SSGCID: Target Page

BaheA.10252.a

threonyl-tRNA synthetase

CENTER ID:	BaheA.10252.a
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.10252.a.A1.GE30636	Full length( BaheA.10252.a )		1	658

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.819
RefSeq:	YP_033578.1
UniProt:	Q6G3K1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSCSVSLSFP DGSKRDYHSE MTGLELAESI SKSLAKKALA YSLNGIIRDL SDPIKQSGQI EIITREDTRA LELIRHDCAH VLAEAVQELF PGTQVTIGPV IENGFYYDFA RQQPFTLDDL TIIEKKMREI IQRNKPFRKE VWSRKKAREI FSEKGELYKV ELIDSIPDNQ DLKVYYQGDW FDLCRGPHMQ STGQIGNAFK LMKVAGAYWR GDANNPMLTR IYGTAFANEN DLKAYLHMLE EAEKRDHRRL GREMNLFHFQ EEGPGMIFWH KKGWKMFQNL INYMRRRLDD HQYAEVNAPQ VLDRSLWEIS GHWEWYKENM FKAIPAAEDW DNENVYALKP MNCPGHVQIF KHGLKSYRDL PTRIAEFGVV HRYEPSGSLH GLMRVRCFTQ DDAHIFCTDE QLADECLNIN DLILSTYADF GFKEISLKLS TRPEKRVGSD ALWDHAENIM ESVLKTIETK SAGQIKTSIL PGEGAFYGPK FEYTLKDAIG REWQCGTTQL DFNLPERFNA FYIDKDSEKR QPVMIHRAIF GSMERFLGIL IENFSGHMPL WFAPEQIVVA TITSEANEYA KKLTAKLKSV GLSTTLDLRN EKINYKIREH SLQKVPVILV CGKRESETNS VNMRRLGSTE QIFLSVEEVI EQLKNEATPP DLRRVINA

NT Sequence

ATGTCTTGCT CTGTTTCTCT TTCTTTTCCC GATGGTTCGA AGCGTGATTA CCACTCCGAA ATGACTGGTT TAGAATTGGC TGAATCTATT TCTAAATCAC TTGCAAAAAA AGCACTCGCC TATAGCCTTA ACGGAATCAT CCGCGATCTA TCCGATCCAA TAAAACAATC TGGTCAGATA GAAATCATTA CCCGCGAAGA TACACGTGCT CTTGAGCTCA TACGCCATGA TTGTGCGCAT GTCCTAGCCG AAGCAGTACA GGAGCTCTTT CCTGGAACGC AAGTGACAAT TGGCCCTGTT ATTGAAAATG GTTTTTATTA TGATTTTGCA CGTCAACAAC CTTTTACATT GGATGATCTT ACTATCATTG AAAAGAAAAT GCGTGAAATT ATCCAACGCA ATAAACCTTT TAGAAAAGAA GTTTGGTCTC GTAAAAAAGC CAGAGAAATT TTTTCTGAAA AAGGTGAACT TTATAAGGTT GAGCTTATCG ATAGCATCCC CGATAATCAA GATTTGAAAG TCTATTATCA AGGTGATTGG TTTGATCTTT GCCGTGGTCC ACATATGCAA TCTACTGGAC AAATTGGGAA TGCTTTCAAA CTGATGAAAG TTGCAGGAGC TTATTGGCGT GGAGATGCCA ATAATCCAAT GCTAACGAGA ATTTATGGTA CAGCATTTGC AAACGAAAAT GATTTAAAAG CCTATCTACA CATGCTAGAA GAAGCTGAAA AACGTGATCA TCGGCGCTTG GGACGTGAAA TGAACTTATT TCATTTTCAA GAAGAAGGAC CAGGCATGAT TTTTTGGCAC AAAAAAGGCT GGAAAATGTT TCAAAATTTG ATCAACTATA TGCGAAGACG TCTCGATGAT CATCAATATG CCGAAGTTAA TGCACCGCAA GTTCTCGATA GATCACTATG GGAAATATCT GGTCATTGGG AATGGTACAA GGAAAATATG TTCAAAGCAA TTCCCGCAGC TGAAGATTGG GATAATGAAA ATGTTTACGC TCTTAAACCA ATGAATTGTC CTGGTCATGT GCAGATTTTT AAACATGGTT TAAAATCTTA CCGCGATTTG CCAACAAGAA TCGCTGAATT TGGTGTTGTT CATCGTTATG AACCCTCAGG TTCTCTTCAT GGTCTTATGC GTGTACGGTG CTTTACACAA GATGATGCGC ATATTTTTTG TACTGATGAA CAATTAGCTG ATGAATGTCT TAATATTAAT GATCTGATCT TATCAACTTA TGCTGATTTT GGTTTTAAAG AAATCAGTCT TAAACTTTCA ACACGCCCAG AAAAGCGTGT CGGATCTGAC GCATTATGGG ATCATGCAGA AAATATTATG GAATCTGTTC TTAAAACTAT TGAGACAAAA TCCGCAGGGC AAATCAAAAC AAGTATTCTT CCAGGTGAAG GTGCATTTTA TGGACCAAAG TTCGAATATA CATTGAAAGA TGCTATCGGT CGTGAATGGC AATGTGGAAC AACGCAACTA GATTTTAATC TTCCCGAACG CTTCAATGCA TTTTATATTG ATAAAGACTC AGAAAAGCGT CAGCCAGTTA TGATTCATCG TGCCATTTTT GGATCAATGG AGCGTTTTCT TGGTATATTA ATCGAAAATT TTTCTGGACA TATGCCGCTT TGGTTTGCAC CTGAACAAAT TGTTGTAGCA ACAATTACAT CCGAAGCAAA TGAATATGCA AAAAAATTAA CAGCAAAGCT TAAATCCGTT GGATTATCTA CAACATTAGA TCTTCGCAAT GAAAAAATTA ACTATAAAAT ACGTGAGCAC TCTTTACAAA AAGTTCCTGT AATTTTGGTA TGTGGTAAAC GTGAATCTGA AACCAATAGC GTGAATATGC GCCGTTTAGG AAGTACAGAG CAAATCTTCT TGTCTGTAGA AGAAGTGATT GAGCAACTTA AGAATGAGGC AACCCCTCCG GATTTGCGCC GCGTAATAAA TGCC

Details for BaheA.10252.a.A1.GE30636

HARVESTED ON:	10/27/2010
SEQUENCED ON:	11/3/2010
EXPECTED MW:	78kDa
OBSERVED MW:	78kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	23
PERCENT COVERAGE:	28

Validated AA Sequence

MGTLEAXTQG PGSMAEKVAE KNVLAIVGSX XKASVNRQLX EAAAEHAPAG VSXXXXEGLA DLPHYNEDXD GEGAPAAAEA LRAAVADADA VLLVSPXXNG XISSSXKNAX XXLXRPFGSS VFKGKPTVVI GTSAGRYGGX WAIDETRKAA GXXXANVLED VKLAVPXSAX DGKHPREHAE TVXXXXKALD ALTX

Validated NT Sequence

atatgggtac cctggaagct canacccagg gtcctggttc gatggccgag aaagtggcag agaagaacgt gctggcaatc gtcggaagcn tgngtaaggc atcggtcaac cggcagctcg nggaggccgc tgccgaacac gcgccggccg gtgtgagtgn gnccatntan gagggnctgg ctgatctgcc gcactacaac gaggacatng acggtgaggg tgctcccgcc gccgcagagg ccctgcgtgc ggccgtcgcg gatgccgacg cggtgctgct ggtgagtccg gngancaatg gcnccatttc cagctcgntg aagaacgcga tngannggct ctngcgtccc tttggatcga gtgtcttcaa gggcaagccg acagtngtga tcggaacctc ggcgggccgn tatggcggca tntgggcgat cgatgagacc cgcaaggcgg cngggatngn gnccgccaac gtgctngaag acgtcaagct cgccgtgccg tngtcggcgt tngatggcaa gcacccgcgt gagcatgccg agacggtcgn tgannngncc aaggcattgg acgcgttgac cnccgc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSCSVSLSF PDGSKRDYHS EMTGLELAES ISKSLAKKAL AYSLNGIIRD LSDPIKQSGQ IEIITREDTR ALELIRHDCA HVLAEAVQEL FPGTQVTIGP VIENGFYYDF ARQQPFTLDD LTIIEKKMRE IIQRNKPFRK EVWSRKKARE IFSEKGELYK VELIDSIPDN QDLKVYYQGD WFDLCRGPHM QSTGQIGNAF KLMKVAGAYW RGDANNPMLT RIYGTAFANE NDLKAYLHML EEAEKRDHRR LGREMNLFHF QEEGPGMIFW HKKGWKMFQN LINYMRRRLD DHQYAEVNAP QVLDRSLWEI SGHWEWYKEN MFKAIPAAED WDNENVYALK PMNCPGHVQI FKHGLKSYRD LPTRIAEFGV VHRYEPSGSL HGLMRVRCFT QDDAHIFCTD EQLADECLNI NDLILSTYAD FGFKEISLKL STRPEKRVGS DALWDHAENI MESVLKTIET KSAGQIKTSI LPGEGAFYGP KFEYTLKDAI GREWQCGTTQ LDFNLPERFN AFYIDKDSEK RQPVMIHRAI FGSMERFLGI LIENFSGHMP LWFAPEQIVV ATITSEANEY AKKLTAKLKS VGLSTTLDLR NEKINYKIRE HSLQKVPVIL VCGKRESETN SVNMRRLGST EQIFLSVEEV IEQLKNEATP PDLRRVINA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcttgctct gtttctcttt cttttcccga tggttcgaag cgtgattacc actccgaaat gactggttta gaattggctg aatctatttc taaatcactt gcaaaaaaag cactcgccta tagccttaac ggaatcatcc gcgatctatc cgatccaata aaacaatctg gtcagataga aatcattacc cgcgaagata cacgtgctct tgagctcata cgccatgatt gtgcgcatgt cctagccgaa gcagtacagg agctctttcc tggaacgcaa gtgacaattg gccctgttat tgaaaatggt ttttattatg attttgcacg tcaacaacct tttacattgg atgatcttac tatcattgaa aagaaaatgc gtgaaattat ccaacgcaat aaacctttta gaaaagaagt ttggtctcgt aaaaaagcca gagaaatttt ttctgaaaaa ggtgaacttt ataaggttga gcttatcgat agcatccccg ataatcaaga tttgaaagtc tattatcaag gtgattggtt tgatctttgc cgtggtccac atatgcaatc tactggacaa attgggaatg ctttcaaact gatgaaagtt gcaggagctt attggcgtgg agatgccaat aatccaatgc taacgagaat ttatggtaca gcatttgcaa acgaaaatga tttaaaagcc tatctacaca tgctagaaga agctgaaaaa cgtgatcatc ggcgcttggg acgtgaaatg aacttatttc attttcaaga agaaggacca ggcatgattt tttggcacaa aaaaggctgg aaaatgtttc aaaatttgat caactatatg cgaagacgtc tcgatgatca tcaatatgcc gaagttaatg caccgcaagt tctcgataga tcactatggg aaatatctgg tcattgggaa tggtacaagg aaaatatgtt caaagcaatt cccgcagctg aagattggga taatgaaaat gtttacgctc ttaaaccaat gaattgtcct ggtcatgtgc agatttttaa acatggttta aaatcttacc gcgatttgcc aacaagaatc gctgaatttg gtgttgttca tcgttatgaa ccctcaggtt ctcttcatgg tcttatgcgt gtacggtgct ttacacaaga tgatgcgcat attttttgta ctgatgaaca attagctgat gaatgtctta atattaatga tctgatctta tcaacttatg ctgattttgg ttttaaagaa atcagtctta aactttcaac acgcccagaa aagcgtgtcg gatctgacgc attatgggat catgcagaaa atattatgga atctgttctt aaaactattg agacaaaatc cgcagggcaa atcaaaacaa gtattcttcc aggtgaaggt gcattttatg gaccaaagtt cgaatataca ttgaaagatg ctatcggtcg tgaatggcaa tgtggaacaa cgcaactaga ttttaatctt cccgaacgct tcaatgcatt ttatattgat aaagactcag aaaagcgtca gccagttatg attcatcgtg ccatttttgg atcaatggag cgttttcttg gtatattaat cgaaaatttt tctggacata tgccgctttg gtttgcacct gaacaaattg ttgtagcaac aattacatcc gaagcaaatg aatatgcaaa aaaattaaca gcaaagctta aatccgttgg attatctaca acattagatc ttcgcaatga aaaaattaac tataaaatac gtgagcactc tttacaaaaa gttcctgtaa ttttggtatg tggtaaacgt gaatctgaaa ccaatagcgt gaatatgcgc cgtttaggaa gtacagagca aatcttcttg tctgtagaag aagtgattga gcaacttaag aatgaggcaa cccctccgga tttgcgccgc gtaataaatg ccaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa