BaheA.17239.a

4-hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH/LytB) (IspG/H)

CENTER ID: BaheA.17239.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.17239.a.A1.GE34613 Full length( BaheA.17239.a ) 1 343
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.17239.a.A1.PW35223 Full length( BaheA.17239.a ) 1 343

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.482
RefSeq: YP_033279.1
UniProt: Q6G4C5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSILSPLIIR LCNPRGFCAG VDRAIQIVLL ALKKYGAPVY VRHEIVHNRY VVEGLQQRGA IFVEELDEIP EEHRNQPVVF SAHGVPKSVP EQADCYNLFY LDATCPLVSK VHKQAMRHQR HRRHVILIGH AGHPEVIGTM GQLEKGGVTL IETVEDALHY QPDDPDNLGF VTQTTLSVED TAEILDVLQR RFPALEPPAA ESICYATTNR QNAVKAAALG SDLFLIVGAP NSSNSRRLVE VAERSGARQS ILVQRADEID FDHLGALSVV SLSAGASAPE IIVDEIISAF RKRYNVTIEL AETVVENETF LVNRELRDVV LTPQDMAFMN GRSEMLKNKN QDM
NT Sequence
ATGTCTATAC TTTCCCCTTT AATAATACGC CTTTGTAATC CACGTGGATT TTGTGCAGGG GTTGATCGCG CTATCCAAAT TGTTCTTCTT GCATTAAAAA AATATGGTGC TCCGGTGTAT GTTCGTCATG AAATTGTACA CAATCGCTAT GTCGTTGAAG GATTGCAGCA ACGGGGGGCC ATTTTTGTAG AAGAACTTGA TGAGATTCCA GAAGAACATC GCAATCAACC TGTTGTATTT TCTGCGCATG GTGTGCCAAA ATCTGTACCA GAACAGGCTG ATTGTTATAA TTTGTTTTAT CTCGACGCAA CATGTCCGTT GGTTTCTAAG GTTCATAAAC AAGCAATGCG TCATCAACGC CATAGACGTC ATGTTATATT GATTGGTCAT GCTGGTCATC CTGAGGTGAT AGGAACAATG GGACAGCTTG AAAAAGGGGG TGTTACGTTA ATTGAAACGG TGGAAGATGC TTTGCATTAT CAACCAGATG ATCCAGATAA TTTAGGATTT GTAACGCAAA CAACACTTTC TGTTGAAGAT ACAGCAGAGA TTCTTGATGT ATTACAAAGA CGTTTCCCTG CGTTGGAACC ACCAGCTGCC GAATCAATTT GCTATGCTAC GACGAATCGC CAAAATGCTG TTAAGGCAGC AGCTCTGGGA AGCGATTTAT TTTTGATTGT TGGTGCACCG AATTCTTCTA ATTCACGACG TTTGGTAGAG GTTGCTGAGA GATCGGGTGC GCGGCAGTCT ATTTTAGTTC AACGGGCTGA TGAAATTGAT TTTGATCATT TAGGTGCTCT TTCTGTCGTA AGTCTTTCAG CGGGAGCTTC TGCTCCTGAA ATTATTGTTG ATGAAATTAT TTCAGCTTTT CGTAAGCGTT ATAATGTGAC AATAGAATTG GCTGAAACAG TGGTGGAAAA CGAGACATTT TTAGTCAATC GCGAATTACG TGATGTTGTT TTGACTCCTC AAGATATGGC TTTTATGAAT GGTCGATCTG AGATGCTAAA AAACAAAAAT CAGGATATG
Details for BaheA.17239.a.A1.GE34613
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSILSPLII RLCNPRGFCA GVDRAIQIVL LALKKYGAPV YVRHEIVHNR YVVEGLQQRG AIFVEELDEI PEEHRNQPVV FSAHGVPKSV PEQADCYNLF YLDATCPLVS KVHKQAMRHQ RHRRHVILIG HAGHPEVIGT MGQLEKGGVT LIETVEDALH YQPDDPDNLG FVTQTTLSVE DTAEILDVLQ RRFPALEPPA AESICYATTN RQNAVKAAAL GSDLFLIVGA PNSSNSRRLV EVAERSGARQ SILVQRADEI DFDHLGALSV VSLSAGASAP EIIVDEIISA FRKRYNVTIE LAETVVENET FLVNRELRDV VLTPQDMAFM NGRSEMLKNK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcta tactttcccc tttaataata cgcctttgta atccacgtgg attttgtgca ggggttgatc gcgctatcca aattgttctt cttgcattaa aaaaatatgg tgctccggtg tatgttcgtc atgaaattgt acacaatcgc tatgtcgttg aaggattgca gcaacggggg gccatttttg tagaagaact tgatgagatt ccagaagaac atcgcaatca acctgttgta ttttctgcgc atggtgtgcc aaaatctgta ccagaacagg ctgattgtta taatttgttt tatctcgacg caacatgtcc gttggtttct aaggttcata aacaagcaat gcgtcatcaa cgccatagac gtcatgttat attgattggt catgctggtc atcctgaggt gataggaaca atgggacagc ttgaaaaagg gggtgttacg ttaattgaaa cggtggaaga tgctttgcat tatcaaccag atgatccaga taatttagga tttgtaacgc aaacaacact ttctgttgaa gatacagcag agattcttga tgtattacaa agacgtttcc ctgcgttgga accaccagct gccgaatcaa tttgctatgc tacgacgaat cgccaaaatg ctgttaaggc agcagctctg ggaagcgatt tatttttgat tgttggtgca ccgaattctt ctaattcacg acgtttggta gaggttgctg agagatcggg tgcgcggcag tctattttag ttcaacgggc tgatgaaatt gattttgatc atttaggtgc tctttctgtc gtaagtcttt cagcgggagc ttctgctcct gaaattattg ttgatgaaat tatttcagct tttcgtaagc gttataatgt gacaatagaa ttggctgaaa cagtggtgga aaacgagaca tttttagtca atcgcgaatt acgtgatgtt gttttgactc ctcaagatat ggcttttatg aatggtcgat ctgagatgct aaaaaacaaa aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSILSPLII RLCNPRGFCA GVDRAIQIVL LALKKYGAPV YVRHEIVHNR YVVEGLQQRG AIFVEELDEI PEEHRNQPVV FSAHGVPKSV PEQADCYNLF YLDATCPLVS KVHKQAMRHQ RHRRHVILIG HAGHPEVIGT MGQLEKGGVT LIETVEDALH YQPDDPDNLG FVTQTTLSVE DTAEILDVLQ RRFPALEPPA AESICYATTN RQNAVKAAAL GSDLFLIVGA PNSSNSRRLV EVAERSGARQ SILVQRADEI DFDHLGALSV VSLSAGASAP EIIVDEIISA FRKRYNVTIE LAETVVENET FLVNRELRDV VLTPQDMAFM NGRSEMLKNK NQD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctatactt tcccctttaa taatacgcct ttgtaatcca cgtggatttt gtgcaggggt tgatcgcgct atccaaattg ttcttcttgc attaaaaaaa tatggtgctc cggtgtatgt tcgtcatgaa attgtacaca atcgctatgt cgttgaagga ttgcagcaac ggggggccat ttttgtagaa gaacttgatg agattccaga agaacatcgc aatcaacctg ttgtattttc tgcgcatggt gtgccaaaat ctgtaccaga acaggctgat tgttataatt tgttttatct cgacgcaaca tgtccgttgg tttctaaggt tcataaacaa gcaatgcgtc atcaacgcca tagacgtcat gttatattga ttggtcatgc tggtcatcct gaggtgatag gaacaatggg acagcttgaa aaagggggtg ttacgttaat tgaaacggtg gaagatgctt tgcattatca accagatgat ccagataatt taggatttgt aacgcaaaca acactttctg ttgaagatac agcagagatt cttgatgtat tacaaagacg tttccctgcg ttggaaccac cagctgccga atcaatttgc tatgctacga cgaatcgcca aaatgctgtt aaggcagcag ctctgggaag cgatttattt ttgattgttg gtgcaccgaa ttcttctaat tcacgacgtt tggtagaggt tgctgagaga tcgggtgcgc ggcagtctat tttagttcaa cgggctgatg aaattgattt tgatcattta ggtgctcttt ctgtcgtaag tctttcagcg ggagcttctg ctcctgaaat tattgttgat gaaattattt cagcttttcg taagcgttat aatgtgacaa tagaattggc tgaaacagtg gtggaaaacg agacattttt agtcaatcgc gaattacgtg atgttgtttt gactcctcaa gatatggctt ttatgaatgg tcgatctgag atgctaaaaa acaaaaatca ggataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BaheA.17239.a.A1.PW35223
PURIFICATION DATe: 3/9/2012
CONCENTRATION: 27.26mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 18
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSILSPLII RLCNPRGFCA GVDRAIQIVL LALKKYGAPV YVRHEIVHNR YVVEGLQQRG AIFVEELDEI PEEHRNQPVV FSAHGVPKSV PEQADCYNLF YLDATCPLVS KVHKQAMRHQ RHRRHVILIG HAGHPEVIGT MGQLEKGGVT LIETVEDALH YQPDDPDNLG FVTQTTLSVE DTAEILDVLQ RRFPALEPPA AESICYATTN RQNAVKAAAL GSDLFLIVGA PNSSNSRRLV EVAERSGARQ SILVQRADEI DFDHLGALSV VSLSAGASAP EIIVDEIISA FRKRYNVTIE LAETVVENET FLVNRELRDV VLTPQDMAFM NGRSEMLKNK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcta tactttcccc tttaataata cgcctttgta atccacgtgg attttgtgca ggggttgatc gcgctatcca aattgttctt cttgcattaa aaaaatatgg tgctccggtg tatgttcgtc atgaaattgt acacaatcgc tatgtcgttg aaggattgca gcaacggggg gccatttttg tagaagaact tgatgagatt ccagaagaac atcgcaatca acctgttgta ttttctgcgc atggtgtgcc aaaatctgta ccagaacagg ctgattgtta taatttgttt tatctcgacg caacatgtcc gttggtttct aaggttcata aacaagcaat gcgtcatcaa cgccatagac gtcatgttat attgattggt catgctggtc atcctgaggt gataggaaca atgggacagc ttgaaaaagg gggtgttacg ttaattgaaa cggtggaaga tgctttgcat tatcaaccag atgatccaga taatttagga tttgtaacgc aaacaacact ttctgttgaa gatacagcag agattcttga tgtattacaa agacgtttcc ctgcgttgga accaccagct gccgaatcaa tttgctatgc tacgacgaat cgccaaaatg ctgttaaggc agcagctctg ggaagcgatt tatttttgat tgttggtgca ccgaattctt ctaattcacg acgtttggta gaggttgctg agagatcggg tgcgcggcag tctattttag ttcaacgggc tgatgaaatt gattttgatc atttaggtgc tctttctgtc gtaagtcttt cagcgggagc ttctgctcct gaaattattg ttgatgaaat tatttcagct tttcgtaagc gttataatgt gacaatagaa ttggctgaaa cagtggtgga aaacgagaca tttttagtca atcgcgaatt acgtgatgtt gttttgactc ctcaagatat ggcttttatg aatggtcgat ctgagatgct aaaaaacaaa aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSILSPLII RLCNPRGFCA GVDRAIQIVL LALKKYGAPV YVRHEIVHNR YVVEGLQQRG AIFVEELDEI PEEHRNQPVV FSAHGVPKSV PEQADCYNLF YLDATCPLVS KVHKQAMRHQ RHRRHVILIG HAGHPEVIGT MGQLEKGGVT LIETVEDALH YQPDDPDNLG FVTQTTLSVE DTAEILDVLQ RRFPALEPPA AESICYATTN RQNAVKAAAL GSDLFLIVGA PNSSNSRRLV EVAERSGARQ SILVQRADEI DFDHLGALSV VSLSAGASAP EIIVDEIISA FRKRYNVTIE LAETVVENET FLVNRELRDV VLTPQDMAFM NGRSEMLKNK NQD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTATACTT TCCCCTTTAA TAATACGCCT TTGTAATCCA CGTGGATTTT GTGCAGGGGT TGATCGCGCT ATCCAAATTG TTCTTCTTGC ATTAAAAAAA TATGGTGCTC CGGTGTATGT TCGTCATGAA ATTGTACACA ATCGCTATGT CGTTGAAGGA TTGCAGCAAC GGGGGGCCAT TTTTGTAGAA GAACTTGATG AGATTCCAGA AGAACATCGC AATCAACCTG TTGTATTTTC TGCGCATGGT GTGCCAAAAT CTGTACCAGA ACAGGCTGAT TGTTATAATT TGTTTTATCT CGACGCAACA TGTCCGTTGG TTTCTAAGGT TCATAAACAA GCAATGCGTC ATCAACGCCA TAGACGTCAT GTTATATTGA TTGGTCATGC TGGTCATCCT GAGGTGATAG GAACAATGGG ACAGCTTGAA AAAGGGGGTG TTACGTTAAT TGAAACGGTG GAAGATGCTT TGCATTATCA ACCAGATGAT CCAGATAATT TAGGATTTGT AACGCAAACA ACACTTTCTG TTGAAGATAC AGCAGAGATT CTTGATGTAT TACAAAGACG TTTCCCTGCG TTGGAACCAC CAGCTGCCGA ATCAATTTGC TATGCTACGA CGAATCGCCA AAATGCTGTT AAGGCAGCAG CTCTGGGAAG CGATTTATTT TTGATTGTTG GTGCACCGAA TTCTTCTAAT TCACGACGTT TGGTAGAGGT TGCTGAGAGA TCGGGTGCGC GGCAGTCTAT TTTAGTTCAA CGGGCTGATG AAATTGATTT TGATCATTTA GGTGCTCTTT CTGTCGTAAG TCTTTCAGCG GGAGCTTCTG CTCCTGAAAT TATTGTTGAT GAAATTATTT CAGCTTTTCG TAAGCGTTAT AATGTGACAA TAGAATTGGC TGAAACAGTG GTGGAAAACG AGACATTTTT AGTCAATCGC GAATTACGTG ATGTTGTTTT GACTCCTCAA GATATGGCTT TTATGAATGG TCGATCTGAG ATGCTAAAAA ACAAAAATCA GGATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA