BaheA.10201.a

methionyl-tRNA synthetase

CENTER ID: BaheA.10201.a
ORGANISM: Bartonella henselae str. Houston-1
ASSOCIATED DISEASE: Bacillary angiomatosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.10201.a.A1.GE27642 Full length( BaheA.10201.a ) 1 519
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaheA.10201.a.A1.PW27505 Full length( BaheA.10201.a ) 1 519

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|283166.5.peg.897
RefSeq: YP_033653.1
UniProt: A0A0H3LWQ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDTYYITTP IFYPNGAPHI GHAYSAIASD VLARFQRLDG KNVFFLSGTD EHGLKMQQTA VALGITPKQL TDRNSAVFQK MLAALNCSNN DFIRTTEKRH YQACQEIWKK MKANGDIYLS RYTGWYSVRQ EAYYEEKDTE VGEDNIRREK ELGSPVEWNE EESYFFKLSR YEKALLEHYE KHPDFIAPPE RRNEVISFIK SGLKDISISR ASFNWGICVP NDSKHVMYVW VDALTNYLSA IGFFDENSKK RAFWPASVHI IGKDIIRFHA IYWPAFLMSA GIALPKRIFA HGFLLNNGVK MSKSIGNVVD PFEMIDHYGL DQVRYFLLRE VPFGQDGSYS HDSFVNRINA DLANDLGNLA QRSLSMIAKN CDAKVPLPAA FLVQDEQLLE KSLQTLDTMR KAMSSQEIHL ALSAIFSVVA DANRYFANEQ PWSLRKNNPE RFSTVLYITL EILRRIGIML LPFMPQSAAK LLDSLKISQE DRLLQHISNS KIQEGLDLPP PEPIFPRYIL KKDDIHHVD
NT Sequence
ATGCCTGACA CATATTATAT AACAACTCCA ATTTTTTATC CTAATGGTGC TCCACACATT GGACACGCCT ATAGTGCAAT TGCAAGTGAC GTTTTGGCCC GTTTTCAACG CTTAGATGGT AAAAATGTAT TTTTTCTCTC TGGTACGGAT GAGCACGGTT TAAAGATGCA ACAAACAGCC GTAGCGTTGG GCATTACACC TAAGCAACTT ACAGACCGCA ACAGTGCTGT ATTTCAAAAA ATGCTTGCAG CATTAAATTG TTCTAACAAT GATTTTATTC GTACAACAGA AAAGCGCCAT TATCAAGCTT GTCAAGAAAT TTGGAAAAAA ATGAAAGCAA ATGGTGATAT TTATCTTAGT CGGTATACGG GTTGGTATTC AGTCCGCCAA GAAGCTTATT ATGAAGAGAA GGATACTGAA GTTGGAGAAG ATAATATCCG TCGCGAAAAG GAATTAGGTT CTCCAGTTGA ATGGAACGAA GAAGAAAGTT ATTTTTTTAA ACTTTCCCGT TATGAAAAAG CTCTTCTTGA ACATTATGAA AAACATCCTG ATTTTATTGC TCCACCTGAA CGACGTAATG AAGTTATAAG TTTTATAAAG TCGGGTTTAA AAGATATTTC CATTTCACGT GCAAGTTTTA ATTGGGGAAT TTGTGTTCCA AATGATTCAA AACATGTAAT GTATGTCTGG GTTGATGCAC TCACGAACTA TCTTTCAGCT ATTGGCTTTT TTGATGAAAA TTCAAAAAAA CGTGCTTTTT GGCCTGCAAG TGTTCATATC ATTGGTAAAG ATATTATTCG TTTTCACGCA ATTTATTGGC CAGCATTTTT AATGTCTGCT GGCATTGCAT TGCCTAAGCG TATTTTTGCA CATGGTTTTT TACTCAATAA TGGTGTAAAA ATGTCAAAGT CCATTGGAAA TGTCGTTGAT CCTTTTGAAA TGATTGATCA CTATGGTCTT GATCAGGTAC GTTATTTTCT TCTTCGTGAA GTGCCGTTTG GACAAGATGG TAGTTATAGC CATGATAGTT TTGTGAATCG CATTAATGCA GATCTTGCCA ATGACCTTGG TAATTTAGCG CAACGCTCTT TATCTATGAT TGCCAAAAAC TGTGATGCAA AAGTCCCTTT ACCAGCTGCA TTTTTAGTTC AAGATGAACA ACTTTTGGAA AAATCTCTTC AAACCCTTGA CACTATGCGC AAAGCCATGT CTTCTCAAGA AATACATTTA GCACTTTCAG CTATTTTTTC AGTCGTTGCA GATGCTAACC GCTATTTTGC AAACGAACAA CCTTGGAGTT TAAGGAAAAA TAATCCAGAA AGGTTTTCTA CAGTTCTTTA CATAACTTTA GAAATCTTAC GGCGAATAGG AATAATGCTG TTGCCTTTTA TGCCACAATC AGCAGCAAAA CTTCTTGATA GCCTTAAGAT TTCTCAAGAA GATCGATTAT TGCAGCATAT AAGCAATTCA AAAATTCAAG AAGGTCTTGA TCTTCCACCA CCAGAACCAA TCTTTCCCCG TTATATTCTT AAGAAAGACG ATATTCACCA TGTTGAT
Details for BaheA.10201.a.A1.GE27642
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 61kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPDTYYITT PIFYPNGAPH IGHAYSAIAS DVLARFQRLD GKNVFFLSGT DEHGLKMQQT AVALGITPKQ LTDRNSAVFQ KMLAALNCSN NDFIRTTEKR HYQACQEIWK KMKANGDIYL SRYTGWYSVR QEAYYEEKDT EVGEDNIRRE KELGSPVEWN EEESYFFKLS RYEKALLEHY EKHPDFIAPP ERRNEVISFI KSGLKDISIS RASFNWGICV PNDSKHVMXV WVDALTNYLS AIGFFDENSK KRAFWPASVH IIGKDIIRFH AIYWPAFLMS AGIALPKRIF AHGFLLNNGV KMSKSIGNVV DPFEMIDHYG LDQVRYFLLR EVPFGQDGSY SHDSFVNRIN ADLANDLGNL AQRSLSMIAK NCDAKVPLPA AFLVQDEQLL EKSLQTLDTM RKAMSSQEIH LALSAIFSVV ADANRYFANE QPWSLRKNNP ERFSTVLYIT LEILRRIGIM LLPFMPQSAA KLLDSLKISQ EDRLLQHISN SKIQEGLDLP PPEPIFPRYI LKKDDIHHVD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctg acacatatta tataacaact ccaatttttt atcctaatgg tgctccacac attggacacg cctatagtgc aattgcaagt gacgttttgg cccgttttca acgcttagat ggtaaaaatg tattttttct ctctggtacg gatgagcacg gtttaaagat gcaacaaaca gccgtagcgt tgggcattac acctaagcaa cttacagacc gcaacagtgc tgtatttcaa aaaatgcttg cagcattaaa ttgttctaac aatgatttta ttcgtacaac agaaaagcgc cattatcaag cttgtcaaga aatttggaaa aaaatgaaag caaatggtga tatttatctt agtcggtata cgggttggta ttcagtccgc caagaagctt attatgaaga gaaggatact gaagttggag aagataatat ccgtcgcgaa aaggaattag gttctccagt tgaatggaac gaagaagaaa gttatttttt taaactttcc cgttatgaaa aagctcttct tgaacattat gaaaaacatc ctgattttat tgctccacct gaacgacgta atgaagttat aagttttata aagtcgggtt taaaagatat ttccatttca cgtgcaagtt ttaattgggg aatttgtgtt ccaaatgatt caaaacatgt aatgnatgtc tgggttgatg cactcacgaa ctatctttca gctattggct tttttgatga aaattcaaaa aaacgtgctt tttggcctgc aagtgttcat atcattggta aagatattat tcgttttcac gcaatttatt ggccagcatt tttaatgtct gctggcattg cattgcctaa gcgtattttt gcacatggtt ttttactcaa taatggtgta aaaatgtcaa agtccattgg aaatgtcgtt gatccttttg aaatgattga tcactatggt cttgatcagg tacgttattt tcttcttcgt gaagtgccgt ttggacaaga tggtagttat agccatgata gttttgtgaa tcgcattaat gcagatcttg ccaatgacct tggtaattta gcgcaacgct ctttatctat gattgccaaa aactgtgatg caaaagtccc tttaccagct gcatttttag ttcaagatga acaacttttg gaaaaatctc ttcaaaccct tgacactatg cgcaaagcca tgtcttctca agaaatacat ttagcacttt cagctatttt ttcagtcgtt gcagatgcta accgctattt tgcaaacgaa caaccttgga gtttaaggaa aaataatcca gaaaggtttt ctacagttct ttacataact ttagaaatct tacggcgaat aggaataatg ctgttgcctt ttatgccaca atcagcagca aaacttcttg atagccttaa gatttctcaa gaagatcgat tattgcagca tataagcaat tcaaaaattc aagaaggtct tgatcttcca ccaccagaac caatctttcc ccgttatatt cttaagaaag acgatattca ccatgttgat taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPDTYYITT PIFYPNGAPH IGHAYSAIAS DVLARFQRLD GKNVFFLSGT DEHGLKMQQT AVALGITPKQ LTDRNSAVFQ KMLAALNCSN NDFIRTTEKR HYQACQEIWK KMKANGDIYL SRYTGWYSVR QEAYYEEKDT EVGEDNIRRE KELGSPVEWN EEESYFFKLS RYEKALLEHY EKHPDFIAPP ERRNEVISFI KSGLKDISIS RASFNWGICV PNDSKHVMYV WVDALTNYLS AIGFFDENSK KRAFWPASVH IIGKDIIRFH AIYWPAFLMS AGIALPKRIF AHGFLLNNGV KMSKSIGNVV DPFEMIDHYG LDQVRYFLLR EVPFGQDGSY SHDSFVNRIN ADLANDLGNL AQRSLSMIAK NCDAKVPLPA AFLVQDEQLL EKSLQTLDTM RKAMSSQEIH LALSAIFSVV ADANRYFANE QPWSLRKNNP ERFSTVLYIT LEILRRIGIM LLPFMPQSAA KLLDSLKISQ EDRLLQHISN SKIQEGLDLP PPEPIFPRYI LKKDDIHHVD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctgacaca tattatataa caactccaat tttttatcct aatggtgctc cacacattgg acacgcctat agtgcaattg caagtgacgt tttggcccgt tttcaacgct tagatggtaa aaatgtattt tttctctctg gtacggatga gcacggttta aagatgcaac aaacagccgt agcgttgggc attacaccta agcaacttac agaccgcaac agtgctgtat ttcaaaaaat gcttgcagca ttaaattgtt ctaacaatga ttttattcgt acaacagaaa agcgccatta tcaagcttgt caagaaattt ggaaaaaaat gaaagcaaat ggtgatattt atcttagtcg gtatacgggt tggtattcag tccgccaaga agcttattat gaagagaagg atactgaagt tggagaagat aatatccgtc gcgaaaagga attaggttct ccagttgaat ggaacgaaga agaaagttat ttttttaaac tttcccgtta tgaaaaagct cttcttgaac attatgaaaa acatcctgat tttattgctc cacctgaacg acgtaatgaa gttataagtt ttataaagtc gggtttaaaa gatatttcca tttcacgtgc aagttttaat tggggaattt gtgttccaaa tgattcaaaa catgtaatgt atgtctgggt tgatgcactc acgaactatc tttcagctat tggctttttt gatgaaaatt caaaaaaacg tgctttttgg cctgcaagtg ttcatatcat tggtaaagat attattcgtt ttcacgcaat ttattggcca gcatttttaa tgtctgctgg cattgcattg cctaagcgta tttttgcaca tggtttttta ctcaataatg gtgtaaaaat gtcaaagtcc attggaaatg tcgttgatcc ttttgaaatg attgatcact atggtcttga tcaggtacgt tattttcttc ttcgtgaagt gccgtttgga caagatggta gttatagcca tgatagtttt gtgaatcgca ttaatgcaga tcttgccaat gaccttggta atttagcgca acgctcttta tctatgattg ccaaaaactg tgatgcaaaa gtccctttac cagctgcatt tttagttcaa gatgaacaac ttttggaaaa atctcttcaa acccttgaca ctatgcgcaa agccatgtct tctcaagaaa tacatttagc actttcagct attttttcag tcgttgcaga tgctaaccgc tattttgcaa acgaacaacc ttggagttta aggaaaaata atccagaaag gttttctaca gttctttaca taactttaga aatcttacgg cgaataggaa taatgctgtt gccttttatg ccacaatcag cagcaaaact tcttgatagc cttaagattt ctcaagaaga tcgattattg cagcatataa gcaattcaaa aattcaagaa ggtcttgatc ttccaccacc agaaccaatc tttccccgtt atattcttaa gaaagacgat attcaccatg ttgataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BaheA.10201.a.A1.PW27505
PURIFICATION DATe: 11/9/2009
CONCENTRATION: 20.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPDTYYITT PIFYPNGAPH IGHAYSAIAS DVLARFQRLD GKNVFFLSGT DEHGLKMQQT AVALGITPKQ LTDRNSAVFQ KMLAALNCSN NDFIRTTEKR HYQACQEIWK KMKANGDIYL SRYTGWYSVR QEAYYEEKDT EVGEDNIRRE KELGSPVEWN EEESYFFKLS RYEKALLEHY EKHPDFIAPP ERRNEVISFI KSGLKDISIS RASFNWGICV PNDSKHVMXV WVDALTNYLS AIGFFDENSK KRAFWPASVH IIGKDIIRFH AIYWPAFLMS AGIALPKRIF AHGFLLNNGV KMSKSIGNVV DPFEMIDHYG LDQVRYFLLR EVPFGQDGSY SHDSFVNRIN ADLANDLGNL AQRSLSMIAK NCDAKVPLPA AFLVQDEQLL EKSLQTLDTM RKAMSSQEIH LALSAIFSVV ADANRYFANE QPWSLRKNNP ERFSTVLYIT LEILRRIGIM LLPFMPQSAA KLLDSLKISQ EDRLLQHISN SKIQEGLDLP PPEPIFPRYI LKKDDIHHVD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctg acacatatta tataacaact ccaatttttt atcctaatgg tgctccacac attggacacg cctatagtgc aattgcaagt gacgttttgg cccgttttca acgcttagat ggtaaaaatg tattttttct ctctggtacg gatgagcacg gtttaaagat gcaacaaaca gccgtagcgt tgggcattac acctaagcaa cttacagacc gcaacagtgc tgtatttcaa aaaatgcttg cagcattaaa ttgttctaac aatgatttta ttcgtacaac agaaaagcgc cattatcaag cttgtcaaga aatttggaaa aaaatgaaag caaatggtga tatttatctt agtcggtata cgggttggta ttcagtccgc caagaagctt attatgaaga gaaggatact gaagttggag aagataatat ccgtcgcgaa aaggaattag gttctccagt tgaatggaac gaagaagaaa gttatttttt taaactttcc cgttatgaaa aagctcttct tgaacattat gaaaaacatc ctgattttat tgctccacct gaacgacgta atgaagttat aagttttata aagtcgggtt taaaagatat ttccatttca cgtgcaagtt ttaattgggg aatttgtgtt ccaaatgatt caaaacatgt aatgnatgtc tgggttgatg cactcacgaa ctatctttca gctattggct tttttgatga aaattcaaaa aaacgtgctt tttggcctgc aagtgttcat atcattggta aagatattat tcgttttcac gcaatttatt ggccagcatt tttaatgtct gctggcattg cattgcctaa gcgtattttt gcacatggtt ttttactcaa taatggtgta aaaatgtcaa agtccattgg aaatgtcgtt gatccttttg aaatgattga tcactatggt cttgatcagg tacgttattt tcttcttcgt gaagtgccgt ttggacaaga tggtagttat agccatgata gttttgtgaa tcgcattaat gcagatcttg ccaatgacct tggtaattta gcgcaacgct ctttatctat gattgccaaa aactgtgatg caaaagtccc tttaccagct gcatttttag ttcaagatga acaacttttg gaaaaatctc ttcaaaccct tgacactatg cgcaaagcca tgtcttctca agaaatacat ttagcacttt cagctatttt ttcagtcgtt gcagatgcta accgctattt tgcaaacgaa caaccttgga gtttaaggaa aaataatcca gaaaggtttt ctacagttct ttacataact ttagaaatct tacggcgaat aggaataatg ctgttgcctt ttatgccaca atcagcagca aaacttcttg atagccttaa gatttctcaa gaagatcgat tattgcagca tataagcaat tcaaaaattc aagaaggtct tgatcttcca ccaccagaac caatctttcc ccgttatatt cttaagaaag acgatattca ccatgttgat taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPDTYYITT PIFYPNGAPH IGHAYSAIAS DVLARFQRLD GKNVFFLSGT DEHGLKMQQT AVALGITPKQ LTDRNSAVFQ KMLAALNCSN NDFIRTTEKR HYQACQEIWK KMKANGDIYL SRYTGWYSVR QEAYYEEKDT EVGEDNIRRE KELGSPVEWN EEESYFFKLS RYEKALLEHY EKHPDFIAPP ERRNEVISFI KSGLKDISIS RASFNWGICV PNDSKHVMYV WVDALTNYLS AIGFFDENSK KRAFWPASVH IIGKDIIRFH AIYWPAFLMS AGIALPKRIF AHGFLLNNGV KMSKSIGNVV DPFEMIDHYG LDQVRYFLLR EVPFGQDGSY SHDSFVNRIN ADLANDLGNL AQRSLSMIAK NCDAKVPLPA AFLVQDEQLL EKSLQTLDTM RKAMSSQEIH LALSAIFSVV ADANRYFANE QPWSLRKNNP ERFSTVLYIT LEILRRIGIM LLPFMPQSAA KLLDSLKISQ EDRLLQHISN SKIQEGLDLP PPEPIFPRYI LKKDDIHHVD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTGACACA TATTATATAA CAACTCCAAT TTTTTATCCT AATGGTGCTC CACACATTGG ACACGCCTAT AGTGCAATTG CAAGTGACGT TTTGGCCCGT TTTCAACGCT TAGATGGTAA AAATGTATTT TTTCTCTCTG GTACGGATGA GCACGGTTTA AAGATGCAAC AAACAGCCGT AGCGTTGGGC ATTACACCTA AGCAACTTAC AGACCGCAAC AGTGCTGTAT TTCAAAAAAT GCTTGCAGCA TTAAATTGTT CTAACAATGA TTTTATTCGT ACAACAGAAA AGCGCCATTA TCAAGCTTGT CAAGAAATTT GGAAAAAAAT GAAAGCAAAT GGTGATATTT ATCTTAGTCG GTATACGGGT TGGTATTCAG TCCGCCAAGA AGCTTATTAT GAAGAGAAGG ATACTGAAGT TGGAGAAGAT AATATCCGTC GCGAAAAGGA ATTAGGTTCT CCAGTTGAAT GGAACGAAGA AGAAAGTTAT TTTTTTAAAC TTTCCCGTTA TGAAAAAGCT CTTCTTGAAC ATTATGAAAA ACATCCTGAT TTTATTGCTC CACCTGAACG ACGTAATGAA GTTATAAGTT TTATAAAGTC GGGTTTAAAA GATATTTCCA TTTCACGTGC AAGTTTTAAT TGGGGAATTT GTGTTCCAAA TGATTCAAAA CATGTAATGT ATGTCTGGGT TGATGCACTC ACGAACTATC TTTCAGCTAT TGGCTTTTTT GATGAAAATT CAAAAAAACG TGCTTTTTGG CCTGCAAGTG TTCATATCAT TGGTAAAGAT ATTATTCGTT TTCACGCAAT TTATTGGCCA GCATTTTTAA TGTCTGCTGG CATTGCATTG CCTAAGCGTA TTTTTGCACA TGGTTTTTTA CTCAATAATG GTGTAAAAAT GTCAAAGTCC ATTGGAAATG TCGTTGATCC TTTTGAAATG ATTGATCACT ATGGTCTTGA TCAGGTACGT TATTTTCTTC TTCGTGAAGT GCCGTTTGGA CAAGATGGTA GTTATAGCCA TGATAGTTTT GTGAATCGCA TTAATGCAGA TCTTGCCAAT GACCTTGGTA ATTTAGCGCA ACGCTCTTTA TCTATGATTG CCAAAAACTG TGATGCAAAA GTCCCTTTAC CAGCTGCATT TTTAGTTCAA GATGAACAAC TTTTGGAAAA ATCTCTTCAA ACCCTTGACA CTATGCGCAA AGCCATGTCT TCTCAAGAAA TACATTTAGC ACTTTCAGCT ATTTTTTCAG TCGTTGCAGA TGCTAACCGC TATTTTGCAA ACGAACAACC TTGGAGTTTA AGGAAAAATA ATCCAGAAAG GTTTTCTACA GTTCTTTACA TAACTTTAGA AATCTTACGG CGAATAGGAA TAATGCTGTT GCCTTTTATG CCACAATCAG CAGCAAAACT TCTTGATAGC CTTAAGATTT CTCAAGAAGA TCGATTATTG CAGCATATAA GCAATTCAAA AATTCAAGAA GGTCTTGATC TTCCACCACC AGAACCAATC TTTCCCCGTT ATATTCTTAA GAAAGACGAT ATTCACCATG TTGATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA