BaheA.17330.d

BepF protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaheA.17330.d
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.17330.d.B4.GE37029 BID3 650 785
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.17330.d.B3.PS01917 BID2 435 570

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1472
RefSeq: YP_034068.1
UniProt: A0A0H3LZH0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKNQPSSST SPSVKELKKR YEQTAAEAST LHQLSAGSAP KEKKQPSSRL EELRKRYEQT ASLTASQEAL SARGFPKEKR QPHTQQSPEQ LPIHHEQQFA VTPQQSSSQT PLYATPLPQQ PVRTPPQRPP RAKDKELHKT APQDSTPLYA TPSPQQSVQT PPQRPPRAKD KELHKAASQD STPLYATPSP HKQQPMRERA QSNTSSQDSE PLYATPLPQR HQPMRERAQS NTSNQDSEPL YATPLPQRHQ PVRKRTQSNT SSQDSEPLYA TPLPQRRQPV RKRTQSNTSS QDNEPLYATA APSQSPRMEE KSVNTMQRNL LLEAYKEEIK YWCGIVYGDR LILQKRIEEI QENPALGEQL SWEVSEHPKS ISKLAGKKML GVKTKARRKA EENYLALRDT INSYVYTLKY SQQKPLHAPH TEHTRDEQQT HRTQSAEKER APLSNQELAD RIRTEPSVLY SQTEVQYWSK IVFGNPYILQ YRIEDIQKNP DMGEEFSWQV ANNPSLFSPL AGKRILGIKN DARRHAEASL SCLCSAIEGY ADAVKQAKED IVQEYQVQQS RQELSTEPAK QLQKQQTLSK PHKLPEHSPT DAHQETTQTS MQTEWERLGA RPRTVTTHKK HTQESTLPTS HAEQKTTREQ TTENQQKSLT PEKEKTPLSH KEIASRVQND LSVQRAQIEI YNWCNIVYNN PLVLQHTTED IKKIPMLGEE LSWQVANFST IFAPLAGKQV LGIKNNARKH AEEAIPSLCT AIDHYTEIVK QVREDIVKNH QEQSPKKDKN VKRQQSLSKP PKLPERSTEI PRHQALEASR QAQQKPSNVR SSEAEKTQKM VLAL
NT Sequence
atgaaaaaaa accaaccatc ctcttccaca tctccctcag tgaaagaatt aaaaaagcgc tatgaacaaa ctgccgcaga agcctctact ctacaccaac tctccgcagg gagtgctcca aaagaaaaaa aacagccttc ctcgaggttg gaagaattaa gaaaacgcta tgaacaaact gcttccctaa cagcttctca agaagctctc tctgcaaggg gttttccaaa agaaaaaaga caacctcaca cacagcaaag tcctgaacaa ttgccaatac accatgaaca gcaatttgct gtaacgccgc aacaaagttc atcacaaacg cctctctacg caacacctct tccacaacaa ccagttcgca cgcctccaca aagaccaccg cgcgcaaaag acaaagagct acacaaaaca gccccccaag acagcacacc cctatacgcg acaccttctc cacaacaatc agttcagaca cctccacaaa gaccaccgcg cgcaaaagac aaggagctac acaaagcagc ctcccaagac agcacacccc tatacgcgac accttctccc cacaaacaac agcccatgag agaaagggcg caaagcaaca catcaagcca agacagcgaa cctctctacg caacacctct tccacaaaga caccagccca tgagagaaag ggcgcaaagc aacacatcaa accaagacag cgaacctctc tacgcaacac ctcttccaca aagacaccag cccgtgagaa aaaggacgca aagcaacaca tcaagccaag acagcgaacc tctctacgca acacctcttc cacaaagacg ccagcccgtg agaaaaagga cgcaaagcaa cacatcaagc caagacaacg aacctctcta cgcaacagct gctccatcac aatcaccacg catggaagag aaatctgtaa acacaatgca acgaaactta ttgcttgaag catataagga agaaattaaa tattggtgcg gaattgttta tggcgatcgg cttattttgc agaaaagaat agaagagatt caagaaaacc ctgctctggg agaacagctg tcatgggagg tttcagaaca tcctaagtct atttctaagc ttgcgggtaa aaaaatgctt ggtgtgaaaa cgaaggcacg cagaaaagct gaagaaaatt atctagcttt gcgtgacacc ataaatagtt atgtttatac tttaaaatat tcacaacaaa aacctttgca tgctccgcat acagaacaca cccgcgatga acaacaaaca cacagaacgc aaagcgctga aaaggagaga gcacctctct ctaatcagga acttgccgac agaattcgaa cagagccatc ggttctatat agccaaacag aagtccaata ttggagcaaa attgtttttg gcaaccctta tattttgcag tacagaattg aagacattca aaaaaatccc gatatgggag aagaattttc atggcaagtt gcaaacaatc ctagtctttt ttctccgctc gcaggaaaga ggatacttgg cataaaaaat gatgcgcgca gacacgcaga agcaagtctt tcttgccttt gtagcgccat tgaaggttac gcagatgctg taaaacaagc aaaagaggat attgtacaag aatatcaagt acaacaaagt cgccaagaac tttctactga accggctaaa cagttgcaaa aacaacaaac tttatccaaa ccacataaac tgcctgaaca ctcaccaaca gacgcgcatc aagaaactac tcaaacctcc atgcaaactg agtgggagcg gcttggtgct cgaccacgca cagtgacaac acacaaaaaa cacacacaag agagcacttt gcctacttct catgcagagc aaaaaacaac gagagagcaa acaactgaaa atcagcaaaa atcccttaca cctgaaaaag aaaaaacacc tctctcacat aaagaaattg caagcagagt tcaaaacgat ctctctgttc aacgtgctca aatagaaatc tacaattggt gtaatattgt ttataataac ccacttgttt tgcaacatac cactgaagat atcaaaaaaa ttcccatgct aggagaagaa ctctcatggc aagttgcaaa cttttctaca atatttgctc cgcttgctgg caagcaagtg cttggcataa aaaataacgc acgtaaacat gctgaagaag ctattccctc cctatgcact gccattgatc attacacaga gattgtaaaa caagtaagag aggacattgt aaaaaaccat caggaacaat ctccaaagaa agataaaaac gtgaaacgac agcaaagctt atcgaaacct cctaaattac ctgagcgctc aacagaaatc ccacgtcatc aggctttgga agcctctagg caagcacaac aaaaaccgtc gaacgttcga tcaagcgaag ctgagaaaac acaaaaaatg gtgctggcac tctaa
Details for BaheA.17330.d.B4.GE37029
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 17kDa
OBSERVED MW: 16kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT PEKEKTPLSH KEIASRVQND LSVQRAQIEI YNWCNIVYNN PLVLQHTTED IKKIPMLGEE LSWQVANFST IFAPLAGKQV LGIKNNARKH AEEAIPSLCT AIDHYTEIVK QVREDIVKNH QEQSPKKDKN VKRQQ
Validated NT Sequence
atcttactca cttattgctg tcgtttcacg tttttatctt tctttggaga ttgttcctga tggtttttta caatgtcctc tcttacttgt tttacaatct ctgtgtaatg atcaatggca gtgcataggg agggaatagc ttcttcagca tgtttacgtg cgttattttt tatgccaagc acttgcttgc cagcaagcgg agcaaatatt gtagaaaagt ttgcaacttg ccatgagagt tcttctccta gcatgggaat ttttttgata tcttcagtgg tatgttgcaa aacaagtggg ttattataaa caatattaca ccaattgtag atttctattt gagcacgttg aacagagaga tcgttttgaa ctctgcttgc aatttcttta tgtgagagag gtgttttttc tttttcaggt gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHTP EKEKTPLSHK EIASRVQNDL SVQRAQIEIY NWCNIVYNNP LVLQHTTEDI KKIPMLGEEL SWQVANFSTI FAPLAGKQVL GIKNNARKHA EEAIPSLCTA IDHYTEIVKQ VREDIVKNHQ EQSPKKDKNV KRQQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacacctg aaaaagaaaa aacacctctc tcacataaag aaattgcaag cagagttcaa aacgatctct ctgttcaacg tgctcaaata gaaatctaca attggtgtaa tattgtttat aataacccac ttgttttgca acataccact gaagatatca aaaaaattcc catgctagga gaagaactct catggcaagt tgcaaacttt tctacaatat ttgctccgct tgctggcaag caagtgcttg gcataaaaaa taacgcacgt aaacatgctg aagaagctat tccctcccta tgcactgcca ttgatcatta cacagagatt gtaaaacaag taagagagga cattgtaaaa aaccatcagg aacaatctcc aaagaaagat aaaaacgtga aacgacagca atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BaheA.17330.d.B3.PS01917
PURIFICATION DATe: 10/17/2013
CONCENTRATION: 1.57mg/ml
OBSERVED MW: 17kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS AEKERAPLSN QELADRIRTE PSVLYSQTEV QYWSKIVFGN PYILQYRIED IQKNPDMGEE FSWQVANNPS LFSPLAGKRI LGIKNDARRH AEASLSCLCS AIEGYADAVK QAKEDIVQEY QVQQSRQELS TEPAK
Validated NT Sequence
atcttactca cttatttagc cggttcagta gaaagttctt ggcgactttg ttgtacttga tattcttgta caatatcctc ttttgcttgt tttacagcat ctgcgtaacc ttcaatggcg ctacaaaggc aagaaagact tgcttctgcg tgtctgcgcg catcattttt tatgccaagt atcctctttc ctgcgagcgg agaaaaaaga ctaggattgt ttgcaacttg ccatgaaaat tcttctccca tatcgggatt tttttgaatg tcttcaattc tgtactgcaa aatataaggg ttgccaaaaa caattttgct ccaatattgg acttctgttt ggctatatag aaccgatggc tctgttcgaa ttctgtcggc aagttcctga ttagagagag gtgctctctc cttttcagcg ctcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHSA EKERAPLSNQ ELADRIRTEP SVLYSQTEVQ YWSKIVFGNP YILQYRIEDI QKNPDMGEEF SWQVANNPSL FSPLAGKRIL GIKNDARRHA EASLSCLCSA IEGYADAVKQ AKEDIVQEYQ VQQSRQELST EPAK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAGCGCTG AAAAGGAGAG AGCACCTCTC TCTAATCAGG AACTTGCCGA CAGAATTCGA ACAGAGCCAT CGGTTCTATA TAGCCAAACA GAAGTCCAAT ATTGGAGCAA AATTGTTTTT GGCAACCCTT ATATTTTGCA GTACAGAATT GAAGACATTC AAAAAAATCC CGATATGGGA GAAGAATTTT CATGGCAAGT TGCAAACAAT CCTAGTCTTT TTTCTCCGCT CGCAGGAAAG AGGATACTTG GCATAAAAAA TGATGCGCGC AGACACGCAG AAGCAAGTCT TTCTTGCCTT TGTAGCGCCA TTGAAGGTTA CGCAGATGCT GTAAAACAAG CAAAAGAGGA TATTGTACAA GAATATCAAG TACAACAAAG TCGCCAAGAA CTTTCTACTG AACCGGCTAA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT