SSGCID: Target Page

BaheA.17383.a

Type IV secretion system protein virB9 (VirB type IV secretion machinery)

CENTER ID:	BaheA.17383.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.17383.a.B2.GE34959	mature protein		26	288

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1453
RefSeq:	YP_034058.1
UniProt:	Q6G2B3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMRILKTLFL AFIAAISCYT TPSFAETAPV SARKDNRIRF VNYDPYNVTK IIGSIRSSVQ LEFADDEEVT YVGIGNSVAW QVAPAGHFVF LKPREVQPVT NLQIVTSRQD GTKRSYQFEL QVREGDVSAG NDTYFLVKFR YPEDEALRKK LAEAAKAAQR EENFVNDIFN THEDFGPRNW AYEAQGSPLI EPASVYDNGK TTTFTFLGNT EIPAIYLVSL DGQEALVPKT IKGNKVIVHA TAAQFTLRRG NDVLCIFNKR FVPAGVNPET GTTSPSVQRK VNIGNGYE

NT Sequence

atgatgagga ttttaaaaac actcttttta gctttcatag ctgcaataag ttgctatacg accccctcat ttgctgaaac agcacctgtg agtgcacgca aagacaatcg catcagattt gtcaattatg acccctataa tgtgacaaaa atcattggct ccattcgttc ttctgttcaa cttgaatttg ccgatgatga agaagtaacc tatgtaggga ttggcaattc cgttgcttgg caagttgcac cggctggcca ctttgtattt ctaaaaccac gcgaagttca acctgttacc aatttacaaa ttgttacaag ccgccaagac ggcacaaaac gctcttatca atttgaacta caagtgcgtg aaggggatgt ttcagcgggc aatgatacat attttctggt aaagtttcgt tatccagaag atgaagcctt gcgtaaaaaa ttagccgaag cagcgaaagc ggcacaacgt gaagaaaatt ttgtcaatga tatttttaac acgcatgaag attttggacc acgcaattgg gcttatgaag cgcaaggctc acccctcatt gaacctgctt ctgtctatga taacggaaaa acaacaacct ttacattttt aggcaatact gaaatccctg ccatttatct tgtctcatta gatggacaag aagctctcgt tcccaaaacg attaaaggaa ataaggtcat tgttcatgcc acagctgcac aattcactct gcgccgcggg aatgacgtgc tgtgtatctt caataaaaga tttgtgcctg caggggttaa ccctgaaact ggtacgacat caccatctgt acaacgtaaa gtgaacatag gaaacggtta tgaatga

Details for BaheA.17383.a.B2.GE34959

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME TAPVSARKDN RIRFVNYDPY NVTKIIGSIR SSVQLEFADD EEVTYVGIGN SVAWQVAPAG HFVFLKPREV QPVTNLQIVT SRQDGTKRSY QFELQVREGD VSAGNDTYFL VKFRYPEDEA LRKKLAEAAK AAQREENFVN DIFNTHEDFG PRNWAYEAQG SPLIEPASVY DNGKTTTFTF LGNTEIPAIY LVSLDGQEAL VPKTIKGNKV IVHATAAQFT LRRGNDVLCI FNKRFVPAGV NPETGTTSPS VQRKVNIGNG YE

Validated NT Sequence

atcttactca ctattcataa ccgtttccta tgttcacttt acgttgtaca gatggtgatg tcgtaccagt ttcagggtta acccctgcag gcacaaatct tttattgaag atacacagca cgtcattccc gcggcgcaga gtgaattgtg cagctgtggc atgaacaatg accttatttc ctttaatcgt tttgggaacg agagcttctt gtccatctaa tgagacaaga taaatggcag ggatttcagt attgcctaaa aatgtaaagg ttgttgtttt tccgttatca tagacagaag caggttcaat gaggggtgag ccttgcgctt cataagccca attgcgtggt ccaaaatctt catgcgtgtt aaaaatatca ttgacaaaat tttcttcacg ttgtgccgct ttcgctgctt cggctaattt tttacgcaag gcttcatctt ctggataacg aaactttacc agaaaatatg tatcattgcc cgctgaaaca tccccttcac gcacttgtag ttcaaattga taagagcgtt ttgtgccgtc ttggcggctt gtaacaattt gtaaattggt aacaggttga acttcgcgtg gttttagaaa tacaaagtgg ccagccggtg caacttgcca agcaacggaa ttgccaatcc ctacataggt tacttcttca tcatcggcaa attcaagttg aacagaagaa cgaatggagc caatgatttt tgtcacatta taggggtcat aattgacaaa tctgatgcga ttgtctttgc gtgcactcac aggtgctgtt tccatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa cagcacctgt gagtgcacgc aaagacaatc gcatcagatt tgtcaattat gacccctata atgtgacaaa aatcattggc tccattcgtt cttctgttca acttgaattt gccgatgatg aagaagtaac ctatgtaggg attggcaatt ccgttgcttg gcaagttgca ccggctggcc actttgtatt tctaaaacca cgcgaagttc aacctgttac caatttacaa attgttacaa gccgccaaga cggcacaaaa cgctcttatc aatttgaact acaagtgcgt gaaggggatg tttcagcggg caatgataca tattttctgg taaagtttcg ttatccagaa gatgaagcct tgcgtaaaaa attagccgaa gcagcgaaag cggcacaacg tgaagaaaat tttgtcaatg atatttttaa cacgcatgaa gattttggac cacgcaattg ggcttatgaa gcgcaaggct cacccctcat tgaacctgct tctgtctatg ataacggaaa aacaacaacc tttacatttt taggcaatac tgaaatccct gccatttatc ttgtctcatt agatggacaa gaagctctcg ttcccaaaac gattaaagga aataaggtca ttgttcatgc cacagctgca caattcactc tgcgccgcgg gaatgacgtg ctgtgtatct tcaataaaag atttgtgcct gcaggggtta accctgaaac tggtacgaca tcaccatctg tacaacgtaa agtgaacata ggaaacggtt atgaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat