BaheA.10647.a

Penicillin-binding protein

CENTER ID: BaheA.10647.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.10647.a.A2.GE27646 Mature protein( BaheA.10647.a ) 50 580
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.1180
RefSeq: YP_033902.1
UniProt: A0A0H3M664

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSYFLFSQK RKRLKKQFNI DNFPVRRSYS SRRRLFFSSF CFLILYGVMG ACLISYGFEG GQIEEAKGPR VLQLTARPDI LDRNGRLLAT DIKTYSLFAE PRRIIDVDET IELLSTVLPD LNWQETYKRL KKKSSFSWIQ RGLTPLQKAQ IMALGIPGIG FRTEIRRFYP SGPVLSHILG MVNVDNQGIA GMEKYIDDAG LSDLRAAGLA TEDSLKPVQL SIDVRVQTIV HDELMKAMKR YKAIAAGAVI LNIHTSEVLA LASLPDFDPG NPVDALKSDR LNRMTAGTFE MGSIIKSFTT AMALDSDIFH LNSIIDASKP LQASRGFTIH DFHGKNRPLT LWEVFIYSSN IGSAKEAMAI GIDGHRAFLK RLGLLDRMTT ELPEVAHPLV PNHWKDIHSM TISFGHGMAT TPLQTAVAAA ALMNGGWLIA PTFLKRTRDQ ALQHAKQVLQ AKTSQNMRYL YKLNSDIGSG RNAKVEGYRV GGKTGTAEKV ENGKYSKTKN FNSFLAAFPI EDPDYVVLTI IDEPQPEDGK YSATAAMNAG PILANIIRRS ANFLGIKPDF QKEYEPILTT ENSLSFVKKR
NT Sequence
ATGAAATCAT ACTTTCTCTT TTCGCAGAAA AGAAAGCGCT TAAAAAAACA GTTCAATATT GATAATTTTC CTGTTCGTCG CTCATATTCT TCTCGTCGAC GCTTATTTTT TTCTTCTTTC TGTTTTCTCA TTTTATATGG TGTTATGGGG GCTTGTCTTA TTTCTTATGG GTTTGAAGGG GGACAGATTG AAGAAGCAAA AGGACCACGA GTCCTTCAAT TGACTGCACG GCCTGATATT CTTGATCGTA ATGGTCGTTT ATTGGCAACA GATATTAAGA CCTATTCGCT TTTTGCTGAA CCACGACGTA TTATTGATGT CGATGAAACA ATAGAATTGC TCTCAACAGT TTTGCCCGAT CTCAATTGGC AGGAAACTTA TAAACGTCTT AAAAAAAAGT CCAGTTTTTC TTGGATTCAG CGTGGGTTAA CGCCACTACA GAAAGCGCAA ATTATGGCTC TTGGTATTCC AGGAATTGGT TTTCGTACCG AAATTCGTCG TTTTTATCCC AGTGGACCTG TACTTTCACA TATTCTCGGC ATGGTTAATG TTGATAATCA GGGAATAGCG GGTATGGAAA AATATATCGA TGATGCTGGC TTGAGTGATT TGCGTGCTGC TGGTCTTGCA ACGGAAGATT CTTTAAAGCC AGTTCAACTT TCGATTGATG TGCGTGTTCA GACAATTGTC CATGATGAAC TTATGAAGGC AATGAAGCGT TATAAGGCGA TTGCTGCAGG AGCTGTTATT TTAAATATCC ACACAAGTGA AGTTTTGGCT TTGGCATCAC TACCAGATTT TGATCCTGGA AATCCTGTTG ATGCTTTGAA AAGTGATCGT CTCAATCGGA TGACAGCTGG AACTTTTGAA ATGGGATCTA TCATTAAAAG TTTTACGACC GCGATGGCCC TTGATTCAGA TATATTCCAT TTAAACAGTA TCATTGATGC TTCAAAACCT CTGCAAGCAA GTAGAGGTTT TACTATTCAT GATTTTCATG GGAAAAATCG TCCCTTAACG TTATGGGAGG TTTTTATTTA TTCTTCTAAC ATTGGTTCTG CTAAGGAGGC AATGGCGATA GGGATTGATG GACATCGTGC TTTTTTGAAA CGACTTGGTT TACTTGATCG AATGACAACA GAATTGCCTG AAGTTGCCCA TCCTCTTGTG CCAAATCATT GGAAGGACAT CCATTCTATG ACAATTTCTT TTGGACATGG TATGGCAACA ACGCCCTTGC AAACAGCAGT AGCTGCTGCC GCTTTAATGA ATGGTGGTTG GTTAATTGCT CCTACGTTTT TAAAACGCAC AAGAGATCAA GCTTTGCAAC ATGCAAAACA AGTTTTGCAG GCTAAAACGA GTCAAAATAT GCGTTATCTT TATAAATTAA ATAGTGATAT TGGTTCTGGG CGTAATGCAA AAGTAGAAGG TTATCGCGTT GGTGGTAAGA CAGGAACAGC CGAAAAAGTT GAAAATGGAA AATATTCTAA AACAAAAAAT TTCAATAGTT TTCTCGCTGC TTTTCCCATT GAAGATCCTG ATTATGTTGT TTTAACAATT ATCGATGAAC CTCAGCCTGA AGATGGAAAG TATTCAGCAA CAGCAGCAAT GAATGCAGGG CCGATACTTG CTAATATTAT TCGCCGTTCA GCGAATTTTC TTGGAATAAA ACCAGATTTT CAGAAAGAAT ATGAGCCTAT TTTAACCACA GAGAACAGTT TAAGTTTTGT TAAAAAACGG
Details for BaheA.10647.a.A2.GE27646
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 60kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGACLISYG FEGGQIEEAK GPRVLQLTAR PDILDRNGRL LATDIKTYSL FAEPRRIIDV DETIELLSTV LPDLNWQETY KRLKKKSSFS WIQRGLTPLQ KAQIMALGIP GIGFRTEIRR FYPSGPVLSH ILGMVNVDNQ GIAGMEKYID DAGLSDLRAA GLATEDSLKP VQLSIDVRVQ TIVHDELMKA MKRYKAIAAG AVILNIHTSE VLALASLPDF DPGNPXDALK SDRLXRMTAG TFEMGSIIKS FTTAMALDSD IFHLNSIIDA SKPLQASRGF TIHDFHGKNR PLTLWEVFIY XSNIGSAKEA MAIGIDGHRA FLKRLGLLDR MTTELPEVAH PLVPNHWKDI HSMTISFGHG MATTPLQTAV AAAALMNGGW LIAPTFLKRT RDQALQHAKQ VLQAKTSQNM RYLYKLNSDI GSGRNAKVEG YRVGGKTGTA EKVENGKYSK TKNFNSFLAA FPIEDPDYVV LTIIDEPQPE DGKYSATAAM NAGPILANII RRSANFLGIK PDFQKEYEPI LTTENSLSLL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggggg cttgtcttat ttcttatggg tttgaagggg gacagattga agaagcaaaa ggaccacgag tccttcaatt gactgcacgg cctgatattc ttgatcgtaa tggtcgttta ttggcaacag atattaagac ctattcgctt tttgctgaac cacgacgtat tattgatgtc gatgaaacaa tagaattgct ctcaacagtt ttgcccgatc tcaattggca ggaaacttat aaacgtctta aaaaaaagtc cagtttttct tggattcagc gtgggttaac gccactacag aaagcgcaaa ttatggctct tggtattcca ggaattggtt ttcgtaccga aattcgtcgt ttttatccca gtggacctgt actttcacat attctcggca tggttaatgt tgataatcag ggaatagcgg gtatggaaaa atatatcgat gatgctggct tgagtgattt gcgtgctgct ggtcttgcaa cggaagattc tttaaagcca gttcaacttt cgattgatgt gcgtgttcag acaattgtcc atgatgaact tatgaaggca atgaagcgtt ataaggcgat tgctgcagga gctgttattt taaatatcca cacaagtgaa gttttggctt tggcatcact accagatttt gatcctggaa atcctgntga tgctttgaaa agtgatcgtc tcantcggat gacagctgga acttttgaaa tgggatctat cattaaaagt tttacgaccg cgatggccct tgattcagat atattccatt taaacagtat cattgatgct tcaaaacctc tgcaagcaag tagaggtttt actattcatg attttcatgg gaaaaatcgt ccnttaacgt tatgggaggt ttttatttat tnttctaaca ttggttctgc taaggaggca atggcgatag ggattgatgg acatcgtgct tttttgaaac gacttggttt acttgatcga atgacaacag aattgcctga agttgcccat cctcttgtgc caaatcattg gaaggacatc cattctatga caatttcttt tggacatggt atggcaacaa cgcccttgca aacagcagta gctgctgccg ctttaatgaa tggtggttgg ttaattgctc ctacgttttt aaaacgcaca agagatcaag ctttgcaaca tgcaaaacaa gttttgcagg ctaaaacgag tcaaaatatg cgttatcttt ataaattaaa tagtgatatt ggttctgggc gtaatgcaaa agtagaaggt tatcgcgttg gtggtaagac aggaacagcc gaaaaagttg aaaatggaaa atattctaaa acaaaaaatt tcaatagttt tctcgctgct tttcccattg aagatcctga ttatgttgtt ttaacaatta tcgatgaacc tcagcctgaa gatggaaagt attcagcaac agcagcaatg aatgcagggc cgatacttgc taatattatt cgccgttcag cgaattttct tggaataaaa ccagattttc agaaagaata tgagcctatt ttaaccacag agaacagttt aagtttgtta aaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGACLISYG FEGGQIEEAK GPRVLQLTAR PDILDRNGRL LATDIKTYSL FAEPRRIIDV DETIELLSTV LPDLNWQETY KRLKKKSSFS WIQRGLTPLQ KAQIMALGIP GIGFRTEIRR FYPSGPVLSH ILGMVNVDNQ GIAGMEKYID DAGLSDLRAA GLATEDSLKP VQLSIDVRVQ TIVHDELMKA MKRYKAIAAG AVILNIHTSE VLALASLPDF DPGNPVDALK SDRLNRMTAG TFEMGSIIKS FTTAMALDSD IFHLNSIIDA SKPLQASRGF TIHDFHGKNR PLTLWEVFIY SSNIGSAKEA MAIGIDGHRA FLKRLGLLDR MTTELPEVAH PLVPNHWKDI HSMTISFGHG MATTPLQTAV AAAALMNGGW LIAPTFLKRT RDQALQHAKQ VLQAKTSQNM RYLYKLNSDI GSGRNAKVEG YRVGGKTGTA EKVENGKYSK TKNFNSFLAA FPIEDPDYVV LTIIDEPQPE DGKYSATAAM NAGPILANII RRSANFLGIK PDFQKEYEPI LTTENSLSFV KKR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggggcttgt cttatttctt atgggtttga agggggacag attgaagaag caaaaggacc acgagtcctt caattgactg cacggcctga tattcttgat cgtaatggtc gtttattggc aacagatatt aagacctatt cgctttttgc tgaaccacga cgtattattg atgtcgatga aacaatagaa ttgctctcaa cagttttgcc cgatctcaat tggcaggaaa cttataaacg tcttaaaaaa aagtccagtt tttcttggat tcagcgtggg ttaacgccac tacagaaagc gcaaattatg gctcttggta ttccaggaat tggttttcgt accgaaattc gtcgttttta tcccagtgga cctgtacttt cacatattct cggcatggtt aatgttgata atcagggaat agcgggtatg gaaaaatata tcgatgatgc tggcttgagt gatttgcgtg ctgctggtct tgcaacggaa gattctttaa agccagttca actttcgatt gatgtgcgtg ttcagacaat tgtccatgat gaacttatga aggcaatgaa gcgttataag gcgattgctg caggagctgt tattttaaat atccacacaa gtgaagtttt ggctttggca tcactaccag attttgatcc tggaaatcct gttgatgctt tgaaaagtga tcgtctcaat cggatgacag ctggaacttt tgaaatggga tctatcatta aaagttttac gaccgcgatg gcccttgatt cagatatatt ccatttaaac agtatcattg atgcttcaaa acctctgcaa gcaagtagag gttttactat tcatgatttt catgggaaaa atcgtccctt aacgttatgg gaggttttta tttattcttc taacattggt tctgctaagg aggcaatggc gatagggatt gatggacatc gtgctttttt gaaacgactt ggtttacttg atcgaatgac aacagaattg cctgaagttg cccatcctct tgtgccaaat cattggaagg acatccattc tatgacaatt tcttttggac atggtatggc aacaacgccc ttgcaaacag cagtagctgc tgccgcttta atgaatggtg gttggttaat tgctcctacg tttttaaaac gcacaagaga tcaagctttg caacatgcaa aacaagtttt gcaggctaaa acgagtcaaa atatgcgtta tctttataaa ttaaatagtg atattggttc tgggcgtaat gcaaaagtag aaggttatcg cgttggtggt aagacaggaa cagccgaaaa agttgaaaat ggaaaatatt ctaaaacaaa aaatttcaat agttttctcg ctgcttttcc cattgaagat cctgattatg ttgttttaac aattatcgat gaacctcagc ctgaagatgg aaagtattca gcaacagcag caatgaatgc agggccgata cttgctaata ttattcgccg ttcagcgaat tttcttggaa taaaaccaga ttttcagaaa gaatatgagc ctattttaac cacagagaac agtttaagtt ttgttaaaaa acggaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa