SSGCID: Target Page

BaheA.10100.c

Serine protease

CENTER ID:	BaheA.10100.c
ORGANISM:	Bartonella henselae str. Houston-1
ASSOCIATED DISEASE:	Bacillary angiomatosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.10100.c.A2.GE27641	Mature protein( BaheA.10100.c )		36	505

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaheA.10100.c.A2.PS00407	Mature protein( BaheA.10100.c )		36	505

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283166.5.peg.1144
RefSeq:	YP_033869.1
UniProt:	A0A0H3LXQ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGRNVIGKNT SMKCFLIRAF VCVLLFLLGS MNSSWAVETK NRSLSVPDLA AQLLETVVNI STAQTVDGTE QDEHTSVPVI PEDSLLQEYF TDFFSPKDGQ KESQFQKVRS LGSGFVIDAQ KGLIVTNYHV IVDADDIEVN FTDGTKLKAK LLGKDSKTDL ALLQVDAGSK KLKAVRFGDS EKARIGDWVM AIGNPYGFGG SVTVGIISAR NRDLNAGPYD NFIQTDAAIN RGNSGGPLFD RNGEVIGINT AIVSPSGGSI GIGFAIPSDM ALSVINQLRD FGEIRRGWLA IRIQPVTEDI AKSLKLESPV GALVAGKMEQ TDVDNSQLQI GDIILSFGNA KIKHARDLPR LVAESLEGKV VDITVLRNGQ EKTVKVKLGR LIETDTNENM VEESDNERSG PISKSGTMQF MGMTLSELTA DLRQHYSITD KLQGLVVISV AKNSAADKKR IRTGEVIVDI NQSTVTTIGE AKKRIHKLRE AGRKNALFII ARPDGELRFV TIPMD

NT Sequence

ATGGGTAGAA ATGTCATAGG TAAAAATACG TCTATGAAGT GCTTTCTTAT AAGAGCTTTT GTTTGCGTGC TTTTATTTTT ATTAGGATCA ATGAATTCTT CTTGGGCGGT GGAGACAAAA AATAGATCGC TTTCCGTTCC TGATCTCGCT GCTCAGCTTT TGGAGACAGT TGTAAATATT TCTACAGCAC AGACAGTTGA TGGGACCGAA CAGGATGAAC ATACTTCGGT ACCCGTTATT CCAGAAGATT CATTGTTACA AGAATATTTT ACTGATTTTT TCTCTCCTAA AGATGGTCAA AAAGAGAGTC AGTTTCAAAA AGTACGTTCT TTGGGGTCTG GTTTTGTAAT TGATGCCCAA AAAGGGCTTA TCGTTACAAA TTATCATGTT ATCGTTGATG CTGATGATAT TGAAGTGAAT TTTACCGATG GTACAAAGCT AAAGGCAAAA TTACTTGGAA AGGATAGCAA GACTGATTTG GCACTGCTTC AAGTGGATGC AGGAAGTAAA AAATTAAAGG CTGTACGTTT TGGTGACTCA GAAAAGGCCC GTATTGGTGA TTGGGTGATG GCTATTGGTA ATCCTTATGG TTTTGGTGGA AGTGTAACGG TTGGTATTAT TTCTGCACGT AATCGTGATC TCAATGCTGG TCCTTATGAT AATTTTATTC AGACAGATGC AGCGATTAAC CGAGGTAATT CTGGTGGTCC GTTATTTGAT AGAAATGGTG AGGTTATTGG AATTAATACA GCAATTGTTT CGCCTTCTGG AGGGTCTATT GGTATTGGTT TTGCTATACC ATCGGATATG GCGCTTTCTG TTATCAATCA ATTGCGTGAT TTTGGAGAAA TAAGGCGTGG TTGGTTGGCT ATTCGTATTC AGCCGGTGAC AGAGGATATT GCAAAAAGTT TAAAATTAGA AAGTCCTGTA GGGGCGTTGG TTGCAGGTAA AATGGAACAA ACAGATGTAG ATAATAGTCA GTTGCAGATT GGCGATATCA TTCTTTCCTT TGGCAACGCT AAAATTAAAC ATGCACGTGA CTTGCCTCGT TTGGTGGCGG AAAGTCTGGA AGGAAAGGTA GTGGATATTA CTGTTTTGCG CAATGGGCAA GAAAAAACAG TAAAAGTTAA GCTTGGACGT TTAATTGAGA CAGATACAAA TGAGAATATG GTAGAAGAAT CTGATAATGA AAGAAGCGGT CCTATATCGA AAAGCGGGAC AATGCAATTT ATGGGAATGA CATTATCTGA GCTTACAGCG GATTTACGTC AACATTATTC TATTACGGAT AAACTTCAGG GATTGGTCGT GATATCTGTT GCCAAAAATA GTGCGGCGGA TAAAAAGCGT ATTCGCACTG GTGAAGTGAT TGTTGATATC AATCAAAGTA CTGTCACGAC AATTGGTGAG GCTAAAAAAC GTATTCATAA ATTACGTGAA GCCGGACGCA AAAATGCTCT CTTCATCATT GCTAGGCCTG ATGGAGAATT ACGATTCGTA ACGATTCCAA TGGAT

Details for BaheA.10100.c.A2.GE27641

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	53kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAVETKNRS LSVPDLAAQL LETVVNISTA QTVDGTEQDE HTSVPVIPED SLLQEYFTDF FSPKDGQKES QFQKVRSLGS GFVIDAQKGL IVTNYHVIVD ADDIEVNFTD GTKLKAKLLG KDSKTDLALL QVDAGSKKLK AVRFGDSEKA RIGDWVMAIG NPYGFGGSVT VGIISARNRD LNAGPYDNFI QTDAAINRXN SGGPLFDRNG EVIGINTAIV SPSGGSIGIG FAIPXDMALS VINQLRDFGE IRRGWLAXXI QPVTEDIAKS LKLESPVGAL VAGKMEQTDV DNSQLQIGDI ILSFGNAKIK HARDLPRLVA ESLEGKVVDI TVLRNGQEKT VKVKLGRLIE TDTNENMVEE SDNERSGPIS KSGTMQFMGM TLSELTADLR QHYSITDKLQ GLVVISVAKN SAADKKRIRT GEVIVDINQS TVTTIGEAKK RIHKLREAGR KNALFIIARP DGELRFVTI

Validated NT Sequence

agaaggagnn ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggtggag acaaaaaata gatcgctttc cgttcctgat ctcgctgctc agcttttgga gacagttgta aatatttcta cagcacagac agttgatggg accgaacagg atgaacatac ttcggtaccc gttattccag aagattcatt gttacaagaa tattttactg attttttctc tcctaaagat ggtcaaaaag agagtcagtt tcaaaaagta cgttctttgg ggtctggttt tgtaattgat gcccaaaaag ggcttatcgt tacaaattat catgttatcg ttgatgctga tgatattgaa gtgaatttta ccgatggtac aaagctaaag gcaaaattac ttggaaagga tagcaagact gatttggcac tgcttcaagt ggatgcagga agtaaaaaat taaaggctgt acgttttggt gactcagaaa aggcccgtat tggtgattgg gtgatggcta ttggtaatcc ttatggtttt ggtggaagtg taacggttgg tattatttct gcacgtaatc gtgatctcaa tgctggtcct tatgataatt ttattcagac agatgcagcg attaaccgan gtaattctgg tggtccgtta tttgatagaa atggtgaggt tattggaatt aatacagcaa ttgtttcgcc ttctggaggg tctattggta ttggttttgc tataccatng gatatggcgc tttctgttat caatcaattg cgtgattttg gagaaataag gcgtggttgg ttggctantn gtattcagcc ggtgacagag gatattgcaa aaagtttaaa attagaaagt cctgtagggg cgttggttgc aggtaaaatg gaacaaacag atgtagataa tagtcagttg cagattggcg atatcattct ttcctttggc aacgctaaaa ttaaacatgc acgtgacttg cctcgtttgg tggcggaaag tctggaagga aaggtagtgg atattactgt tttgcgcaat gggcaagaaa aaacagtaaa agttaagctt ggacgtttaa ttgagacaga tacaaatgag aatatggtag aagaatctga taatgaaaga agcggtccta tatcgaaaag cgggacaatg caatttatgg gaatgacatt atctgagctt acagcggatt tacgtcaaca ttattctatt acggataaac ttcagggatt ggtcgtgata tctgttgcca aaaatagtgc ggcggataaa aagcgtattc gcactggtga agtgattgtt gatatcaatc aaagtactgt cacgacaatt ggtgaggcta aaaaacgtat tcataaatta cgtgaagccg gacgcaaaaa tgctctcttc atcattgcta ggcctgatgg agaattacga ttcgtaacga ttcca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAVETKNRS LSVPDLAAQL LETVVNISTA QTVDGTEQDE HTSVPVIPED SLLQEYFTDF FSPKDGQKES QFQKVRSLGS GFVIDAQKGL IVTNYHVIVD ADDIEVNFTD GTKLKAKLLG KDSKTDLALL QVDAGSKKLK AVRFGDSEKA RIGDWVMAIG NPYGFGGSVT VGIISARNRD LNAGPYDNFI QTDAAINRGN SGGPLFDRNG EVIGINTAIV SPSGGSIGIG FAIPSDMALS VINQLRDFGE IRRGWLAIRI QPVTEDIAKS LKLESPVGAL VAGKMEQTDV DNSQLQIGDI ILSFGNAKIK HARDLPRLVA ESLEGKVVDI TVLRNGQEKT VKVKLGRLIE TDTNENMVEE SDNERSGPIS KSGTMQFMGM TLSELTADLR QHYSITDKLQ GLVVISVAKN SAADKKRIRT GEVIVDINQS TVTTIGEAKK RIHKLREAGR KNALFIIARP DGELRFVTIP MD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggtggag acaaaaaata gatcgctttc cgttcctgat ctcgctgctc agcttttgga gacagttgta aatatttcta cagcacagac agttgatggg accgaacagg atgaacatac ttcggtaccc gttattccag aagattcatt gttacaagaa tattttactg attttttctc tcctaaagat ggtcaaaaag agagtcagtt tcaaaaagta cgttctttgg ggtctggttt tgtaattgat gcccaaaaag ggcttatcgt tacaaattat catgttatcg ttgatgctga tgatattgaa gtgaatttta ccgatggtac aaagctaaag gcaaaattac ttggaaagga tagcaagact gatttggcac tgcttcaagt ggatgcagga agtaaaaaat taaaggctgt acgttttggt gactcagaaa aggcccgtat tggtgattgg gtgatggcta ttggtaatcc ttatggtttt ggtggaagtg taacggttgg tattatttct gcacgtaatc gtgatctcaa tgctggtcct tatgataatt ttattcagac agatgcagcg attaaccgag gtaattctgg tggtccgtta tttgatagaa atggtgaggt tattggaatt aatacagcaa ttgtttcgcc ttctggaggg tctattggta ttggttttgc tataccatcg gatatggcgc tttctgttat caatcaattg cgtgattttg gagaaataag gcgtggttgg ttggctattc gtattcagcc ggtgacagag gatattgcaa aaagtttaaa attagaaagt cctgtagggg cgttggttgc aggtaaaatg gaacaaacag atgtagataa tagtcagttg cagattggcg atatcattct ttcctttggc aacgctaaaa ttaaacatgc acgtgacttg cctcgtttgg tggcggaaag tctggaagga aaggtagtgg atattactgt tttgcgcaat gggcaagaaa aaacagtaaa agttaagctt ggacgtttaa ttgagacaga tacaaatgag aatatggtag aagaatctga taatgaaaga agcggtccta tatcgaaaag cgggacaatg caatttatgg gaatgacatt atctgagctt acagcggatt tacgtcaaca ttattctatt acggataaac ttcagggatt ggtcgtgata tctgttgcca aaaatagtgc ggcggataaa aagcgtattc gcactggtga agtgattgtt gatatcaatc aaagtactgt cacgacaatt ggtgaggcta aaaaacgtat tcataaatta cgtgaagccg gacgcaaaaa tgctctcttc atcattgcta ggcctgatgg agaattacga ttcgtaacga ttccaatgga taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for BaheA.10100.c.A2.PS00407

PURIFICATION DATe:	11/12/2009
CONCENTRATION:	13.38mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAVETKNRS LSVPDLAAQL LETVVNISTA QTVDGTEQDE HTSVPVIPED SLLQEYFTDF FSPKDGQKES QFQKVRSLGS GFVIDAQKGL IVTNYHVIVD ADDIEVNFTD GTKLKAKLLG KDSKTDLALL QVDAGSKKLK AVRFGDSEKA RIGDWVMAIG NPYGFGGSVT VGIISARNRD LNAGPYDNFI QTDAAINRGN SGGPLFDRNG EVIGINTAIV SPSGGSIGIG FAIPSDMALS VINQLRDFGE IRRGWLAIRI QPVTEDIAKS LKLESPVGAL VAGKMEQTDV DNSQLQIGDI ILSFGNAKIK HARDLPRLVA ESLEGKVVDI TVLRNGQEKT VKVKLGRLIE TDTNENMVEE SDNERSGPIS KSGTMQFMGM TLSELTADLR QHYSITDKLQ GLVVISVAKN SAADKKRIRT GEVIVDINQS TVTTIGEAKK RIHKLREAGR KNALFIIARP DGELRFVTIP MD

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGTGGAG ACAAAAAATA GATCGCTTTC CGTTCCTGAT CTCGCTGCTC AGCTTTTGGA GACAGTTGTA AATATTTCTA CAGCACAGAC AGTTGATGGG ACCGAACAGG ATGAACATAC TTCGGTACCC GTTATTCCAG AAGATTCATT GTTACAAGAA TATTTTACTG ATTTTTTCTC TCCTAAAGAT GGTCAAAAAG AGAGTCAGTT TCAAAAAGTA CGTTCTTTGG GGTCTGGTTT TGTAATTGAT GCCCAAAAAG GGCTTATCGT TACAAATTAT CATGTTATCG TTGATGCTGA TGATATTGAA GTGAATTTTA CCGATGGTAC AAAGCTAAAG GCAAAATTAC TTGGAAAGGA TAGCAAGACT GATTTGGCAC TGCTTCAAGT GGATGCAGGA AGTAAAAAAT TAAAGGCTGT ACGTTTTGGT GACTCAGAAA AGGCCCGTAT TGGTGATTGG GTGATGGCTA TTGGTAATCC TTATGGTTTT GGTGGAAGTG TAACGGTTGG TATTATTTCT GCACGTAATC GTGATCTCAA TGCTGGTCCT TATGATAATT TTATTCAGAC AGATGCAGCG ATTAACCGAG GTAATTCTGG TGGTCCGTTA TTTGATAGAA ATGGTGAGGT TATTGGAATT AATACAGCAA TTGTTTCGCC TTCTGGAGGG TCTATTGGTA TTGGTTTTGC TATACCATCG GATATGGCGC TTTCTGTTAT CAATCAATTG CGTGATTTTG GAGAAATAAG GCGTGGTTGG TTGGCTATTC GTATTCAGCC GGTGACAGAG GATATTGCAA AAAGTTTAAA ATTAGAAAGT CCTGTAGGGG CGTTGGTTGC AGGTAAAATG GAACAAACAG ATGTAGATAA TAGTCAGTTG CAGATTGGCG ATATCATTCT TTCCTTTGGC AACGCTAAAA TTAAACATGC ACGTGACTTG CCTCGTTTGG TGGCGGAAAG TCTGGAAGGA AAGGTAGTGG ATATTACTGT TTTGCGCAAT GGGCAAGAAA AAACAGTAAA AGTTAAGCTT GGACGTTTAA TTGAGACAGA TACAAATGAG AATATGGTAG AAGAATCTGA TAATGAAAGA AGCGGTCCTA TATCGAAAAG CGGGACAATG CAATTTATGG GAATGACATT ATCTGAGCTT ACAGCGGATT TACGTCAACA TTATTCTATT ACGGATAAAC TTCAGGGATT GGTCGTGATA TCTGTTGCCA AAAATAGTGC GGCGGATAAA AAGCGTATTC GCACTGGTGA AGTGATTGTT GATATCAATC AAAGTACTGT CACGACAATT GGTGAGGCTA AAAAACGTAT TCATAAATTA CGTGAAGCCG GACGCAAAAA TGCTCTCTTC ATCATTGCTA GGCCTGATGG AGAATTACGA TTCGTAACGA TTCCAATGGA TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA