atcaccattg gtaaagaagt agcgggtact gaaatcaata tagcaagcgt ttctaaagct gaccggactc tttctggtgt aaaggaagca gtaaaagata atgaagctgt taacaaaggg cagcttgata ccaatatcaa gaaagtagaa gataaattaa cagaagcagt cggtaaagtt acgcaacagg taaaaggtga tgctttattg tggagcaatg aagataacgc gtttgttgct gatcatggta aggatagcgc aaagacaaag agcaagatta cacatttatt agatggaaat attgcgtctg gctcaaccga tgccgttacc ggtggtcagc tttattctct gaacgagcaa cttgcgacct attttggcgg cggtgctaag tacgagaatg gccaatggac tgcacctacc tttaaggtta aaacagttaa cggagatggc aaggaagaag agcagactta tcagaatgta gcggaagctt tgactggagt tggtacgtct ttcacgaatg ttcagaataa aattactaat gaaattacca atcaagttaa taacgcaatt acgaaagtag aaggcgatag tcttgtgaag caagataatc ttggtattat tacgcttggt aaagaaagag gtggtttgaa agttgatttt gcaaatcgtg atggtttaga tcggactctt tctggtgtaa aggaagcggt aaacgataat gaagcagtta ataaaggcca gcttgatgcc aatatcagta aagttaataa taatgtaacg aataagttta atgaacttac tcaaaacata acgaatgtta cacaacaagt tcaaggtgat actttattat ggagcgatga agctaatgcc tttgtggcgc gtcatgaaaa gagtaagtta gaaaaaggcg tatctaaagc gacacaagaa aatagcaaga ttacgtatct gttagatggt gatatttcga aaggttccac ggatgccgtt accggtggtc agctttattc tctgaacgag caacttgcga cctattttgg cggcggtgct aagtacgaga atggtgaatg gaccgcacct acctttaagg ttaaaacagt taacggtgaa ggcaaggaag aagagcagac gtatcataat gtagcagcag cttttgaagg agttggtacg tctttcacca atataaaaag tgagattact aaacagattg ataatgagat tatcaatgta aaaggtgata gtcttgttaa gagagatctc gctacgaatc tcatcaccat tggtaaagaa atagaaggca gtgcaatcaa tattgctaat aagagtggtg aagctcggac catttctggt gtaaaggaag cagtaaacaa taatgaagct gttaacaaag ggcaacttga taccaatatc aagaaagtag aagataaatt aacagaagca gtcggtaaag ttacgcaaca ggtaaaaggt gatgctttat tgtggagcaa tgaagataac gcgtttgttg ctgatcatgg taaggatagc gcaaagacaa agagtaagat tacacattta ttagatggaa atattgcgtc tggctcaacc gatgccgtta ccggtggtca actctattct cttaacgagc aacttgcgac ctattttggc ggcggtgcta agtacgagaa tggccaatgg actgcaccta gctttaaggt taaaacagtt aaggaagatg gcaaggaaga agagcagacg tatcagaatg tagcggaagc tttgactgga gttggtacgt ctttcacgaa tgtgaaaaat gaaattacta aacagattaa tcatctccag tctgatgatt cagcggttgt tcattatgat aagaataaag atgaaactgg taccattaat tatgcgagtg taaccttggg taaaggtaaa gattctgcag ctgttaccct tcataatgtc gccgatggta gtatttccaa ggattcacgt gatgccatca atggtggaca gattcataca atcggtgagg atgttgcaaa attcttgggt ggagatgcag cttttaaaga tggtgctttt accggcccaa cttataagtt gtcgaatatt gatgcaaagg gtgatgtaca acagagtgag tttaaagata taggttcagc ctttgcgggt cttgatacga acatcaagaa tgtcaataat aatgtaacga ataagtttaa tgaacttact caaagcataa cgaatgttac gcaacaggta aaaggcgatt ccttattatg gagcgatgaa gccaatgcct ttgtggcgcg tcatgaaaag agcaagttag aaaaaggtgc atctaaagcg atacaagaaa atagcaagat tacgtatctg ttagatggta atgtttcgaa aggttccacg gatgccgtta ctggtggtca gctttattca atgagcaaca tgcttgcgac ctatttaggt ggtaacgcta aatatgagaa tggtgaatgg accgcaccta cctttaaggt taaaacagtt aacggtgaag gcaaggaaga agagcaaact tatcagaatg tagcggaagc tttgactgga gttggtacgt ctttcaccaa tataaaaagt gagattgcca aacagattaa tcatctccag tctgatgatt cagcggttat tcattatgat aagaataaag atgaaactgg caccattaat tatgcgagtg taactttggg taaaggtgaa gattctgcag ctgttgccct tcataatgtc gctgcaggta atattgctaa ggattcacgt gatgcaatca atggttctca gctttattct ttgaacgagc agttattgac ctattttggc ggtaatgctg gctataaaga tgggcaatgg atagctccca aattccaagt ttcgcagttc aagagtgatg gtagttctgg tgagaaggag agctatgata atgtagcggc tgcgtttgaa ggagttaaca aaagtcttgc aggtatgaac gagcgtatta ataatgttgt tactgctggc cagaatgttt cgtcgaacag tttaaattgg aatgagacag agggaggtta tgacgctcgt cataatggtg tggacagtaa gctcacgcat gtagagaatg gtgacgtatc cgaaaaatcg aaagaagccg ttaatggaag tcaactatgg aatacgaatg agaaagttga agcggttgag aaggatgtaa agaatattga gaagaaggta caagatattg ctacagtagc agatagtgct gttaagtatg agaaagatag tactggcaag aaaacgaatg taatcaaatt agttggtggg agtgaaagtg atccagtatt gatagacaat gtagcggatg gtgacattaa agaaggctct aagcaggcag tcaatggagg tcagttgcgt gattatactg agaaacagat gaagatagtg cttgaagatg cgaagaaata tacggatgaa cgcttcaatg atgtcgtcaa taatggtgtt aatgaggcta aagcttatac agatatgaag tttgaggctt taagttacgc tgttgaggat gtccgtaaag aagcaagaca ggcgcagcta ttggtttggc ggtatctaac ttacgttact atgatatacc gggatcttta a
Details for BaheA.17833.a.A2.GE33143
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 23kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 90
PERCENT COVERAGE: 29
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAYGIYS FATGCGSSAQ GNYSVAFGAN ATALTGGRKL LVLLHLQVEG
Validated NT Sequence
ttttntttnn ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctg aggcttatgg tatatattct tttgcaactg gttgtggttc ttctgcgcag gggaattatt cagttgcatt tggtgcaaat gcaactgcac ttactggggg tcgcaagctt ttggtgttgc tgcacttgca agtggaaggg taagtgttgc tattggtgta gggtcagaag cgacgggaga ggctggagtt tctttgggtg gactctcaaa ggcagctggt gctcgtagtg ttgctatagg gacgcgggcc aacgcttacg gtgaagaatc tattgcgata gggggtggct taaaacaggg cagtgataat aagatcggtt cagctgtagc gcagggtctg aaagcgattt ctataggttc tgattctgtt ggttttcagc actatgcagt tgctattggt gctaaatccc gtgctcttct cttgaaaagt gttgccttgg gttcttattc tgttgctgat gttgatgctg gcgttagagg ttatgatcct gtggaggatg agccatcgaa aaacgttagt tttgtatgga aaagctctgt aggtgctgtt agtgttggta atcgtaaaga angcttaacg cgacaaatta taggagttgc agctggtact gaagacactg atgcagtaaa tgttgcacag ctataataaa cagcacgaac aagttctgca gccnagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAYGIYS FATGCGSSAQ GNYSVAFGAN ATALTGGSQA FGVAALASGR VSVAIGVGSE ATGEAGVSLG GLSKAAGARS VAIGTRANAY GEESIAIGGG LKQGSDNKIG SAVAQGLKAI SIGSDSVGFQ HYAVAIGAKS RALLLKSVAL GSYSVADVDA GVRGYDPVED EPSKNVSFVW KSSVGAVSVG NRKEGLTRQI IGVAAGTEDT DAVNVAQL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctgaggct tatggtatat attcttttgc aactggttgt ggttcttctg cgcaggggaa ttattcagtt gcatttggtg caaatgcaac tgcacttact ggggggtcgc aagcttttgg tgttgctgca cttgcaagtg gaagggtaag tgttgctatt ggtgtagggt cagaagcgac gggagaggct ggagtttctt tgggtggact ctcaaaggca gctggtgctc gtagtgttgc tatagggacg cgggccaacg cttacggtga agaatctatt gcgataggtg gtggcttaaa acagggcagt gataataaga tcggttcagc tgtagcgcag ggtctgaaag cgatttctat aggttctgat tctgttggtt ttcagcacta tgcagttgct attggtgcta aatcccgtgc tcttctcttg aaaagtgttg ccttgggttc ttattctgtt gctgatgttg atgctggcgt tagaggttat gatcctgtgg aggatgagcc atcgaaaaac gttagttttg tatggaaaag ctctgtaggt gctgttagtg ttggtaatcg taaagaaggc ttaacgcgac aaattatagg agttgcagct ggtactgaag acactgatgc agtaaatgtt gcacagctat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BaheA.17833.a.A4.GE33144
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 15kDa
OBSERVED MW: 15kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SXXGXXKQDS DTKYLTIGKD TDGDTINIAN NKSDKRTLMG IKEGDISKDS SEAITGSQLF TTNQNVKTVS DNLQTAATNI AKTFGGDAKY EDGEWTAPTF KVKTVTGEGK EEEKTYQNVA DALAGV
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtccnggt tcganganng gnttngngaa acaggatagc gatacaaaat accttaccat tggtaaggat acagatggcg atacaatcaa tattgctaat aacaaaagtg ataagcgtac tctcatgggc ataaaggagg gtgacatctc aaaagactcg agtgaagcta tcaccggttc acagctattt actacaaatc aaaatgtgaa aactgtatca gataatctcc aaacagcagc cacgaatata gctaagactt ttggaggtga tgccaaatat gaagatggtg aatggactgc tccgacattt aaagttaaaa ctgttaccgg tgaaggcaag gaagaagaga agacgtatca gaatgtagcg gatgccttgg ctggagttta ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccan nnnnnnnnnn gcnnncnnnn nnncnacnnn nnnncngcnn nnnnncnaan nnnnncnnnn gncnnnnnnn nnnangnnnn nnnnnnnnnn nnnncnnnnn aannnnngnn ngnngncnnn ncnnnnnnnn accnncnncn cgngaaanan nnnaccctnn cagccncnnn ccgnnnnnnn nnnnnannnc cnnannngnn nctcncnncn nccnccnnnn nnnnnannnn ncnnnnccan aannnanncn gnnnnncnnn nnncncnaan nnannnnnna annnnnnnnn nannncnnnn nntaatnnnn nnnnnnnnnn nnnnnnnnnn ntncnnnnnn nccnnngngg tcnnnnnann nngnnnnnnn nccccctnnn nccntnntnn nnnnngnnan nnnnngnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGFVKQDS DTKYLTIGKD TDGDTINIAN NKSDKRTLMG IKEGDISKDS SEAITGSQLF TTNQNVKTVS DNLQTAATNI AKTFGGDAKY EDGEWTAPTF KVKTVTGEGK EEEKTYQNVA DALAGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtggcttt gtgaaacagg atagcgatac aaaatacctt accattggta aggatacaga tggcgataca atcaatattg ctaataacaa aagtgataag cgtactctca tgggcataaa ggagggtgac atctcaaaag actcgagtga agctatcacc ggttcacagc tatttactac aaatcaaaat gtgaaaactg tatcagataa tctccaaaca gcagccacga atatagctaa gacttttgga ggtgatgcca aatatgaaga tggtgaatgg actgctccga catttaaagt taaaactgtt accggtgaag gcaaggaaga agagaagacg tatcagaatg tagcggatgc cttggctgga gtttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa